RchiOBHm_Chr6g0282061

Belongs to the disease resistance NB-LRR family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
6
OG0019430
Physical Location & Seq
Reverse (-)
45387156 .. 45392720
5565 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 552 bp
ATGCATGGTATGTTATCGGATCGAAATCCTTGTGAGAACTATATGAGAATTGATCTTGATGCAGACTCTCTTGTGGGTAGCAGAATCAATATGCTGTTAAAGAGAACCAACACTCTAGTGTTAAACATGTTTAGATTGAGGGACGCTCTCAATCTCTTAGATACAGAGTGTTGTGTAAACTTGAAGTATCTTGAGCTGAATCACTGCCATGCTCTGGAATATGTGATTGCATGCATTGTCCTTCCTGTATTGGAGTCATTGACGATCCAAGGTGCCGAAATGCTAAAGGAGATTTGTCATGGTGAGGATCTGCTTCCAGTGGGGTCACGCAAGCAACTAAGCAAGTTGTCTCTGTCAAATTTACCTTCACTGACAAATTTTTGGAAGATAGAATCTCAACCTGAATACCTGAGAAATCTAAGAAGTGTTGAAGTGTGTTCATGTGAAAGATTGAAGTCTCTGTTCCAATTGCCGGGTGCTAGTAATCTGAAGGAGCTTGAAACGGTTGACATACAAAACTGCAGGGACTGGAAGAGATTTTTATTGGCCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NB-ARC

Protein Analysis

183

Amino Acids

21.0

Weight (kDa)

6.1

Isoelectric Point (pI)

44.52

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRR_RPS2 PF23247 54 - 175 2.6e-19 Plant disease resistance protein RPS2-like, leucine-rich repeats
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0019430)

Species Orthologous Gene IDs
rosa_chinensis RchiOBHm_Chr6g0282061
rosa_laevigata RLG00000012916
rosa_multiflora Rmu_sc0001700.1_g000010 Rmu_sc0001700.1_g000017
rosa_rugosa Rorug03G0141700 Rorug06G0145100
rosa_samantha Rh6BG261400 Rh6CG260300

