RchiOBHm_Chr6g0275221

acyl-activating enzyme 1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
6
OG0014332
Physical Location & Seq
Reverse (-)
36897677 .. 36901776
4100 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1665 bp
ATGGAGGGTGCGATTAGGTGTTCCGCCAACTATGTTCCTCTTTCTCCGATCAGCTTCTTGGAGCGCTCAGCCATAGTCTACAGAGACAGACCCTCTGTTGTGTATGGAGACATCGTCTACACTTGGAGACAGACACTTGAACGATGCACCAGACTTGCTTCTGCTCTTGCCCAACTTGGAATTTCTCGAGGCGATGTGGTTGCTGCACTGGCGCCAAATATTCCAGCAATGTATGAGGTCCATTTTGGTGTTCCAATGGCCGGGGCAGTTTTGTGTACACTTAACGTACGTCATGATTCAAAAATGGTTTCAGTATTAATGAAGCATTCAGAAGCCAAAATCATTTTTGTAGACTACCAATTTATCCATGTTGCGAAGGGAGCATTTGATATCTTATCCAAGACTGGAACCAAGGTCCCCCTTCTAGTTTTAATTCCAGAGAGTGATCAATCATCTCCTGACTTCTGCAATCCCCCTTCTGCATACATAGAATATGAGAGACTCTTAGGAAAGGGAAATCTTGGTTTTGAGATCCTACGACCAAAAGACGAATGGGATGCAATTTCGCTCAACTACACTTCAGGCACTACTTCAAGCCCAAAAGGTGTCATTTATAGCCATAGAGGTGCGTATCTCAATTCTCTGGCAGGAGTTATTCTCAATGAGATGGTATCAATGCCTGTATATTTGTGGTGTGTTCCCATGTTTCATTGCAATGGATGGTGCCTCACTTGGGCTGTAGCTGCTCAAGGTGGCACTAATGTCTGCCAAAGAAATGTGACTGCAAAAGGAATTTTCACCAGTATATCTCAGCATCGGGTGACCCACATGGGTGGTGCACCTACTGTCTTAAACATGATTGTAAATGCACCTGAAAATGATCGGAGGCCACTTCCAGGCAAGGTAGTGGTCATGACCGGGGCTGCACCACCACCATCCCAGGTACTGTTCAAAATGGAAGAGCTAGGGTTCAGTGTAACACATGCATATGGTTTGACAGAGACTTATGGTCCTGGGACAGTTTGCGCTTGGAAACCTGAATGGGATTCCCTACCTCGAGATGAACAAGCAAAAATCAAGTCACGACAAGGGTTGCAACATATTGGATTGGAGGAACTTGATGTTAAAGATCCGGTCACGATGAAGAGTGTTCCATCTGATGCAAAAACCATGGGTGAGGTTATGTTCAGGGGCAACACTGTTATGAATGGATATTTGAAAGATCATAAATCAACACAGGATGCATTTGAAGGTGGATGGTTTCGCAGTGGGGACTTGGCGGTCAGACACCCAGATGGTTACATAGAGCTAAAGGATCGTTCCAAGGATATGATAATTTCCGGTGGAGAAAACATTAGCACAATTGAGGTGGAATCAGTGCTTTTCAGGCATCCAGATGTTCTTGAGGCAGCTATTGTGGGAAGGCCTGATGAATATTGGGGGGAGACACCCTGTGCTTTTGTGAAATTGAAGGATGGTTGTAATGCTAGTGCAGAGGATATCATTAAGTTCTGTAGGGATCGGTTACCCCACTACATGGCTCCTCGAACTGTTATATTCGAAGATTTGCCAAAGACTTCAACTGGGAAGGTGCAGAAGTATGTACTGAGAGAGAAAGCTAAGGCCATGGGAAGTCCCTCCAAGACCAGCATCAGCAAACTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005777 GO:0042579 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
AMP-binding AMP-binding_C

Protein Analysis

554

Amino Acids

61.1

Weight (kDa)

7.56

Isoelectric Point (pI)

36.58

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
AMP-binding PF00501 20 - 406 7.8e-74 AMP-binding enzyme
