Rh5BG323600

MO25-like protein At5g47540

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5B
OG0020559
Physical Location & Seq
Forward (+)
43595232 .. 43598292
3061 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5BG323600.1

Sequence Viewer

Length: 561 bp
ATGGCTCCTTCGACACCGCCGACGGCGTCTACAACTCGCTTGGTCCGAATCTCACAACTCCGGCCCCCCTCATCGCCTCCGTCAATTTCTTTCGATAATGTTCTTTCCTTCTTCATCTTCATCCTGAACGTAAAGCCACCGAGCACAGACTCAATCAGTTATGGAGACGGTGATATTGCTCTATCTTATGGTGCAATTTTAAGGGAATGCATTCGTCATCAGGTTGTTGCGAGGTACGTCCTGGAATCAGACCACATGAAGAAGTTCTTTGATTATATACAACTTCCAAATTTTGAAATAGCATCGGATGCTGCAGCCACTTTCAAGGAGCTTATGACCAGGCATAAGTCAACAGTTGCACAATTTTTGTCTAATAATTATGACTGGTTTTTCCAAGAATATAATTCGCAGTTGCTGGAGTCTCAGAATTACATCACCAAACGACAGGCTATCAAGGTAAGTATACATAGTTTTTGTGCCCCCGAGAGTCTTGTTTTTGTCATCCAATTTTCTGTAATATATTCATTGCTGAGATTTATTTTGCTAAAAGAACAGGAATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04150 ko04152 map04150 map04152
Pfam Domains
Protein Families
Mo25

Protein Analysis

186

Amino Acids

21.29

Weight (kDa)

6.91

Isoelectric Point (pI)

50.7

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Mo25 PF08569 54 - 153 4.4e-42 Mo25-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0020559)

Species Orthologous Gene IDs
rosa_chinensis RchiOBHm_Chr5g0047351
rosa_roxburghii Rroxscaffold_3G00262450
rosa_samantha Rh5BG323600 Rh5DG334600
rosa_wichuraiana Rw0G014620 Rw2G019020

