Rh5DG334600

MO25-like protein At5g47540

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5D
OG0020559
Physical Location & Seq
Forward (+)
44360162 .. 44365003
4842 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5DG334600.1

Sequence Viewer

Length: 537 bp
ATGGCTCCTTCGACACCGCCGACGGCGTCTACAACTCGCTTGGTCCGAATCTCACAACTCCGGCCCCCCTCATCGCCTCCGTCAATTTCTTTCGATAATGTTCTTTCCTTCTTCATCTTCATCCTGAACGTAAAGCCACCGAGCACAGACTCAATCAGTTATGGAGACGGTGATATTGCTCTATCTTATGGTGCAATTTTAAGGGAATGCATTCGTCATCAGGTTGTTGCGAGGTACGTCCTGGAATCAGACCACATGAAGAAGTTCTTTGATTATATACAACTTCCAAATTTTGAAATAGCATCGGATGCTGCAGCCACTTTCAAGGAGCTTATGACCAGGCATAAGTCAACAGTTGCACAATTTTTGTCTAATAATTATGACTGGTTTTTCCAAGAATATAATTCGCAGTTGCTGGAGTCTCAGAATTACATCACCAAACGACAGGCTATCAAGGCTTTCAAGCAAGAGATGAAGCAGGTCCAAGCACCAAAGAAGTTTCAAAAGGTCAAAGATTGTTTACTTATATCAATGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04150 ko04152 map04150 map04152
Pfam Domains
Protein Families
Mo25

Protein Analysis

178

Amino Acids

20.4

Weight (kDa)

9.21

Isoelectric Point (pI)

49.74

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Mo25 PF08569 54 - 153 3.3e-42 Mo25-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0020559)

Species Orthologous Gene IDs
rosa_chinensis RchiOBHm_Chr5g0047351
rosa_roxburghii Rroxscaffold_3G00262450
rosa_samantha Rh5BG323600 Rh5DG334600
rosa_wichuraiana Rw0G014620 Rw2G019020

