Rw1G025300

Acetolactate synthase small subunit 1, chloroplastic-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr1
OG0018922
Physical Location & Seq
Reverse (-)
51870028 .. 51870820
793 bp
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UTR
Exon/CDS
Intron
Rw1G025300.1

Sequence Viewer

Length: 294 bp
ATGTTAATAAAGATTGCTGTAAACACTACTACTAGAAGGGATGTTCTTGAAATTGCTGGCATTTTCCGTGCTAAAGCCGTTGATGTTTCTGATCACACAATTACTCTTGAGCTTACTGGCGATTTGAATAAAATTATTGCACTGCAGAAGTTACTAGAGCCCTATGGGCTTTGCGAGTTCCTAGATCTTAACAGTTCTTTACTACTTTCAGATGCATGGACTGGGAGAATGGCATTGGAGCTCGGCTCTGGTGTGGATTCCACTTCTCTCCGTGGATATCCCCTTCCATTATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00290 ko00650 ko00660 ko00770 ko01100 ko01110 ko01130 ko01210 ko01230 map00290 map00650 map00660 map00770 map01100 map01110 map01130 map01210 map01230
Pfam Domains
Protein Families
ACT ACT_5 ALS_ss_C

Protein Analysis

97

Amino Acids

10.59

Weight (kDa)

4.93

Isoelectric Point (pI)

21.01

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ALS_ss_C PF10369 1 - 60 4e-19 Small subunit of acetolactate synthase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0018922)

Species Orthologous Gene IDs
pyrus_communis pycom02g00140
rosa_chinensis RchiOBHm_Chr5g0025461
rosa_multiflora Rmu_co8399419.1_g000001 Rmu_sc0000438.1_g000013 Rmu_sc0016852.1_g000007
rosa_samantha Rh5DG176900
rosa_wichuraiana Rw1G008670 Rw1G025300 Rw4G002960