AMP-binding_C PF13193 456 - 530 1.7e-22 AMP-binding enzyme C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014332)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G20560 AT1G20560
fragaria_vesca FvH4_2g13610 FvH4_2g13610 FvH4_2g13610 FvH4_2g13610
malus_domestica MD05G1027100.v1.1
prunus_persica Prupe.8G032600_v2.0.a1 Prupe.8G032600_v2.0.a1
pyrus_communis pycom05g01910
rosa_chinensis RchiOBHm_Chr6g0275221
rosa_laevigata RLG00000013486
rosa_roxburghii Rroxscaffold_7G00193540
rosa_rugosa Rorug06G0090300
rosa_samantha Rh6AG203600 Rh6CG209000
rosa_wichuraiana Rw6G017670

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 1280
AccB1I GGYRCC 2 cut(s) 211, 723
AccB7I CCANNNNNTGG 1 cut(s) 1537
AccI GTMKAC 3 cut(s) 78, 117, 351
AciI CCGC 2 cut(s) 24, 1280
AclWI GGATC 4 cut(s) 526, 1124, 1323, 1527
AcoI YGGCCR 1 cut(s) 258
AcsI RAATTY 2 cut(s) 180, 792
AcuI CTGAAG 1 cut(s) 564
AcyI GRCGYC 1 cut(s) 212
AdeI CACNNNGTG 1 cut(s) 1454
AfaI GTAC 4 cut(s) 277, 288, 945, 1605
AfeI AGCGCT 1 cut(s) 65
AfiI CCNNNNNNNGG 4 cut(s) 260, 733, 896, 1537
AgsI TTSAA 8 cut(s) 140, 300, 594, 952, 1219, 1250, 1471, 1581
AhdI GACNNNNNGTC 1 cut(s) 1008
AjnI CCWGG 3 cut(s) 895, 939, 1012
AleI CACNNNNGTG 1 cut(s) 831
AluBI AGCT 6 cut(s) 54, 743, 964, 1309, 1412, 1619
AluI AGCT 6 cut(s) 54, 743, 964, 1309, 1412, 1619
Alw21I GWGCWC 1 cut(s) 841
Alw26I GTCTC 6 cut(s) 78, 102, 121, 493, 995, 1439
Alw44I GTGCAC 1 cut(s) 837
AlwI GGATC 4 cut(s) 526, 1124, 1323, 1527
AlwNI CAGNNNCTG 1 cut(s) 946
Ama87I CYCGRG 2 cut(s) 186, 1056
Aor51HI AGCGCT 1 cut(s) 65
AoxI GGCC 4 cut(s) 258, 887, 1424, 1623
ApaLI GTGCAC 1 cut(s) 837
ApeKI GCWGC 4 cut(s) 203, 743, 923, 1409
ApoI RAATTY 2 cut(s) 180, 792
ArsI GACNNNNNNTTYG 2 cut(s) 1064, 1096
AseI ATTAAT 1 cut(s) 317
AspLEI GCGC 3 cut(s) 66, 214, 1028
AspS9I GGNCC 3 cut(s) 238, 415, 1010
AsuC2I CCSGG 2 cut(s) 262, 919
AsuHPI GGTGA 3 cut(s) 790, 832, 1187
AsuII TTCGAA 1 cut(s) 1560
AvaI CYCGRG 2 cut(s) 186, 1056
AvaII GGWCC 3 cut(s) 238, 415, 1010
BaeGI GKGCMC 1 cut(s) 841
BanI GGYRCC 2 cut(s) 211, 723
Bbv12I GWGCWC 1 cut(s) 841
BbvI GCAGC 4 cut(s) 190, 730, 910, 1421
BccI CCATC 7 cut(s) 661, 714, 943, 1162, 1251, 1289, 1469
BcgI CGANNNNNNTGC 4 cut(s) 182, 216, 539, 573
BciT130I CCWGG 3 cut(s) 897, 941, 1014
