Rroxscaffold_3G00262450

MO25-like protein At5g47540

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000003
OG0020559
Physical Location & Seq
Reverse (-)
56451337 .. 56453483
2147 bp
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UTR
Exon/CDS
Intron
Rroxscaffold_3G00262450.1

Sequence Viewer

Length: 297 bp
ATGAAGTTCTTTGATTATATACAACTTCCAAATTTTGAAATAGCATTGGATGCTGCAGCCACTTTCAAGGAGCTTATGACCAGGCATAAGTCAACAGTTGCACAATTTTTGTCTAATAATTATGACTGGTTTTTCCAAGAATATAATTCGCAGTTGCTGGAGTCTCGTAATTACATCACCAAACAACAGGCTATCAAGATTGGATTGTGGAATTTTTGTGATGTACTACATGAAGAAACCTTCGAAAGAGGAGGGGATTCAGAAGACAATCAGCAAGAAATATATTTTGCAATATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04150 ko04152 map04150 map04152
Pfam Domains
Protein Families
Mo25

Protein Analysis

98

Amino Acids

11.71

Weight (kDa)

4.61

Isoelectric Point (pI)

50.89

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Mo25 PF08569 2 - 67 4e-25 Mo25-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0020559)

Species Orthologous Gene IDs
rosa_chinensis RchiOBHm_Chr5g0047351
rosa_roxburghii Rroxscaffold_3G00262450
rosa_samantha Rh5BG323600 Rh5DG334600
rosa_wichuraiana Rw0G014620 Rw2G019020