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 469
AccI GTMKAC 1 cut(s) 29
AciI CCGC 1 cut(s) 17
AcsI RAATTY 1 cut(s) 289
AcyI GRCGYC 1 cut(s) 26
AfaI GTAC 1 cut(s) 236
AgsI TTSAA 4 cut(s) 296, 325, 463, 503
AjnI CCWGG 2 cut(s) 240, 338
AluBI AGCT 1 cut(s) 331
AluI AGCT 1 cut(s) 331
Alw21I GWGCWC 1 cut(s) 146
Alw26I GTCTC 2 cut(s) 159, 426
AlwNI CAGNNNCTG 1 cut(s) 415
AoxI GGCC 1 cut(s) 62
ApeKI GCWGC 2 cut(s) 311, 314
ApoI RAATTY 1 cut(s) 289
Asp700I GAANNNNTTC 2 cut(s) 210, 263
AspS9I GGNCC 3 cut(s) 43, 63, 481
AsuHPI GGTGA 2 cut(s) 182, 427
AvaII GGWCC 2 cut(s) 43, 481
Bbv12I GWGCWC 1 cut(s) 146
BbvI GCAGC 2 cut(s) 298, 326
BceAI ACGGC 1 cut(s) 39
BciT130I CCWGG 2 cut(s) 242, 340
BcoDI GTCTC 2 cut(s) 159, 426
BfmI CTRYAG 1 cut(s) 312
BfuAI ACCTGC 1 cut(s) 469
BisI GCNGC 2 cut(s) 312, 315
BlsI GCNGC 2 cut(s) 313, 316
Bme1390I CCNGG 2 cut(s) 242, 340
Bme18I GGWCC 2 cut(s) 43, 481
BmgT120I GGNCC 3 cut(s) 43, 63, 481
BmiI GGNNCC 2 cut(s) 6, 65
BmrFI CCNGG 2 cut(s) 242, 340
BmsI GCATC 2 cut(s) 298, 311
BpmI CTGGAG 1 cut(s) 437
BsaHI GRCGYC 1 cut(s) 26
Bse1I ACTGG 1 cut(s) 389
BseBI CCWGG 2 cut(s) 242, 340
BseGI GGATG 2 cut(s) 120, 313
BseMII CTCAG 1 cut(s) 437
BseNI ACTGG 1 cut(s) 389
BseXI GCAGC 2 cut(s) 298, 326
BshFI GGCC 1 cut(s) 64
BsiHKAI GWGCWC 1 cut(s) 146
BsiSI CCGG 1 cut(s) 61
BsmAI GTCTC 2 cut(s) 159, 426
BsmBI CGTCTC 1 cut(s) 159
BsmI GAATGC 2 cut(s) 210, 212
BsnI GGCC 1 cut(s) 64
Bsp1286I GDGCHC 1 cut(s) 146
BspACI CCGC 1 cut(s) 17
BspANI GGCC 1 cut(s) 64
BspCNI CTCAG 1 cut(s) 436
BspLI GGNNCC 2 cut(s) 6, 65
BspMAI CTGCAG 1 cut(s) 316
BspMI ACCTGC 1 cut(s) 469
BsrI ACTGG 1 cut(s) 389
BssNI GRCGYC 1 cut(s) 26
Bst2UI CCWGG 2 cut(s) 242, 340
Bst4CI ACNGT 2 cut(s) 170, 355
BstACI GRCGYC 1 cut(s) 26
BstAPI GCANNNNNTGC 1 cut(s) 308
BstDEI CTNAG 1 cut(s) 423
BstF5I GGATG 2 cut(s) 120, 313
BstMAI GTCTC 2 cut(s) 159, 426
BstMWI GCNNNNNNNGC 2 cut(s) 308, 455
BstNI CCWGG 2 cut(s) 242, 340
BstSCI CCNGG 2 cut(s) 240, 338
BstSFI CTRYAG 1 cut(s) 312
BstV1I GCAGC 2 cut(s) 298, 326
BsuRI GGCC 1 cut(s) 64
BtgZI GCGATG 1 cut(s) 57
BtsCI GGATG 2 cut(s) 120, 313
BveI ACCTGC 1 cut(s) 469
CaiI CAGNNNCTG 1 cut(s) 415
Cfr13I GGNCC 3 cut(s) 43, 63, 481
CseI GACGC 1 cut(s) 15
Csp6I GTAC 1 cut(s) 235
CviAII CATG 1 cut(s) 256
CviJI RGCY 7 cut(s) 5, 64, 136, 317, 331, 449, 458
CviKI_1 RGCY 7 cut(s) 5, 64, 136, 317, 331, 449, 458
CviQI GTAC 1 cut(s) 235
DdeI CTNAG 1 cut(s) 423
Eco47I GGWCC 2 cut(s) 43, 481
EcoRII CCWGG 2 cut(s) 240, 338
EcoT22I ATGCAT 1 cut(s) 212
Esp3I CGTCTC 1 cut(s) 159
FaeI CATG 1 cut(s) 259
FalI AAGNNNNNCTT 2 cut(s) 251, 283
FatI CATG 1 cut(s) 255
FblI GTMKAC 1 cut(s) 29
Fnu4HI GCNGC 2 cut(s) 312, 315
FokI GGATG 2 cut(s) 107, 320
Fsp4HI GCNGC 2 cut(s) 312, 315
GluI GCNGC 2 cut(s) 312, 315
GsuI CTGGAG 1 cut(s) 437
