RchiOBHm_Chr5g0025461

Acetolactate synthase small subunit 1, chloroplastic-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
5
OG0018922
Physical Location & Seq
Forward (+)
19369246 .. 19370151
906 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 393 bp
ATGGGGGGAGCTCGTTCTAGGAAGAGAAACCAAAAAATAGAATCTATTCATGCCTTTTTGGATGATGGAAAATGGTGGTTGTTTCTCCAGGTTCGGGATATTACACACCTACCATTTGCTGAGCGAGAGTTGATGTTAATAAAGATTGCTGTAAACACTACTGCTAGAAGGGATGTTCTTGATATTGCTGGCATTTTCCGTGCTAAAGCCGTTGATGTTTCTGATCACACAATTACTCTTGAGCTTACTGGCGATTTGAATAAAATGATTGTACTGCAGAAGTTACTAGAGCCCTATGGGCTTTGCGAGATTGCACGAACTGGGAGAATGGCATTGGAGCGGGGCTCTCGTGTGGATTCCACTTATCTCCGTGGATATCCCCTTCCATTATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00290 ko00650 ko00660 ko00770 ko01100 ko01110 ko01130 ko01210 ko01230 map00290 map00650 map00660 map00770 map01100 map01110 map01130 map01210 map01230
Pfam Domains
Protein Families
ACT ACT_5 ALS_ss_C

Protein Analysis

130

Amino Acids

14.82

Weight (kDa)

9.46

Isoelectric Point (pI)

39.6

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ALS_ss_C PF10369 42 - 115 2.1e-26 Small subunit of acetolactate synthase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0018922)

Species Orthologous Gene IDs
pyrus_communis pycom02g00140
rosa_chinensis RchiOBHm_Chr5g0025461
rosa_multiflora Rmu_co8399419.1_g000001 Rmu_sc0000438.1_g000013 Rmu_sc0016852.1_g000007
rosa_samantha Rh5DG176900
rosa_wichuraiana Rw1G008670 Rw1G025300 Rw4G002960

