Rw5G030340

callose synthase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr5
OG0020564
Physical Location & Seq
Forward (+)
48013591 .. 48015445
1855 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw5G030340.1

Sequence Viewer

Length: 636 bp
ATGATTGGATGGATTGGAAGAGGTGGATGGGAATTGATGTCTGACCGGAAGAAAGTTGGGAATCATGGTGGGATGAAGAATAGGAACACCTCAAACACATGGGTATCAGGGGAAGAAGGTAACACTTGCAGTCCACTTTGTTTACCACCTGGAATCCTGGATATAGCTCACCACAACAAAGACTACTGGTTTATGGACCTTCTTGGGCTGTTGTGGTATCTGTCCTTTCTCATCTTTAAGATGGTTTCTATGCGGAGACGAAGATTTTACACCAACTTTCAGCTCAAGTTCAGGATACTGAAATTGCTTCTTTTTCTTGGCTACATGTCAATCATGAGCGTCTTGTTTGTATTTTGTGATCTCACTATTTCAGATCTATTTGCTGCTATGCTTTCTTTCTTTCCCACAGGGTGGGCCCCTCTTCAAATAGGACAAGTATGCAGCGTGATGTTCAGGAGCCAAGGATTCTCAGAATCGATAAATGAGTTTGGAAGAGCATACGATTATATAATGGGAGTGATATCTTCATGCCCATTGCCATTTGTCATGGTTCCTATTTGTGCCAAACACGGTTGCTCTTTAACCAAGCATCAAGTAGAGGCCTATAAAGGTCCAATGGTTCTTGCAGGGAAGTAG

Protein Analysis

211

Amino Acids

24.0

Weight (kDa)

9.23

Isoelectric Point (pI)

46.09

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0020564)

Species Orthologous Gene IDs
rosa_chinensis RchiOBHm_Chr5g0048961
rosa_laevigata RLG00000034571
rosa_multiflora Rmu_sc0007946.1_g000002
rosa_rugosa Rorug05G0241400
rosa_samantha Rh5AG321500
rosa_wichuraiana Rw5G030340

