RchiOBHm_Chr7g0203711

Oxidoreductase family, C-terminal alpha/beta domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
7
OG0014074
Physical Location & Seq
Forward (+)
21365884 .. 21369153
3270 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1197 bp
ATGGTGTTAAATGCCGACCAAAGTAACCTCAATAAAATCCAAACTACCACAGTAGAGAGAGACAGAGTAGAGGAAAAGAAAAAATTGTCAGCTATGGCTGTTGCTTCAAACGTAGTCAAGTATGGGATTGTAGGGGTGGGTATGATGGGTCGAGAGCACCTCATCAATTTGCACCATCTTCGCTCGGAAGGCGTGGCCGTGGTAGCCATAGCTGACCCCCATGTTCCTTCCCAAAAACTCGCCTTGGACTTGGCTCACTCTTTCAATTGGCCTCTAAAGGTTTTTTCAGGGCATAAGGAGCTGTTGGATAGTCTTCTCTGTGATGTGGTGGTTGTGTCAACTCCAAATATGACCCATCATCGTATTCTAATGGATATAATTAACCACCCCAAACCCCACCATGTACTGGTGGAGAAGCCGTTGTGTACCACAGTTGCTCACTGCAAAGAGGTTATTGAAGCCGCTAGAAAGAGGCAGGATATGTTGGTGCAAGTTGGACTGGAGTATAGATACATGCCGCCGGTTGCTAAACTGATAAACATAGTAAAGGGTGGAAGTCTTGGCCAGGTCAAAATGGTGGCAATCCGAGAACACAGGTTTCCTTTCTTGGTTAAGGTCAACAATTGGAACCGGTTTAATGTTAACTCTGGGGGGACACTGGTAGAGAAGTGCTGTCACTTCTTTGATTTGATGAGGCTGTTTGCTGGAGCTAATCCTTTTCGTGTGATGGCTTCTGGAGCCATTGATGTTAACCACAAAGATGAAATATATGATGGGAAGGTACCAGATATAATTGACAATGCATATGTTATTGTCGAATTTGATAATGGTGCTCGTGGGATGCTTGACCTTTGTATGTTTGCTGAAGGGAGTAAAAATGAGCAAGAAATATCGGTGGTTGGTGATGTCGGAAAGGGAGAGGCCTTTGTTCCTGAGAGTATTGTACGTACTGGTACTCGAGTAGGTGGGAGAGATGGTGTCAAGACTTTAAAAGCTGAGAATGATCAGATAAAATATGATGGGCTGCATCATGGATCTAGCTATTTGGAACACCTGCACTTCTTATCTGCAATTAGGGCTAAACAAGGACCTGCTGTTGATTTGCATGATGGATTAGTTTCAGTTGCCATTGGAGTTGCAGCACAACTTTCCATAGAGAAAGGCCGGTTTGTTACAATTGAAGAAGTCATGAATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
GFO_IDH_MocA GFO_IDH_MocA_C

Protein Analysis

398

Amino Acids

43.96

Weight (kDa)

7.05

Isoelectric Point (pI)

28.91

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GFO_IDH_MocA PF01408 40 - 166 7.7e-27 Oxidoreductase family, NAD-binding Rossmann fold
GFO_IDH_MocA_C3 PF22725 176 - 304 8.3e-15 GFO/IDH/MocA C-terminal domain
GFO_IDH_MocA_C PF02894 182 - 392 4e-40 Oxidoreductase family, C-terminal alpha/beta domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014074)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G17370
fragaria_vesca FvH4_5g19170 FvH4_5g19170
malus_domestica MD04G1062400.v1.1
prunus_persica Prupe.5G066000_v2.0.a1 Prupe.5G066000_v2.0.a1 Prupe.5G066000_v2.0.a1
pyrus_communis pycom04g05590
rosa_chinensis RchiOBHm_Chr7g0203711
rosa_laevigata RLG00000003511
rosa_multiflora Rmu_sc0001714.1_g000005 Rmu_sc0005354.1_g000002
rosa_roxburghii Rroxscaffold_3G00253600
rosa_rugosa Rorug07G0078100.1
rosa_samantha Rh7BG207000 Rh7DG214900
rosa_wichuraiana Rw7G018120

