RchiOBHm_Chr5g0057471

alpha-L-fucosidase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
5
OG0015991
Physical Location & Seq
Reverse (-)
61368918 .. 61372422
3505 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1593 bp
ATGAACCACACCCACACCCCAATCCCCATTTCCATTTCAAGATTCCACCTTTTACTCCTTCTCTCACTTTTACTCTCACTCTCTTCTTCTTCTTCTTCTTCCTCTGCTTCTTCATCCTCATTGCTCAAAAAGCCACCCCCTCTTCCAATTCTACCCCTCCCCTCAGCTCCCCAGCTCCAATGGCAACTCGGCCACATGGCCATGTTCCTCCACTTCGGACCCAACACCTTCACCGACTCCGAATGGGGCACCGGCCACGCTGACCCCTCCATCTTCAACCCCAAAAACCTCAACGCCACCCAGTGGGTCAAAGTCGCCAAAGACTCTGGCTTTTCGCGCGTGATCCTCACCGCCAAACACCACGATGGCTTCTGCTTGTGGCCCACCGAGTACACCAACTACTCGGTCAGCTCCAGCCCGTGGAGGAGCGGCGGCGGTGACGTGGTGGGTGAGCTCGCCGAGGCTGCTAGGGACGCCGGTATTGGATTGGGGCTTTACCTTTCGCCTTGGGATCGGCACGAGCCGTGTTATGGGAAGACTTTGGAGTACAATGAATTCTATATGGCCCAAATGACAGAGCTCCTCACAAGGTATGGAGATATCAAGGAAGTATGGTTGGATGGTGCAAAAGGGGAAGGGGAGAAGGATATGGATTATTTCTTTGATTCTTGGTTCAGTCTGATTCATCAACACCAGCCAGGCGCTGTCATTTTCTCTGATGCTGGTCCCGACACCAGGTGGGTTGGCGACGAAGCTGGTGTTGCTGGGTCTACTTGTTGGTCTCTTTTCAATAGGAGTTCTGCCAAGATTGGTGACACTGATTTCGAATATTCAAGAGGAGGTGACCCACTTGGTCATGATTGGGTACCAGCTGAGTGTGACGTCTCAATTCGTCCTGGTTGGTTTTGGCATCCATCAGAAGTTCCAAAATCTGCAAGGACTCTGCTTGAACTATACTACAAATCAGTTGGTAGGAACTGTCTCTTGTTACTAAATGTACCCCCAAACTCTTCTGGTCTCATATCAGCAGAAGACATTCAAGTGCTTAAAGAATTCAGTGAGCTCCGAAGATCTATATTCTCACAGAATCTAGCTATGGATGCCATTATTCGTGCGAGCAGTACACGAGGAAGCACCAATAATTCTTGGTTCAATGCCTATAATGTCATAGAAGAAGGTTTTCATTCCTATTGGGCACCTGATGAGAATAAGTCTATTTGGGTTTTATGCTTAGATCTTCAAGAACTAGTATCATTCAACATTCTTCAAGTTGAAGAGCCAATTCACATGGGACAGCGGGTTATTGAGTTCCATCTTGAGATCTTGAATGCAGGTGGCAAGTGGAATAAAGTAACTAATGGAACCACTGTGGGATATCGGAGGCTTTTGCAATTTCCAACAGTTAAGTCTCGAAACTTGAGGTTTGTTATTGACAAGTCCCGGGCAGACCCACTGATATCCTATCTGGGTATATATATGGATCCATTCTCCATTTTAAGCAACATATCTCATACAACCACACAGACAGGCATCAATAGCAGTCAAATAATTCACCATATTGAATTCAACCATTCTCAAATTGCTGTATTGTAG

Protein Analysis

530

Amino Acids

59.3

Weight (kDa)

5.78

Isoelectric Point (pI)

48.25

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Alpha_L_fucos PF01120 90 - 353 2.7e-35 Alpha-L-fucosidase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0015991)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G28100
fragaria_vesca FvH4_3g30940
malus_domestica MD00G1032500.v1.1
prunus_persica Prupe.6G109300_v2.0.a1
rosa_chinensis RchiOBHm_Chr5g0057471
rosa_laevigata RLG00000035158
rosa_multiflora Rmu_sc0000508.1_g000012 Rmu_sc0003730.1_g000020
rosa_roxburghii Rroxscaffold_1G00022980
rosa_rugosa Rorug05G0307500
rosa_samantha Rh5AG376100 Rh5BG387000 Rh5CG410400 Rh5DG401200
rosa_wichuraiana Rw5G035510

