RchiOBHm_Chr5g0047351

MO25-like protein At5g47540

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
5
OG0020559
Physical Location & Seq
Forward (+)
43768655 .. 43771662
3008 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 555 bp
ATGGCTCCTTCGACACCGCCGACGGCGTCTACAACTCGCTTGGTCCGAATCTCACAACTCCGGCCCCCCTCATCGCCTCCGTCAATTTCTTTCGATAATGTTCTTTCCTTCTTCATCTTCATCCTGAACGTAAAGCCACCGAGCACAGACTCAATCAGTTATGGAGACGGTGATATTGCTCTATCTTATGGTGCAATTTTAAGGGAATGCATTCGTCATCAGGTTGTTGCGAGGTACGTCCTGGAATCAGACCACATGAAGAAGTTCTTTGATTATATACAACTTCCAAATTTTGAAATAGCATCGGATGCTGCAGCCACTTTCAAGGAGCTTATGACCAGGCATAAGTCAACAGTTGCACAATTTTTGTCTAATAATTATGACTGGTTTTTCCAAGAATATAATTCGCAGTTGCTGGAGTCTCAGAATTACATCACCAAACGACAGGCTATCAAGATTGGATTGTGGAATTTTTGTGATGTACTACATGAAGAAACCTTCGAAAGAGGAGGGGATTCAGAAGACAATCAGCAAGAAATATATTTTGCAATATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04150 ko04152 map04150 map04152
Pfam Domains
Protein Families
Mo25

Protein Analysis

184

Amino Acids

21.11

Weight (kDa)

5.17

Isoelectric Point (pI)

54.83

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Mo25 PF08569 54 - 153 2e-42 Mo25-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0020559)

Species Orthologous Gene IDs
rosa_chinensis RchiOBHm_Chr5g0047351
rosa_roxburghii Rroxscaffold_3G00262450
rosa_samantha Rh5BG323600 Rh5DG334600
rosa_wichuraiana Rw0G014620 Rw2G019020

