RchiOBHm_Chr6g0294081

Belongs to the Ca(2 ) cation antiporter (CaCA) (TC 2.A.19) family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
6
OG0007448
Physical Location & Seq
Forward (+)
56535709 .. 56545635
9927 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1452 bp
ATGCCTGGACTTTCCAGCATAATTGGGCTGGAAACATGTGGAAGCTACTTGATTTTTGAGAGCGAAACTGCCCTCTGGAGTGGCGTCCGGGCATTCTTATATGTTCTTGGTCTTGCTTACTGTTTCCTTGGATTATCATCTATTACTGTTCGTTTTTTCCGGTCCATGGAGAGTGTTGTGAGCCACACGCGGAAGGTGGTCTCTAGTGATCAAGTTAGTAGGCATGAGAAAGTGTGGAATTTCACAATCGCAGATATTAGCCTGCTGGCCTTTGGGACCAGCTTTCCTCAAATATCATTAGCCACTATCGATGCTATACGAAACATTGGAAGCCTGTATGCTGGAGGTTTGGGTCCTGGAACACTAGTTGGTTCTGCTGCATTCGACCTTTTCCCCATCCATGCTGTTTGTGTTGTAGTTCCTAAAGCTGGAGAGCTGAAAAAGATAGCTGATATAGGAGTCTGGCTGGTTGAGCTCTTTTGGTCTTTCTGGGCATATATTTGGTTATATATAATTCTAGAGGTGTGGACACCAAACGTGATTACACTATGGGAGGCCTTATTGACAGTACTACAATACGGGTTACTGCTGATACATGCTTATGCTCAAGACAAGCAGTGGCCCTACTTTTCTCTTCCTTTGCCAAGAAGTGAGAGACCAGAGGACTGGGTGCCAGCTAAGCCTGTTGCATGCAAGCTTGATAGTGGTCTCTGTGATGAGTATTCTGAAATACTTCATGTTGGCGAAGGTGAAAACAGAGGCGTTGTTGATATTTTCTCCATACATTCAGAGACAGGAAATGTTCCAGTGTATCAAAAAGTCTCAGGAATTGACGAAACTCCTGAATCCTCTAATTCCTCATTAGAGGATTTTCAAGAGAAAATGGTGTCAGAAGATCATCCAGTTTTTACACTTTGGAGGCAACAATTTGTAGACGCAGTCCAGCTCGAAAGCACAGAATCAAGAAAAGTGAGAAACTTCTATTTGCGAATGGCAAGAATTTTCTGGCAATTACTTCTTGCTCCCTGGAGACTATTATTTGCATTTGTTCCTCCTTATAACATTGCTCATGGCTGGGTTGCCTTCATTTGTTCCCTAATTTTCATCAGTGCGATAGCTTACATTGTAACACAACTCACTGATCTCATAAGTTGTGTCACAGTGAATATATATGTTGGCATCGGTTTTCCATGGCTGATTGACACTGCATACAATTATTTTGTATATAAAGAACCACTGCGGATTGAGAATGCAGCGGGTCTTAGCTTCTCATTGCTTGTATTTTTCGCAACCTCAGTAGGCTGCATTTCAGTTCTGGTAATTAGGCGTCTAACATTGGGTGCAGAGCTTGGAGGGCCAAAGCTTTGGGCATGGATTACCTTTGTGTTTTTCATGTTGCTTTGGATAATTTTTGTTGTGCTGTCTTCTCTCAAAGTTTCTGGCATCATATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003008 GO:0003674 GO:0005215 GO:0005432 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005739 GO:0005856 GO:0005874 GO:0005886 GO:0005887 GO:0006810 GO:0006811 GO:0006812 GO:0006814 GO:0006816 GO:0006873 GO:0006874 GO:0006875 GO:0007204 GO:0007275 GO:0007399 GO:0007417 GO:0007420 GO:0007584 GO:0007610 GO:0007611 GO:0007612 GO:0007613 GO:0008150 GO:0008324 GO:0009605 GO:0009987 GO:0009991 GO:0010033 