RchiOBHm_Chr6g0310191

1-aminocyclopropane-1-carboxylate synthase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
6
OG0014996
Physical Location & Seq
Forward (+)
67600830 .. 67602973
2144 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1380 bp
ATGTTGTCAAAAAAAGCTAGCTGCGACTCTCATGGCCAAGACTCATCCTACTTCCTGGGGTGGGAAAAATACGAGAAGAACCCTTACCATCAAGTTCATAATCCCACCGGAATCATCCAGATGGGACTTGCAGAGAATCAGCTTTCGTTTGACCTAGTTGAGTCATGGCTTGCAAGCAACCCAGATGCGGCGGGGCTCAAAAGAAACGGAGAATCCATCTTCCGAGAGCTAGCTCTCTTTCAAGACTATCACGGCTTGCCGGCTTTCAAGAAAGTATTGGTAGAAATGATGGCAAAAATGAGAGGCAACAAAGTGAAGTTGGAGGCAAAAAACCTGGTACTCACCGCCGGTGCCACCTCTGCCAATGAGACTCTTATGTTTTGCCTCGCCGATCCTGGCGAAGCCTTCCTCCTTCCCACTCCATACTATCCGGGATTTGATAGAGATCTCAAATGGCGTACCGGAGCCGAGATTGTTCCGATACAATGCACAAGTACAAACGGCTTCAAAATCACTGCCTCCGCACTAGAAGAAGCATACGAACGTGCCCAAAAATTCGACCTAAAAGTCAAGGGGGTATTGATCACCAATCCTTCAAACCCTCTCGGCACCGCCATGACTGTGGCTGAGCTCAACCACATCATCGACTTCGTCGTCGAAAAAGACATTCACATAATAAGCGACGAGATATATTCAGGCACCGTATTCAACTCTCCAAGCTTCGTGAGCATTCTAGAAGCTCTGATGCAGCGAAACCTAGAAAACACTGAACTGTGGAACCGTGTCCACATTGTTTACTCTCTCTCAAAGGACCTCGGCCTCCCCGGATTCCGAGTTGGCATGATCTACTCCAACAATACTGCGGTTGTTTCCGCCGCAACTAAAATGTCGAGCTTTGGTCTCATCTCTTCTCAAACTCAGTACTTACTATCCCAAATGTTGTCGGACAAGAAATTTACAAGAAAATACATGAGCGAGAACAAGAAGAGGCTCCTTAAGAGGAAGGAAATGCTCGTTTCCGGGCTGAAAAGCTCCGGCATCTCGTGTTTGGAAAGCAATGCCGGGCTGTTCTGTTGGGTGGACATGAGGCACTTGTTGAGAGCTAACACTTTTGAAGCTGAGAAGGAGCTTTGGGAAAAAGTGGTTTGTGAAGTTGGGTTGAACATCTCACCCGGGTCCTCTTGCCACTGCACTGAACCGGGTTGGTTCAGAATGTGCTTTGCTAACATGTCTGAAGAAACCCTTAGGGTTGCAATGCACAGGATCAAGGTCAGGCTTGTTGATGTTCATCGCTCCCGGGCCGCCATGGTTAAACCGGCGAGGTTATCGACCAAGTGGGTTCTGCAACTAGCTTCAAACAACCGTCGACCAGACCGTTAA

Protein Analysis

459

Amino Acids

51.65

Weight (kDa)

8.88

Isoelectric Point (pI)

43.0

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Aminotran_1_2 PF00155 39 - 422 6.2e-103 Aminotransferase class I and II
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014996)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G08040
fragaria_vesca FvH4_2g32470 FvH4_3g04180
malus_domestica MD08G1076500.v1.1 MD15G1145400.v1.1
prunus_persica Prupe.1G417800_v2.0.a1
pyrus_communis pycom08g06340
rosa_chinensis RchiOBHm_Chr6g0310191
rosa_laevigata RLG00000010501
rosa_multiflora Rmu_sc0008163.1_g000012
rosa_roxburghii Rroxscaffold_7G00158760
rosa_rugosa Rorug06G0378100
rosa_samantha Rh6BG501800 Rh6CG506800 Rh6DG492900
rosa_wichuraiana Rw6G042830

