RchiOBHm_Chr5g0017231

NAD(P)H-quinone oxidoreductase chain 4

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
5
OG0020222
Physical Location & Seq
Forward (+)
11958899 .. 11960400
1502 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1425 bp
ATGAATAATTTTCCTTGGTTAACGCTAGTTGTAGTTTTGCCAATAACCGCGGGTTCCTTAATTTTCTTTCTTCCTCATAGAGGAAATAAGGTAATAAGATGGTATACTATATCTATATGCATAACGGAACTCCTTCTAACAACCTATGCATTCGGTTATCATTTCCAATTGGATGATCCGTTAATGCAACTAACGGAGAATTATCAATGGATCAATTTTTTTGATTTTTACTGGAGATTGGGAATAGATGGAATTTCTATAGGACCCATTTTACTGACAGGATTTATCACAACTTTAGCTACTTTAGCGGCTTGGCCCGTTACTCGAGATTCCCGACTATTCCATTTCCTAATAATTAATTCCCGTTTATCTACTTCTATCCATGTGGGGGGAAAGAAACGTTTGTATTCAGCTACAAAATTTATTTTGTACACTGCCGGAGGTTCCGTTTTTTTATTAATAGGAGTTCTGGGTATTGGTTTATATGGCTCCACTGAACCGACATTAAATTTTGAAACATCAGCTAATCAATCGTATCCTGTAGCCCTGGAAATAATATTCTATATTGGATTTCTTATTGCTTTTGCTGTCAAATCACCGATCATACCCCTACACACATGGTTACCAGACACCCATGGAGAGGCACATTATAGTACTTGTATGCTTTTAGCCGGAATCTTATTAAAAATGGGAGCGTATGGGTTGGTTCGGATCAATATGGAATTATTACCCCATGCCCATTCTATATTTTCTCCCTGGTTGATGATAGTGGGCACAATTCAAATTATCTATGCAGCTTTAACATCTCCTGGTCAGCGTAATTTAAAAAAAAGAATAGCCTATTCCTCTGTATCTCATATGGGTTTCATAATTATAGGAATTGGTTCTATAAGCGATACAGGGCTCAATGGGGCCATTTTACAAATAATTTCTCACGGATTTATTGGCGCTGCGCTTTTTTTCTTGGCCGGAACGAGTTATGATAGAATACGACTTATTTATCTCGACGAAATGGGCGGAATGGCTATCGCAATTCCAAAAATATTCACGACTTTCAGTATCTTATCAATGGCTTCGCTTGCATTACCGGGCATGAGCGGTTTTGTTGCCGAATTGATAGTTTTTTTTGGAATACTTACTAGCCAAAAATATCTTTTAATGACAAAAGTATTAATTACTTTTGTAATGGCAATTGGAATGATATTAACTCCTATTTATTCATTATCTATGTTACGCCAGATGTTCTATGGATACAAGCTTTTTAATGCCCCAAACTCTTATTTTTTTGATTCTGGACCGCGAGAGTTATTTATTTCTATTTCTATCCTTTTACCTGTAATAGGTATTGGTATTTATCCCGATTTCGTTTTCTCATTATCAGTTGACAAGGTCGAAGCTATTATATCAAATTTTTTTTATAGATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00190 ko01100 map00190 map01100
Pfam Domains
Protein Families
Proton_antipo_M

Protein Analysis

474

Amino Acids

52.79

Weight (kDa)

8.97

Isoelectric Point (pI)

29.2

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Proton_antipo_M PF00361 132 - 395 4.9e-52 NADH:quinone oxidoreductase/Mrp antiporter, TM
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0020222)

Species Orthologous Gene IDs
arabidopsis_thaliana ATCG01050
malus_domestica MD04G1189100.v1.1
rosa_chinensis RchiOBHm_Chr5g0017231 RchiOBHm_Chr5g0043051 RchiOBHm_CPg0502791
rosa_samantha Rh5BG295100