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 292
AgsI TTSAA 2 cut(s) 50, 127
AluBI AGCT 2 cut(s) 112, 241
AluI AGCT 2 cut(s) 112, 241
Alw21I GWGCWC 1 cut(s) 243
BanII GRGCYC 2 cut(s) 162, 243
Bbv12I GWGCWC 1 cut(s) 243
BceAI ACGGC 1 cut(s) 62
BclI TGATCA 1 cut(s) 91
BfaI CTAG 3 cut(s) 33, 155, 182
BfmI CTRYAG 1 cut(s) 143
BglI GCCNNNNNGGC 1 cut(s) 166
BglII AGATCT 1 cut(s) 184
BmrI ACTGGG 1 cut(s) 231
BmsI GCATC 1 cut(s) 202
BmuI ACTGGG 1 cut(s) 231
BplI GAGNNNNNCTC 2 cut(s) 230, 262
BpuEI CTTGAG 1 cut(s) 128
BsaJI CCNNGG 1 cut(s) 271
Bse1I ACTGG 2 cut(s) 121, 226
BseDI CCNNGG 1 cut(s) 271
BseGI GGATG 1 cut(s) 46
BseNI ACTGG 2 cut(s) 121, 226
BsiHKAI GWGCWC 1 cut(s) 243
Bsp1286I GDGCHC 2 cut(s) 162, 243
Bsp143I GATC 2 cut(s) 91, 184
BspMAI CTGCAG 1 cut(s) 147
BsrI ACTGG 2 cut(s) 121, 226
BssECI CCNNGG 1 cut(s) 271
BssMI GATC 2 cut(s) 91, 184
Bst4CI ACNGT 1 cut(s) 194
BstC8I GCNNGC 1 cut(s) 58
BstDSI CCRYGG 1 cut(s) 271
BstF5I GGATG 1 cut(s) 46
BstKTI GATC 2 cut(s) 94, 187
BstMBI GATC 2 cut(s) 91, 184
BstMWI GCNNNNNNNGC 1 cut(s) 166
BstSFI CTRYAG 1 cut(s) 143
BstX2I RGATCY 1 cut(s) 184
BstYI RGATCY 1 cut(s) 184
BtgI CCRYGG 1 cut(s) 271
BtsCI GGATG 1 cut(s) 46
BtsI GCAGTG 1 cut(s) 140
BtsIMutI CAGTG 1 cut(s) 140
Cac8I GCNNGC 1 cut(s) 58
CviAII CATG 1 cut(s) 216
CviJI RGCY 6 cut(s) 77, 112, 160, 169, 241, 246
CviKI_1 RGCY 6 cut(s) 77, 112, 160, 169, 241, 246
DpnI GATC 2 cut(s) 93, 186
DpnII GATC 2 cut(s) 91, 184
Ecl136II GAGCTC 1 cut(s) 241
Eco24I GRGCYC 2 cut(s) 162, 243
Eco32I GATATC 1 cut(s) 278
Eco53kI GAGCTC 1 cut(s) 241
EcoICRI GAGCTC 1 cut(s) 241
EcoRV GATATC 1 cut(s) 278
EcoT22I ATGCAT 1 cut(s) 217
EcoT38I GRGCYC 2 cut(s) 162, 243
FaeI CATG 1 cut(s) 219
FaiI YATR 3 cut(s) 165, 217, 292
FatI CATG 1 cut(s) 215
FbaI TGATCA 1 cut(s) 91
FokI GGATG 1 cut(s) 53
FriOI GRGCYC 2 cut(s) 162, 243
FspBI CTAG 3 cut(s) 33, 155, 182
Hin1II CATG 1 cut(s) 219
HinfI GANTC 1 cut(s) 257
Hpy166II GTNNAC 1 cut(s) 22
Hpy188I TCNGA 2 cut(s) 91, 211
Hpy188III TCNNGA 2 cut(s) 47, 107
Hpy8I GTNNAC 1 cut(s) 22
HpyAV CCTTC 2 cut(s) 30, 293
HpyCH4III ACNGT 1 cut(s) 194
HpyCH4V TGCA 3 cut(s) 140, 145, 215
HpyF10VI GCNNNNNNNGC 1 cut(s) 166
Hsp92II CATG 1 cut(s) 219
Ksp22I TGATCA 1 cut(s) 91
Kzo9I GATC 2 cut(s) 91, 184
LmnI GCTCC 1 cut(s) 238
LpnPI CCDG 4 cut(s) 42, 102, 207, 234
LweI GCATC 1 cut(s) 202
MaeI CTAG 3 cut(s) 33, 155, 182
MaeIII GTNAC 1 cut(s) 150
MalI GATC 2 cut(s) 93, 186
MboI GATC 2 cut(s) 91, 184
MflI RGATCY 1 cut(s) 184
MhlI GDGCHC 2 cut(s) 162, 243
MluCI AATT 3 cut(s) 51, 99, 132
Mph1103I ATGCAT 1 cut(s) 217
MseI TTAA 2 cut(s) 5, 189
MwoI GCNNNNNNNGC 1 cut(s) 166
NdeII GATC 2 cut(s) 91, 184
NlaIII CATG 1 cut(s) 219
NmeAIII GCCGAG 1 cut(s) 222
NsiI ATGCAT 1 cut(s) 217
PfeI GAWTC 1 cut(s) 257
PsiI TTATAA 1 cut(s) 292
Psp124BI GAGCTC 1 cut(s) 243
PstI CTGCAG 1 cut(s) 147
PsuI RGATCY 1 cut(s) 184
SacI GAGCTC 1 cut(s) 243
SaqAI TTAA 2 cut(s) 5, 189
Sau3AI GATC 2 cut(s) 91, 184
SduI GDGCHC 2 cut(s) 162, 243
SetI ASST 2 cut(s) 114, 243
SfaNI GCATC 1 cut(s) 202
SfcI CTRYAG 1 cut(s) 143
SmlI CTYRAG 1 cut(s) 107
SmoI CTYRAG 1 cut(s) 107
Sse9I AATT 3 cut(s) 51, 99, 132
SspMI CTAG 3 cut(s) 33, 155, 182
SstI GAGCTC 1 cut(s) 243
TaaI ACNGT 1 cut(s) 194
TasI AATT 3 cut(s) 51, 99, 132
TfiI GAWTC 1 cut(s) 257
Tru1I TTAA 2 cut(s) 5, 189
Tru9I TTAA 2 cut(s) 5, 189
TscAI CASTG 1 cut(s) 147
TspGWI ACGGA 2 cut(s) 56, 260
TspRI CASTG 1 cut(s) 147
XspI CTAG 3 cut(s) 33, 155, 182
Zsp2I ATGCAT 1 cut(s) 217
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.