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 2 cut(s) 566, 653
AccB1I GGYRCC 3 cut(s) 350, 608, 698
AccI GTMKAC 1 cut(s) 1366
AciI CCGC 9 cut(s) 188, 191, 345, 522, 612, 863, 873, 876, 1302
AclWI GGATC 2 cut(s) 386, 1271
AcoI YGGCCR 1 cut(s) 34
AcsI RAATTY 2 cut(s) 554, 953
AcuI CTGAAG 1 cut(s) 1254
AfaI GTAC 4 cut(s) 339, 460, 496, 923
AfiI CCNNNNNNNGG 2 cut(s) 61, 187
AflII CTTAAG 1 cut(s) 995
AflIII ACRYGT 1 cut(s) 1227
AgsI TTSAA 8 cut(s) 242, 268, 508, 597, 709, 1115, 1162, 1356
AjnI CCWGG 3 cut(s) 54, 333, 394
Alw21I GWGCWC 1 cut(s) 633
Alw26I GTCTC 2 cut(s) 362, 905
AlwI GGATC 2 cut(s) 386, 1271
Ama87I CYCGRG 2 cut(s) 1172, 1296
AoxI GGCC 3 cut(s) 34, 817, 1299
ApeKI GCWGC 2 cut(s) 21, 748
ApoI RAATTY 2 cut(s) 554, 953
AspS9I GGNCC 3 cut(s) 811, 1176, 1299
AsuC2I CCSGG 9 cut(s) 432, 825, 1021, 1063, 1173, 1174, 1200, 1297, 1298
AsuHPI GGTGA 3 cut(s) 334, 577, 1161
AsuNHI GCTAGC 2 cut(s) 17, 229
AvaI CYCGRG 2 cut(s) 1172, 1296
AvaII GGWCC 2 cut(s) 811, 1176
AxyI CCTNAGG 1 cut(s) 1244
BaeGI GKGCMC 1 cut(s) 550
BalI TGGCCA 1 cut(s) 36
BanI GGYRCC 3 cut(s) 350, 608, 698
BanII GRGCYC 2 cut(s) 198, 633
BarI GAAGNNNNNNTAC 2 cut(s) 68, 100
BauI CACGAG 1 cut(s) 1042
Bbv12I GWGCWC 1 cut(s) 633
BbvI GCAGC 2 cut(s) 8, 760
BccI CCATC 4 cut(s) 96, 115, 224, 283
BceAI ACGGC 2 cut(s) 268, 517
BciT130I CCWGG 3 cut(s) 56, 335, 396
BclI TGATCA 1 cut(s) 582
BcnI CCSGG 9 cut(s) 432, 825, 1021, 1063, 1173, 1174, 1200, 1297, 1298
BcoDI GTCTC 2 cut(s) 362, 905
BfaI CTAG 7 cut(s) 18, 155, 230, 527, 734, 758, 1349
BfrI CTTAAG 1 cut(s) 995
BglII AGATCT 1 cut(s) 445
BisI GCNGC 5 cut(s) 22, 189, 749, 876, 1302
BlpI GCTNAGC 1 cut(s) 627
BlsI GCNGC 5 cut(s) 23, 190, 750, 877, 1303
BmcAI AGTACT 1 cut(s) 923
Bme18I GGWCC 2 cut(s) 811, 1176
BmeT110I CYCGRG 2 cut(s) 1172, 1296
BmgT120I GGNCC 3 cut(s) 811, 1176, 1299
BmiI GGNNCC 7 cut(s) 352, 466, 610, 700, 779, 992, 1177
BmsI GCATC 3 cut(s) 175, 735, 1047
BmtI GCTAGC 2 cut(s) 21, 233
Bpu1102I GCTNAGC 1 cut(s) 627
BpuMI CCSGG 9 cut(s) 432, 825, 1021, 1063, 1173, 1174, 1200, 1297, 1298
BsaBI GATNNNNATC 2 cut(s) 444, 1287
BsaI GGTCTC 1 cut(s) 905
BsaJI CCNNGG 6 cut(s) 55, 814, 823, 1172, 1296, 1305
BsaWI WCCGGW 2 cut(s) 107, 461
Bsc4I CCNNNNNNNGG 2 cut(s) 61, 187