GO:0010646 GO:0010647 GO:0014070 GO:0015075 GO:0015077 GO:0015081 GO:0015085 GO:0015291 GO:0015297 GO:0015298 GO:0015318 GO:0015368 GO:0015491 GO:0015630 GO:0015672 GO:0016020 GO:0016021 GO:0019725 GO:0021537 GO:0022804 GO:0022857 GO:0022890 GO:0023051 GO:0023056 GO:0030001 GO:0030003 GO:0030425 GO:0030900 GO:0031224 GO:0031226 GO:0031667 GO:0032501 GO:0032502 GO:0033273 GO:0033280 GO:0033993 GO:0034220 GO:0035725 GO:0036477 GO:0042221 GO:0042592 GO:0042995 GO:0043005 GO:0043025 GO:0043197 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0044297 GO:0044309 GO:0044422 GO:0044424 GO:0044425 GO:0044430 GO:0044444 GO:0044446 GO:0044456 GO:0044459 GO:0044463 GO:0044464 GO:0045202 GO:0046873 GO:0048167 GO:0048168 GO:0048172 GO:0048513 GO:0048518 GO:0048522 GO:0048731 GO:0048856 GO:0048878 GO:0050789 GO:0050794 GO:0050801 GO:0050804 GO:0050806 GO:0050877 GO:0050890 GO:0050896 GO:0051179 GO:0051234 GO:0051480 GO:0051641 GO:0051649 GO:0055065 GO:0055074 GO:0055080 GO:0055082 GO:0055085 GO:0060291 GO:0060322 GO:0060401 GO:0060402 GO:0065007 GO:0065008 GO:0070509 GO:0070588 GO:0070838 GO:0071944 GO:0072503 GO:0072507 GO:0072511 GO:0097447 GO:0097458 GO:0097553 GO:0098655 GO:0098657 GO:0098659 GO:0098660 GO:0098662 GO:0098703 GO:0098739 GO:0098771 GO:0098794 GO:0099080 GO:0099081 GO:0099177 GO:0099512 GO:0099513 GO:0099516 GO:0099587 GO:0120025 GO:0120038 GO:1901660 GO:1901700 GO:1902656 GO:1990034
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Na_Ca_ex

Protein Analysis

483

Amino Acids

54.29

Weight (kDa)

5.36

Isoelectric Point (pI)

39.33

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Na_Ca_ex PF01699 34 - 201 6e-15 Sodium/calcium exchanger protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1059
AccB1I GGYRCC 1 cut(s) 670
AccI GTMKAC 1 cut(s) 933
AccII CGCG 1 cut(s) 190
AciI CCGC 3 cut(s) 190, 1240, 1256
AcsI RAATTY 2 cut(s) 238, 999
AcyI GRCGYC 2 cut(s) 84, 1327
AfaI GTAC 1 cut(s) 570
AfiI CCNNNNNNNGG 2 cut(s) 166, 428
AflIII ACRYGT 1 cut(s) 35
AgsI TTSAA 1 cut(s) 875
AhlI ACTAGT 1 cut(s) 364
AjnI CCWGG 3 cut(s) 4, 355, 1025
Alw21I GWGCWC 1 cut(s) 477
Alw26I GTCTC 6 cut(s) 205, 649, 713, 785, 826, 1024
AoxI GGCC 4 cut(s) 267, 555, 620, 1355