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 272
AccB7I CCANNNNNTGG 1 cut(s) 214
AclWI GGATC 3 cut(s) 27, 259, 315
AcsI RAATTY 2 cut(s) 358, 376
AcuI CTGAAG 1 cut(s) 509
AfiI CCNNNNNNNGG 2 cut(s) 214, 472
AflIII ACRYGT 1 cut(s) 126
AgsI TTSAA 4 cut(s) 184, 431, 454, 500
AleI CACNNNNGTG 1 cut(s) 116
AluBI AGCT 2 cut(s) 196, 496
AluI AGCT 2 cut(s) 196, 496
Alw26I GTCTC 2 cut(s) 354, 462
AlwI GGATC 3 cut(s) 27, 259, 315
AlwNI CAGNNNCTG 1 cut(s) 528
AoxI GGCC 1 cut(s) 546
ApoI RAATTY 2 cut(s) 358, 376
AsuC2I CCSGG 1 cut(s) 474
AsuHPI GGTGA 1 cut(s) 314
BanI GGYRCC 1 cut(s) 272
BcnI CCSGG 1 cut(s) 474
BcoDI GTCTC 2 cut(s) 354, 462
BfaI CTAG 3 cut(s) 116, 480, 550
BfmI CTRYAG 1 cut(s) 520
Bme1390I CCNGG 1 cut(s) 474
BmiI GGNNCC 1 cut(s) 274
BmrFI CCNGG 1 cut(s) 474
BmsI GCATC 1 cut(s) 49
BplI GAGNNNNNCTC 2 cut(s) 130, 162
BpuEI CTTGAG 1 cut(s) 212
BpuMI CCSGG 1 cut(s) 474
BsaBI GATNNNNATC 1 cut(s) 24
BsaJI CCNNGG 1 cut(s) 268
Bsc4I CCNNNNNNNGG 2 cut(s) 214, 472
Bse1I ACTGG 2 cut(s) 317, 533
Bse8I GATNNNNATC 1 cut(s) 24
BseDI CCNNGG 1 cut(s) 268
BseJI GATNNNNATC 1 cut(s) 24
BseLI CCNNNNNNNGG 2 cut(s) 214, 472
BseMII CTCAG 1 cut(s) 401
BseNI ACTGG 2 cut(s) 317, 533
BshFI GGCC 1 cut(s) 548
BshNI GGYRCC 1 cut(s) 272
BsiSI CCGG 1 cut(s) 473
BslFI GGGAC 2 cut(s) 155, 539
BslI CCNNNNNNNGG 2 cut(s) 214, 472
BsmAI GTCTC 2 cut(s) 354, 462
BsmFI GGGAC 2 cut(s) 155, 539
BsnI GGCC 1 cut(s) 548
Bsp143I GATC 4 cut(s) 19, 52, 264, 307
BspANI GGCC 1 cut(s) 548
BspCNI CTCAG 1 cut(s) 402
BspLI GGNNCC 1 cut(s) 274
BspMAI CTGCAG 1 cut(s) 524
BspPI GGATC 3 cut(s) 27, 259, 315
BspT107I GGYRCC 1 cut(s) 272
BsrI ACTGG 2 cut(s) 317, 533
BssECI CCNNGG 1 cut(s) 268
BssMI GATC 4 cut(s) 19, 52, 264, 307
BssT1I CCWWGG 1 cut(s) 268
Bst4CI ACNGT 1 cut(s) 505
Bst6I CTCTTC 1 cut(s) 527
BstC8I GCNNGC 2 cut(s) 232, 332
BstDEI CTNAG 4 cut(s) 157, 338, 410, 419
BstKTI GATC 4 cut(s) 22, 55, 267, 310
BstMAI GTCTC 2 cut(s) 354, 462
BstMBI GATC 4 cut(s) 19, 52, 264, 307
BstNSI RCATGY 2 cut(s) 130, 234
BstSCI CCNGG 1 cut(s) 472
BstSFI CTRYAG 1 cut(s) 520
BstX2I RGATCY 1 cut(s) 307
BstYI RGATCY 1 cut(s) 307
BsuRI GGCC 1 cut(s) 548
BtsI GCAGTG 1 cut(s) 202
BtsIMutI CAGTG 3 cut(s) 202, 324, 368
Cac8I GCNNGC 2 cut(s) 232, 332
CaiI CAGNNNCTG 1 cut(s) 528
CseI GACGC 1 cut(s) 152
CviAII CATG 6 cut(s) 5, 127, 209, 231, 299, 441
CviJI RGCY 3 cut(s) 196, 496, 548
CviKI_1 RGCY 3 cut(s) 196, 496, 548
DdeI CTNAG 4 cut(s) 157, 338, 410, 419
DpnI GATC 4 cut(s) 21, 54, 266, 309
DpnII GATC 4 cut(s) 19, 52, 264, 307
Eam1104I CTCTTC 1 cut(s) 527
EarI CTCTTC 1 cut(s) 527
Eco130I CCWWGG 1 cut(s) 268
Eco57I CTGAAG 1 cut(s) 509
EcoT14I CCWWGG 1 cut(s) 268
EcoT22I ATGCAT 2 cut(s) 6, 236
ErhI CCWWGG 1 cut(s) 268
FaeI CATG 6 cut(s) 8, 130, 212, 234, 302, 444
FaqI GGGAC 2 cut(s) 155, 539
FatI CATG 6 cut(s) 4, 126, 208, 230, 298, 440