Bse118I RCCGGY 3 cut(s) 259, 347, 1315
Bse21I CCTNAGG 1 cut(s) 1244
Bse3DI GCAATG 2 cut(s) 1063, 1260
Bse8I GATNNNNATC 2 cut(s) 444, 1287
BseBI CCWGG 3 cut(s) 56, 335, 396
BseDI CCNNGG 6 cut(s) 55, 814, 823, 1172, 1296, 1305
BseGI GGATG 2 cut(s) 44, 114
BseJI GATNNNNATC 2 cut(s) 444, 1287
BseLI CCNNNNNNNGG 2 cut(s) 61, 187
BseMI GCAATG 2 cut(s) 1063, 1260
BseMII CTCAG 3 cut(s) 618, 932, 1110
BseSI GKGCMC 1 cut(s) 550
BseXI GCAGC 2 cut(s) 8, 760
BsgI GTGCAG 1 cut(s) 1174
BshFI GGCC 3 cut(s) 36, 819, 1301
BshNI GGYRCC 3 cut(s) 350, 608, 698
BsiHKAI GWGCWC 1 cut(s) 633
BsiHKCI CYCGRG 2 cut(s) 1172, 1296
BslFI GGGAC 1 cut(s) 138
BslI CCNNNNNNNGG 2 cut(s) 61, 187
BsmAI GTCTC 2 cut(s) 362, 905
BsmFI GGGAC 1 cut(s) 138
BsmI GAATGC 1 cut(s) 729
BsnI GGCC 3 cut(s) 36, 819, 1301
Bso31I GGTCTC 1 cut(s) 905
BsoBI CYCGRG 2 cut(s) 1172, 1296
Bsp1286I GDGCHC 3 cut(s) 198, 550, 633
Bsp143I GATC 5 cut(s) 391, 445, 582, 843, 1263
Bsp1720I GCTNAGC 1 cut(s) 627
Bsp19I CCATGG 1 cut(s) 1305
BspACI CCGC 9 cut(s) 188, 191, 345, 522, 612, 863, 873, 876, 1302
BspANI GGCC 3 cut(s) 36, 819, 1301
BspCNI CTCAG 3 cut(s) 619, 931, 1111
BspLI GGNNCC 7 cut(s) 352, 466, 610, 700, 779, 992, 1177
BspOI GCTAGC 2 cut(s) 21, 233
BspPI GGATC 2 cut(s) 386, 1271
BspT107I GGYRCC 3 cut(s) 350, 608, 698
BspTI CTTAAG 1 cut(s) 995
BspTNI GGTCTC 1 cut(s) 905
BsrDI GCAATG 2 cut(s) 1063, 1260
BsrFI RCCGGY 3 cut(s) 259, 347, 1315
BssAI RCCGGY 3 cut(s) 259, 347, 1315
BssECI CCNNGG 6 cut(s) 55, 814, 823, 1172, 1296, 1305
BssMI GATC 5 cut(s) 391, 445, 582, 843, 1263
BssSI CACGAG 1 cut(s) 1042
BssT1I CCWWGG 1 cut(s) 1305
Bst2BI CACGAG 1 cut(s) 1042
Bst2UI CCWGG 3 cut(s) 56, 335, 396
Bst4CI ACNGT 6 cut(s) 622, 703, 774, 782, 1364, 1376
Bst6I CTCTTC 2 cut(s) 913, 980
BstAFI CTTAAG 1 cut(s) 995
BstC8I GCNNGC 6 cut(s) 19, 171, 175, 231, 257, 261
BstDEI CTNAG 4 cut(s) 627, 918, 1119, 1244
BstDSI CCRYGG 1 cut(s) 1305
BstF5I GGATG 2 cut(s) 44, 114
BstKTI GATC 5 cut(s) 394, 448, 585, 846, 1266
BstMAI GTCTC 2 cut(s) 362, 905
BstMBI GATC 5 cut(s) 391, 445, 582, 843, 1263
BstMWI GCNNNNNNNGC 2 cut(s) 359, 726
BstNI CCWGG 3 cut(s) 56, 335, 396
BstNSI RCATGY 1 cut(s) 1231
BstSLI GKGCMC 1 cut(s) 550
BstV1I GCAGC 2 cut(s) 8, 760