BclI TGATCA 1 cut(s) 445
BcnI CCSGG 2 cut(s) 262, 919
BcoDI GTCTC 6 cut(s) 78, 102, 121, 493, 995, 1439
BfaI CTAG 3 cut(s) 425, 965, 1488
BfmI CTRYAG 3 cut(s) 79, 738, 1513
BfoI RGCGCY 2 cut(s) 67, 215
BisI GCNGC 4 cut(s) 204, 744, 924, 1410
BlpI GCTNAGC 1 cut(s) 67
BlsI GCNGC 4 cut(s) 205, 745, 925, 1411
Bme1390I CCNGG 5 cut(s) 262, 897, 919, 941, 1014
Bme18I GGWCC 3 cut(s) 238, 415, 1010
BmeRI GACNNNNNGTC 1 cut(s) 1008
BmeT110I CYCGRG 2 cut(s) 186, 1056
BmgT120I GGNCC 3 cut(s) 238, 415, 1010
BmiI GGNNCC 5 cut(s) 213, 409, 417, 725, 1542
BmrFI CCNGG 5 cut(s) 262, 897, 919, 941, 1014
BmrI ACTGGG 1 cut(s) 1593
BmsI GCATC 7 cut(s) 134, 547, 823, 1150, 1231, 1399, 1659
BmuI ACTGGG 1 cut(s) 1593
BplI GAGNNNNNCTC 2 cut(s) 642, 674
Bpu10I CCTNAGC 1 cut(s) 1620
Bpu1102I GCTNAGC 1 cut(s) 67
Bpu14I TTCGAA 1 cut(s) 1560
BpuEI CTTGAG 2 cut(s) 732, 1424
BpuMI CCSGG 2 cut(s) 262, 919
BsaHI GRCGYC 1 cut(s) 212
BsaJI CCNNGG 8 cut(s) 261, 411, 918, 939, 1013, 1170, 1323, 1626
BsaWI WCCGGW 2 cut(s) 1132, 1340
BsaXI ACNNNNNCTCC 2 cut(s) 99, 129
Bsc4I CCNNNNNNNGG 4 cut(s) 260, 733, 896, 1537
Bse1I ACTGG 4 cut(s) 213, 409, 801, 1588
Bse3DI GCAATG 3 cut(s) 234, 709, 721
BseBI CCWGG 3 cut(s) 897, 941, 1014
BseDI CCNNGG 8 cut(s) 261, 411, 918, 939, 1013, 1170, 1323, 1626
BseGI GGATG 7 cut(s) 562, 725, 935, 1246, 1262, 1390, 1480
BseLI CCNNNNNNNGG 4 cut(s) 260, 733, 896, 1537
BseMI GCAATG 3 cut(s) 234, 709, 721
BseMII CTCAG 3 cut(s) 81, 824, 1598
BseNI ACTGG 4 cut(s) 213, 409, 801, 1588
BseRI GAGGAG 1 cut(s) 1533
BseSI GKGCMC 1 cut(s) 841
BseXI GCAGC 4 cut(s) 190, 730, 910, 1421
BsgI GTGCAG 4 cut(s) 189, 909, 1512, 1613
BshFI GGCC 4 cut(s) 260, 889, 1426, 1625
BshNI GGYRCC 2 cut(s) 211, 723
BsiHKAI GWGCWC 1 cut(s) 841
BsiHKCI CYCGRG 2 cut(s) 186, 1056
BsiSI CCGG 4 cut(s) 261, 918, 1133, 1341
BsiWI CGTACG 1 cut(s) 286
BslFI GGGAC 4 cut(s) 401, 1030, 1286, 1620
BslI CCNNNNNNNGG 4 cut(s) 260, 733, 896, 1537
BsmAI GTCTC 6 cut(s) 78, 102, 121, 493, 995, 1439
BsmFI GGGAC 4 cut(s) 401, 1030, 1286, 1620
BsmI GAATGC 1 cut(s) 325
BsnI GGCC 4 cut(s) 260, 889, 1426, 1625
BsoBI CYCGRG 2 cut(s) 186, 1056
Bsp119I TTCGAA 1 cut(s) 1560
Bsp1286I GDGCHC 1 cut(s) 841
Bsp1407I TGTACA 1 cut(s) 275
Bsp143I GATC 8 cut(s) 48, 445, 531, 880, 1129, 1222, 1315, 1519