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 1062
Acc36I ACCTGC 2 cut(s) 1062, 1099
Acc65I GGTACC 1 cut(s) 781
AccB1I GGYRCC 1 cut(s) 781
AccB7I CCANNNNNTGG 1 cut(s) 406
AciI CCGC 2 cut(s) 462, 518
AclWI GGATC 1 cut(s) 1042
AcoI YGGCCR 2 cut(s) 195, 562
AcsI RAATTY 1 cut(s) 818
AcuI CTGAAG 1 cut(s) 885
AfaI GTAC 6 cut(s) 405, 427, 783, 945, 949, 955
AfiI CCNNNNNNNGG 1 cut(s) 406
AgeI ACCGGT 1 cut(s) 630
AgsI TTSAA 4 cut(s) 108, 265, 458, 1181
AjnI CCWGG 1 cut(s) 564
AluBI AGCT 6 cut(s) 92, 212, 301, 710, 995, 1041
AluI AGCT 6 cut(s) 92, 212, 301, 710, 995, 1041
Alw21I GWGCWC 2 cut(s) 159, 835
Alw26I GTCTC 1 cut(s) 54
AlwI GGATC 1 cut(s) 1042
Ama87I CYCGRG 1 cut(s) 957
AoxI GGCC 5 cut(s) 195, 269, 562, 921, 1162
ApeKI GCWGC 2 cut(s) 1024, 1139
ApoI RAATTY 1 cut(s) 818
AsiGI ACCGGT 1 cut(s) 630
Asp718I GGTACC 1 cut(s) 781
AspS9I GGNCC 1 cut(s) 1088
AsuHPI GGTGA 1 cut(s) 914
AvaI CYCGRG 1 cut(s) 957
AvaII GGWCC 1 cut(s) 1088
BaeI ACNNNNGTAYC 2 cut(s) 945, 978
BalI TGGCCA 1 cut(s) 564
BanI GGYRCC 1 cut(s) 781
BauI CACGAG 1 cut(s) 834
BbsI GAAGAC 1 cut(s) 305
Bbv12I GWGCWC 2 cut(s) 159, 835
BbvI GCAGC 2 cut(s) 1011, 1151
BccI CCATC 8 cut(s) 139, 183, 363, 721, 767, 968, 1013, 1103
BceAI ACGGC 2 cut(s) 182, 403
BcgI CGANNNNNNTGC 2 cut(s) 161, 195
BciT130I CCWGG 1 cut(s) 566
BclI TGATCA 1 cut(s) 1003
BcoDI GTCTC 1 cut(s) 54
BfaI CTAG 2 cut(s) 465, 1038
BfuAI ACCTGC 2 cut(s) 1062, 1099
BisI GCNGC 4 cut(s) 462, 518, 1025, 1140
BlsI GCNGC 4 cut(s) 463, 519, 1026, 1141
Bme1390I CCNGG 1 cut(s) 566
Bme18I GGWCC 1 cut(s) 1088
BmeT110I CYCGRG 1 cut(s) 957
BmgT120I GGNCC 1 cut(s) 1088
BmiI GGNNCC 3 cut(s) 629, 739, 783
BmrFI CCNGG 1 cut(s) 566
BmsI GCATC 2 cut(s) 831, 1036
BpiI GAAGAC 1 cut(s) 305
BplI GAGNNNNNCTC 2 cut(s) 144, 176
BpmI CTGGAG 3 cut(s) 521, 726, 756
BsaAI YACGTR 1 cut(s) 947
BsaJI CCNNGG 2 cut(s) 198, 243
BsaWI WCCGGW 1 cut(s) 630
BsaXI ACNNNNNCTCC 4 cut(s) 404, 434, 961, 991
Bsc4I CCNNNNNNNGG 1 cut(s) 406
Bse118I RCCGGY 3 cut(s) 520, 630, 1164
Bse1I ACTGG 4 cut(s) 411, 504, 663, 955
BseBI CCWGG 1 cut(s) 566
BseDI CCNNGG 2 cut(s) 198, 243
BseGI GGATG 1 cut(s) 846
BseLI CCNNNNNNNGG 1 cut(s) 406
BseMII CTCAG 2 cut(s) 924, 987
BseNI ACTGG 4 cut(s) 411, 504, 663, 955
BseXI GCAGC 2 cut(s) 1011, 1151