BstX2I RGATCY 1 cut(s) 445
BstXI CCANNNNNNTGG 1 cut(s) 622
BstYI RGATCY 1 cut(s) 445
Bsu36I CCTNAGG 1 cut(s) 1244
BsuRI GGCC 3 cut(s) 36, 819, 1301
BtgI CCRYGG 1 cut(s) 1305
BtgZI GCGATG 1 cut(s) 1274
BtsCI GGATG 2 cut(s) 44, 114
BtsI GCAGTG 2 cut(s) 513, 1186
BtsIMutI CAGTG 4 cut(s) 513, 765, 1186, 1191
Cac8I GCNNGC 6 cut(s) 19, 171, 175, 231, 257, 261
Cfr10I RCCGGY 3 cut(s) 259, 347, 1315
Cfr13I GGNCC 3 cut(s) 811, 1176, 1299
Cfr9I CCCGGG 2 cut(s) 1172, 1296
CsiI ACCWGGT 1 cut(s) 333
Csp6I GTAC 4 cut(s) 338, 459, 495, 922
CviAII CATG 8 cut(s) 32, 165, 616, 841, 970, 1084, 1228, 1306
CviQI GTAC 4 cut(s) 338, 459, 495, 922
DdeI CTNAG 4 cut(s) 627, 918, 1119, 1244
DpnI GATC 5 cut(s) 393, 447, 584, 845, 1265
DpnII GATC 5 cut(s) 391, 445, 582, 843, 1263
DrdI GACNNNNNNGTC 2 cut(s) 566, 653
DseDI GACNNNNNNGTC 2 cut(s) 566, 653
EaeI YGGCCR 1 cut(s) 34
Eam1104I CTCTTC 2 cut(s) 913, 980
EarI CTCTTC 2 cut(s) 913, 980
EciI GGCGGA 1 cut(s) 862
Ecl136II GAGCTC 1 cut(s) 631
Eco130I CCWWGG 1 cut(s) 1305
Eco24I GRGCYC 2 cut(s) 198, 633
Eco31I GGTCTC 1 cut(s) 905
Eco47I GGWCC 2 cut(s) 811, 1176
Eco53kI GAGCTC 1 cut(s) 631
Eco57I CTGAAG 1 cut(s) 1254
Eco81I CCTNAGG 1 cut(s) 1244
Eco88I CYCGRG 2 cut(s) 1172, 1296
EcoICRI GAGCTC 1 cut(s) 631
EcoO109I RGGNCCY 2 cut(s) 811, 1176
EcoRII CCWGG 3 cut(s) 54, 333, 394
EcoT14I CCWWGG 1 cut(s) 1305
EcoT38I GRGCYC 2 cut(s) 198, 633
ErhI CCWWGG 1 cut(s) 1305
FaeI CATG 8 cut(s) 35, 168, 619, 844, 973, 1087, 1231, 1309
FalI AAGNNNNNCTT 2 cut(s) 1227, 1259
FaqI GGGAC 1 cut(s) 138
FatI CATG 8 cut(s) 31, 164, 615, 840, 969, 1083, 1227, 1305
FauI CCCGC 1 cut(s) 184
FbaI TGATCA 1 cut(s) 582
FblI GTMKAC 1 cut(s) 1366
Fnu4HI GCNGC 5 cut(s) 22, 189, 749, 876, 1302
FokI GGATG 2 cut(s) 31, 101
FriOI GRGCYC 2 cut(s) 198, 633
Fsp4HI GCNGC 5 cut(s) 22, 189, 749, 876, 1302
FspBI CTAG 7 cut(s) 18, 155, 230, 527, 734, 758, 1349
GluI GCNGC 5 cut(s) 22, 189, 749, 876, 1302
HaeIII GGCC 3 cut(s) 36, 819, 1301
Hin1II CATG 8 cut(s) 35, 168, 619, 844, 973, 1087, 1231, 1309
HincII GTYRAC 1 cut(s) 1367
HindII GTYRAC 1 cut(s) 1367
HindIII AAGCTT 1 cut(s) 718
HinfI GANTC 8 cut(s) 26, 41, 111, 136, 161, 212, 370, 828
HphI GGTGA 3 cut(s) 334, 577, 1161
Hpy166II GTNNAC 4 cut(s) 787, 796, 1081, 1367
