Rw4G002960

Acetolactate synthase small subunit 1, chloroplastic-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr4
OG0018922
Physical Location & Seq
Reverse (-)
5880432 .. 5881225
794 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw4G002960.1

Sequence Viewer

Length: 261 bp
ATGTTAATAAAGATTGCTGTAAACACTACTGCTAGAAGGGATGTTCTTGAAATTGCTGGCATTTTCCGTGCTAAAGCCGTTGATGTTTCTGATCACACAATTACTCTTGAGCTTACTGGCGATTTGAATAAAATTATTGCACTGCAGAAGTTACTAGAGCCCTATGGGCTTTGCGAGATTGCATGGACTGGGAGAATGGCATTGGAGCTCGGCTCTGGTGTGGATTCCACTTCTCTCCATGGATATCCCCTTCCATTATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00290 ko00650 ko00660 ko00770 ko01100 ko01110 ko01130 ko01210 ko01230 map00290 map00650 map00660 map00770 map01100 map01110 map01130 map01210 map01230
Pfam Domains
Protein Families
ACT ACT_5 ALS_ss_C

Protein Analysis

86

Amino Acids

9.33

Weight (kDa)

5.53

Isoelectric Point (pI)

19.96

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ALS_ss_C PF10369 1 - 69 3.5e-23 Small subunit of acetolactate synthase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0018922)

Species Orthologous Gene IDs
pyrus_communis pycom02g00140
rosa_chinensis RchiOBHm_Chr5g0025461
rosa_multiflora Rmu_co8399419.1_g000001 Rmu_sc0000438.1_g000013 Rmu_sc0016852.1_g000007
rosa_samantha Rh5DG176900
rosa_wichuraiana Rw1G008670 Rw1G025300 Rw4G002960