ApeKI GCWGC 3 cut(s) 377, 1253, 1302
ApoI RAATTY 2 cut(s) 238, 999
Asp700I GAANNNNTTC 1 cut(s) 732
AspS9I GGNCC 5 cut(s) 162, 276, 353, 621, 1355
AsuC2I CCSGG 1 cut(s) 89
AsuHPI GGTGA 1 cut(s) 761
AvaII GGWCC 3 cut(s) 162, 276, 353
BanI GGYRCC 1 cut(s) 670
BanII GRGCYC 1 cut(s) 477
BbsI GAAGAC 1 cut(s) 1416
Bbv12I GWGCWC 1 cut(s) 477
BbvI GCAGC 3 cut(s) 364, 1265, 1289
BccI CCATC 1 cut(s) 404
BciT130I CCWGG 3 cut(s) 6, 357, 1027
BclI TGATCA 1 cut(s) 208
BcnI CCSGG 1 cut(s) 89
BcoDI GTCTC 6 cut(s) 205, 649, 713, 785, 826, 1024
BcuI ACTAGT 1 cut(s) 364
BfaI CTAG 3 cut(s) 204, 365, 518
BisI GCNGC 3 cut(s) 378, 1254, 1303
BlpI GCTNAGC 1 cut(s) 678
BlsI GCNGC 3 cut(s) 379, 1255, 1304
BmcAI AGTACT 1 cut(s) 570
Bme1390I CCNGG 4 cut(s) 6, 89, 357, 1027
Bme18I GGWCC 3 cut(s) 162, 276, 353
BmgT120I GGNCC 5 cut(s) 162, 276, 353, 621, 1355
BmiI GGNNCC 3 cut(s) 277, 354, 672
BmrFI CCNGG 4 cut(s) 6, 89, 357, 1027
BmrI ACTGGG 1 cut(s) 676
BmsI GCATC 2 cut(s) 301, 1188
BmuI ACTGGG 1 cut(s) 676
BpiI GAAGAC 1 cut(s) 1416
BpmI CTGGAG 4 cut(s) 97, 363, 450, 1048
Bpu1102I GCTNAGC 1 cut(s) 678
BpuEI CTTGAG 1 cut(s) 591
BpuMI CCSGG 1 cut(s) 89
Bsa29I ATCGAT 1 cut(s) 309
BsaBI GATNNNNATC 1 cut(s) 136
BsaHI GRCGYC 2 cut(s) 84, 1327
BsaI GGTCTC 3 cut(s) 205, 649, 713
BsaJI CCNNGG 4 cut(s) 127, 165, 1025, 1190
BsaWI WCCGGW 1 cut(s) 159
Bsc4I CCNNNNNNNGG 2 cut(s) 166, 428
Bse1I ACTGG 3 cut(s) 671, 806, 902
Bse3DI GCAATG 2 cut(s) 1062, 1271
Bse8I GATNNNNATC 1 cut(s) 136
BseBI CCWGG 3 cut(s) 6, 357, 1027
BseCI ATCGAT 1 cut(s) 309
BseDI CCNNGG 4 cut(s) 127, 165, 1025, 1190
BseGI GGATG 2 cut(s) 396, 898
BseJI GATNNNNATC 1 cut(s) 136
BseLI CCNNNNNNNGG 2 cut(s) 166, 428
BseMI GCAATG 2 cut(s) 1062, 1271
BseMII CTCAG 2 cut(s) 837, 1308
BseNI ACTGG 3 cut(s) 671, 806, 902
BseXI GCAGC 3 cut(s) 364, 1265, 1289
BseYI CCCAGC 1 cut(s) 1074
BsgI GTGCAG 1 cut(s) 1362
Bsh1236I CGCG 1 cut(s) 190
BshFI GGCC 4 cut(s) 269, 557, 622, 1357
BshNI GGYRCC 1 cut(s) 670
BshVI ATCGAT 1 cut(s) 309
BsiHKAI GWGCWC 1 cut(s) 477
BsiSI CCGG 2 cut(s) 88, 160
BslFI GGGAC 1 cut(s) 289
BslI CCNNNNNNNGG 2 cut(s) 166, 428
BsmAI GTCTC 6 cut(s) 205, 649, 713, 785, 826, 1024
BsmFI GGGAC 1 cut(s) 289
BsmI GAATGC 3 cut(s) 92, 380, 1255