Hpy188I TCNGA 7 cut(s) 224, 480, 744, 833, 946, 1211, 1234
Hpy188III TCNNGA 5 cut(s) 118, 242, 268, 724, 734
Hpy8I GTNNAC 4 cut(s) 787, 796, 1081, 1367
Hpy99I CGWCG 4 cut(s) 656, 659, 686, 1368
HpyAV CCTTC 5 cut(s) 415, 422, 603, 997, 1117
HpyCH4III ACNGT 6 cut(s) 622, 703, 774, 782, 1364, 1376
HpyCH4IV ACGT 1 cut(s) 544
HpyCH4V TGCA 8 cut(s) 131, 173, 489, 748, 1191, 1253, 1258, 1345
HpyF10VI GCNNNNNNNGC 2 cut(s) 359, 726
HpyF3I CTNAG 4 cut(s) 627, 918, 1119, 1244
HpySE526I ACGT 1 cut(s) 544
Hsp92II CATG 8 cut(s) 35, 168, 619, 844, 973, 1087, 1231, 1309
KroI GCCGGC 1 cut(s) 259
KroNI GCCGGC 1 cut(s) 261
Ksp22I TGATCA 1 cut(s) 582
Kzo9I GATC 5 cut(s) 391, 445, 582, 843, 1263
LmnI GCTCC 5 cut(s) 464, 996, 1037, 1126, 1298
Lsp1109I GCAGC 2 cut(s) 8, 760
LweI GCATC 3 cut(s) 175, 735, 1047
MabI ACCWGGT 1 cut(s) 333
MaeI CTAG 7 cut(s) 18, 155, 230, 527, 734, 758, 1349
MaeII ACGT 1 cut(s) 544
MalI GATC 5 cut(s) 393, 447, 584, 845, 1265
MboI GATC 5 cut(s) 391, 445, 582, 843, 1263
MboII GAAGA 6 cut(s) 88, 211, 542, 900, 997, 1247
MflI RGATCY 1 cut(s) 445
MhlI GDGCHC 3 cut(s) 198, 550, 633
MlsI TGGCCA 1 cut(s) 36
MluCI AATT 2 cut(s) 554, 953
MluNI TGGCCA 1 cut(s) 36
MlyI GAGTC 4 cut(s) 20, 35, 170, 364
MmeI TCCRAC 3 cut(s) 300, 876, 924
Mox20I TGGCCA 1 cut(s) 36
MroNI GCCGGC 1 cut(s) 259
MscI TGGCCA 1 cut(s) 36
MseI TTAA 3 cut(s) 996, 1311, 1378
MslI CAYNNNNRTG 3 cut(s) 119, 614, 620
Msp20I TGGCCA 1 cut(s) 36
MspCI CTTAAG 1 cut(s) 995
Mva1269I GAATGC 1 cut(s) 729
MvaI CCWGG 3 cut(s) 56, 335, 396
MwoI GCNNNNNNNGC 2 cut(s) 359, 726
NaeI GCCGGC 1 cut(s) 261
NciI CCSGG 9 cut(s) 432, 825, 1021, 1063, 1173, 1174, 1200, 1297, 1298
NcoI CCATGG 1 cut(s) 1305
NdeII GATC 5 cut(s) 391, 445, 582, 843, 1263
NgoMIV GCCGGC 1 cut(s) 259
NheI GCTAGC 2 cut(s) 17, 229
NlaIII CATG 8 cut(s) 35, 168, 619, 844, 973, 1087, 1231, 1309
NlaIV GGNNCC 7 cut(s) 352, 466, 610, 700, 779, 992, 1177
NmeAIII GCCGAG 3 cut(s) 493, 585, 795
NspI RCATGY 1 cut(s) 1231
PciI ACATGT 1 cut(s) 1227
PcsI WCGNNNNNNNCGW 2 cut(s) 651, 1372
PctI GAATGC 1 cut(s) 729
PdiI GCCGGC 1 cut(s) 261
PfeI GAWTC 4 cut(s) 111, 136, 212, 828
PflFI GACNNNGTC 1 cut(s) 650
PfoI TCCNGGA 1 cut(s) 430
PkrI GCNGC 5 cut(s) 23, 190, 750, 877, 1303