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 411
AciI CCGC 1 cut(s) 253
AdeI CACNNNGTG 1 cut(s) 411
AfiI CCNNNNNNNGG 1 cut(s) 411
AflIII ACRYGT 1 cut(s) 324
AgsI TTSAA 1 cut(s) 425
AjnI CCWGG 2 cut(s) 148, 156
AluBI AGCT 2 cut(s) 167, 283
AluI AGCT 2 cut(s) 167, 283
Alw26I GTCTC 1 cut(s) 250
AoxI GGCC 2 cut(s) 414, 600
ApaI GGGCCC 1 cut(s) 418
ApeKI GCWGC 2 cut(s) 383, 441
AspS9I GGNCC 4 cut(s) 196, 414, 415, 611
AsuHPI GGTGA 1 cut(s) 161
AvaII GGWCC 2 cut(s) 196, 611
BaeGI GKGCMC 1 cut(s) 418
BaeI ACNNNNGTAYC 2 cut(s) 87, 120
BanII GRGCYC 1 cut(s) 418
BbvI GCAGC 2 cut(s) 370, 453
BccI CCATC 3 cut(s) 3, 21, 235
BciT130I CCWGG 2 cut(s) 150, 158
BciVI GTATCC 1 cut(s) 288
BcoDI GTCTC 1 cut(s) 250
BfuI GTATCC 1 cut(s) 288
BglII AGATCT 1 cut(s) 373
BisI GCNGC 2 cut(s) 384, 442
BlsI GCNGC 2 cut(s) 385, 443
Bme1390I CCNGG 2 cut(s) 150, 158
Bme18I GGWCC 2 cut(s) 196, 611
BmgT120I GGNCC 4 cut(s) 196, 414, 415, 611
BmiI GGNNCC 4 cut(s) 416, 417, 458, 552
BmrFI CCNGG 2 cut(s) 150, 158
BmsI GCATC 1 cut(s) 598
BpuEI CTTGAG 1 cut(s) 269
Bsa29I ATCGAT 1 cut(s) 476
BsaJI CCNNGG 1 cut(s) 460
BsaWI WCCGGW 1 cut(s) 45
Bsc4I CCNNNNNNNGG 1 cut(s) 411
Bse1I ACTGG 1 cut(s) 191
Bse3DI GCAATG 1 cut(s) 533
BseBI CCWGG 2 cut(s) 150, 158
BseCI ATCGAT 1 cut(s) 476
BseDI CCNNGG 1 cut(s) 460
BseGI GGATG 3 cut(s) 14, 32, 78
BseLI CCNNNNNNNGG 1 cut(s) 411
BseMI GCAATG 1 cut(s) 533
BseMII CTCAG 1 cut(s) 483
BseNI ACTGG 1 cut(s) 191
BseSI GKGCMC 1 cut(s) 418
BseXI GCAGC 2 cut(s) 370, 453
BshFI GGCC 2 cut(s) 416, 602
BshVI ATCGAT 1 cut(s) 476
BsiSI CCGG 1 cut(s) 46
BslI CCNNNNNNNGG 1 cut(s) 411
BsmAI GTCTC 1 cut(s) 250
BsmBI CGTCTC 1 cut(s) 250
BsnI GGCC 2 cut(s) 416, 602
Bsp120I GGGCCC 1 cut(s) 414
Bsp1286I GDGCHC 1 cut(s) 418
Bsp143I GATC 2 cut(s) 358, 373
BspACI CCGC 1 cut(s) 253
BspANI GGCC 2 cut(s) 416, 602
BspCNI CTCAG 1 cut(s) 482
BspDI ATCGAT 1 cut(s) 476
BspHI TCATGA 1 cut(s) 333
BspLI GGNNCC 4 cut(s) 416, 417, 458, 552
BspQI GCTCTTC 1 cut(s) 487
BsrDI GCAATG 1 cut(s) 533
BsrI ACTGG 1 cut(s) 191
BssECI CCNNGG 1 cut(s) 460
BssMI GATC 2 cut(s) 358, 373
BssT1I CCWWGG 1 cut(s) 460
Bst2UI CCWGG 2 cut(s) 150, 158
Bst4CI ACNGT 1 cut(s) 572
Bst6I CTCTTC 3 cut(s) 13, 426, 487
BstDEI CTNAG 1 cut(s) 469
BstF5I GGATG 3 cut(s) 14, 32, 78
BstKTI GATC 2 cut(s) 361, 376
BstMAI GTCTC 1 cut(s) 250
BstMBI GATC 2 cut(s) 358, 373
BstNI CCWGG 2 cut(s) 150, 158
BstNSI RCATGY 1 cut(s) 328
BstSCI CCNGG 2 cut(s) 148, 156
BstSLI GKGCMC 1 cut(s) 418
BstV1I GCAGC 2 cut(s) 370, 453
BstX2I RGATCY 1 cut(s) 373
BstYI RGATCY 1 cut(s) 373
Bsu15I ATCGAT 1 cut(s) 476
BsuI GTATCC 1 cut(s) 288
BsuRI GGCC 2 cut(s) 416, 602
BsuTUI ATCGAT 1 cut(s) 476
BtsCI GGATG 3 cut(s) 14, 32, 78
CciI TCATGA 1 cut(s) 333
Cfr13I GGNCC 4 cut(s) 196, 414, 415, 611
ClaI ATCGAT 1 cut(s) 476
CseI GACGC 1 cut(s) 328
CviAII CATG 6 cut(s) 65, 99, 325, 334, 528, 547
CviJI RGCY 7 cut(s) 167, 208, 283, 321, 416, 459, 602
CviKI_1 RGCY 7 cut(s) 167, 208, 283, 321, 416, 459, 602
DdeI CTNAG 1 cut(s) 469
DpnI GATC 2 cut(s) 360, 375
DpnII GATC 2 cut(s) 358, 373
DraIII CACNNNGTG 1 cut(s) 411
Eam1104I CTCTTC 3 cut(s) 13, 426, 487
