RchiOBHm_Chr5g0017341

germin-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
5
OG0016285
Physical Location & Seq
Reverse (-)
12022590 .. 12023958
1369 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 645 bp
ATGGGCACTTATAATATCATCTTGGGGTTCCTGTTATCTTTCTCTTTGTCAGTTGCCACAGATCATAGTCCACTTCAAGATTTCTGTGTGGCAGACCCAGAAAGTCGAGTGCTGCTGAATGGCCTAGCTTGCAAGGACCCCAAGACAGTTAAAGCAAACGATTTTTCATTTGGTGGGCTTCACTTAGCAGCCAATACATCAAACCCAATTGGGTCAAGTGTGACACCGGTGACCGTATCCCAACTTCCAGGACTCAATACTCTTGGCATTTCACTAGTCCGCATCGACTATGCACCATCAGGCATCAACCCTCCCCACACTCATCCTCGTGCCTCCGAAATCTTGACCATCCTTGAAGGCTGCCTTGAAGTTGGGTTCGTAACGTCGAGCCCCGAAAATCGTCACATCACCAAGGTGTTGCAGAAGGGCGATGTGTTTGTTTTCCCAATCGGACTCGTCCACTACCAGAGAAATGTAGGAACTGGCAATGCTGTTGCTATTGCTGCCCTGAGCAGCCAAAATCCTGGTGTGGTAATAATTGCAAGTGCTGTCTTTGGGTCCAGGCCTAGTATTGGTAGTGAGATTCTCGTGAAGGCTTTGCAAGTTGATCAAGGGGTTGTCAATTACCTCCAGTCCATATTCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Cupin_1

Protein Analysis

214

Amino Acids

22.53

Weight (kDa)

6.27

Isoelectric Point (pI)

47.93

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Cupin_1 PF00190 63 - 205 7.6e-48 Cupin
Cupin_2 PF07883 95 - 167 5.3e-10 Cupin domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016285)

Species Orthologous Gene IDs
fragaria_vesca FvH4_3g10630 FvH4_3g10630
malus_domestica MD05G1269800.v1.1
prunus_persica Prupe.4G094700_v2.0.a1
pyrus_communis pycom05g25270
rosa_chinensis RchiOBHm_Chr5g0017341
rosa_laevigata RLG00000032275
rosa_multiflora Rmu_sc0005223.1_g000005 Rmu_sc0005319.1_g000008
rosa_roxburghii Rroxscaffold_1G00060020
rosa_rugosa Rorug05G0034100
rosa_samantha Rh5AG129200 Rh5BG127000 Rh5CG139800
rosa_wichuraiana Rw5G011160

