RchiOBHm_Chr7g0194321

Calcineurin B-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
7
OG0017662
Physical Location & Seq
Forward (+)
12427568 .. 12429058
1491 bp
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UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 636 bp
ATGGGCTGTGTCTGCATGAAGCAGAGACTTGAATGTGAAAACTCAACTGTTCTTGCTTCTCAAACCTATTTCAAGGTGAATGAGATAGAAAATTTGTATGAGCTGTTCAGGAAGTTAAGCAGTTCTATAGTCGATGATGGGCTTATAAGCAAAGAAGAGTTTCAGCTCGGATTGTTCAGGAACAGCAAAACACAGAGTCTCTTTGCAGACAGGATCTTCGACATGTTTGATTCAAAGCACGACGGGGTAATAGAATTCGAAGAGTTTGTTAAATGTCTAAGTGTCTTCCACCCTGAAGCACCAAGAGCAGAAAAAGTGTCATTTGCATTTCACTTGTACGATATATGGCAGACGGGATTCATTGAACGCGATGAGGTCAAAGAAATGGTTATGGCGCTTCTGAAAGAGTCAGACTTGATCTTATCTGACGACATTGTTGAAGCCATTCTTGATAAGACATTCAAGGAAGCGGATTTAAATGGAGATGGAAAGATTGATCTGGAAGAGTGGAACCAACTCGTGGATCAAAATCCATCCTTATTGAAGAACATGACAATTCCATACCTCAAGGACATCACAACTGCTTTTCATAGTTTTGTGCTGAGATCAGACATTGAAGATGACACCAAGTACTAA

Protein Analysis

211

Amino Acids

24.48

Weight (kDa)

4.55

Isoelectric Point (pI)

35.57

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EF-hand_7 PF13499 45 - 91 8.1e-06 EF-hand domain pair
EF-hand_1 PF00036 70 - 94 3.1e-06 EF hand domain
EF-hand_7 PF13499 106 - 169 2.9e-09 EF-hand domain pair
EF-hand_1 PF00036 150 - 174 9.8e-07 EF hand domain
EF-hand_5 PF13202 150 - 174 4.1e-09 EF hand
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0017662)

Species Orthologous Gene IDs
fragaria_vesca FvH4_5g04810
malus_domestica MD14G1130200.v1.1
prunus_persica Prupe.5G122100_v2.0.a1 Prupe.5G122100_v2.0.a1
rosa_chinensis RchiOBHm_Chr7g0194321
rosa_multiflora Rmu_sc0000901.1_g000015
rosa_roxburghii Rroxscaffold_3G00261420
rosa_rugosa Rorug07G0015400
rosa_samantha Rh7AG142400 Rh7CG146500 Rh7DG144100
rosa_wichuraiana Rw7G012190