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 1098
AccI GTMKAC 1 cut(s) 104
AccII CGCG 2 cut(s) 50, 1300
AciI CCGC 6 cut(s) 48, 50, 308, 1017, 1098, 1298
AclI AACGTT 1 cut(s) 400
AclWI GGATC 3 cut(s) 170, 218, 719
AcoI YGGCCR 1 cut(s) 966
AcsI RAATTY 4 cut(s) 252, 419, 508, 1408
AfaI GTAC 2 cut(s) 431, 655
AfiI CCNNNNNNNGG 4 cut(s) 80, 388, 640, 1340
AgsI TTSAA 2 cut(s) 515, 782
AjnI CCWGG 3 cut(s) 546, 755, 808
AluBI AGCT 6 cut(s) 299, 413, 524, 797, 1258, 1397
AluI AGCT 6 cut(s) 299, 413, 524, 797, 1258, 1397
AlwI GGATC 3 cut(s) 170, 218, 719
Ama87I CYCGRG 1 cut(s) 324
AoxI GGCC 3 cut(s) 314, 912, 966
ApeKI GCWGC 2 cut(s) 794, 950
ApoI RAATTY 4 cut(s) 252, 419, 508, 1408
AseI ATTAAT 3 cut(s) 357, 458, 1172
Asp700I GAANNNNTTC 2 cut(s) 132, 883
AspLEI GCGC 2 cut(s) 950, 955
AspS9I GGNCC 4 cut(s) 263, 315, 912, 1295
AsuC2I CCSGG 1 cut(s) 1089
AsuHPI GGTGA 1 cut(s) 588
AvaI CYCGRG 1 cut(s) 324
AvaII GGWCC 2 cut(s) 263, 1295
BaeGI GKGCMC 1 cut(s) 776
BanII GRGCYC 1 cut(s) 906
BbvI GCAGC 2 cut(s) 806, 937
BccI CCATC 2 cut(s) 93, 242
BciT130I CCWGG 3 cut(s) 548, 757, 810
BciVI GTATCC 2 cut(s) 546, 1244
BcnI CCSGG 1 cut(s) 1089
BfaI CTAG 2 cut(s) 26, 1140
BfmI CTRYAG 2 cut(s) 258, 540
BfoI RGCGCY 1 cut(s) 951
BfuI GTATCC 2 cut(s) 546, 1244
BisI GCNGC 3 cut(s) 309, 795, 951
BlsI GCNGC 3 cut(s) 310, 796, 952
BmcAI AGTACT 1 cut(s) 655
Bme1390I CCNGG 4 cut(s) 548, 757, 810, 1089
Bme18I GGWCC 2 cut(s) 263, 1295
BmeT110I CYCGRG 1 cut(s) 324
BmgT120I GGNCC 4 cut(s) 263, 315, 912, 1295
BmiI GGNNCC 5 cut(s) 55, 265, 445, 490, 913
BmrFI CCNGG 4 cut(s) 548, 757, 810, 1089
BpmI CTGGAG 1 cut(s) 253
BpuMI CCSGG 1 cut(s) 1089
BsaJI CCNNGG 5 cut(s) 14, 48, 546, 634, 755
BsaXI ACNNNNNCTCC 2 cut(s) 432, 462
Bsc4I CCNNNNNNNGG 4 cut(s) 80, 388, 640, 1340
Bse1I ACTGG 1 cut(s) 236
BseBI CCWGG 3 cut(s) 548, 757, 810
BseDI CCNNGG 5 cut(s) 14, 48, 546, 634, 755
BseGI GGATG 1 cut(s) 178
BseLI CCNNNNNNNGG 4 cut(s) 80, 388, 640, 1340
BseNI ACTGG 1 cut(s) 236
BseSI GKGCMC 1 cut(s) 776
BseXI GCAGC 2 cut(s) 806, 937
Bsh1236I CGCG 2 cut(s) 50, 1300
BshFI GGCC 3 cut(s) 316, 914, 968
BsiHKCI CYCGRG 1 cut(s) 324
BsiSI CCGG 4 cut(s) 438, 672, 969, 1088
BslI CCNNNNNNNGG 4 cut(s) 80, 388, 640, 1340
BsmI GAATGC 1 cut(s) 149
