RchiOBHm_Chr5g0048961

callose synthase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
5
OG0020564
Physical Location & Seq
Forward (+)
46273896 .. 46275917
2022 bp
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UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 543 bp
ATGGGTATCAGCCTATCAGGGGAAGAAGGTAACACTTGCAGTCCACTTCTTTGTTTACCACCTGGAATCCTGGATATAGCTCACCGCAACAAAGACTACTGGTTTATGGACTTTCTTGGGCTCTTGTGGTATCTGTCCTTTCTCATCTTTAAGAGACGAAGATTTTACACCTACTTTCAGCTCGAGTTCAGGATATTGAAGTTGCTTCTTTTTCTTGGCTACATGTCAATCATGAGCGTCTTGTTTGTATTTTGTGATCTCACTATTTCAGATCTATTTGCTGCTATGCTTGCTTTCTTTCCCACAGGGTGGGCCCCTCTTCAGATAGGACAAGTATGCAGCGTGATGTTCAGGAGCCAAGGATTCTCAGAATCGATAAATGAGTTTGGAAGAGCATACGATTATATAACGGGAGTGATAATCTTCATGCCCATTGCCATTTGTCATGGTTCCTATTTGTATCAAAATGCCAAACACGCTTGCTCTTTAACCAAGCATCAAGTAGAGGCCTATAAAGGTTCAATGGTTCTTGCAGGGAGGTAG

Protein Analysis

180

Amino Acids

20.62

Weight (kDa)

8.21

Isoelectric Point (pI)

49.38

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0020564)

Species Orthologous Gene IDs
rosa_chinensis RchiOBHm_Chr5g0048961
rosa_laevigata RLG00000034571
rosa_multiflora Rmu_sc0007946.1_g000002
rosa_rugosa Rorug05G0241400
rosa_samantha Rh5AG321500
rosa_wichuraiana Rw5G030340