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 29
AciI CCGC 1 cut(s) 17
AcsI RAATTY 2 cut(s) 289, 469
AcyI GRCGYC 1 cut(s) 26
AfaI GTAC 2 cut(s) 236, 483
AgsI TTSAA 2 cut(s) 296, 325
AjnI CCWGG 2 cut(s) 240, 338
AluBI AGCT 1 cut(s) 331
AluI AGCT 1 cut(s) 331
Alw21I GWGCWC 1 cut(s) 146
Alw26I GTCTC 2 cut(s) 159, 426
AlwNI CAGNNNCTG 1 cut(s) 415
AoxI GGCC 1 cut(s) 62
ApeKI GCWGC 2 cut(s) 311, 314
ApoI RAATTY 2 cut(s) 289, 469
Asp700I GAANNNNTTC 2 cut(s) 210, 263
AspS9I GGNCC 2 cut(s) 43, 63
AsuHPI GGTGA 2 cut(s) 182, 427
AsuII TTCGAA 1 cut(s) 501
AvaII GGWCC 1 cut(s) 43
BbsI GAAGAC 1 cut(s) 528
Bbv12I GWGCWC 1 cut(s) 146
BbvI GCAGC 2 cut(s) 298, 326
BceAI ACGGC 1 cut(s) 39
BciT130I CCWGG 2 cut(s) 242, 340
BcoDI GTCTC 2 cut(s) 159, 426
BfmI CTRYAG 1 cut(s) 312
BisI GCNGC 2 cut(s) 312, 315
BlsI GCNGC 2 cut(s) 313, 316
Bme1390I CCNGG 2 cut(s) 242, 340
Bme18I GGWCC 1 cut(s) 43
BmgT120I GGNCC 2 cut(s) 43, 63
BmiI GGNNCC 2 cut(s) 6, 65
BmrFI CCNGG 2 cut(s) 242, 340
BmsI GCATC 2 cut(s) 298, 311
BpiI GAAGAC 1 cut(s) 528
BpmI CTGGAG 1 cut(s) 437
Bpu14I TTCGAA 1 cut(s) 501
BsaHI GRCGYC 1 cut(s) 26
Bse1I ACTGG 1 cut(s) 389
BseBI CCWGG 2 cut(s) 242, 340
BseGI GGATG 2 cut(s) 120, 313
BseMII CTCAG 1 cut(s) 437
BseNI ACTGG 1 cut(s) 389
BseRI GAGGAG 1 cut(s) 522
BseXI GCAGC 2 cut(s) 298, 326
BshFI GGCC 1 cut(s) 64
BsiHKAI GWGCWC 1 cut(s) 146
BsiSI CCGG 1 cut(s) 61
BsmAI GTCTC 2 cut(s) 159, 426
BsmBI CGTCTC 1 cut(s) 159
BsmI GAATGC 2 cut(s) 210, 212
BsnI GGCC 1 cut(s) 64
Bsp119I TTCGAA 1 cut(s) 501
Bsp1286I GDGCHC 1 cut(s) 146
BspACI CCGC 1 cut(s) 17
BspANI GGCC 1 cut(s) 64
BspCNI CTCAG 1 cut(s) 436
BspLI GGNNCC 2 cut(s) 6, 65
BspMAI CTGCAG 1 cut(s) 316
BspT104I TTCGAA 1 cut(s) 501
BsrI ACTGG 1 cut(s) 389
BssNI GRCGYC 1 cut(s) 26
Bst2UI CCWGG 2 cut(s) 242, 340
Bst4CI ACNGT 2 cut(s) 170, 355
BstACI GRCGYC 1 cut(s) 26
BstAPI GCANNNNNTGC 1 cut(s) 308
BstBI TTCGAA 1 cut(s) 501
BstDEI CTNAG 1 cut(s) 423
BstF5I GGATG 2 cut(s) 120, 313
BstMAI GTCTC 2 cut(s) 159, 426
BstMWI GCNNNNNNNGC 1 cut(s) 308
BstNI CCWGG 2 cut(s) 242, 340
BstSCI CCNGG 2 cut(s) 240, 338
BstSFI CTRYAG 1 cut(s) 312
BstV1I GCAGC 2 cut(s) 298, 326
BstV2I GAAGAC 1 cut(s) 528
BsuRI GGCC 1 cut(s) 64
BtgZI GCGATG 1 cut(s) 57
BtsCI GGATG 2 cut(s) 120, 313
CaiI CAGNNNCTG 1 cut(s) 415
Cfr13I GGNCC 2 cut(s) 43, 63
CseI GACGC 1 cut(s) 15
Csp6I GTAC 2 cut(s) 235, 482
CviAII CATG 2 cut(s) 256, 488
CviJI RGCY 6 cut(s) 5, 64, 136, 317, 331, 449
CviKI_1 RGCY 6 cut(s) 5, 64, 136, 317, 331, 449
CviQI GTAC 2 cut(s) 235, 482
DdeI CTNAG 1 cut(s) 423
Eco47I GGWCC 1 cut(s) 43
EcoRII CCWGG 2 cut(s) 240, 338
EcoT22I ATGCAT 1 cut(s) 212
Esp3I CGTCTC 1 cut(s) 159
FaeI CATG 2 cut(s) 259, 491
FalI AAGNNNNNCTT 2 cut(s) 251, 283
FatI CATG 2 cut(s) 255, 487
FblI GTMKAC 1 cut(s) 29
Fnu4HI GCNGC 2 cut(s) 312, 315
FokI GGATG 2 cut(s) 107, 320
Fsp4HI GCNGC 2 cut(s) 312, 315
GluI GCNGC 2 cut(s) 312, 315
GsuI CTGGAG 1 cut(s) 437
HaeIII GGCC 1 cut(s) 64