EarI CTCTTC 3 cut(s) 13, 426, 487
Eco130I CCWWGG 1 cut(s) 460
Eco147I AGGCCT 1 cut(s) 602
Eco24I GRGCYC 1 cut(s) 418
Eco32I GATATC 1 cut(s) 522
Eco47I GGWCC 2 cut(s) 196, 611
EcoO109I RGGNCCY 1 cut(s) 415
EcoRII CCWGG 2 cut(s) 148, 156
EcoRV GATATC 1 cut(s) 522
EcoT14I CCWWGG 1 cut(s) 460
EcoT38I GRGCYC 1 cut(s) 418
ErhI CCWWGG 1 cut(s) 460
Esp3I CGTCTC 1 cut(s) 250
FaeI CATG 6 cut(s) 68, 102, 328, 337, 531, 550
FatI CATG 6 cut(s) 64, 98, 324, 333, 527, 546
Fnu4HI GCNGC 2 cut(s) 384, 442
FokI GGATG 3 cut(s) 21, 39, 85
FriOI GRGCYC 1 cut(s) 418
Fsp4HI GCNGC 2 cut(s) 384, 442
GluI GCNGC 2 cut(s) 384, 442
HaeIII GGCC 2 cut(s) 416, 602
HapII CCGG 1 cut(s) 46
HgaI GACGC 1 cut(s) 328
Hin1II CATG 6 cut(s) 68, 102, 328, 337, 531, 550
HinfI GANTC 4 cut(s) 61, 153, 465, 473
HpaII CCGG 1 cut(s) 46
HphI GGTGA 1 cut(s) 161
Hpy166II GTNNAC 2 cut(s) 134, 143
Hpy188I TCNGA 3 cut(s) 43, 373, 472
Hpy188III TCNNGA 3 cut(s) 292, 334, 454
Hpy8I GTNNAC 2 cut(s) 134, 143
HpyAV CCTTC 2 cut(s) 110, 209
HpyCH4III ACNGT 1 cut(s) 572
HpyCH4V TGCA 3 cut(s) 129, 441, 626
HpyF3I CTNAG 1 cut(s) 469
Hsp92II CATG 6 cut(s) 68, 102, 328, 337, 531, 550
Kzo9I GATC 2 cut(s) 358, 373
LguI GCTCTTC 1 cut(s) 487
LmnI GCTCC 1 cut(s) 456
Lsp1109I GCAGC 2 cut(s) 370, 453
LweI GCATC 1 cut(s) 598
MaeIII GTNAC 1 cut(s) 119
MalI GATC 2 cut(s) 360, 375
MboI GATC 2 cut(s) 358, 373
MboII GAAGA 8 cut(s) 30, 61, 88, 125, 273, 413, 504, 516
MflI RGATCY 1 cut(s) 373
MhlI GDGCHC 1 cut(s) 418
MluCI AATT 2 cut(s) 32, 302
MnlI CCTC 4 cut(s) 14, 100, 429, 592
MseI TTAA 2 cut(s) 237, 581
MspI CCGG 1 cut(s) 46
MspR9I CCNGG 2 cut(s) 150, 158
MvaI CCWGG 2 cut(s) 150, 158
NdeII GATC 2 cut(s) 358, 373
NlaIII CATG 6 cut(s) 68, 102, 328, 337, 531, 550
NlaIV GGNNCC 4 cut(s) 416, 417, 458, 552
NspI RCATGY 1 cut(s) 328
PagI TCATGA 1 cut(s) 333
PceI AGGCCT 1 cut(s) 602
PciI ACATGT 1 cut(s) 324
PciSI GCTCTTC 1 cut(s) 487
PfeI GAWTC 4 cut(s) 61, 153, 465, 473
PflMI CCANNNNNTGG 1 cut(s) 411
PfoI TCCNGGA 1 cut(s) 156
PkrI GCNGC 2 cut(s) 385, 443
PscI ACATGT 1 cut(s) 324
Psp6I CCWGG 2 cut(s) 148, 156
PspGI CCWGG 2 cut(s) 148, 156
PspN4I GGNNCC 4 cut(s) 416, 417, 458, 552
PspOMI GGGCCC 1 cut(s) 414
PspPI GGNCC 4 cut(s) 196, 414, 415, 611
PsuI RGATCY 1 cut(s) 373
SapI GCTCTTC 1 cut(s) 487
SaqAI TTAA 2 cut(s) 237, 581
SatI GCNGC 2 cut(s) 384, 442
Sau3AI GATC 2 cut(s) 358, 373
Sau96I GGNCC 4 cut(s) 196, 414, 415, 611
ScrFI CCNGG 2 cut(s) 150, 158
SduI GDGCHC 1 cut(s) 418
SetI ASST 8 cut(s) 25, 92, 121, 151, 169, 201, 285, 613
SfaNI GCATC 1 cut(s) 598
SinI GGWCC 2 cut(s) 196, 611
SmlI CTYRAG 1 cut(s) 284
SmoI CTYRAG 1 cut(s) 284
Sse9I AATT 2 cut(s) 32, 302
SseBI AGGCCT 1 cut(s) 602
SsiI CCGC 1 cut(s) 253
StuI AGGCCT 1 cut(s) 602
StyD4I CCNGG 2 cut(s) 148, 156
StyI CCWWGG 1 cut(s) 460
TaaI ACNGT 1 cut(s) 572
TaqI TCGA 1 cut(s) 476
TasI AATT 2 cut(s) 32, 302
TfiI GAWTC 4 cut(s) 61, 153, 465, 473
Tru1I TTAA 2 cut(s) 237, 581
Tru9I TTAA 2 cut(s) 237, 581
TseI GCWGC 2 cut(s) 383, 441
TspDTI ATGAA 2 cut(s) 89, 516
Van91I CCANNNNNTGG 1 cut(s) 411
VpaK11BI GGWCC 2 cut(s) 196, 611
XceI RCATGY 1 cut(s) 328
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.