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 12
AciI CCGC 1 cut(s) 280
AfiI CCNNNNNNNGG 1 cut(s) 572
AgeI ACCGGT 1 cut(s) 226
AgsI TTSAA 3 cut(s) 77, 356, 368
AhlI ACTAGT 1 cut(s) 274
AjnI CCWGG 3 cut(s) 247, 523, 560
AleI CACNNNNGTG 1 cut(s) 413
AluBI AGCT 1 cut(s) 128
AluI AGCT 1 cut(s) 128
AoxI GGCC 2 cut(s) 121, 563
ApeKI GCWGC 5 cut(s) 112, 188, 360, 503, 513
AsiGI ACCGGT 1 cut(s) 226
AspS9I GGNCC 2 cut(s) 136, 558
AsuHPI GGTGA 2 cut(s) 241, 400
AvaII GGWCC 2 cut(s) 136, 558
BaeGI GKGCMC 1 cut(s) 8
BanII GRGCYC 1 cut(s) 392
BarI GAAGNNNNNNTAC 2 cut(s) 228, 260
BauI CACGAG 2 cut(s) 327, 587
BbvI GCAGC 5 cut(s) 99, 200, 347, 490, 525
BccI CCATC 2 cut(s) 304, 356
BciT130I CCWGG 3 cut(s) 249, 525, 562
BciVI GTATCC 1 cut(s) 247
BclI TGATCA 1 cut(s) 607
BcuI ACTAGT 1 cut(s) 274
BfaI CTAG 3 cut(s) 125, 275, 567
BfuI GTATCC 1 cut(s) 247
BisI GCNGC 5 cut(s) 113, 189, 361, 504, 514
BlsI GCNGC 5 cut(s) 114, 190, 362, 505, 515
Bme1390I CCNGG 3 cut(s) 249, 525, 562
Bme18I GGWCC 2 cut(s) 136, 558
BmgT120I GGNCC 2 cut(s) 136, 558
BmiI GGNNCC 3 cut(s) 29, 138, 559
BmrFI CCNGG 3 cut(s) 249, 525, 562
BmsI GCATC 2 cut(s) 291, 312
BpmI CTGGAG 1 cut(s) 614
Bpu10I CCTNAGC 1 cut(s) 509
BsaJI CCNNGG 1 cut(s) 411
BsaWI WCCGGW 1 cut(s) 226
Bsc4I CCNNNNNNNGG 1 cut(s) 572
Bse118I RCCGGY 1 cut(s) 226
Bse1I ACTGG 2 cut(s) 487, 631
Bse3DI GCAATG 1 cut(s) 493
BseBI CCWGG 3 cut(s) 249, 525, 562
BseDI CCNNGG 1 cut(s) 411
BseGI GGATG 2 cut(s) 322, 348
BseLI CCNNNNNNNGG 1 cut(s) 572
BseMI GCAATG 1 cut(s) 493
BseMII CTCAG 1 cut(s) 500
BseNI ACTGG 2 cut(s) 487, 631
BseSI GKGCMC 1 cut(s) 8
BseXI GCAGC 5 cut(s) 99, 200, 347, 490, 525
BshFI GGCC 2 cut(s) 123, 565
BshTI ACCGGT 1 cut(s) 226
BsiSI CCGG 1 cut(s) 227
BslI CCNNNNNNNGG 1 cut(s) 572
BsnI GGCC 2 cut(s) 123, 565
Bsp1286I GDGCHC 2 cut(s) 8, 392
Bsp143I GATC 2 cut(s) 61, 607
BspACI CCGC 1 cut(s) 280
BspANI GGCC 2 cut(s) 123, 565
BspCNI CTCAG 1 cut(s) 501
BspLI GGNNCC 3 cut(s) 29, 138, 559
BsrDI GCAATG 1 cut(s) 493
BsrFI RCCGGY 1 cut(s) 226
BsrI ACTGG 2 cut(s) 487, 631
BssAI RCCGGY 1 cut(s) 226
BssECI CCNNGG 1 cut(s) 411
BssMI GATC 2 cut(s) 61, 607
BssSI CACGAG 2 cut(s) 327, 587
BssT1I CCWWGG 1 cut(s) 411
Bst2BI CACGAG 2 cut(s) 327, 587