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 259
AgsI TTSAA 2 cut(s) 50, 127
AluBI AGCT 2 cut(s) 112, 208
AluI AGCT 2 cut(s) 112, 208
Alw21I GWGCWC 1 cut(s) 210
BanII GRGCYC 2 cut(s) 162, 210
Bbv12I GWGCWC 1 cut(s) 210
BceAI ACGGC 1 cut(s) 62
BclI TGATCA 1 cut(s) 91
BfaI CTAG 2 cut(s) 33, 155
BfmI CTRYAG 1 cut(s) 143
BglI GCCNNNNNGGC 1 cut(s) 166
BmrI ACTGGG 1 cut(s) 198
BmuI ACTGGG 1 cut(s) 198
BplI GAGNNNNNCTC 2 cut(s) 197, 229
BpuEI CTTGAG 1 cut(s) 128
BsaJI CCNNGG 1 cut(s) 238
Bse1I ACTGG 2 cut(s) 121, 193
BseDI CCNNGG 1 cut(s) 238
BseGI GGATG 1 cut(s) 46
BseNI ACTGG 2 cut(s) 121, 193
BsiHKAI GWGCWC 1 cut(s) 210
Bsp1286I GDGCHC 2 cut(s) 162, 210
Bsp143I GATC 1 cut(s) 91
Bsp19I CCATGG 1 cut(s) 238
BspMAI CTGCAG 1 cut(s) 147
BsrI ACTGG 2 cut(s) 121, 193
BssECI CCNNGG 1 cut(s) 238
BssMI GATC 1 cut(s) 91
BssT1I CCWWGG 1 cut(s) 238
BstC8I GCNNGC 1 cut(s) 58
BstDSI CCRYGG 1 cut(s) 238
BstF5I GGATG 1 cut(s) 46
BstKTI GATC 1 cut(s) 94
BstMBI GATC 1 cut(s) 91
BstMWI GCNNNNNNNGC 1 cut(s) 166
BstSFI CTRYAG 1 cut(s) 143
BtgI CCRYGG 1 cut(s) 238
BtsCI GGATG 1 cut(s) 46
BtsI GCAGTG 1 cut(s) 140
BtsIMutI CAGTG 1 cut(s) 140
Cac8I GCNNGC 1 cut(s) 58
CviAII CATG 2 cut(s) 183, 239
CviJI RGCY 6 cut(s) 77, 112, 160, 169, 208, 213
CviKI_1 RGCY 6 cut(s) 77, 112, 160, 169, 208, 213
DpnI GATC 1 cut(s) 93
DpnII GATC 1 cut(s) 91
Ecl136II GAGCTC 1 cut(s) 208
Eco130I CCWWGG 1 cut(s) 238
Eco24I GRGCYC 2 cut(s) 162, 210
Eco32I GATATC 1 cut(s) 245
Eco53kI GAGCTC 1 cut(s) 208
EcoICRI GAGCTC 1 cut(s) 208
EcoRV GATATC 1 cut(s) 245
EcoT14I CCWWGG 1 cut(s) 238
EcoT38I GRGCYC 2 cut(s) 162, 210
ErhI CCWWGG 1 cut(s) 238
FaeI CATG 2 cut(s) 186, 242
FaiI YATR 4 cut(s) 165, 184, 240, 259
FatI CATG 2 cut(s) 182, 238
FbaI TGATCA 1 cut(s) 91
FokI GGATG 1 cut(s) 53
FriOI GRGCYC 2 cut(s) 162, 210
FspBI CTAG 2 cut(s) 33, 155
Hin1II CATG 2 cut(s) 186, 242
HinfI GANTC 1 cut(s) 224
Hpy166II GTNNAC 1 cut(s) 22
Hpy188I TCNGA 1 cut(s) 91
Hpy188III TCNNGA 2 cut(s) 47, 107
Hpy8I GTNNAC 1 cut(s) 22
HpyAV CCTTC 2 cut(s) 30, 260
HpyCH4V TGCA 3 cut(s) 140, 145, 182
HpyF10VI GCNNNNNNNGC 1 cut(s) 166
Hsp92II CATG 2 cut(s) 186, 242
Ksp22I TGATCA 1 cut(s) 91
Kzo9I GATC 1 cut(s) 91
LmnI GCTCC 1 cut(s) 205
LpnPI CCDG 4 cut(s) 42, 102, 174, 201
MaeI CTAG 2 cut(s) 33, 155
MaeIII GTNAC 1 cut(s) 150
MalI GATC 1 cut(s) 93
MboI GATC 1 cut(s) 91
MhlI GDGCHC 2 cut(s) 162, 210
MluCI AATT 3 cut(s) 51, 99, 132
MseI TTAA 1 cut(s) 5
MwoI GCNNNNNNNGC 1 cut(s) 166
NcoI CCATGG 1 cut(s) 238
NdeII GATC 1 cut(s) 91
NlaIII CATG 2 cut(s) 186, 242
NmeAIII GCCGAG 1 cut(s) 189
PfeI GAWTC 1 cut(s) 224
PsiI TTATAA 1 cut(s) 259
Psp124BI GAGCTC 1 cut(s) 210
PstI CTGCAG 1 cut(s) 147
SacI GAGCTC 1 cut(s) 210
SaqAI TTAA 1 cut(s) 5
Sau3AI GATC 1 cut(s) 91
SduI GDGCHC 2 cut(s) 162, 210
SetI ASST 2 cut(s) 114, 210
SfcI CTRYAG 1 cut(s) 143
SmlI CTYRAG 1 cut(s) 107
SmoI CTYRAG 1 cut(s) 107
Sse9I AATT 3 cut(s) 51, 99, 132
SspMI CTAG 2 cut(s) 33, 155
SstI GAGCTC 1 cut(s) 210
StyI CCWWGG 1 cut(s) 238
TasI AATT 3 cut(s) 51, 99, 132
TfiI GAWTC 1 cut(s) 224
Tru1I TTAA 1 cut(s) 5
Tru9I TTAA 1 cut(s) 5
TscAI CASTG 1 cut(s) 147
TspGWI ACGGA 1 cut(s) 56
TspRI CASTG 1 cut(s) 147
XspI CTAG 2 cut(s) 33, 155
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.