BsgI GTGCAG 1 cut(s) 1040
BshFI GGCC 5 cut(s) 197, 271, 564, 923, 1164
BshNI GGYRCC 1 cut(s) 781
BshTI ACCGGT 1 cut(s) 630
BsiHKAI GWGCWC 2 cut(s) 159, 835
BsiHKCI CYCGRG 1 cut(s) 957
BsiSI CCGG 3 cut(s) 521, 631, 1165
BslFI GGGAC 1 cut(s) 667
BslI CCNNNNNNNGG 1 cut(s) 406
BsmAI GTCTC 1 cut(s) 54
BsmFI GGGAC 1 cut(s) 667
BsnI GGCC 5 cut(s) 197, 271, 564, 923, 1164
BsoBI CYCGRG 1 cut(s) 957
Bsp1286I GDGCHC 2 cut(s) 159, 835
Bsp143I GATC 2 cut(s) 1003, 1034
BspACI CCGC 2 cut(s) 462, 518
BspANI GGCC 5 cut(s) 197, 271, 564, 923, 1164
BspCNI CTCAG 2 cut(s) 925, 988
BspHI TCATGA 1 cut(s) 1188
BspLI GGNNCC 3 cut(s) 629, 739, 783
BspMI ACCTGC 2 cut(s) 1062, 1099
BspPI GGATC 1 cut(s) 1042
BspT107I GGYRCC 1 cut(s) 781
BsrFI RCCGGY 3 cut(s) 520, 630, 1164
BsrI ACTGG 4 cut(s) 411, 504, 663, 955
BssAI RCCGGY 3 cut(s) 520, 630, 1164
BssECI CCNNGG 2 cut(s) 198, 243
BssMI GATC 2 cut(s) 1003, 1034
BssSI CACGAG 1 cut(s) 834
BssT1I CCWWGG 1 cut(s) 243
Bst2BI CACGAG 1 cut(s) 834
Bst2UI CCWGG 1 cut(s) 566
Bst4CI ACNGT 2 cut(s) 52, 433
BstBAI YACGTR 1 cut(s) 947
BstDEI CTNAG 2 cut(s) 933, 996
BstDSI CCRYGG 1 cut(s) 198
BstF5I GGATG 1 cut(s) 846
BstKTI GATC 2 cut(s) 1006, 1037
BstMAI GTCTC 1 cut(s) 54
BstMBI GATC 2 cut(s) 1003, 1034
BstMWI GCNNNNNNNGC 5 cut(s) 189, 203, 298, 737, 1076
BstNI CCWGG 1 cut(s) 566
BstNSI RCATGY 1 cut(s) 517
BstSCI CCNGG 1 cut(s) 564
BstSNI TACGTA 1 cut(s) 947
BstV1I GCAGC 2 cut(s) 1011, 1151
BstV2I GAAGAC 1 cut(s) 305
BstX2I RGATCY 1 cut(s) 1034
BstYI RGATCY 1 cut(s) 1034
BsuRI GGCC 5 cut(s) 197, 271, 564, 923, 1164
BtgI CCRYGG 1 cut(s) 198
BtsCI GGATG 1 cut(s) 846
BtsI GCAGTG 1 cut(s) 439
BtsIMutI CAGTG 2 cut(s) 439, 656
BveI ACCTGC 2 cut(s) 1062, 1099
CciI TCATGA 1 cut(s) 1188
Cfr10I RCCGGY 3 cut(s) 520, 630, 1164
Cfr13I GGNCC 1 cut(s) 1088
Csp6I GTAC 6 cut(s) 404, 426, 782, 944, 948, 954
CspAI ACCGGT 1 cut(s) 630
CviAII CATG 6 cut(s) 221, 401, 514, 1031, 1106, 1189
CviQI GTAC 6 cut(s) 404, 426, 782, 944, 948, 954
DdeI CTNAG 2 cut(s) 933, 996
DpnI GATC 2 cut(s) 1005, 1036
DpnII GATC 2 cut(s) 1003, 1034
DraI TTTAAA 1 cut(s) 990
EaeI YGGCCR 2 cut(s) 195, 562
Eco105I TACGTA 1 cut(s) 947
Eco130I CCWWGG 1 cut(s) 243
Eco147I AGGCCT 1 cut(s) 923
Eco47I GGWCC 1 cut(s) 1088
Eco57I CTGAAG 1 cut(s) 885