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 309
AciI CCGC 1 cut(s) 85
AcuI CTGAAG 1 cut(s) 305
AdeI CACNNNGTG 1 cut(s) 309
AfiI CCNNNNNNNGG 2 cut(s) 19, 309
AflIII ACRYGT 1 cut(s) 222
AgsI TTSAA 2 cut(s) 199, 522
AjnI CCWGG 2 cut(s) 61, 69
AluBI AGCT 2 cut(s) 80, 181
AluI AGCT 2 cut(s) 80, 181
Alw26I GTCTC 1 cut(s) 148
Ama87I CYCGRG 1 cut(s) 182
AoxI GGCC 2 cut(s) 312, 507
ApaI GGGCCC 1 cut(s) 316
ApeKI GCWGC 2 cut(s) 281, 339
AspS9I GGNCC 2 cut(s) 312, 313
AsuHPI GGTGA 1 cut(s) 74
AvaI CYCGRG 1 cut(s) 182
BaeGI GKGCMC 1 cut(s) 316
BanII GRGCYC 2 cut(s) 123, 316
BbvI GCAGC 2 cut(s) 268, 351
BciT130I CCWGG 2 cut(s) 63, 71
BcoDI GTCTC 1 cut(s) 148
BglII AGATCT 1 cut(s) 271
BisI GCNGC 2 cut(s) 282, 340
BlsI GCNGC 2 cut(s) 283, 341
Bme1390I CCNGG 2 cut(s) 63, 71
BmeT110I CYCGRG 1 cut(s) 182
BmgT120I GGNCC 2 cut(s) 312, 313
BmiI GGNNCC 4 cut(s) 314, 315, 356, 451
BmrFI CCNGG 2 cut(s) 63, 71
BmsI GCATC 1 cut(s) 505
Bsa29I ATCGAT 1 cut(s) 374
BsaJI CCNNGG 1 cut(s) 358
Bsc4I CCNNNNNNNGG 2 cut(s) 19, 309
Bse1I ACTGG 1 cut(s) 104
Bse3DI GCAATG 1 cut(s) 432
BseBI CCWGG 2 cut(s) 63, 71
BseCI ATCGAT 1 cut(s) 374
BseDI CCNNGG 1 cut(s) 358
BseLI CCNNNNNNNGG 2 cut(s) 19, 309
BseMI GCAATG 1 cut(s) 432
BseMII CTCAG 1 cut(s) 381
BseNI ACTGG 1 cut(s) 104
BseSI GKGCMC 1 cut(s) 316
BseXI GCAGC 2 cut(s) 268, 351
BshFI GGCC 2 cut(s) 314, 509
BshVI ATCGAT 1 cut(s) 374
BsiHKCI CYCGRG 1 cut(s) 182
BslI CCNNNNNNNGG 2 cut(s) 19, 309
BsmAI GTCTC 1 cut(s) 148
BsmBI CGTCTC 1 cut(s) 148
BsnI GGCC 2 cut(s) 314, 509
BsoBI CYCGRG 1 cut(s) 182
Bsp120I GGGCCC 1 cut(s) 312
Bsp1286I GDGCHC 2 cut(s) 123, 316
Bsp143I GATC 2 cut(s) 256, 271
BspACI CCGC 1 cut(s) 85
BspANI GGCC 2 cut(s) 314, 509
BspCNI CTCAG 1 cut(s) 380
BspDI ATCGAT 1 cut(s) 374
BspHI TCATGA 1 cut(s) 231
BspLI GGNNCC 4 cut(s) 314, 315, 356, 451
BspQI GCTCTTC 1 cut(s) 385
BsrDI GCAATG 1 cut(s) 432
BsrI ACTGG 1 cut(s) 104
BssECI CCNNGG 1 cut(s) 358
BssMI GATC 2 cut(s) 256, 271
BssT1I CCWWGG 1 cut(s) 358
Bst2UI CCWGG 2 cut(s) 63, 71
Bst6I CTCTTC 2 cut(s) 324, 385
BstC8I GCNNGC 2 cut(s) 291, 481
BstDEI CTNAG 1 cut(s) 367
BstKTI GATC 2 cut(s) 259, 274
BstMAI GTCTC 1 cut(s) 148
BstMBI GATC 2 cut(s) 256, 271
BstMWI GCNNNNNNNGC 2 cut(s) 290, 476
BstNI CCWGG 2 cut(s) 63, 71
BstNSI RCATGY 1 cut(s) 226
BstSCI CCNGG 2 cut(s) 61, 69
BstSLI GKGCMC 1 cut(s) 316
BstV1I GCAGC 2 cut(s) 268, 351
BstX2I RGATCY 1 cut(s) 271
BstYI RGATCY 1 cut(s) 271
Bsu15I ATCGAT 1 cut(s) 374
BsuRI GGCC 2 cut(s) 314, 509
BsuTUI ATCGAT 1 cut(s) 374
Cac8I GCNNGC 2 cut(s) 291, 481
CciI TCATGA 1 cut(s) 231
Cfr13I GGNCC 2 cut(s) 312, 313
ClaI ATCGAT 1 cut(s) 374
CseI GACGC 1 cut(s) 226
CviAII CATG 4 cut(s) 223, 232, 427, 446
CviJI RGCY 8 cut(s) 12, 80, 121, 181, 219, 314, 357, 509
CviKI_1 RGCY 8 cut(s) 12, 80, 121, 181, 219, 314, 357, 509
DdeI CTNAG 1 cut(s) 367
DpnI GATC 2 cut(s) 258, 273
DpnII GATC 2 cut(s) 256, 271
DraIII CACNNNGTG 1 cut(s) 309
Eam1104I CTCTTC 2 cut(s) 324, 385