HapII CCGG 1 cut(s) 61
HgaI GACGC 1 cut(s) 15
Hin1I GRCGYC 1 cut(s) 26
Hin1II CATG 2 cut(s) 259, 491
HincII GTYRAC 1 cut(s) 351
HindII GTYRAC 1 cut(s) 351
HinfI GANTC 5 cut(s) 48, 149, 245, 419, 515
HpaII CCGG 1 cut(s) 61
HphI GGTGA 2 cut(s) 182, 427
Hpy166II GTNNAC 2 cut(s) 30, 351
Hpy188I TCNGA 5 cut(s) 47, 250, 307, 426, 520
Hpy188III TCNNGA 2 cut(s) 124, 454
Hpy8I GTNNAC 2 cut(s) 30, 351
Hpy99I CGWCG 1 cut(s) 25
HpyAV CCTTC 3 cut(s) 18, 118, 508
HpyCH4III ACNGT 2 cut(s) 170, 355
HpyCH4IV ACGT 2 cut(s) 129, 237
HpyCH4V TGCA 5 cut(s) 194, 210, 314, 359, 548
HpyF10VI GCNNNNNNNGC 1 cut(s) 308
HpyF3I CTNAG 1 cut(s) 423
HpySE526I ACGT 2 cut(s) 129, 237
Hsp92I GRCGYC 1 cut(s) 26
Hsp92II CATG 2 cut(s) 259, 491
LmnI GCTCC 2 cut(s) 10, 328
Lsp1109I GCAGC 2 cut(s) 298, 326
LweI GCATC 2 cut(s) 298, 311
MaeII ACGT 2 cut(s) 129, 237
MboII GAAGA 5 cut(s) 103, 109, 271, 503, 533
MhlI GDGCHC 1 cut(s) 146
MluCI AATT 8 cut(s) 84, 195, 289, 362, 376, 403, 427, 469
MlyI GAGTC 2 cut(s) 143, 428
MnlI CCTC 5 cut(s) 79, 87, 225, 500, 503
Mph1103I ATGCAT 1 cut(s) 212
MroXI GAANNNNTTC 2 cut(s) 210, 263
MseI TTAA 1 cut(s) 200
MspI CCGG 1 cut(s) 61
MspR9I CCNGG 2 cut(s) 242, 340
Mva1269I GAATGC 2 cut(s) 210, 212
MvaI CCWGG 2 cut(s) 242, 340
MwoI GCNNNNNNNGC 1 cut(s) 308
NlaIII CATG 2 cut(s) 259, 491
NlaIV GGNNCC 2 cut(s) 6, 65
NsiI ATGCAT 1 cut(s) 212
NspV TTCGAA 1 cut(s) 501
PcsI WCGNNNNNNNCGW 2 cut(s) 17, 43
PctI GAATGC 2 cut(s) 210, 212
PdmI GAANNNNTTC 2 cut(s) 210, 263
PfeI GAWTC 3 cut(s) 48, 245, 515
PflFI GACNNNGTC 1 cut(s) 25
PfoI TCCNGGA 1 cut(s) 240
PkrI GCNGC 2 cut(s) 313, 316
PleI GAGTC 2 cut(s) 143, 427
PpsI GAGTC 2 cut(s) 143, 427
Psp6I CCWGG 2 cut(s) 240, 338
PspGI CCWGG 2 cut(s) 240, 338
PspN4I GGNNCC 2 cut(s) 6, 65
PspPI GGNCC 2 cut(s) 43, 63
PstI CTGCAG 1 cut(s) 316
PstNI CAGNNNCTG 1 cut(s) 415
PsyI GACNNNGTC 1 cut(s) 25
RsaI GTAC 2 cut(s) 236, 483
RsaNI GTAC 2 cut(s) 235, 482
SaqAI TTAA 1 cut(s) 200
SatI GCNGC 2 cut(s) 312, 315
Sau96I GGNCC 2 cut(s) 43, 63
SchI GAGTC 2 cut(s) 143, 428
ScrFI CCNGG 2 cut(s) 242, 340
SduI GDGCHC 1 cut(s) 146
SetI ASST 6 cut(s) 132, 225, 236, 240, 333, 500
SfaNI GCATC 2 cut(s) 298, 311
SfcI CTRYAG 1 cut(s) 312
SfuI TTCGAA 1 cut(s) 501
SinI GGWCC 1 cut(s) 43
Sse9I AATT 8 cut(s) 84, 195, 289, 362, 376, 403, 427, 469
SsiI CCGC 1 cut(s) 17
StyD4I CCNGG 2 cut(s) 240, 338
TaaI ACNGT 2 cut(s) 170, 355
TaiI ACGT 2 cut(s) 132, 240
TaqI TCGA 3 cut(s) 11, 93, 501
TasI AATT 8 cut(s) 84, 195, 289, 362, 376, 403, 427, 469
TatI WGTACW 1 cut(s) 481
TfiI GAWTC 3 cut(s) 48, 245, 515
Tru1I TTAA 1 cut(s) 200
Tru9I TTAA 1 cut(s) 200
TseI GCWGC 2 cut(s) 311, 314
TspDTI ATGAA 4 cut(s) 103, 109, 272, 504
TspGWI ACGGA 1 cut(s) 69
Tth111I GACNNNGTC 1 cut(s) 25
VpaK11BI GGWCC 1 cut(s) 43
XapI RAATTY 2 cut(s) 289, 469
XmiI GTMKAC 1 cut(s) 29
XmnI GAANNNNTTC 2 cut(s) 210, 263
Zsp2I ATGCAT 1 cut(s) 212
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.