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 391
AccBSI CCGCTC 1 cut(s) 340
AciI CCGC 1 cut(s) 340
AfaI GTAC 1 cut(s) 273
AfiI CCNNNNNNNGG 1 cut(s) 94
AgsI TTSAA 1 cut(s) 259
AjnI CCWGG 1 cut(s) 87
AluBI AGCT 2 cut(s) 11, 244
AluI AGCT 2 cut(s) 11, 244
Alw21I GWGCWC 1 cut(s) 13
Asp700I GAANNNNTTC 1 cut(s) 45
BanII GRGCYC 3 cut(s) 13, 294, 347
BauI CACGAG 1 cut(s) 348
Bbv12I GWGCWC 1 cut(s) 13
BccI CCATC 1 cut(s) 59
BceAI ACGGC 1 cut(s) 194
BciT130I CCWGG 1 cut(s) 89
BclI TGATCA 1 cut(s) 223
BfaI CTAG 3 cut(s) 18, 165, 287
BfmI CTRYAG 1 cut(s) 275
BglI GCCNNNNNGGC 1 cut(s) 298
BlpI GCTNAGC 1 cut(s) 120
Bme1390I CCNGG 1 cut(s) 89
BmrFI CCNGG 1 cut(s) 89
BmrI ACTGGG 1 cut(s) 330
BmuI ACTGGG 1 cut(s) 330
BplI GAGNNNNNCTC 2 cut(s) 329, 361
BpmI CTGGAG 1 cut(s) 71
Bpu1102I GCTNAGC 1 cut(s) 120
BpuEI CTTGAG 1 cut(s) 260
BsaJI CCNNGG 1 cut(s) 370
Bsc4I CCNNNNNNNGG 1 cut(s) 94
Bse1I ACTGG 2 cut(s) 253, 325
BseBI CCWGG 1 cut(s) 89
BseDI CCNNGG 1 cut(s) 370
BseGI GGATG 2 cut(s) 67, 178
BseLI CCNNNNNNNGG 1 cut(s) 94
BseMII CTCAG 1 cut(s) 111
BseNI ACTGG 2 cut(s) 253, 325
BsiHKAI GWGCWC 1 cut(s) 13
BslI CCNNNNNNNGG 1 cut(s) 94
Bsp1286I GDGCHC 3 cut(s) 13, 294, 347
Bsp143I GATC 1 cut(s) 223
Bsp1720I GCTNAGC 1 cut(s) 120
BspACI CCGC 1 cut(s) 340
BspCNI CTCAG 1 cut(s) 112
BspMAI CTGCAG 1 cut(s) 279
BsrBI CCGCTC 1 cut(s) 340
BsrI ACTGG 2 cut(s) 253, 325
BssECI CCNNGG 1 cut(s) 370
BssMI GATC 1 cut(s) 223
BssSI CACGAG 1 cut(s) 348
Bst2BI CACGAG 1 cut(s) 348
Bst2UI CCWGG 1 cut(s) 89
Bst6I CTCTTC 1 cut(s) 17
BstC8I GCNNGC 1 cut(s) 190
BstDEI CTNAG 1 cut(s) 120
BstDSI CCRYGG 1 cut(s) 370
BstF5I GGATG 2 cut(s) 67, 178
BstKTI GATC 1 cut(s) 226
BstMBI GATC 1 cut(s) 223
BstMWI GCNNNNNNNGC 1 cut(s) 298
BstNI CCWGG 1 cut(s) 89
BstSCI CCNGG 1 cut(s) 87
BstSFI CTRYAG 1 cut(s) 275
BtgI CCRYGG 1 cut(s) 370
BtsCI GGATG 2 cut(s) 67, 178
Cac8I GCNNGC 1 cut(s) 190
Csp6I GTAC 1 cut(s) 272
CviAII CATG 1 cut(s) 50
CviJI RGCY 6 cut(s) 11, 209, 244, 292, 301, 345
CviKI_1 RGCY 6 cut(s) 11, 209, 244, 292, 301, 345
CviQI GTAC 1 cut(s) 272
DdeI CTNAG 1 cut(s) 120
DpnI GATC 1 cut(s) 225
DpnII GATC 1 cut(s) 223
Eam1104I CTCTTC 1 cut(s) 17
EarI CTCTTC 1 cut(s) 17
Ecl136II GAGCTC 1 cut(s) 11
Eco24I GRGCYC 3 cut(s) 13, 294, 347
Eco32I GATATC 1 cut(s) 377
Eco53kI GAGCTC 1 cut(s) 11
EcoICRI GAGCTC 1 cut(s) 11
EcoRII CCWGG 1 cut(s) 87
EcoRV GATATC 1 cut(s) 377
EcoT38I GRGCYC 3 cut(s) 13, 294, 347
FaeI CATG 1 cut(s) 53
FaiI YATR 3 cut(s) 51, 297, 391
FatI CATG 1 cut(s) 49
FauI CCCGC 1 cut(s) 333
FbaI TGATCA 1 cut(s) 223
FokI GGATG 2 cut(s) 74, 185
FriOI GRGCYC 3 cut(s) 13, 294, 347
FspBI CTAG 3 cut(s) 18, 165, 287
GsuI CTGGAG 1 cut(s) 71
Hin1II CATG 1 cut(s) 53
HinfI GANTC 2 cut(s) 41, 356
Hpy166II GTNNAC 1 cut(s) 154
Hpy188I TCNGA 1 cut(s) 223
Hpy188III TCNNGA 3 cut(s) 95, 179, 239
Hpy8I GTNNAC 1 cut(s) 154
HpyAV CCTTC 2 cut(s) 162, 392
HpyCH4V TGCA 2 cut(s) 277, 314
HpyF10VI GCNNNNNNNGC 1 cut(s) 298
HpyF3I CTNAG 1 cut(s) 120
Hsp92II CATG 1 cut(s) 53
Ksp22I TGATCA 1 cut(s) 223
Kzo9I GATC 1 cut(s) 223
LmnI GCTCC 2 cut(s) 8, 337
LpnPI CCDG 5 cut(s) 74, 101, 174, 234, 306
MaeI CTAG 3 cut(s) 18, 165, 287
MaeIII GTNAC 1 cut(s) 282
MalI GATC 1 cut(s) 225
MbiI CCGCTC 1 cut(s) 340
MboI GATC 1 cut(s) 223
MboII GAAGA 1 cut(s) 34
MhlI GDGCHC 3 cut(s) 13, 294, 347
MluCI AATT 1 cut(s) 231
MroXI GAANNNNTTC 1 cut(s) 45
MseI TTAA 1 cut(s) 137
MspR9I CCNGG 1 cut(s) 89
MvaI CCWGG 1 cut(s) 89
MwoI GCNNNNNNNGC 1 cut(s) 298
NdeII GATC 1 cut(s) 223
NlaIII CATG 1 cut(s) 53
PdmI GAANNNNTTC 1 cut(s) 45
PfeI GAWTC 2 cut(s) 41, 356
PsiI TTATAA 1 cut(s) 391
Psp124BI GAGCTC 1 cut(s) 13
Psp6I CCWGG 1 cut(s) 87
PspGI CCWGG 1 cut(s) 87
PstI CTGCAG 1 cut(s) 279
RsaI GTAC 1 cut(s) 273
RsaNI GTAC 1 cut(s) 272
SacI GAGCTC 1 cut(s) 13
SaqAI TTAA 1 cut(s) 137
Sau3AI GATC 1 cut(s) 223
ScrFI CCNGG 1 cut(s) 89
SduI GDGCHC 3 cut(s) 13, 294, 347
SetI ASST 4 cut(s) 13, 93, 111, 246
SfcI CTRYAG 1 cut(s) 275
SmlI CTYRAG 1 cut(s) 239
SmoI CTYRAG 1 cut(s) 239
Sse9I AATT 1 cut(s) 231
SsiI CCGC 1 cut(s) 340
SspMI CTAG 3 cut(s) 18, 165, 287
SstI GAGCTC 1 cut(s) 13
StyD4I CCNGG 1 cut(s) 87
TasI AATT 1 cut(s) 231
TatI WGTACW 1 cut(s) 271
TfiI GAWTC 2 cut(s) 41, 356
Tru1I TTAA 1 cut(s) 137
Tru9I TTAA 1 cut(s) 137
TspDTI ATGAA 1 cut(s) 38
TspGWI ACGGA 2 cut(s) 188, 359
XmnI GAANNNNTTC 1 cut(s) 45
XspI CTAG 3 cut(s) 18, 165, 287
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.