BsnI GGCC 4 cut(s) 269, 557, 622, 1357
Bso31I GGTCTC 3 cut(s) 205, 649, 713
Bsp1286I GDGCHC 1 cut(s) 477
Bsp143I GATC 3 cut(s) 208, 895, 1141
Bsp1720I GCTNAGC 1 cut(s) 678
Bsp19I CCATGG 2 cut(s) 165, 1190
BspACI CCGC 3 cut(s) 190, 1240, 1256
BspANI GGCC 4 cut(s) 269, 557, 622, 1357
BspCNI CTCAG 2 cut(s) 836, 1307
BspDI ATCGAT 1 cut(s) 309
BspFNI CGCG 1 cut(s) 190
BspLI GGNNCC 3 cut(s) 277, 354, 672
BspT107I GGYRCC 1 cut(s) 670
BspTNI GGTCTC 3 cut(s) 205, 649, 713
BsrDI GCAATG 2 cut(s) 1062, 1271
BsrI ACTGG 3 cut(s) 671, 806, 902
BssECI CCNNGG 4 cut(s) 127, 165, 1025, 1190
BssMI GATC 3 cut(s) 208, 895, 1141
BssNI GRCGYC 2 cut(s) 84, 1327
BssT1I CCWWGG 3 cut(s) 127, 165, 1190
Bst2UI CCWGG 3 cut(s) 6, 357, 1027
Bst4CI ACNGT 4 cut(s) 122, 148, 568, 1162
Bst6I CTCTTC 1 cut(s) 639
BstACI GRCGYC 2 cut(s) 84, 1327
BstC8I GCNNGC 5 cut(s) 263, 267, 675, 691, 695
BstDEI CTNAG 4 cut(s) 678, 823, 1262, 1294
BstDSI CCRYGG 2 cut(s) 165, 1190
BstF5I GGATG 2 cut(s) 396, 898
BstFNI CGCG 1 cut(s) 190
BstKTI GATC 3 cut(s) 211, 898, 1144
BstMAI GTCTC 6 cut(s) 205, 649, 713, 785, 826, 1024
BstMBI GATC 3 cut(s) 208, 895, 1141
BstMWI GCNNNNNNNGC 3 cut(s) 472, 679, 1354
BstNI CCWGG 3 cut(s) 6, 357, 1027
BstNSI RCATGY 3 cut(s) 39, 599, 693
BstSCI CCNGG 4 cut(s) 4, 87, 355, 1025
BstUI CGCG 1 cut(s) 190
BstV1I GCAGC 3 cut(s) 364, 1265, 1289
BstV2I GAAGAC 1 cut(s) 1416
BstXI CCANNNNNNTGG 2 cut(s) 666, 1365
Bsu15I ATCGAT 1 cut(s) 309
BsuRI GGCC 4 cut(s) 269, 557, 622, 1357
BsuTUI ATCGAT 1 cut(s) 309
BtgI CCRYGG 2 cut(s) 165, 1190
BtsCI GGATG 2 cut(s) 396, 898
BtsI GCAGTG 3 cut(s) 623, 1203, 1235
BtsIMutI CAGTG 7 cut(s) 623, 813, 1114, 1137, 1167, 1203, 1235
Cac8I GCNNGC 5 cut(s) 263, 267, 675, 691, 695
Cfr13I GGNCC 5 cut(s) 162, 276, 353, 621, 1355
ClaI ATCGAT 1 cut(s) 309
CseI GACGC 3 cut(s) 73, 944, 1316
Csp6I GTAC 1 cut(s) 569
CviQI GTAC 1 cut(s) 569
DdeI CTNAG 4 cut(s) 678, 823, 1262, 1294
DpnI GATC 3 cut(s) 210, 897, 1143
DpnII GATC 3 cut(s) 208, 895, 1141
Eam1104I CTCTTC 1 cut(s) 639
EarI CTCTTC 1 cut(s) 639
Ecl136II GAGCTC 1 cut(s) 475
Eco130I CCWWGG 3 cut(s) 127, 165, 1190
Eco147I AGGCCT 1 cut(s) 557
Eco24I GRGCYC 1 cut(s) 477
Eco31I GGTCTC 3 cut(s) 205, 649, 713