BsnI GGCC 3 cut(s) 316, 914, 968
BsoBI CYCGRG 1 cut(s) 324
Bsp1286I GDGCHC 2 cut(s) 776, 906
Bsp1407I TGTACA 1 cut(s) 429
Bsp143I GATC 4 cut(s) 175, 210, 600, 711
Bsp19I CCATGG 1 cut(s) 634
BspACI CCGC 6 cut(s) 48, 50, 308, 1017, 1098, 1298
BspANI GGCC 3 cut(s) 316, 914, 968
BspFNI CGCG 2 cut(s) 50, 1300
BspLI GGNNCC 5 cut(s) 55, 265, 445, 490, 913
BspPI GGATC 3 cut(s) 170, 218, 719
BsrBI CCGCTC 1 cut(s) 1098
BsrGI TGTACA 1 cut(s) 429
BsrI ACTGG 1 cut(s) 236
BssECI CCNNGG 5 cut(s) 14, 48, 546, 634, 755
BssMI GATC 4 cut(s) 175, 210, 600, 711
BssNAI GTATAC 1 cut(s) 105
BssT1I CCWWGG 2 cut(s) 14, 634
Bst1107I GTATAC 1 cut(s) 105
Bst2UI CCWGG 3 cut(s) 548, 757, 810
BstAUI TGTACA 1 cut(s) 429
BstC8I GCNNGC 1 cut(s) 1080
BstDSI CCRYGG 2 cut(s) 48, 634
BstEII GGTNACC 1 cut(s) 621
BstENI CCTNNNNNAGG 2 cut(s) 78, 1338
BstF5I GGATG 1 cut(s) 178
BstFNI CGCG 2 cut(s) 50, 1300
BstH2I RGCGCY 1 cut(s) 951
BstHHI GCGC 2 cut(s) 950, 955
BstKTI GATC 4 cut(s) 178, 213, 603, 714
BstMBI GATC 4 cut(s) 175, 210, 600, 711
BstMWI GCNNNNNNNGC 2 cut(s) 305, 1079
BstNI CCWGG 3 cut(s) 548, 757, 810
BstPI GGTNACC 1 cut(s) 621
BstSCI CCNGG 4 cut(s) 546, 755, 808, 1087
BstSFI CTRYAG 2 cut(s) 258, 540
BstSLI GKGCMC 1 cut(s) 776
BstUI CGCG 2 cut(s) 50, 1300
BstV1I GCAGC 2 cut(s) 806, 937
BstZ17I GTATAC 1 cut(s) 105
BsuI GTATCC 2 cut(s) 546, 1244
BsuRI GGCC 3 cut(s) 316, 914, 968
BtgI CCRYGG 2 cut(s) 48, 634
BtsCI GGATG 1 cut(s) 178
BtsI GCAGTG 1 cut(s) 432
BtsIMutI CAGTG 2 cut(s) 432, 492
Cac8I GCNNGC 1 cut(s) 1080
CfoI GCGC 2 cut(s) 950, 955
Cfr13I GGNCC 4 cut(s) 263, 315, 912, 1295
Cfr42I CCGCGG 1 cut(s) 51
Csp6I GTAC 2 cut(s) 430, 654
CviAII CATG 5 cut(s) 383, 618, 635, 734, 1093
CviQI GTAC 2 cut(s) 430, 654
DpnI GATC 4 cut(s) 177, 212, 602, 713
DpnII GATC 4 cut(s) 175, 210, 600, 711
DraI TTTAAA 1 cut(s) 825
EaeI YGGCCR 1 cut(s) 966
EciI GGCGGA 1 cut(s) 1032
Eco130I CCWWGG 2 cut(s) 14, 634
Eco24I GRGCYC 1 cut(s) 906
Eco47I GGWCC 2 cut(s) 263, 1295
Eco88I CYCGRG 1 cut(s) 324
Eco91I GGTNACC 1 cut(s) 621
EcoNI CCTNNNNNAGG 2 cut(s) 78, 1338
EcoO109I RGGNCCY 1 cut(s) 263
EcoO65I GGTNACC 1 cut(s) 621
EcoRII CCWGG 3 cut(s) 546, 755, 808
EcoT14I CCWWGG 2 cut(s) 14, 634
EcoT22I ATGCAT 2 cut(s) 122, 151