Eco88I CYCGRG 1 cut(s) 957
EcoO109I RGGNCCY 1 cut(s) 1088
EcoRII CCWGG 1 cut(s) 564
EcoT14I CCWWGG 1 cut(s) 243
EcoT22I ATGCAT 1 cut(s) 805
ErhI CCWWGG 1 cut(s) 243
FaeI CATG 6 cut(s) 224, 404, 517, 1034, 1109, 1192
FaqI GGGAC 1 cut(s) 667
FatI CATG 6 cut(s) 220, 400, 513, 1030, 1105, 1188
FauNDI CATATG 1 cut(s) 805
FbaI TGATCA 1 cut(s) 1003
Fnu4HI GCNGC 4 cut(s) 462, 518, 1025, 1140
FokI GGATG 1 cut(s) 853
Fsp4HI GCNGC 4 cut(s) 462, 518, 1025, 1140
FspBI CTAG 2 cut(s) 465, 1038
GluI GCNGC 4 cut(s) 462, 518, 1025, 1140
GsuI CTGGAG 3 cut(s) 521, 726, 756
HaeIII GGCC 5 cut(s) 197, 271, 564, 923, 1164
HapII CCGG 3 cut(s) 521, 631, 1165
Hin1II CATG 6 cut(s) 224, 404, 517, 1034, 1109, 1192
HincII GTYRAC 4 cut(s) 339, 619, 643, 751
HindII GTYRAC 4 cut(s) 339, 619, 643, 751
HpaI GTTAAC 2 cut(s) 643, 751
HpaII CCGG 3 cut(s) 521, 631, 1165
HphI GGTGA 1 cut(s) 914
Hpy166II GTNNAC 5 cut(s) 339, 426, 619, 643, 751
Hpy188I TCNGA 4 cut(s) 187, 587, 911, 1008
Hpy188III TCNNGA 5 cut(s) 152, 735, 932, 982, 1189
Hpy8I GTNNAC 5 cut(s) 339, 426, 619, 643, 751
HpyAV CCTTC 4 cut(s) 182, 237, 772, 860
HpyCH4III ACNGT 2 cut(s) 52, 433
HpyCH4IV ACGT 2 cut(s) 111, 946
HpyCH4V TGCA 9 cut(s) 172, 444, 490, 803, 1027, 1057, 1070, 1105, 1139
HpyF10VI GCNNNNNNNGC 5 cut(s) 189, 203, 298, 737, 1076
HpyF3I CTNAG 2 cut(s) 933, 996
HpySE526I ACGT 2 cut(s) 111, 946
Hsp92II CATG 6 cut(s) 224, 404, 517, 1034, 1109, 1192
KpnI GGTACC 1 cut(s) 785
Ksp22I TGATCA 1 cut(s) 1003
KspAI GTTAAC 2 cut(s) 643, 751
Kzo9I GATC 2 cut(s) 1003, 1034
LmnI GCTCC 3 cut(s) 298, 707, 737
Lsp1109I GCAGC 2 cut(s) 1011, 1151
LweI GCATC 2 cut(s) 831, 1036
MaeI CTAG 2 cut(s) 465, 1038
MaeII ACGT 2 cut(s) 111, 946
MaeIII GTNAC 3 cut(s) 23, 674, 1171
MalI GATC 2 cut(s) 1005, 1036
MboI GATC 2 cut(s) 1003, 1034
MboII GAAGA 3 cut(s) 170, 305, 1193
MfeI CAATTG 3 cut(s) 265, 622, 1176
MflI RGATCY 1 cut(s) 1034
MhlI GDGCHC 2 cut(s) 159, 835
MlsI TGGCCA 1 cut(s) 564
MluNI TGGCCA 1 cut(s) 564
MmeI TCCRAC 3 cut(s) 285, 475, 889
MnlI CCTC 8 cut(s) 38, 64, 170, 282, 442, 465, 687, 913
Mox20I TGGCCA 1 cut(s) 564
Mph1103I ATGCAT 1 cut(s) 805
MscI TGGCCA 1 cut(s) 564
MseI TTAA 7 cut(s) 8, 381, 612, 636, 642, 750, 989
MslI CAYNNNNRTG 1 cut(s) 759
Msp20I TGGCCA 1 cut(s) 564