Eco47I GGWCC 3 cut(s) 162, 276, 353
Eco53kI GAGCTC 1 cut(s) 475
EcoICRI GAGCTC 1 cut(s) 475
EcoO109I RGGNCCY 1 cut(s) 353
EcoRII CCWGG 3 cut(s) 4, 355, 1025
EcoT14I CCWWGG 3 cut(s) 127, 165, 1190
EcoT38I GRGCYC 1 cut(s) 477
ErhI CCWWGG 3 cut(s) 127, 165, 1190
FaqI GGGAC 1 cut(s) 289
FauI CCCGC 1 cut(s) 1249
FbaI TGATCA 1 cut(s) 208
FblI GTMKAC 1 cut(s) 933
Fnu4HI GCNGC 3 cut(s) 378, 1254, 1303
FokI GGATG 2 cut(s) 383, 885
FriOI GRGCYC 1 cut(s) 477
Fsp4HI GCNGC 3 cut(s) 378, 1254, 1303
FspBI CTAG 3 cut(s) 204, 365, 518
GluI GCNGC 3 cut(s) 378, 1254, 1303
GsaI CCCAGC 1 cut(s) 1078
GsuI CTGGAG 4 cut(s) 97, 363, 450, 1048
HaeIII GGCC 4 cut(s) 269, 557, 622, 1357
HapII CCGG 2 cut(s) 88, 160
HgaI GACGC 3 cut(s) 73, 944, 1316
Hin1I GRCGYC 2 cut(s) 84, 1327
HindIII AAGCTT 2 cut(s) 695, 1361
HinfI GANTC 3 cut(s) 459, 845, 959
HpaII CCGG 2 cut(s) 88, 160
HphI GGTGA 1 cut(s) 761
Hpy166II GTNNAC 2 cut(s) 528, 934
Hpy188I TCNGA 3 cut(s) 727, 790, 892
Hpy188III TCNNGA 7 cut(s) 76, 518, 608, 825, 842, 875, 963
Hpy8I GTNNAC 2 cut(s) 528, 934
HpyAV CCTTC 3 cut(s) 187, 740, 1093
HpyCH4III ACNGT 4 cut(s) 122, 148, 568, 1162
HpyCH4IV ACGT 1 cut(s) 537
HpyCH4V TGCA 8 cut(s) 380, 689, 693, 1043, 1208, 1253, 1305, 1343
HpyF10VI GCNNNNNNNGC 3 cut(s) 472, 679, 1354
HpyF3I CTNAG 4 cut(s) 678, 823, 1262, 1294
HpySE526I ACGT 1 cut(s) 537
Hsp92I GRCGYC 2 cut(s) 84, 1327
Ksp22I TGATCA 1 cut(s) 208
Kzo9I GATC 3 cut(s) 208, 895, 1141
LmnI GCTCC 1 cut(s) 1027
Lsp1109I GCAGC 3 cut(s) 364, 1265, 1289
LweI GCATC 2 cut(s) 301, 1188
MaeI CTAG 3 cut(s) 204, 365, 518
MaeII ACGT 1 cut(s) 537
MaeIII GTNAC 3 cut(s) 582, 1126, 1156
MalI GATC 3 cut(s) 210, 897, 1143
MboI GATC 3 cut(s) 208, 895, 1141
MboII GAAGA 3 cut(s) 626, 905, 1416
MhlI GDGCHC 1 cut(s) 477
MlyI GAGTC 1 cut(s) 468
MroXI GAANNNNTTC 1 cut(s) 732
MslI CAYNNNNRTG 1 cut(s) 600
MspA1I CMGCKG 1 cut(s) 1256
MspI CCGG 2 cut(s) 88, 160
MspR9I CCNGG 4 cut(s) 6, 89, 357, 1027
Mva1269I GAATGC 3 cut(s) 92, 380, 1255
MvaI CCWGG 3 cut(s) 6, 357, 1027
MvnI CGCG 1 cut(s) 190
MwoI GCNNNNNNNGC 3 cut(s) 472, 679, 1354
NciI CCSGG 1 cut(s) 89
NcoI CCATGG 2 cut(s) 165, 1190
NdeII GATC 3 cut(s) 208, 895, 1141
NlaIV GGNNCC 3 cut(s) 277, 354, 672
NmuCI GTSAC 1 cut(s) 1156