Bsp1720I GCTNAGC 1 cut(s) 67
Bsp19I CCATGG 2 cut(s) 1170, 1626
BspACI CCGC 2 cut(s) 24, 1280
BspANI GGCC 4 cut(s) 260, 889, 1426, 1625
BspCNI CTCAG 3 cut(s) 80, 823, 1599
BspHI TCATGA 2 cut(s) 292, 912
BspLI GGNNCC 5 cut(s) 213, 409, 417, 725, 1542
BspPI GGATC 4 cut(s) 526, 1124, 1323, 1527
BspQI GCTCTTC 1 cut(s) 954
BspT104I TTCGAA 1 cut(s) 1560
BspT107I GGYRCC 2 cut(s) 211, 723
BsrDI GCAATG 3 cut(s) 234, 709, 721
BsrGI TGTACA 1 cut(s) 275
BsrI ACTGG 4 cut(s) 213, 409, 801, 1588
BssECI CCNNGG 8 cut(s) 261, 411, 918, 939, 1013, 1170, 1323, 1626
BssMI GATC 8 cut(s) 48, 445, 531, 880, 1129, 1222, 1315, 1519
BssNI GRCGYC 1 cut(s) 212
BssT1I CCWWGG 4 cut(s) 411, 1170, 1323, 1626
Bst2UI CCWGG 3 cut(s) 897, 941, 1014
Bst4CI ACNGT 6 cut(s) 847, 948, 1021, 1201, 1552, 1662
Bst6I CTCTTC 2 cut(s) 954, 1139
BstACI GRCGYC 1 cut(s) 212
BstAUI TGTACA 1 cut(s) 275
BstBI TTCGAA 1 cut(s) 1560
BstDEI CTNAG 5 cut(s) 67, 505, 810, 1607, 1620
BstDSI CCRYGG 2 cut(s) 1170, 1626
BstEII GGTNACC 2 cut(s) 820, 1524
BstF5I GGATG 7 cut(s) 562, 725, 935, 1246, 1262, 1390, 1480
BstH2I RGCGCY 2 cut(s) 67, 215
BstHHI GCGC 3 cut(s) 66, 214, 1028
BstKTI GATC 8 cut(s) 51, 448, 534, 883, 1132, 1225, 1318, 1522
BstMAI GTCTC 6 cut(s) 78, 102, 121, 493, 995, 1439
BstMBI GATC 8 cut(s) 48, 445, 531, 880, 1129, 1222, 1315, 1519
BstMWI GCNNNNNNNGC 4 cut(s) 209, 380, 743, 1387
BstNI CCWGG 3 cut(s) 897, 941, 1014
BstNSI RCATGY 1 cut(s) 986
BstPI GGTNACC 2 cut(s) 820, 1524
BstSCI CCNGG 5 cut(s) 260, 895, 917, 939, 1012
BstSFI CTRYAG 3 cut(s) 79, 738, 1513
BstSLI GKGCMC 1 cut(s) 841
BstV1I GCAGC 4 cut(s) 190, 730, 910, 1421
BstX2I RGATCY 2 cut(s) 531, 1129
BstXI CCANNNNNNTGG 1 cut(s) 833
BstYI RGATCY 2 cut(s) 531, 1129
BsuRI GGCC 4 cut(s) 260, 889, 1426, 1625
BtgI CCRYGG 2 cut(s) 1170, 1626
BtgZI GCGATG 1 cut(s) 207
BtsCI GGATG 7 cut(s) 562, 725, 935, 1246, 1262, 1390, 1480
BtsI GCAGTG 1 cut(s) 1273
BtsIMutI CAGTG 5 cut(s) 206, 979, 1197, 1273, 1383
CaiI CAGNNNCTG 1 cut(s) 946
CciI TCATGA 2 cut(s) 292, 912
CfoI GCGC 3 cut(s) 66, 214, 1028
Cfr13I GGNCC 3 cut(s) 238, 415, 1010
Csp6I GTAC 4 cut(s) 276, 287, 944, 1604
CspCI CAANNNNNGTGG 2 cut(s) 1350, 1385
CviQI GTAC 4 cut(s) 276, 287, 944, 1604
DdeI CTNAG 5 cut(s) 67, 505, 810, 1607, 1620
DinI GGCGCC 1 cut(s) 213