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 2 cut(s) 29, 463
AciI CCGC 1 cut(s) 17
AcsI RAATTY 1 cut(s) 289
AcyI GRCGYC 1 cut(s) 26
AfaI GTAC 1 cut(s) 236
AgsI TTSAA 2 cut(s) 296, 325
AjnI CCWGG 2 cut(s) 240, 338
AluBI AGCT 1 cut(s) 331
AluI AGCT 1 cut(s) 331
Alw21I GWGCWC 1 cut(s) 146
Alw26I GTCTC 2 cut(s) 159, 426
AlwNI CAGNNNCTG 1 cut(s) 415
Ama87I CYCGRG 1 cut(s) 482
AoxI GGCC 1 cut(s) 62
ApeKI GCWGC 2 cut(s) 311, 314
ApoI RAATTY 1 cut(s) 289
Asp700I GAANNNNTTC 2 cut(s) 210, 263
AspS9I GGNCC 2 cut(s) 43, 63
AsuHPI GGTGA 2 cut(s) 182, 427
AvaI CYCGRG 1 cut(s) 482
AvaII GGWCC 1 cut(s) 43
BaeGI GKGCMC 1 cut(s) 481
Bbv12I GWGCWC 1 cut(s) 146
BbvI GCAGC 2 cut(s) 298, 326
BceAI ACGGC 1 cut(s) 39
BciT130I CCWGG 2 cut(s) 242, 340
BcoDI GTCTC 2 cut(s) 159, 426
BfmI CTRYAG 1 cut(s) 312
BisI GCNGC 2 cut(s) 312, 315
BlsI GCNGC 2 cut(s) 313, 316
Bme1390I CCNGG 2 cut(s) 242, 340
Bme18I GGWCC 1 cut(s) 43
BmeT110I CYCGRG 1 cut(s) 482
BmgT120I GGNCC 2 cut(s) 43, 63
BmiI GGNNCC 2 cut(s) 6, 65
BmrFI CCNGG 2 cut(s) 242, 340
BmsI GCATC 2 cut(s) 298, 311
BpmI CTGGAG 1 cut(s) 437
BsaHI GRCGYC 1 cut(s) 26
Bse1I ACTGG 1 cut(s) 389
Bse3DI GCAATG 1 cut(s) 524
BseBI CCWGG 2 cut(s) 242, 340
BseGI GGATG 3 cut(s) 120, 313, 501
BseMI GCAATG 1 cut(s) 524
BseMII CTCAG 2 cut(s) 437, 521
BseNI ACTGG 1 cut(s) 389
BseSI GKGCMC 1 cut(s) 481
BseXI GCAGC 2 cut(s) 298, 326
BshFI GGCC 1 cut(s) 64
BsiHKAI GWGCWC 1 cut(s) 146
BsiHKCI CYCGRG 1 cut(s) 482
BsiSI CCGG 1 cut(s) 61
BsmAI GTCTC 2 cut(s) 159, 426
BsmBI CGTCTC 1 cut(s) 159
BsmI GAATGC 2 cut(s) 210, 212
BsnI GGCC 1 cut(s) 64
BsoBI CYCGRG 1 cut(s) 482
Bsp1286I GDGCHC 2 cut(s) 146, 481
BspACI CCGC 1 cut(s) 17
BspANI GGCC 1 cut(s) 64
BspCNI CTCAG 2 cut(s) 436, 522
BspLI GGNNCC 2 cut(s) 6, 65
BspMAI CTGCAG 1 cut(s) 316
BsrDI GCAATG 1 cut(s) 524
BsrI ACTGG 1 cut(s) 389
BssNAI GTATAC 1 cut(s) 464
BssNI GRCGYC 1 cut(s) 26
Bst1107I GTATAC 1 cut(s) 464
Bst2UI CCWGG 2 cut(s) 242, 340
Bst4CI ACNGT 2 cut(s) 170, 355
BstACI GRCGYC 1 cut(s) 26
BstAPI GCANNNNNTGC 1 cut(s) 308
BstDEI CTNAG 2 cut(s) 423, 530
BstF5I GGATG 3 cut(s) 120, 313, 501
BstMAI GTCTC 2 cut(s) 159, 426
BstMWI GCNNNNNNNGC 1 cut(s) 308
BstNI CCWGG 2 cut(s) 242, 340
BstSCI CCNGG 2 cut(s) 240, 338
BstSFI CTRYAG 1 cut(s) 312
BstSLI GKGCMC 1 cut(s) 481
BstV1I GCAGC 2 cut(s) 298, 326
BstZ17I GTATAC 1 cut(s) 464
BsuRI GGCC 1 cut(s) 64
BtgZI GCGATG 1 cut(s) 57
BtsCI GGATG 3 cut(s) 120, 313, 501
CaiI CAGNNNCTG 1 cut(s) 415
Cfr13I GGNCC 2 cut(s) 43, 63
CseI GACGC 1 cut(s) 15
Csp6I GTAC 1 cut(s) 235
CviAII CATG 1 cut(s) 256
CviJI RGCY 6 cut(s) 5, 64, 136, 317, 331, 449
CviKI_1 RGCY 6 cut(s) 5, 64, 136, 317, 331, 449
CviQI GTAC 1 cut(s) 235
DdeI CTNAG 2 cut(s) 423, 530
Eco47I GGWCC 1 cut(s) 43
Eco88I CYCGRG 1 cut(s) 482
EcoRII CCWGG 2 cut(s) 240, 338
EcoT22I ATGCAT 1 cut(s) 212
Esp3I CGTCTC 1 cut(s) 159
FaeI CATG 1 cut(s) 259
FalI AAGNNNNNCTT 2 cut(s) 251, 283
FatI CATG 1 cut(s) 255
FblI GTMKAC 2 cut(s) 29, 463
Fnu4HI GCNGC 2 cut(s) 312, 315