EarI CTCTTC 2 cut(s) 324, 385
Eco130I CCWWGG 1 cut(s) 358
Eco147I AGGCCT 1 cut(s) 509
Eco24I GRGCYC 2 cut(s) 123, 316
Eco57I CTGAAG 1 cut(s) 305
Eco88I CYCGRG 1 cut(s) 182
EcoO109I RGGNCCY 1 cut(s) 313
EcoRII CCWGG 2 cut(s) 61, 69
EcoT14I CCWWGG 1 cut(s) 358
EcoT38I GRGCYC 2 cut(s) 123, 316
ErhI CCWWGG 1 cut(s) 358
Esp3I CGTCTC 1 cut(s) 148
FaeI CATG 4 cut(s) 226, 235, 430, 449
FatI CATG 4 cut(s) 222, 231, 426, 445
Fnu4HI GCNGC 2 cut(s) 282, 340
FriOI GRGCYC 2 cut(s) 123, 316
Fsp4HI GCNGC 2 cut(s) 282, 340
GluI GCNGC 2 cut(s) 282, 340
HaeIII GGCC 2 cut(s) 314, 509
HgaI GACGC 1 cut(s) 226
Hin1II CATG 4 cut(s) 226, 235, 430, 449
HinfI GANTC 3 cut(s) 66, 363, 371
HphI GGTGA 1 cut(s) 74
Hpy166II GTNNAC 2 cut(s) 44, 56
Hpy188I TCNGA 3 cut(s) 271, 324, 370
Hpy188III TCNNGA 3 cut(s) 190, 232, 352
Hpy8I GTNNAC 2 cut(s) 44, 56
HpyAV CCTTC 1 cut(s) 20
HpyCH4V TGCA 3 cut(s) 39, 339, 533
HpyF10VI GCNNNNNNNGC 2 cut(s) 290, 476
HpyF3I CTNAG 1 cut(s) 367
Hsp92II CATG 4 cut(s) 226, 235, 430, 449
Kzo9I GATC 2 cut(s) 256, 271
LguI GCTCTTC 1 cut(s) 385
LmnI GCTCC 1 cut(s) 354
Lsp1109I GCAGC 2 cut(s) 268, 351
LweI GCATC 1 cut(s) 505
MaeIII GTNAC 1 cut(s) 29
MalI GATC 2 cut(s) 258, 273
MboI GATC 2 cut(s) 256, 271
MboII GAAGA 5 cut(s) 35, 171, 311, 402, 415
MflI RGATCY 1 cut(s) 271
MhlI GDGCHC 2 cut(s) 123, 316
MnlI CCTC 3 cut(s) 327, 499, 531
MseI TTAA 2 cut(s) 150, 488
MspR9I CCNGG 2 cut(s) 63, 71
MvaI CCWGG 2 cut(s) 63, 71
MwoI GCNNNNNNNGC 2 cut(s) 290, 476
NdeII GATC 2 cut(s) 256, 271
NlaIII CATG 4 cut(s) 226, 235, 430, 449
NlaIV GGNNCC 4 cut(s) 314, 315, 356, 451
NspI RCATGY 1 cut(s) 226
PaeR7I CTCGAG 1 cut(s) 182
PagI TCATGA 1 cut(s) 231
PceI AGGCCT 1 cut(s) 509
PciI ACATGT 1 cut(s) 222
PciSI GCTCTTC 1 cut(s) 385
PfeI GAWTC 3 cut(s) 66, 363, 371
PflMI CCANNNNNTGG 1 cut(s) 309
PfoI TCCNGGA 1 cut(s) 69
PkrI GCNGC 2 cut(s) 283, 341
PscI ACATGT 1 cut(s) 222
Psp6I CCWGG 2 cut(s) 61, 69
PspGI CCWGG 2 cut(s) 61, 69
PspN4I GGNNCC 4 cut(s) 314, 315, 356, 451
PspOMI GGGCCC 1 cut(s) 312
PspPI GGNCC 2 cut(s) 312, 313
PspXI VCTCGAGB 1 cut(s) 182
PsuI RGATCY 1 cut(s) 271
SapI GCTCTTC 1 cut(s) 385
SaqAI TTAA 2 cut(s) 150, 488
SatI GCNGC 2 cut(s) 282, 340
Sau3AI GATC 2 cut(s) 256, 271
Sau96I GGNCC 2 cut(s) 312, 313
ScrFI CCNGG 2 cut(s) 63, 71
SduI GDGCHC 2 cut(s) 123, 316
SetI ASST 7 cut(s) 31, 64, 82, 173, 183, 520, 542
SfaNI GCATC 1 cut(s) 505
Sfr274I CTCGAG 1 cut(s) 182
SlaI CTCGAG 1 cut(s) 182
SmlI CTYRAG 1 cut(s) 182
SmoI CTYRAG 1 cut(s) 182
SseBI AGGCCT 1 cut(s) 509
SsiI CCGC 1 cut(s) 85
StuI AGGCCT 1 cut(s) 509
StyD4I CCNGG 2 cut(s) 61, 69
StyI CCWWGG 1 cut(s) 358
TaqI TCGA 2 cut(s) 183, 374
TfiI GAWTC 3 cut(s) 66, 363, 371
Tru1I TTAA 2 cut(s) 150, 488
Tru9I TTAA 2 cut(s) 150, 488
TseI GCWGC 2 cut(s) 281, 339
TspDTI ATGAA 1 cut(s) 415
Van91I CCANNNNNTGG 1 cut(s) 309
XceI RCATGY 1 cut(s) 226
XhoI CTCGAG 1 cut(s) 182
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.