NspI RCATGY 3 cut(s) 39, 599, 693
PaeI GCATGC 1 cut(s) 693
PceI AGGCCT 1 cut(s) 557
PciI ACATGT 1 cut(s) 35
PctI GAATGC 3 cut(s) 92, 380, 1255
PdmI GAANNNNTTC 1 cut(s) 732
PfeI GAWTC 2 cut(s) 845, 959
PflFI GACNNNGTC 1 cut(s) 938
PfoI TCCNGGA 1 cut(s) 355
PkrI GCNGC 3 cut(s) 379, 1255, 1304
PleI GAGTC 1 cut(s) 467
PpsI GAGTC 1 cut(s) 467
PpuMI RGGWCCY 1 cut(s) 353
PscI ACATGT 1 cut(s) 35
PsiI TTATAA 1 cut(s) 1059
Psp124BI GAGCTC 1 cut(s) 477
Psp5II RGGWCCY 1 cut(s) 353
Psp6I CCWGG 3 cut(s) 4, 355, 1025
PspFI CCCAGC 1 cut(s) 1074
PspGI CCWGG 3 cut(s) 4, 355, 1025
PspN4I GGNNCC 3 cut(s) 277, 354, 672
PspPI GGNCC 5 cut(s) 162, 276, 353, 621, 1355
PspPPI RGGWCCY 1 cut(s) 353
PsyI GACNNNGTC 1 cut(s) 938
RsaI GTAC 1 cut(s) 570
RsaNI GTAC 1 cut(s) 569
RseI CAYNNNNRTG 1 cut(s) 600
SacI GAGCTC 1 cut(s) 477
SatI GCNGC 3 cut(s) 378, 1254, 1303
Sau3AI GATC 3 cut(s) 208, 895, 1141
Sau96I GGNCC 5 cut(s) 162, 276, 353, 621, 1355
ScaI AGTACT 1 cut(s) 570
SchI GAGTC 1 cut(s) 468
ScrFI CCNGG 4 cut(s) 6, 89, 357, 1027
SduI GDGCHC 1 cut(s) 477
SfaNI GCATC 2 cut(s) 301, 1188
SinI GGWCC 3 cut(s) 162, 276, 353
SmiMI CAYNNNNRTG 1 cut(s) 600
SmlI CTYRAG 1 cut(s) 606
SmoI CTYRAG 1 cut(s) 606
SpeI ACTAGT 1 cut(s) 364
SphI GCATGC 1 cut(s) 693
SseBI AGGCCT 1 cut(s) 557
SsiI CCGC 3 cut(s) 190, 1240, 1256
SspMI CTAG 3 cut(s) 204, 365, 518
SstI GAGCTC 1 cut(s) 477
StuI AGGCCT 1 cut(s) 557
StyD4I CCNGG 4 cut(s) 4, 87, 355, 1025
StyI CCWWGG 3 cut(s) 127, 165, 1190
TaaI ACNGT 4 cut(s) 122, 148, 568, 1162
TaiI ACGT 1 cut(s) 540
TaqI TCGA 3 cut(s) 309, 384, 948
TatI WGTACW 1 cut(s) 568
TfiI GAWTC 2 cut(s) 845, 959
TscAI CASTG 7 cut(s) 623, 813, 1114, 1144, 1167, 1210, 1242
TseFI GTSAC 1 cut(s) 1156
TseI GCWGC 3 cut(s) 377, 1253, 1302
Tsp45I GTSAC 1 cut(s) 1156
TspDTI ATGAA 4 cut(s) 725, 1075, 1093, 1381
TspRI CASTG 7 cut(s) 623, 813, 1114, 1144, 1167, 1210, 1242
Tth111I GACNNNGTC 1 cut(s) 938
VpaK11BI GGWCC 3 cut(s) 162, 276, 353
XapI RAATTY 2 cut(s) 238, 999
XbaI TCTAGA 1 cut(s) 517
XceI RCATGY 3 cut(s) 39, 599, 693
XmiI GTMKAC 1 cut(s) 933
XmnI GAANNNNTTC 1 cut(s) 732
XspI CTAG 3 cut(s) 204, 365, 518
ZrmI AGTACT 1 cut(s) 570
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.