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 1322
AatII GACGTC 1 cut(s) 885
Acc36I ACCTGC 1 cut(s) 1322
Acc65I GGTACC 1 cut(s) 865
AccB1I GGYRCC 3 cut(s) 248, 865, 1195
AccB7I CCANNNNNTGG 2 cut(s) 303, 420
AccBSI CCGCTC 1 cut(s) 429
AccI GTMKAC 1 cut(s) 770
AccII CGCG 2 cut(s) 337, 339
AciI CCGC 5 cut(s) 351, 429, 432, 435, 1297
AclWI GGATC 4 cut(s) 337, 519, 1475, 1488
AcoI YGGCCR 3 cut(s) 190, 198, 253
AcsI RAATTY 3 cut(s) 554, 1052, 1562
AcyI GRCGYC 2 cut(s) 474, 882
AdeI CACNNNGTG 2 cut(s) 303, 738
AfaI GTAC 5 cut(s) 392, 548, 867, 999, 1123
AfiI CCNNNNNNNGG 4 cut(s) 303, 420, 530, 735
AhlI ACTAGT 1 cut(s) 1246
AjiI CACGTC 1 cut(s) 442
AjnI CCWGG 3 cut(s) 697, 734, 895
AjuI GAANNNNNNNTTGG 2 cut(s) 13, 45
AluBI AGCT 9 cut(s) 167, 175, 411, 454, 580, 755, 872, 1063, 1094
AluI AGCT 9 cut(s) 167, 175, 411, 454, 580, 755, 872, 1063, 1094
Alw21I GWGCWC 3 cut(s) 456, 582, 1065
Alw26I GTCTC 5 cut(s) 786, 889, 986, 1022, 1413
AlwI GGATC 4 cut(s) 337, 519, 1475, 1488
AlwNI CAGNNNCTG 1 cut(s) 704
Ama87I CYCGRG 1 cut(s) 1440
AoxI GGCC 5 cut(s) 190, 198, 253, 380, 564
ApeKI GCWGC 1 cut(s) 464
ApoI RAATTY 3 cut(s) 554, 1052, 1562
ArsI GACNNNNNNTTYG 2 cut(s) 806, 838
Asp700I GAANNNNTTC 2 cut(s) 1035, 1179
Asp718I GGTACC 1 cut(s) 865
AspLEI GCGC 2 cut(s) 339, 704
AspS9I GGNCC 4 cut(s) 218, 381, 565, 725
AsuC2I CCSGG 2 cut(s) 1441, 1442
AsuHPI GGTGA 7 cut(s) 223, 340, 449, 461, 824, 854, 1544
AsuII TTCGAA 1 cut(s) 825
AvaI CYCGRG 1 cut(s) 1440
AvaII GGWCC 2 cut(s) 218, 725
BaeGI GKGCMC 2 cut(s) 251, 1198
BalI TGGCCA 1 cut(s) 200
BamHI GGATCC 1 cut(s) 1480
BanI GGYRCC 3 cut(s) 248, 865, 1195
BanII GRGCYC 3 cut(s) 456, 582, 1065
BauI CACGAG 2 cut(s) 518, 1125
BbsI GAAGAC 2 cut(s) 542, 1038
Bbv12I GWGCWC 3 cut(s) 456, 582, 1065
BbvCI CCTCAGC 1 cut(s) 163
BbvI GCAGC 1 cut(s) 451
BccI CCATC 5 cut(s) 278, 359, 614, 922, 1320
BceAI ACGGC 1 cut(s) 508
BciT130I CCWGG 3 cut(s) 699, 736, 897
BcnI CCSGG 2 cut(s) 1441, 1442
BcoDI GTCTC 5 cut(s) 786, 889, 986, 1022, 1413
BcuI ACTAGT 1 cut(s) 1246
BfaI CTAG 3 cut(s) 468, 1091, 1247
BfoI RGCGCY 1 cut(s) 705
BfuAI ACCTGC 1 cut(s) 1322
BglII AGATCT 3 cut(s) 1070, 1234, 1320
BisI GCNGC 3 cut(s) 430, 433, 465
BlsI GCNGC 3 cut(s) 431, 434, 466
Bme1390I CCNGG 5 cut(s) 699, 736, 897, 1441, 1442
Bme18I GGWCC 2 cut(s) 218, 725
BmeT110I CYCGRG 1 cut(s) 1440
BmgBI CACGTC 1 cut(s) 442