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 146
AccB7I CCANNNNNTGG 1 cut(s) 520
AccII CGCG 1 cut(s) 369
AciI CCGC 1 cut(s) 470
AclWI GGATC 2 cut(s) 221, 531
AcsI RAATTY 2 cut(s) 91, 254
AcuI CTGAAG 1 cut(s) 315
AfaI GTAC 2 cut(s) 338, 632
AfiI CCNNNNNNNGG 1 cut(s) 520
AflIII ACRYGT 1 cut(s) 222
AgsI TTSAA 8 cut(s) 32, 73, 234, 365, 440, 463, 544, 617
AluBI AGCT 2 cut(s) 103, 166
AluI AGCT 2 cut(s) 103, 166
Alw26I GTCTC 2 cut(s) 19, 203
AlwI GGATC 2 cut(s) 221, 531
ApoI RAATTY 2 cut(s) 91, 254
ArsI GACNNNNNNTTYG 2 cut(s) 200, 232
Asp700I GAANNNNTTC 2 cut(s) 159, 444
AspLEI GCGC 1 cut(s) 397
AsuHPI GGTGA 1 cut(s) 88
AsuII TTCGAA 1 cut(s) 258
BauI CACGAG 1 cut(s) 518
BbsI GAAGAC 1 cut(s) 277
BccI CCATC 3 cut(s) 131, 479, 541
BcoDI GTCTC 2 cut(s) 19, 203
BfmI CTRYAG 1 cut(s) 126
BfoI RGCGCY 1 cut(s) 398
BmcAI AGTACT 1 cut(s) 632
BmiI GGNNCC 1 cut(s) 512
BpiI GAAGAC 1 cut(s) 277
Bpu14I TTCGAA 1 cut(s) 258
BpuEI CTTGAG 1 cut(s) 551
BsaBI GATNNNNATC 1 cut(s) 528
Bsc4I CCNNNNNNNGG 1 cut(s) 520
Bse8I GATNNNNATC 1 cut(s) 528
BseGI GGATG 1 cut(s) 533
BseJI GATNNNNATC 1 cut(s) 528
BseLI CCNNNNNNNGG 1 cut(s) 520
BseMII CTCAG 1 cut(s) 593
Bsh1236I CGCG 1 cut(s) 369
BslI CCNNNNNNNGG 1 cut(s) 520
BsmAI GTCTC 2 cut(s) 19, 203
Bsp119I TTCGAA 1 cut(s) 258
Bsp143I GATC 5 cut(s) 213, 417, 496, 523, 605
BspACI CCGC 1 cut(s) 470
BspCNI CTCAG 1 cut(s) 594
BspFNI CGCG 1 cut(s) 369
BspLI GGNNCC 1 cut(s) 512
BspPI GGATC 2 cut(s) 221, 531
BspT104I TTCGAA 1 cut(s) 258
BssMI GATC 5 cut(s) 213, 417, 496, 523, 605
BssSI CACGAG 1 cut(s) 518
Bst2BI CACGAG 1 cut(s) 518
Bst4CI ACNGT 1 cut(s) 49
Bst6I CTCTTC 3 cut(s) 150, 255, 498
BstBI TTCGAA 1 cut(s) 258
BstDEI CTNAG 2 cut(s) 278, 602
BstF5I GGATG 1 cut(s) 533
BstFNI CGCG 1 cut(s) 369
BstH2I RGCGCY 1 cut(s) 398
BstHHI GCGC 1 cut(s) 397
BstKTI GATC 5 cut(s) 216, 420, 499, 526, 608
BstMAI GTCTC 2 cut(s) 19, 203
BstMBI GATC 5 cut(s) 213, 417, 496, 523, 605
BstMWI GCNNNNNNNGC 2 cut(s) 12, 305
BstNSI RCATGY 1 cut(s) 226
BstSFI CTRYAG 1 cut(s) 126
BstUI CGCG 1 cut(s) 369
BstV2I GAAGAC 1 cut(s) 277
BstX2I RGATCY 1 cut(s) 213
BstYI RGATCY 1 cut(s) 213
BtgZI GCGATG 1 cut(s) 384
BtsCI GGATG 1 cut(s) 533
CfoI GCGC 1 cut(s) 397
Csp6I GTAC 2 cut(s) 337, 631
CviAII CATG 3 cut(s) 16, 223, 550
CviJI RGCY 5 cut(s) 6, 103, 142, 166, 443
CviKI_1 RGCY 5 cut(s) 6, 103, 142, 166, 443
CviQI GTAC 2 cut(s) 337, 631
DdeI CTNAG 2 cut(s) 278, 602
DpnI GATC 5 cut(s) 215, 419, 498, 525, 607
DpnII GATC 5 cut(s) 213, 417, 496, 523, 605
DraI TTTAAA 1 cut(s) 477
Eam1104I CTCTTC 3 cut(s) 150, 255, 498