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AcsI RAATTY 2 cut(s) 31, 211
AfaI GTAC 1 cut(s) 225
AgsI TTSAA 2 cut(s) 38, 67
AjnI CCWGG 1 cut(s) 80
AluBI AGCT 1 cut(s) 73
AluI AGCT 1 cut(s) 73
Alw26I GTCTC 1 cut(s) 168
AlwNI CAGNNNCTG 1 cut(s) 157
ApeKI GCWGC 2 cut(s) 53, 56
ApoI RAATTY 2 cut(s) 31, 211
AsuHPI GGTGA 1 cut(s) 169
AsuII TTCGAA 1 cut(s) 243
BbsI GAAGAC 1 cut(s) 270
BbvI GCAGC 2 cut(s) 40, 68
BciT130I CCWGG 1 cut(s) 82
BcoDI GTCTC 1 cut(s) 168
BfmI CTRYAG 1 cut(s) 54
BisI GCNGC 2 cut(s) 54, 57
BlsI GCNGC 2 cut(s) 55, 58
Bme1390I CCNGG 1 cut(s) 82
BmrFI CCNGG 1 cut(s) 82
BmsI GCATC 1 cut(s) 40
BpiI GAAGAC 1 cut(s) 270
BpmI CTGGAG 1 cut(s) 179
Bpu14I TTCGAA 1 cut(s) 243
Bse1I ACTGG 1 cut(s) 131
BseBI CCWGG 1 cut(s) 82
BseGI GGATG 1 cut(s) 55
BseNI ACTGG 1 cut(s) 131
BseRI GAGGAG 1 cut(s) 264
BseXI GCAGC 2 cut(s) 40, 68
BsmAI GTCTC 1 cut(s) 168
Bsp119I TTCGAA 1 cut(s) 243
BspMAI CTGCAG 1 cut(s) 58
BspT104I TTCGAA 1 cut(s) 243
BsrI ACTGG 1 cut(s) 131
Bst2UI CCWGG 1 cut(s) 82
Bst4CI ACNGT 1 cut(s) 97
BstAPI GCANNNNNTGC 1 cut(s) 50
BstBI TTCGAA 1 cut(s) 243
BstF5I GGATG 1 cut(s) 55
BstMAI GTCTC 1 cut(s) 168
BstMWI GCNNNNNNNGC 1 cut(s) 50
BstNI CCWGG 1 cut(s) 82
BstSCI CCNGG 1 cut(s) 80
BstSFI CTRYAG 1 cut(s) 54
BstV1I GCAGC 2 cut(s) 40, 68
BstV2I GAAGAC 1 cut(s) 270
BtsCI GGATG 1 cut(s) 55
CaiI CAGNNNCTG 1 cut(s) 157
Csp6I GTAC 1 cut(s) 224
CviAII CATG 1 cut(s) 230
CviJI RGCY 3 cut(s) 59, 73, 191
CviKI_1 RGCY 3 cut(s) 59, 73, 191
CviQI GTAC 1 cut(s) 224
EcoRII CCWGG 1 cut(s) 80
FaeI CATG 1 cut(s) 233
FaiI YATR 9 cut(s) 18, 20, 77, 87, 123, 144, 231, 283, 295
FatI CATG 1 cut(s) 229
Fnu4HI GCNGC 2 cut(s) 54, 57
FokI GGATG 1 cut(s) 62
Fsp4HI GCNGC 2 cut(s) 54, 57
GluI GCNGC 2 cut(s) 54, 57
GsuI CTGGAG 1 cut(s) 179
Hin1II CATG 1 cut(s) 233
HincII GTYRAC 1 cut(s) 93
HindII GTYRAC 1 cut(s) 93
HinfI GANTC 2 cut(s) 161, 257
HphI GGTGA 1 cut(s) 169
Hpy166II GTNNAC 1 cut(s) 93
Hpy188I TCNGA 1 cut(s) 262
Hpy188III TCNNGA 1 cut(s) 196
Hpy8I GTNNAC 1 cut(s) 93
HpyAV CCTTC 1 cut(s) 250
HpyCH4III ACNGT 1 cut(s) 97
HpyCH4V TGCA 3 cut(s) 56, 101, 290
HpyF10VI GCNNNNNNNGC 1 cut(s) 50
Hsp92II CATG 1 cut(s) 233
LmnI GCTCC 1 cut(s) 70
LpnPI CCDG 5 cut(s) 67, 94, 112, 143, 173
Lsp1109I GCAGC 2 cut(s) 40, 68
LweI GCATC 1 cut(s) 40
MboII GAAGA 2 cut(s) 245, 275
MluCI AATT 6 cut(s) 31, 104, 118, 145, 169, 211
MlyI GAGTC 1 cut(s) 170
MnlI CCTC 2 cut(s) 242, 245
MspR9I CCNGG 1 cut(s) 82
MvaI CCWGG 1 cut(s) 82
MwoI GCNNNNNNNGC 1 cut(s) 50
NlaIII CATG 1 cut(s) 233
NspV TTCGAA 1 cut(s) 243
PfeI GAWTC 1 cut(s) 257
PkrI GCNGC 2 cut(s) 55, 58
PleI GAGTC 1 cut(s) 169
PpsI GAGTC 1 cut(s) 169
Psp6I CCWGG 1 cut(s) 80
PspGI CCWGG 1 cut(s) 80
PstI CTGCAG 1 cut(s) 58
PstNI CAGNNNCTG 1 cut(s) 157
RsaI GTAC 1 cut(s) 225
RsaNI GTAC 1 cut(s) 224
SatI GCNGC 2 cut(s) 54, 57
SchI GAGTC 1 cut(s) 170
ScrFI CCNGG 1 cut(s) 82
SetI ASST 2 cut(s) 75, 242
SfaNI GCATC 1 cut(s) 40
SfcI CTRYAG 1 cut(s) 54
SfuI TTCGAA 1 cut(s) 243
Sse9I AATT 6 cut(s) 31, 104, 118, 145, 169, 211
StyD4I CCNGG 1 cut(s) 80
TaaI ACNGT 1 cut(s) 97
TaqI TCGA 1 cut(s) 243
TasI AATT 6 cut(s) 31, 104, 118, 145, 169, 211
TatI WGTACW 1 cut(s) 223
TfiI GAWTC 1 cut(s) 257
TseI GCWGC 2 cut(s) 53, 56
TspDTI ATGAA 2 cut(s) 17, 246
XapI RAATTY 2 cut(s) 31, 211
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.