FspBI CTAG 3 cut(s) 116, 480, 550
HaeIII GGCC 1 cut(s) 548
HapII CCGG 1 cut(s) 473
HgaI GACGC 1 cut(s) 152
Hin1II CATG 6 cut(s) 8, 130, 212, 234, 302, 444
HincII GTYRAC 1 cut(s) 508
HindII GTYRAC 1 cut(s) 508
HinfI GANTC 5 cut(s) 65, 84, 199, 254, 392
HpaII CCGG 1 cut(s) 473
HphI GGTGA 1 cut(s) 314
Hpy166II GTNNAC 2 cut(s) 178, 508
Hpy188I TCNGA 2 cut(s) 19, 489
Hpy188III TCNNGA 3 cut(s) 56, 191, 215
Hpy8I GTNNAC 2 cut(s) 178, 508
HpyAV CCTTC 3 cut(s) 251, 375, 484
HpyCH4III ACNGT 1 cut(s) 505
HpyCH4V TGCA 5 cut(s) 4, 62, 230, 234, 522
HpyF3I CTNAG 4 cut(s) 157, 338, 410, 419
Hsp92II CATG 6 cut(s) 8, 130, 212, 234, 302, 444
Kzo9I GATC 4 cut(s) 19, 52, 264, 307
LmnI GCTCC 1 cut(s) 493
LpnPI CCDG 8 cut(s) 200, 258, 330, 414, 422, 486, 508, 514
LweI GCATC 1 cut(s) 49
MaeI CTAG 3 cut(s) 116, 480, 550
MaeIII GTNAC 1 cut(s) 324
MalI GATC 4 cut(s) 21, 54, 266, 309
MboI GATC 4 cut(s) 19, 52, 264, 307
MboII GAAGA 2 cut(s) 397, 544
MfeI CAATTG 1 cut(s) 467
MflI RGATCY 1 cut(s) 307
MluCI AATT 4 cut(s) 48, 358, 376, 467
MlyI GAGTC 2 cut(s) 59, 263
MnlI CCTC 2 cut(s) 132, 298
Mph1103I ATGCAT 2 cut(s) 6, 236
MseI TTAA 2 cut(s) 98, 122
MslI CAYNNNNRTG 2 cut(s) 116, 207
MspI CCGG 1 cut(s) 473
MspR9I CCNGG 1 cut(s) 474
MunI CAATTG 1 cut(s) 467
NciI CCSGG 1 cut(s) 474
NdeII GATC 4 cut(s) 19, 52, 264, 307
NlaIII CATG 6 cut(s) 8, 130, 212, 234, 302, 444
NlaIV GGNNCC 1 cut(s) 274
NmuCI GTSAC 1 cut(s) 324
NsiI ATGCAT 2 cut(s) 6, 236
NspI RCATGY 2 cut(s) 130, 234
OliI CACNNNNGTG 1 cut(s) 116
PaeI GCATGC 1 cut(s) 234
PciI ACATGT 1 cut(s) 126
PfeI GAWTC 3 cut(s) 84, 199, 392
PflMI CCANNNNNTGG 1 cut(s) 214
PleI GAGTC 2 cut(s) 59, 262
PpsI GAGTC 2 cut(s) 59, 262
PscI ACATGT 1 cut(s) 126
PspN4I GGNNCC 1 cut(s) 274
PstI CTGCAG 1 cut(s) 524
PstNI CAGNNNCTG 1 cut(s) 528
PsuI RGATCY 1 cut(s) 307
RseI CAYNNNNRTG 2 cut(s) 116, 207
SaqAI TTAA 2 cut(s) 98, 122
Sau3AI GATC 4 cut(s) 19, 52, 264, 307
SchI GAGTC 2 cut(s) 59, 263
ScrFI CCNGG 1 cut(s) 474
SetI ASST 6 cut(s) 198, 274, 367, 403, 411, 498
SfaNI GCATC 1 cut(s) 49
SfcI CTRYAG 1 cut(s) 520
SmiMI CAYNNNNRTG 2 cut(s) 116, 207
SmlI CTYRAG 1 cut(s) 191
SmoI CTYRAG 1 cut(s) 191
SphI GCATGC 1 cut(s) 234
Sse9I AATT 4 cut(s) 48, 358, 376, 467
SspMI CTAG 3 cut(s) 116, 480, 550
StyD4I CCNGG 1 cut(s) 472
StyI CCWWGG 1 cut(s) 268
TaaI ACNGT 1 cut(s) 505
TaqI TCGA 1 cut(s) 22
TasI AATT 4 cut(s) 48, 358, 376, 467
TfiI GAWTC 3 cut(s) 84, 199, 392
Tru1I TTAA 2 cut(s) 98, 122
Tru9I TTAA 2 cut(s) 98, 122
TscAI CASTG 3 cut(s) 209, 324, 375
TseFI GTSAC 1 cut(s) 324
Tsp45I GTSAC 1 cut(s) 324
TspDTI ATGAA 1 cut(s) 429
TspRI CASTG 3 cut(s) 209, 324, 375
Van91I CCANNNNNTGG 1 cut(s) 214
XapI RAATTY 2 cut(s) 358, 376
XceI RCATGY 2 cut(s) 130, 234
XspI CTAG 3 cut(s) 116, 480, 550
Zsp2I ATGCAT 2 cut(s) 6, 236
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.