PleI GAGTC 4 cut(s) 20, 35, 169, 364
PpsI GAGTC 4 cut(s) 20, 35, 169, 364
PpuMI RGGWCCY 2 cut(s) 811, 1176
PscI ACATGT 1 cut(s) 1227
Psp124BI GAGCTC 1 cut(s) 633
Psp5II RGGWCCY 2 cut(s) 811, 1176
Psp6I CCWGG 3 cut(s) 54, 333, 394
PspGI CCWGG 3 cut(s) 54, 333, 394
PspN4I GGNNCC 7 cut(s) 352, 466, 610, 700, 779, 992, 1177
PspPI GGNCC 3 cut(s) 811, 1176, 1299
PspPPI RGGWCCY 2 cut(s) 811, 1176
PsuI RGATCY 1 cut(s) 445
PsyI GACNNNGTC 1 cut(s) 650
RsaI GTAC 4 cut(s) 339, 460, 496, 923
RsaNI GTAC 4 cut(s) 338, 459, 495, 922
RseI CAYNNNNRTG 3 cut(s) 119, 614, 620
SacI GAGCTC 1 cut(s) 633
SalI GTCGAC 1 cut(s) 1365
SaqAI TTAA 3 cut(s) 996, 1311, 1378
SatI GCNGC 5 cut(s) 22, 189, 749, 876, 1302
Sau3AI GATC 5 cut(s) 391, 445, 582, 843, 1263
Sau96I GGNCC 3 cut(s) 811, 1176, 1299
ScaI AGTACT 1 cut(s) 923
SchI GAGTC 4 cut(s) 20, 35, 170, 364
SduI GDGCHC 3 cut(s) 198, 550, 633
SexAI ACCWGGT 1 cut(s) 333
SfaNI GCATC 3 cut(s) 175, 735, 1047
SgrAI CRCCGGYG 1 cut(s) 347
SinI GGWCC 2 cut(s) 811, 1176
SmaI CCCGGG 2 cut(s) 1174, 1298
SmiMI CAYNNNNRTG 3 cut(s) 119, 614, 620
SmlI CTYRAG 1 cut(s) 995
SmoI CTYRAG 1 cut(s) 995
Sse9I AATT 2 cut(s) 554, 953
SsiI CCGC 9 cut(s) 188, 191, 345, 522, 612, 863, 873, 876, 1302
SspMI CTAG 7 cut(s) 18, 155, 230, 527, 734, 758, 1349
SstI GAGCTC 1 cut(s) 633
StyI CCWWGG 1 cut(s) 1305
TaaI ACNGT 6 cut(s) 622, 703, 774, 782, 1364, 1376
TaiI ACGT 1 cut(s) 547
TaqI TCGA 6 cut(s) 558, 645, 657, 890, 1328, 1366
TasI AATT 2 cut(s) 554, 953
TatI WGTACW 2 cut(s) 494, 921
TauI GCSGC 3 cut(s) 191, 878, 1304
TfiI GAWTC 4 cut(s) 111, 136, 212, 828
Tru1I TTAA 3 cut(s) 996, 1311, 1378
Tru9I TTAA 3 cut(s) 996, 1311, 1378
TscAI CASTG 4 cut(s) 520, 772, 1193, 1198
TseI GCWGC 2 cut(s) 21, 748
TspDTI ATGAA 2 cut(s) 86, 1277
TspGWI ACGGA 1 cut(s) 222
TspMI CCCGGG 2 cut(s) 1172, 1296
TspRI CASTG 4 cut(s) 520, 772, 1193, 1198
Tth111I GACNNNGTC 1 cut(s) 650
Vha464I CTTAAG 1 cut(s) 995
VpaK11BI GGWCC 2 cut(s) 811, 1176
XapI RAATTY 2 cut(s) 554, 953
XbaI TCTAGA 1 cut(s) 733
XceI RCATGY 1 cut(s) 1231
XmaI CCCGGG 2 cut(s) 1172, 1296
XmiI GTMKAC 1 cut(s) 1366
XspI CTAG 7 cut(s) 18, 155, 230, 527, 734, 758, 1349
ZrmI AGTACT 1 cut(s) 923
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.