EcoT38I GRGCYC 1 cut(s) 906
ErhI CCWWGG 2 cut(s) 14, 634
FaeI CATG 5 cut(s) 386, 621, 638, 737, 1096
FatI CATG 5 cut(s) 382, 617, 634, 733, 1092
FauI CCCGC 1 cut(s) 43
FauNDI CATATG 1 cut(s) 858
FblI GTMKAC 1 cut(s) 104
Fnu4HI GCNGC 3 cut(s) 309, 795, 951
FokI GGATG 1 cut(s) 185
FriOI GRGCYC 1 cut(s) 906
Fsp4HI GCNGC 3 cut(s) 309, 795, 951
FspBI CTAG 2 cut(s) 26, 1140
GlaI GCGC 2 cut(s) 949, 954
GluI GCNGC 3 cut(s) 309, 795, 951
GsuI CTGGAG 1 cut(s) 253
HaeII RGCGCY 1 cut(s) 951
HaeIII GGCC 3 cut(s) 316, 914, 968
HapII CCGG 4 cut(s) 438, 672, 969, 1088
HhaI GCGC 2 cut(s) 950, 955
Hin1II CATG 5 cut(s) 386, 621, 638, 737, 1096
Hin6I GCGC 2 cut(s) 948, 953
HinP1I GCGC 2 cut(s) 948, 953
HincII GTYRAC 2 cut(s) 21, 1384
HindII GTYRAC 2 cut(s) 21, 1384
HindIII AAGCTT 1 cut(s) 1256
HinfI GANTC 3 cut(s) 329, 675, 1289
HpaI GTTAAC 1 cut(s) 21
HpaII CCGG 4 cut(s) 438, 672, 969, 1088
HphI GGTGA 1 cut(s) 588
Hpy166II GTNNAC 4 cut(s) 21, 105, 432, 1384
Hpy188I TCNGA 1 cut(s) 711
Hpy188III TCNNGA 6 cut(s) 326, 333, 1004, 1048, 1293, 1358
Hpy8I GTNNAC 4 cut(s) 21, 105, 432, 1384
Hpy99I CGWCG 1 cut(s) 1010
HpyAV CCTTC 1 cut(s) 143
HpyCH4IV ACGT 1 cut(s) 400
HpyCH4V TGCA 5 cut(s) 120, 149, 187, 794, 1082
HpyF10VI GCNNNNNNNGC 2 cut(s) 305, 1079
HpySE526I ACGT 1 cut(s) 400
Hsp92II CATG 5 cut(s) 386, 621, 638, 737, 1096
HspAI GCGC 2 cut(s) 948, 953
KspAI GTTAAC 1 cut(s) 21
KspI CCGCGG 1 cut(s) 51
Kzo9I GATC 4 cut(s) 175, 210, 600, 711
LmnI GCTCC 2 cut(s) 494, 692
Lsp1109I GCAGC 2 cut(s) 806, 937
MaeI CTAG 2 cut(s) 26, 1140
MaeII ACGT 1 cut(s) 400
MaeIII GTNAC 3 cut(s) 319, 621, 1230
MalI GATC 4 cut(s) 177, 212, 602, 713
MbiI CCGCTC 1 cut(s) 1098
MboI GATC 4 cut(s) 175, 210, 600, 711
MboII GAAGA 1 cut(s) 62
MfeI CAATTG 2 cut(s) 167, 1191
MhlI GDGCHC 2 cut(s) 776, 906
MnlI CCTC 5 cut(s) 74, 84, 434, 634, 856
Mph1103I ATGCAT 2 cut(s) 122, 151
MroXI GAANNNNTTC 2 cut(s) 132, 883
MspA1I CMGCKG 1 cut(s) 50
MspI CCGG 4 cut(s) 438, 672, 969, 1088
MspR9I CCNGG 4 cut(s) 548, 757, 810, 1089
MteI GCGCNGCGC 1 cut(s) 951
MunI CAATTG 2 cut(s) 167, 1191
Mva1269I GAATGC 1 cut(s) 149
MvaI CCWGG 3 cut(s) 548, 757, 810
MvnI CGCG 2 cut(s) 50, 1300
MwoI GCNNNNNNNGC 2 cut(s) 305, 1079
NciI CCSGG 1 cut(s) 1089
NcoI CCATGG 1 cut(s) 634