HaeIII GGCC 1 cut(s) 64
HapII CCGG 1 cut(s) 61
HgaI GACGC 1 cut(s) 15
Hin1I GRCGYC 1 cut(s) 26
Hin1II CATG 1 cut(s) 259
HincII GTYRAC 1 cut(s) 351
HindII GTYRAC 1 cut(s) 351
HinfI GANTC 4 cut(s) 48, 149, 245, 419
HpaII CCGG 1 cut(s) 61
HphI GGTGA 2 cut(s) 182, 427
Hpy166II GTNNAC 3 cut(s) 30, 351, 521
Hpy188I TCNGA 4 cut(s) 47, 250, 307, 426
Hpy188III TCNNGA 1 cut(s) 124
Hpy8I GTNNAC 3 cut(s) 30, 351, 521
Hpy99I CGWCG 1 cut(s) 25
HpyAV CCTTC 2 cut(s) 18, 118
HpyCH4III ACNGT 2 cut(s) 170, 355
HpyCH4IV ACGT 2 cut(s) 129, 237
HpyCH4V TGCA 4 cut(s) 194, 210, 314, 359
HpyF10VI GCNNNNNNNGC 2 cut(s) 308, 455
HpyF3I CTNAG 1 cut(s) 423
HpySE526I ACGT 2 cut(s) 129, 237
Hsp92I GRCGYC 1 cut(s) 26
Hsp92II CATG 1 cut(s) 259
LmnI GCTCC 2 cut(s) 10, 328
Lsp1109I GCAGC 2 cut(s) 298, 326
LweI GCATC 2 cut(s) 298, 311
MaeII ACGT 2 cut(s) 129, 237
MboII GAAGA 3 cut(s) 103, 109, 271
MhlI GDGCHC 1 cut(s) 146
MluCI AATT 7 cut(s) 84, 195, 289, 362, 376, 403, 427
MlyI GAGTC 2 cut(s) 143, 428
MnlI CCTC 3 cut(s) 79, 87, 225
Mph1103I ATGCAT 1 cut(s) 212
MroXI GAANNNNTTC 2 cut(s) 210, 263
MseI TTAA 1 cut(s) 200
MspI CCGG 1 cut(s) 61
MspR9I CCNGG 2 cut(s) 242, 340
Mva1269I GAATGC 2 cut(s) 210, 212
MvaI CCWGG 2 cut(s) 242, 340
MwoI GCNNNNNNNGC 2 cut(s) 308, 455
NlaIII CATG 1 cut(s) 259
NlaIV GGNNCC 2 cut(s) 6, 65
NsiI ATGCAT 1 cut(s) 212
PcsI WCGNNNNNNNCGW 2 cut(s) 17, 43
PctI GAATGC 2 cut(s) 210, 212
PdmI GAANNNNTTC 2 cut(s) 210, 263
PfeI GAWTC 2 cut(s) 48, 245
PflFI GACNNNGTC 1 cut(s) 25
PfoI TCCNGGA 1 cut(s) 240
PkrI GCNGC 2 cut(s) 313, 316
PleI GAGTC 2 cut(s) 143, 427
PpsI GAGTC 2 cut(s) 143, 427
Psp6I CCWGG 2 cut(s) 240, 338
PspGI CCWGG 2 cut(s) 240, 338
PspN4I GGNNCC 2 cut(s) 6, 65
PspPI GGNCC 3 cut(s) 43, 63, 481
PstI CTGCAG 1 cut(s) 316
PstNI CAGNNNCTG 1 cut(s) 415
PsyI GACNNNGTC 1 cut(s) 25
RsaI GTAC 1 cut(s) 236
RsaNI GTAC 1 cut(s) 235
SaqAI TTAA 1 cut(s) 200
SatI GCNGC 2 cut(s) 312, 315
Sau96I GGNCC 3 cut(s) 43, 63, 481
SchI GAGTC 2 cut(s) 143, 428
ScrFI CCNGG 2 cut(s) 242, 340
SduI GDGCHC 1 cut(s) 146
SetI ASST 7 cut(s) 132, 225, 236, 240, 333, 483, 510
SfaNI GCATC 2 cut(s) 298, 311
SfcI CTRYAG 1 cut(s) 312
SinI GGWCC 2 cut(s) 43, 481
Sse9I AATT 7 cut(s) 84, 195, 289, 362, 376, 403, 427
SsiI CCGC 1 cut(s) 17
StyD4I CCNGG 2 cut(s) 240, 338
TaaI ACNGT 2 cut(s) 170, 355
TaiI ACGT 2 cut(s) 132, 240
TaqI TCGA 2 cut(s) 11, 93
TasI AATT 7 cut(s) 84, 195, 289, 362, 376, 403, 427
TfiI GAWTC 2 cut(s) 48, 245
Tru1I TTAA 1 cut(s) 200
Tru9I TTAA 1 cut(s) 200
TseI GCWGC 2 cut(s) 311, 314
TspDTI ATGAA 4 cut(s) 103, 109, 272, 488
TspGWI ACGGA 1 cut(s) 69
Tth111I GACNNNGTC 1 cut(s) 25
VpaK11BI GGWCC 2 cut(s) 43, 481
XapI RAATTY 1 cut(s) 289
XmiI GTMKAC 1 cut(s) 29
XmnI GAANNNNTTC 2 cut(s) 210, 263
Zsp2I ATGCAT 1 cut(s) 212
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.