DpnI GATC 8 cut(s) 50, 447, 533, 882, 1131, 1224, 1317, 1521
DpnII GATC 8 cut(s) 48, 445, 531, 880, 1129, 1222, 1315, 1519
DraIII CACNNNGTG 1 cut(s) 1454
DrdI GACNNNNNNGTC 1 cut(s) 1280
DriI GACNNNNNGTC 1 cut(s) 1008
DseDI GACNNNNNNGTC 1 cut(s) 1280
EaeI YGGCCR 1 cut(s) 258
Eam1104I CTCTTC 2 cut(s) 954, 1139
Eam1105I GACNNNNNGTC 1 cut(s) 1008
EarI CTCTTC 2 cut(s) 954, 1139
EciI GGCGGA 1 cut(s) 13
Eco130I CCWWGG 4 cut(s) 411, 1170, 1323, 1626
Eco147I AGGCCT 1 cut(s) 1426
Eco32I GATATC 2 cut(s) 391, 1501
Eco47I GGWCC 3 cut(s) 238, 415, 1010
Eco47III AGCGCT 1 cut(s) 65
Eco57I CTGAAG 1 cut(s) 564
Eco88I CYCGRG 2 cut(s) 186, 1056
Eco91I GGTNACC 2 cut(s) 820, 1524
EcoO109I RGGNCCY 1 cut(s) 415
EcoO65I GGTNACC 2 cut(s) 820, 1524
EcoRII CCWGG 3 cut(s) 895, 939, 1012
EcoRV GATATC 2 cut(s) 391, 1501
EcoT14I CCWWGG 4 cut(s) 411, 1170, 1323, 1626
EcoT22I ATGCAT 2 cut(s) 988, 1246
EgeI GGCGCC 1 cut(s) 213
EheI GGCGCC 1 cut(s) 213
ErhI CCWWGG 4 cut(s) 411, 1170, 1323, 1626
FaqI GGGAC 4 cut(s) 401, 1030, 1286, 1620
FauNDI CATATG 1 cut(s) 988
FbaI TGATCA 1 cut(s) 445
FblI GTMKAC 3 cut(s) 78, 117, 351
Fnu4HI GCNGC 4 cut(s) 204, 744, 924, 1410
FokI GGATG 7 cut(s) 569, 732, 922, 1253, 1269, 1377, 1487
Fsp4HI GCNGC 4 cut(s) 204, 744, 924, 1410
FspBI CTAG 3 cut(s) 425, 965, 1488
GlaI GCGC 3 cut(s) 65, 213, 1027
GluI GCNGC 4 cut(s) 204, 744, 924, 1410
HaeII RGCGCY 2 cut(s) 67, 215
HaeIII GGCC 4 cut(s) 260, 889, 1426, 1625
HapII CCGG 4 cut(s) 261, 918, 1133, 1341
HhaI GCGC 3 cut(s) 66, 214, 1028
Hin1I GRCGYC 1 cut(s) 212
Hin6I GCGC 3 cut(s) 64, 212, 1026
HinP1I GCGC 3 cut(s) 64, 212, 1026
HinfI GANTC 4 cut(s) 296, 501, 1046, 1373
HpaII CCGG 4 cut(s) 261, 918, 1133, 1341
HphI GGTGA 3 cut(s) 790, 832, 1187
Hpy166II GTNNAC 6 cut(s) 79, 118, 276, 278, 352, 839
Hpy188I TCNGA 5 cut(s) 48, 331, 885, 1159, 1286
Hpy8I GTNNAC 6 cut(s) 79, 118, 276, 278, 352, 839
HpyAV CCTTC 7 cut(s) 370, 431, 486, 1244, 1416, 1465, 1582
HpyCH4III ACNGT 6 cut(s) 847, 948, 1021, 1201, 1552, 1662
HpyCH4IV ACGT 2 cut(s) 285, 289
HpyF10VI GCNNNNNNNGC 4 cut(s) 209, 380, 743, 1387
HpyF3I CTNAG 5 cut(s) 67, 505, 810, 1607, 1620
HpySE526I ACGT 2 cut(s) 285, 289
Hsp92I GRCGYC 1 cut(s) 212
HspAI GCGC 3 cut(s) 64, 212, 1026
KasI GGCGCC 1 cut(s) 211
Ksp22I TGATCA 1 cut(s) 445