MspI CCGG 3 cut(s) 521, 631, 1165
MspR9I CCNGG 1 cut(s) 566
MunI CAATTG 3 cut(s) 265, 622, 1176
MvaI CCWGG 1 cut(s) 566
MwoI GCNNNNNNNGC 5 cut(s) 189, 203, 298, 737, 1076
NdeI CATATG 1 cut(s) 805
NdeII GATC 2 cut(s) 1003, 1034
NlaIII CATG 6 cut(s) 224, 404, 517, 1034, 1109, 1192
NlaIV GGNNCC 3 cut(s) 629, 739, 783
NmuCI GTSAC 1 cut(s) 674
NsiI ATGCAT 1 cut(s) 805
NspI RCATGY 1 cut(s) 517
PaeR7I CTCGAG 1 cut(s) 957
PagI TCATGA 1 cut(s) 1188
PaqCI CACCTGC 1 cut(s) 1062
PceI AGGCCT 1 cut(s) 923
PflMI CCANNNNNTGG 1 cut(s) 406
PinAI ACCGGT 1 cut(s) 630
PkrI GCNGC 4 cut(s) 463, 519, 1026, 1141
Ppu21I YACGTR 1 cut(s) 947
PpuMI RGGWCCY 1 cut(s) 1088
Psp5II RGGWCCY 1 cut(s) 1088
Psp6I CCWGG 1 cut(s) 564
PspGI CCWGG 1 cut(s) 564
PspN4I GGNNCC 3 cut(s) 629, 739, 783
PspPI GGNCC 1 cut(s) 1088
PspPPI RGGWCCY 1 cut(s) 1088
PspXI VCTCGAGB 1 cut(s) 957
PsuI RGATCY 1 cut(s) 1034
RsaI GTAC 6 cut(s) 405, 427, 783, 945, 949, 955
RsaNI GTAC 6 cut(s) 404, 426, 782, 944, 948, 954
RseI CAYNNNNRTG 1 cut(s) 759
SaqAI TTAA 7 cut(s) 8, 381, 612, 636, 642, 750, 989
SatI GCNGC 4 cut(s) 462, 518, 1025, 1140
Sau3AI GATC 2 cut(s) 1003, 1034
Sau96I GGNCC 1 cut(s) 1088
ScrFI CCNGG 1 cut(s) 566
SduI GDGCHC 2 cut(s) 159, 835
SfaNI GCATC 2 cut(s) 831, 1036
Sfr274I CTCGAG 1 cut(s) 957
SinI GGWCC 1 cut(s) 1088
SlaI CTCGAG 1 cut(s) 957
SmiMI CAYNNNNRTG 1 cut(s) 759
SmlI CTYRAG 1 cut(s) 957
SmoI CTYRAG 1 cut(s) 957
SnaBI TACGTA 1 cut(s) 947
SseBI AGGCCT 1 cut(s) 923
SsiI CCGC 2 cut(s) 462, 518
SspMI CTAG 2 cut(s) 465, 1038
StuI AGGCCT 1 cut(s) 923
StyD4I CCNGG 1 cut(s) 564
StyI CCWWGG 1 cut(s) 243
TaaI ACNGT 2 cut(s) 52, 433
TaiI ACGT 2 cut(s) 114, 949
TaqI TCGA 3 cut(s) 151, 816, 958
TatI WGTACW 1 cut(s) 403
TauI GCSGC 2 cut(s) 464, 520
Tru1I TTAA 7 cut(s) 8, 381, 612, 636, 642, 750, 989
Tru9I TTAA 7 cut(s) 8, 381, 612, 636, 642, 750, 989
TscAI CASTG 2 cut(s) 446, 663
TseFI GTSAC 1 cut(s) 674
TseI GCWGC 2 cut(s) 1024, 1139
Tsp45I GTSAC 1 cut(s) 674
TspDTI ATGAA 1 cut(s) 777
TspRI CASTG 2 cut(s) 446, 663
Van91I CCANNNNNTGG 1 cut(s) 406
VpaK11BI GGWCC 1 cut(s) 1088
XapI RAATTY 1 cut(s) 818
XceI RCATGY 1 cut(s) 517
XhoI CTCGAG 1 cut(s) 957
XspI CTAG 2 cut(s) 465, 1038
Zsp2I ATGCAT 1 cut(s) 805
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.