EarI CTCTTC 3 cut(s) 150, 255, 498
Eco57I CTGAAG 1 cut(s) 315
EcoRI GAATTC 1 cut(s) 254
FaeI CATG 3 cut(s) 19, 226, 553
FalI AAGNNNNNCTT 2 cut(s) 432, 464
FatI CATG 3 cut(s) 15, 222, 549
FokI GGATG 1 cut(s) 520
GlaI GCGC 1 cut(s) 396
HaeII RGCGCY 1 cut(s) 398
HhaI GCGC 1 cut(s) 397
Hin1II CATG 3 cut(s) 19, 226, 553
Hin6I GCGC 1 cut(s) 395
HinP1I GCGC 1 cut(s) 395
HinfI GANTC 4 cut(s) 196, 230, 357, 407
HphI GGTGA 1 cut(s) 88
Hpy188I TCNGA 5 cut(s) 170, 402, 412, 427, 610
Hpy188III TCNNGA 4 cut(s) 109, 178, 449, 500
Hpy99I CGWCG 1 cut(s) 245
HpyCH4III ACNGT 1 cut(s) 49
HpyCH4V TGCA 3 cut(s) 15, 206, 326
HpyF10VI GCNNNNNNNGC 2 cut(s) 12, 305
HpyF3I CTNAG 2 cut(s) 278, 602
Hsp92II CATG 3 cut(s) 19, 226, 553
HspAI GCGC 1 cut(s) 395
Kzo9I GATC 5 cut(s) 213, 417, 496, 523, 605
LpnPI CCDG 5 cut(s) 94, 163, 196, 306, 485
MalI GATC 5 cut(s) 215, 419, 498, 525, 607
MboI GATC 5 cut(s) 213, 417, 496, 523, 605
MboII GAAGA 7 cut(s) 167, 208, 272, 277, 515, 556, 629
MflI RGATCY 1 cut(s) 213
MluCI AATT 3 cut(s) 91, 254, 555
MlyI GAGTC 2 cut(s) 205, 416
MnlI CCTC 2 cut(s) 367, 575
MroXI GAANNNNTTC 2 cut(s) 159, 444
MseI TTAA 3 cut(s) 116, 270, 476
MvnI CGCG 1 cut(s) 369
MwoI GCNNNNNNNGC 2 cut(s) 12, 305
NdeII GATC 5 cut(s) 213, 417, 496, 523, 605
NlaIII CATG 3 cut(s) 19, 226, 553
NlaIV GGNNCC 1 cut(s) 512
NspI RCATGY 1 cut(s) 226
NspV TTCGAA 1 cut(s) 258
PciI ACATGT 1 cut(s) 222
PdmI GAANNNNTTC 2 cut(s) 159, 444
PfeI GAWTC 2 cut(s) 230, 357
PflMI CCANNNNNTGG 1 cut(s) 520
PleI GAGTC 2 cut(s) 204, 415
PpsI GAGTC 2 cut(s) 204, 415
PscI ACATGT 1 cut(s) 222
PsiI TTATAA 1 cut(s) 146
PspN4I GGNNCC 1 cut(s) 512
PsrI GAACNNNNNNTAC 2 cut(s) 89, 121
PsuI RGATCY 1 cut(s) 213
RsaI GTAC 2 cut(s) 338, 632
RsaNI GTAC 2 cut(s) 337, 631
SaqAI TTAA 3 cut(s) 116, 270, 476
Sau3AI GATC 5 cut(s) 213, 417, 496, 523, 605
ScaI AGTACT 1 cut(s) 632
SchI GAGTC 2 cut(s) 205, 416
SetI ASST 6 cut(s) 68, 78, 105, 168, 378, 567
SfcI CTRYAG 1 cut(s) 126
SfuI TTCGAA 1 cut(s) 258
SmiI ATTTAAAT 1 cut(s) 477
SmlI CTYRAG 1 cut(s) 566
SmoI CTYRAG 1 cut(s) 566
Sse9I AATT 3 cut(s) 91, 254, 555
SsiI CCGC 1 cut(s) 470
SwaI ATTTAAAT 1 cut(s) 477
TaaI ACNGT 1 cut(s) 49
TaqI TCGA 3 cut(s) 132, 219, 258
TasI AATT 3 cut(s) 91, 254, 555
TatI WGTACW 1 cut(s) 630
TfiI GAWTC 2 cut(s) 230, 357
Tru1I TTAA 3 cut(s) 116, 270, 476
Tru9I TTAA 3 cut(s) 116, 270, 476
TspDTI ATGAA 3 cut(s) 32, 349, 578
Van91I CCANNNNNTGG 1 cut(s) 520
XapI RAATTY 2 cut(s) 91, 254
XceI RCATGY 1 cut(s) 226
XmnI GAANNNNTTC 2 cut(s) 159, 444
ZrmI AGTACT 1 cut(s) 632
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.