FokI GGATG 3 cut(s) 107, 320, 488
Fsp4HI GCNGC 2 cut(s) 312, 315
GluI GCNGC 2 cut(s) 312, 315
GsuI CTGGAG 1 cut(s) 437
HaeIII GGCC 1 cut(s) 64
HapII CCGG 1 cut(s) 61
HgaI GACGC 1 cut(s) 15
Hin1I GRCGYC 1 cut(s) 26
Hin1II CATG 1 cut(s) 259
HincII GTYRAC 1 cut(s) 351
HindII GTYRAC 1 cut(s) 351
HinfI GANTC 5 cut(s) 48, 149, 245, 419, 487
HpaII CCGG 1 cut(s) 61
HphI GGTGA 2 cut(s) 182, 427
Hpy166II GTNNAC 3 cut(s) 30, 351, 464
Hpy188I TCNGA 4 cut(s) 47, 250, 307, 426
Hpy188III TCNNGA 1 cut(s) 124
Hpy8I GTNNAC 3 cut(s) 30, 351, 464
Hpy99I CGWCG 1 cut(s) 25
HpyAV CCTTC 2 cut(s) 18, 118
HpyCH4III ACNGT 2 cut(s) 170, 355
HpyCH4IV ACGT 2 cut(s) 129, 237
HpyCH4V TGCA 4 cut(s) 194, 210, 314, 359
HpyF10VI GCNNNNNNNGC 1 cut(s) 308
HpyF3I CTNAG 2 cut(s) 423, 530
HpySE526I ACGT 2 cut(s) 129, 237
Hsp92I GRCGYC 1 cut(s) 26
Hsp92II CATG 1 cut(s) 259
LmnI GCTCC 2 cut(s) 10, 328
Lsp1109I GCAGC 2 cut(s) 298, 326
LweI GCATC 2 cut(s) 298, 311
MaeII ACGT 2 cut(s) 129, 237
MboII GAAGA 3 cut(s) 103, 109, 271
MhlI GDGCHC 2 cut(s) 146, 481
MluCI AATT 8 cut(s) 84, 195, 289, 362, 376, 403, 427, 506
MlyI GAGTC 3 cut(s) 143, 428, 496
MnlI CCTC 3 cut(s) 79, 87, 225
Mph1103I ATGCAT 1 cut(s) 212
MroXI GAANNNNTTC 2 cut(s) 210, 263
MseI TTAA 1 cut(s) 200
MspI CCGG 1 cut(s) 61
MspR9I CCNGG 2 cut(s) 242, 340
Mva1269I GAATGC 2 cut(s) 210, 212
MvaI CCWGG 2 cut(s) 242, 340
MwoI GCNNNNNNNGC 1 cut(s) 308
NlaIII CATG 1 cut(s) 259
NlaIV GGNNCC 2 cut(s) 6, 65
NsiI ATGCAT 1 cut(s) 212
PcsI WCGNNNNNNNCGW 2 cut(s) 17, 43
PctI GAATGC 2 cut(s) 210, 212
PdmI GAANNNNTTC 2 cut(s) 210, 263
PfeI GAWTC 2 cut(s) 48, 245
PflFI GACNNNGTC 1 cut(s) 25
PfoI TCCNGGA 1 cut(s) 240
PkrI GCNGC 2 cut(s) 313, 316
PleI GAGTC 3 cut(s) 143, 427, 495
PpsI GAGTC 3 cut(s) 143, 427, 495
Psp6I CCWGG 2 cut(s) 240, 338
PspGI CCWGG 2 cut(s) 240, 338
PspN4I GGNNCC 2 cut(s) 6, 65
PspPI GGNCC 2 cut(s) 43, 63
PstI CTGCAG 1 cut(s) 316
PstNI CAGNNNCTG 1 cut(s) 415
PsyI GACNNNGTC 1 cut(s) 25
RsaI GTAC 1 cut(s) 236
RsaNI GTAC 1 cut(s) 235
SaqAI TTAA 1 cut(s) 200
SatI GCNGC 2 cut(s) 312, 315
Sau96I GGNCC 2 cut(s) 43, 63
SchI GAGTC 3 cut(s) 143, 428, 496
ScrFI CCNGG 2 cut(s) 242, 340
SduI GDGCHC 2 cut(s) 146, 481
SetI ASST 6 cut(s) 132, 225, 236, 240, 333, 459
SfaNI GCATC 2 cut(s) 298, 311
SfcI CTRYAG 1 cut(s) 312
SinI GGWCC 1 cut(s) 43
Sse9I AATT 8 cut(s) 84, 195, 289, 362, 376, 403, 427, 506
SsiI CCGC 1 cut(s) 17
StyD4I CCNGG 2 cut(s) 240, 338
TaaI ACNGT 2 cut(s) 170, 355
TaiI ACGT 2 cut(s) 132, 240
TaqI TCGA 2 cut(s) 11, 93
TasI AATT 8 cut(s) 84, 195, 289, 362, 376, 403, 427, 506
TfiI GAWTC 2 cut(s) 48, 245
Tru1I TTAA 1 cut(s) 200
Tru9I TTAA 1 cut(s) 200
TseI GCWGC 2 cut(s) 311, 314
TspDTI ATGAA 4 cut(s) 103, 109, 272, 513
TspGWI ACGGA 1 cut(s) 69
Tth111I GACNNNGTC 1 cut(s) 25
VpaK11BI GGWCC 1 cut(s) 43
XapI RAATTY 1 cut(s) 289
XmiI GTMKAC 2 cut(s) 29, 463
XmnI GAANNNNTTC 2 cut(s) 210, 263
Zsp2I ATGCAT 1 cut(s) 212
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.