Kzo9I GATC 8 cut(s) 48, 445, 531, 880, 1129, 1222, 1315, 1519
LguI GCTCTTC 1 cut(s) 954
LmnI GCTCC 3 cut(s) 61, 380, 1546
Lsp1109I GCAGC 4 cut(s) 190, 730, 910, 1421
LweI GCATC 7 cut(s) 134, 547, 823, 1150, 1231, 1399, 1659
MaeI CTAG 3 cut(s) 425, 965, 1488
MaeII ACGT 2 cut(s) 285, 289
MaeIII GTNAC 7 cut(s) 778, 820, 976, 1080, 1135, 1298, 1524
MalI GATC 8 cut(s) 50, 447, 533, 882, 1131, 1224, 1317, 1521
MboI GATC 8 cut(s) 48, 445, 531, 880, 1129, 1222, 1315, 1519
MboII GAAGA 3 cut(s) 971, 1156, 1574
MfeI CAATTG 1 cut(s) 1362
MflI RGATCY 2 cut(s) 531, 1129
MhlI GDGCHC 1 cut(s) 841
MluCI AATT 9 cut(s) 180, 359, 432, 561, 637, 792, 1335, 1362, 1466
Mly113I GGCGCC 1 cut(s) 212
MlyI GAGTC 1 cut(s) 495
Mph1103I ATGCAT 2 cut(s) 988, 1246
MseI TTAA 6 cut(s) 282, 317, 431, 851, 1125, 1506
MslI CAYNNNNRTG 8 cut(s) 246, 624, 714, 831, 987, 1202, 1293, 1395
MspI CCGG 4 cut(s) 261, 918, 1133, 1341
MspR9I CCNGG 5 cut(s) 262, 897, 919, 941, 1014
MunI CAATTG 1 cut(s) 1362
Mva1269I GAATGC 1 cut(s) 325
MvaI CCWGG 3 cut(s) 897, 941, 1014
MwoI GCNNNNNNNGC 4 cut(s) 209, 380, 743, 1387
NarI GGCGCC 1 cut(s) 212
NciI CCSGG 2 cut(s) 262, 919
NcoI CCATGG 2 cut(s) 1170, 1626
NdeI CATATG 1 cut(s) 988
NdeII GATC 8 cut(s) 48, 445, 531, 880, 1129, 1222, 1315, 1519
NlaIV GGNNCC 5 cut(s) 213, 409, 417, 725, 1542
NmuCI GTSAC 4 cut(s) 778, 820, 1080, 1135
NsiI ATGCAT 2 cut(s) 988, 1246
NspI RCATGY 1 cut(s) 986
NspV TTCGAA 1 cut(s) 1560
OliI CACNNNNGTG 1 cut(s) 831
PaeR7I CTCGAG 2 cut(s) 186, 1056
PagI TCATGA 2 cut(s) 292, 912
PceI AGGCCT 1 cut(s) 1426
PciSI GCTCTTC 1 cut(s) 954
PctI GAATGC 1 cut(s) 325
PfeI GAWTC 3 cut(s) 296, 1046, 1373
Pfl23II CGTACG 1 cut(s) 286
PflFI GACNNNGTC 1 cut(s) 113
PflMI CCANNNNNTGG 1 cut(s) 1537
PkrI GCNGC 4 cut(s) 205, 745, 925, 1411
PleI GAGTC 1 cut(s) 495
PluTI GGCGCC 1 cut(s) 215
PpsI GAGTC 1 cut(s) 495
PpuMI RGGWCCY 1 cut(s) 415
PshBI ATTAAT 1 cut(s) 317
Psp5II RGGWCCY 1 cut(s) 415
Psp6I CCWGG 3 cut(s) 895, 939, 1012
PspEI GGTNACC 2 cut(s) 820, 1524
PspGI CCWGG 3 cut(s) 895, 939, 1012
PspLI CGTACG 1 cut(s) 286
PspN4I GGNNCC 5 cut(s) 213, 409, 417, 725, 1542
PspPI GGNCC 3 cut(s) 238, 415, 1010
PspPPI RGGWCCY 1 cut(s) 415
PstNI CAGNNNCTG 1 cut(s) 946
PsuI RGATCY 2 cut(s) 531, 1129
PsyI GACNNNGTC 1 cut(s) 113