Bst2UI CCWGG 3 cut(s) 249, 525, 562
Bst4CI ACNGT 2 cut(s) 148, 235
BstC8I GCNNGC 1 cut(s) 130
BstDEI CTNAG 2 cut(s) 184, 509
BstEII GGTNACC 1 cut(s) 229
BstF5I GGATG 2 cut(s) 322, 348
BstKTI GATC 2 cut(s) 64, 610
BstMBI GATC 2 cut(s) 61, 607
BstMWI GCNNNNNNNGC 2 cut(s) 129, 503
BstNI CCWGG 3 cut(s) 249, 525, 562
BstPI GGTNACC 1 cut(s) 229
BstSCI CCNGG 3 cut(s) 247, 523, 560
BstSLI GKGCMC 1 cut(s) 8
BstV1I GCAGC 5 cut(s) 99, 200, 347, 490, 525
BstXI CCANNNNNNTGG 1 cut(s) 524
BsuI GTATCC 1 cut(s) 247
BsuRI GGCC 2 cut(s) 123, 565
BtgZI GCGATG 1 cut(s) 444
BtsCI GGATG 2 cut(s) 322, 348
Cac8I GCNNGC 1 cut(s) 130
Cfr10I RCCGGY 1 cut(s) 226
Cfr13I GGNCC 2 cut(s) 136, 558
CspAI ACCGGT 1 cut(s) 226
CviJI RGCY 9 cut(s) 123, 128, 178, 191, 360, 390, 516, 565, 596
CviKI_1 RGCY 9 cut(s) 123, 128, 178, 191, 360, 390, 516, 565, 596
DdeI CTNAG 2 cut(s) 184, 509
DpnI GATC 2 cut(s) 63, 609
DpnII GATC 2 cut(s) 61, 607
Eco130I CCWWGG 1 cut(s) 411
Eco147I AGGCCT 1 cut(s) 565
Eco24I GRGCYC 1 cut(s) 392
Eco47I GGWCC 2 cut(s) 136, 558
Eco91I GGTNACC 1 cut(s) 229
EcoO109I RGGNCCY 1 cut(s) 136
EcoO65I GGTNACC 1 cut(s) 229
EcoRII CCWGG 3 cut(s) 247, 523, 560
EcoT14I CCWWGG 1 cut(s) 411
EcoT38I GRGCYC 1 cut(s) 392
ErhI CCWWGG 1 cut(s) 411
FaiI YATR 4 cut(s) 12, 66, 291, 638
FalI AAGNNNNNCTT 2 cut(s) 348, 380
FbaI TGATCA 1 cut(s) 607
Fnu4HI GCNGC 5 cut(s) 113, 189, 361, 504, 514
FokI GGATG 2 cut(s) 309, 335
FriOI GRGCYC 1 cut(s) 392
Fsp4HI GCNGC 5 cut(s) 113, 189, 361, 504, 514
FspBI CTAG 3 cut(s) 125, 275, 567
GluI GCNGC 5 cut(s) 113, 189, 361, 504, 514
GsuI CTGGAG 1 cut(s) 614
HaeIII GGCC 2 cut(s) 123, 565
HapII CCGG 1 cut(s) 227
HinfI GANTC 3 cut(s) 252, 453, 583
HpaII CCGG 1 cut(s) 227
HphI GGTGA 2 cut(s) 241, 400
Hpy166II GTNNAC 2 cut(s) 71, 460
Hpy188I TCNGA 2 cut(s) 337, 452
Hpy188III TCNNGA 3 cut(s) 77, 343, 589
Hpy8I GTNNAC 2 cut(s) 71, 460
Hpy99I CGWCG 1 cut(s) 388
HpyAV CCTTC 3 cut(s) 350, 418, 586
HpyCH4III ACNGT 2 cut(s) 148, 235
HpyCH4IV ACGT 1 cut(s) 383
HpyCH4V TGCA 5 cut(s) 132, 293, 421, 542, 601
HpyF10VI GCNNNNNNNGC 2 cut(s) 129, 503
HpyF3I CTNAG 2 cut(s) 184, 509
HpySE526I ACGT 1 cut(s) 383
Ksp22I TGATCA 1 cut(s) 607
Kzo9I GATC 2 cut(s) 61, 607
Lsp1109I GCAGC 5 cut(s) 99, 200, 347, 490, 525
LweI GCATC 2 cut(s) 291, 312