NdeI CATATG 1 cut(s) 858
NdeII GATC 4 cut(s) 175, 210, 600, 711
NlaIII CATG 5 cut(s) 386, 621, 638, 737, 1096
NlaIV GGNNCC 5 cut(s) 55, 265, 445, 490, 913
NsiI ATGCAT 2 cut(s) 122, 151
PaeR7I CTCGAG 1 cut(s) 324
PcsI WCGNNNNNNNCGW 1 cut(s) 331
PctI GAATGC 1 cut(s) 149
PdmI GAANNNNTTC 2 cut(s) 132, 883
PfeI GAWTC 3 cut(s) 329, 675, 1289
PflFI GACNNNGTC 1 cut(s) 1388
PkrI GCNGC 3 cut(s) 310, 796, 952
PpuMI RGGWCCY 1 cut(s) 263
PshBI ATTAAT 3 cut(s) 357, 458, 1172
Psp1406I AACGTT 1 cut(s) 400
Psp5II RGGWCCY 1 cut(s) 263
Psp6I CCWGG 3 cut(s) 546, 755, 808
PspEI GGTNACC 1 cut(s) 621
PspGI CCWGG 3 cut(s) 546, 755, 808
PspN4I GGNNCC 5 cut(s) 55, 265, 445, 490, 913
PspPI GGNCC 4 cut(s) 263, 315, 912, 1295
PspPPI RGGWCCY 1 cut(s) 263
PsyI GACNNNGTC 1 cut(s) 1388
RsaI GTAC 2 cut(s) 431, 655
RsaNI GTAC 2 cut(s) 430, 654
SacII CCGCGG 1 cut(s) 51
SatI GCNGC 3 cut(s) 309, 795, 951
Sau3AI GATC 4 cut(s) 175, 210, 600, 711
Sau96I GGNCC 4 cut(s) 263, 315, 912, 1295
ScaI AGTACT 1 cut(s) 655
ScrFI CCNGG 4 cut(s) 548, 757, 810, 1089
SduI GDGCHC 2 cut(s) 776, 906
SfcI CTRYAG 2 cut(s) 258, 540
Sfr274I CTCGAG 1 cut(s) 324
Sfr303I CCGCGG 1 cut(s) 51
SgrBI CCGCGG 1 cut(s) 51
SinI GGWCC 2 cut(s) 263, 1295
SlaI CTCGAG 1 cut(s) 324
SmlI CTYRAG 1 cut(s) 324
SmoI CTYRAG 1 cut(s) 324
SsiI CCGC 6 cut(s) 48, 50, 308, 1017, 1098, 1298
SspI AATATT 2 cut(s) 558, 1044
SspMI CTAG 2 cut(s) 26, 1140
StyD4I CCNGG 4 cut(s) 546, 755, 808, 1087
StyI CCWWGG 2 cut(s) 14, 634
TaiI ACGT 1 cut(s) 403
TaqI TCGA 3 cut(s) 325, 1005, 1392
TatI WGTACW 2 cut(s) 429, 653
TauI GCSGC 1 cut(s) 311
TfiI GAWTC 3 cut(s) 329, 675, 1289
TscAI CASTG 2 cut(s) 439, 499
TseI GCWGC 2 cut(s) 794, 950
TspDTI ATGAA 3 cut(s) 17, 856, 1209
TspGWI ACGGA 5 cut(s) 140, 168, 209, 436, 951
TspRI CASTG 2 cut(s) 439, 499
Tth111I GACNNNGTC 1 cut(s) 1388
VpaK11BI GGWCC 2 cut(s) 263, 1295
VspI ATTAAT 3 cut(s) 357, 458, 1172
XagI CCTNNNNNAGG 2 cut(s) 78, 1338
XapI RAATTY 4 cut(s) 252, 419, 508, 1408
XcmI CCANNNNNNNNNTGG 1 cut(s) 1244
XhoI CTCGAG 1 cut(s) 324
XmiI GTMKAC 1 cut(s) 104
XmnI GAANNNNTTC 2 cut(s) 132, 883
XspI CTAG 2 cut(s) 26, 1140
ZrmI AGTACT 1 cut(s) 655
Zsp2I ATGCAT 2 cut(s) 122, 151
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.