RsaI GTAC 4 cut(s) 277, 288, 945, 1605
RsaNI GTAC 4 cut(s) 276, 287, 944, 1604
RseI CAYNNNNRTG 8 cut(s) 246, 624, 714, 831, 987, 1202, 1293, 1395
SapI GCTCTTC 1 cut(s) 954
SaqAI TTAA 6 cut(s) 282, 317, 431, 851, 1125, 1506
SatI GCNGC 4 cut(s) 204, 744, 924, 1410
Sau3AI GATC 8 cut(s) 48, 445, 531, 880, 1129, 1222, 1315, 1519
Sau96I GGNCC 3 cut(s) 238, 415, 1010
SchI GAGTC 1 cut(s) 495
ScrFI CCNGG 5 cut(s) 262, 897, 919, 941, 1014
SduI GDGCHC 1 cut(s) 841
SfaNI GCATC 7 cut(s) 134, 547, 823, 1150, 1231, 1399, 1659
SfcI CTRYAG 3 cut(s) 79, 738, 1513
SfoI GGCGCC 1 cut(s) 213
Sfr274I CTCGAG 2 cut(s) 186, 1056
SfuI TTCGAA 1 cut(s) 1560
SinI GGWCC 3 cut(s) 238, 415, 1010
SlaI CTCGAG 2 cut(s) 186, 1056
SmiMI CAYNNNNRTG 8 cut(s) 246, 624, 714, 831, 987, 1202, 1293, 1395
SmlI CTYRAG 4 cut(s) 186, 747, 1056, 1403
SmoI CTYRAG 4 cut(s) 186, 747, 1056, 1403
Sse9I AATT 9 cut(s) 180, 359, 432, 561, 637, 792, 1335, 1362, 1466
SseBI AGGCCT 1 cut(s) 1426
SsiI CCGC 2 cut(s) 24, 1280
SspDI GGCGCC 1 cut(s) 211
SspI AATATT 2 cut(s) 220, 1436
SspMI CTAG 3 cut(s) 425, 965, 1488
StuI AGGCCT 1 cut(s) 1426
StyD4I CCNGG 5 cut(s) 260, 895, 917, 939, 1012
StyI CCWWGG 4 cut(s) 411, 1170, 1323, 1626
TaaI ACNGT 6 cut(s) 847, 948, 1021, 1201, 1552, 1662
TaiI ACGT 2 cut(s) 288, 292
TaqI TCGA 4 cut(s) 187, 1057, 1546, 1560
TasI AATT 9 cut(s) 180, 359, 432, 561, 637, 792, 1335, 1362, 1466
TatI WGTACW 2 cut(s) 275, 1603
TfiI GAWTC 3 cut(s) 296, 1046, 1373
Tru1I TTAA 6 cut(s) 282, 317, 431, 851, 1125, 1506
Tru9I TTAA 6 cut(s) 282, 317, 431, 851, 1125, 1506
TscAI CASTG 5 cut(s) 213, 979, 1204, 1273, 1383
TseFI GTSAC 4 cut(s) 778, 820, 1080, 1135
TseI GCWGC 4 cut(s) 203, 743, 923, 1409
Tsp45I GTSAC 4 cut(s) 778, 820, 1080, 1135
TspDTI ATGAA 6 cut(s) 335, 698, 1077, 1157, 1220, 1446
TspRI CASTG 5 cut(s) 213, 979, 1204, 1273, 1383
Tth111I GACNNNGTC 1 cut(s) 113
Van91I CCANNNNNTGG 1 cut(s) 1537
VneI GTGCAC 1 cut(s) 837
VpaK11BI GGWCC 3 cut(s) 238, 415, 1010
VspI ATTAAT 1 cut(s) 317
XapI RAATTY 2 cut(s) 180, 792
XceI RCATGY 1 cut(s) 986
XcmI CCANNNNNNNNNTGG 1 cut(s) 549
XhoI CTCGAG 2 cut(s) 186, 1056
XmiI GTMKAC 3 cut(s) 78, 117, 351
XspI CTAG 3 cut(s) 425, 965, 1488
Zsp2I ATGCAT 2 cut(s) 988, 1246
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.