MaeI CTAG 3 cut(s) 125, 275, 567
MaeII ACGT 1 cut(s) 383
MaeIII GTNAC 4 cut(s) 220, 229, 379, 401
MalI GATC 2 cut(s) 63, 609
MboI GATC 2 cut(s) 61, 607
MfeI CAATTG 1 cut(s) 207
MhlI GDGCHC 2 cut(s) 8, 392
MluCI AATT 3 cut(s) 207, 537, 622
MlyI GAGTC 2 cut(s) 246, 447
MnlI CCTC 4 cut(s) 321, 336, 343, 638
MseI TTAA 1 cut(s) 150
MslI CAYNNNNRTG 2 cut(s) 327, 413
MspI CCGG 1 cut(s) 227
MspR9I CCNGG 3 cut(s) 249, 525, 562
MunI CAATTG 1 cut(s) 207
MvaI CCWGG 3 cut(s) 249, 525, 562
MwoI GCNNNNNNNGC 2 cut(s) 129, 503
NdeII GATC 2 cut(s) 61, 607
NlaIV GGNNCC 3 cut(s) 29, 138, 559
NmuCI GTSAC 3 cut(s) 220, 229, 401
OliI CACNNNNGTG 1 cut(s) 413
PceI AGGCCT 1 cut(s) 565
PfeI GAWTC 1 cut(s) 583
PfoI TCCNGGA 1 cut(s) 247
PinAI ACCGGT 1 cut(s) 226
PkrI GCNGC 5 cut(s) 114, 190, 362, 505, 515
PleI GAGTC 2 cut(s) 246, 447
PpsI GAGTC 2 cut(s) 246, 447
PpuMI RGGWCCY 1 cut(s) 136
PsiI TTATAA 1 cut(s) 12
Psp5II RGGWCCY 1 cut(s) 136
Psp6I CCWGG 3 cut(s) 247, 523, 560
PspEI GGTNACC 1 cut(s) 229
PspGI CCWGG 3 cut(s) 247, 523, 560
PspN4I GGNNCC 3 cut(s) 29, 138, 559
PspPI GGNCC 2 cut(s) 136, 558
PspPPI RGGWCCY 1 cut(s) 136
RseI CAYNNNNRTG 2 cut(s) 327, 413
SaqAI TTAA 1 cut(s) 150
SatI GCNGC 5 cut(s) 113, 189, 361, 504, 514
Sau3AI GATC 2 cut(s) 61, 607
Sau96I GGNCC 2 cut(s) 136, 558
SchI GAGTC 2 cut(s) 246, 447
ScrFI CCNGG 3 cut(s) 249, 525, 562
SduI GDGCHC 2 cut(s) 8, 392
SetI ASST 4 cut(s) 130, 386, 417, 630
SfaNI GCATC 2 cut(s) 291, 312
SgrAI CRCCGGYG 1 cut(s) 226
SinI GGWCC 2 cut(s) 136, 558
SmiMI CAYNNNNRTG 2 cut(s) 327, 413
SpeI ACTAGT 1 cut(s) 274
Sse9I AATT 3 cut(s) 207, 537, 622
SseBI AGGCCT 1 cut(s) 565
SsiI CCGC 1 cut(s) 280
SspMI CTAG 3 cut(s) 125, 275, 567
StuI AGGCCT 1 cut(s) 565
StyD4I CCNGG 3 cut(s) 247, 523, 560
StyI CCWWGG 1 cut(s) 411
TaaI ACNGT 2 cut(s) 148, 235
TaiI ACGT 1 cut(s) 386
TaqI TCGA 3 cut(s) 106, 285, 386
TasI AATT 3 cut(s) 207, 537, 622
TfiI GAWTC 1 cut(s) 583
Tru1I TTAA 1 cut(s) 150
Tru9I TTAA 1 cut(s) 150
TseFI GTSAC 3 cut(s) 220, 229, 401
TseI GCWGC 5 cut(s) 112, 188, 360, 503, 513
Tsp45I GTSAC 3 cut(s) 220, 229, 401
TspDTI ATGAA 1 cut(s) 156
VpaK11BI GGWCC 2 cut(s) 136, 558
XspI CTAG 3 cut(s) 125, 275, 567
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.