BmgT120I GGNCC 4 cut(s) 218, 381, 565, 725
BmiI GGNNCC 7 cut(s) 220, 250, 727, 867, 1197, 1363, 1482
BmrFI CCNGG 5 cut(s) 699, 736, 897, 1441, 1442
BmrI ACTGGG 1 cut(s) 295
BmsI GCATC 4 cut(s) 709, 919, 1090, 1539
BmuI ACTGGG 1 cut(s) 295
BpiI GAAGAC 2 cut(s) 542, 1038
BpmI CTGGAG 1 cut(s) 397
Bpu10I CCTNAGC 1 cut(s) 163
Bpu14I TTCGAA 1 cut(s) 825
BpuEI CTTGAG 2 cut(s) 1337, 1438
BpuMI CCSGG 2 cut(s) 1441, 1442
BsaHI GRCGYC 2 cut(s) 474, 882
BsaI GGTCTC 2 cut(s) 786, 1022
BsaJI CCNNGG 4 cut(s) 419, 459, 506, 1440
Bsc4I CCNNNNNNNGG 4 cut(s) 303, 420, 530, 735
Bse118I RCCGGY 2 cut(s) 251, 476
Bse1I ACTGG 1 cut(s) 301
Bse3DI GCAATG 1 cut(s) 119
BseBI CCWGG 3 cut(s) 699, 736, 897
BseDI CCNNGG 4 cut(s) 419, 459, 506, 1440
BseGI GGATG 4 cut(s) 113, 625, 910, 1105
BseLI CCNNNNNNNGG 4 cut(s) 303, 420, 530, 735
BseMI GCAATG 1 cut(s) 119
BseMII CTCAG 2 cut(s) 177, 864
BseNI ACTGG 1 cut(s) 301
BseRI GAGGAG 3 cut(s) 439, 572, 852
BseSI GKGCMC 2 cut(s) 251, 1198
BseXI GCAGC 1 cut(s) 451
BseYI CCCAGC 2 cut(s) 171, 764
Bsh1236I CGCG 2 cut(s) 337, 339
BshFI GGCC 5 cut(s) 192, 200, 255, 382, 566
BshNI GGYRCC 3 cut(s) 248, 865, 1195
BsiHKAI GWGCWC 3 cut(s) 456, 582, 1065
BsiHKCI CYCGRG 1 cut(s) 1440
BsiSI CCGG 3 cut(s) 252, 477, 1441
BslFI GGGAC 4 cut(s) 485, 711, 1305, 1423
BslI CCNNNNNNNGG 4 cut(s) 303, 420, 530, 735
BsmAI GTCTC 5 cut(s) 786, 889, 986, 1022, 1413
BsmBI CGTCTC 1 cut(s) 889
BsmFI GGGAC 4 cut(s) 485, 711, 1305, 1423
BsmI GAATGC 1 cut(s) 1333
BsnI GGCC 5 cut(s) 192, 200, 255, 382, 566
Bso31I GGTCTC 2 cut(s) 786, 1022
BsoBI CYCGRG 1 cut(s) 1440
Bsp119I TTCGAA 1 cut(s) 825
Bsp1286I GDGCHC 5 cut(s) 251, 456, 582, 1065, 1198
Bsp143I GATC 6 cut(s) 342, 511, 1070, 1234, 1320, 1480
BspACI CCGC 5 cut(s) 351, 429, 432, 435, 1297
BspANI GGCC 5 cut(s) 192, 200, 255, 382, 566
BspCNI CTCAG 2 cut(s) 176, 865
BspFNI CGCG 2 cut(s) 337, 339
BspHI TCATGA 1 cut(s) 856
BspLI GGNNCC 7 cut(s) 220, 250, 727, 867, 1197, 1363, 1482
BspMI ACCTGC 1 cut(s) 1322
BspPI GGATC 4 cut(s) 337, 519, 1475, 1488
BspQI GCTCTTC 1 cut(s) 1269
BspT104I TTCGAA 1 cut(s) 825
BspT107I GGYRCC 3 cut(s) 248, 865, 1195
BspTNI GGTCTC 2 cut(s) 786, 1022
BsrBI CCGCTC 1 cut(s) 429
BsrDI GCAATG 1 cut(s) 119
BsrFI RCCGGY 2 cut(s) 251, 476
BsrI ACTGG 1 cut(s) 301
BssAI RCCGGY 2 cut(s) 251, 476
BssECI CCNNGG 4 cut(s) 419, 459, 506, 1440
BssMI GATC 6 cut(s) 342, 511, 1070, 1234, 1320, 1480
BssNI GRCGYC 2 cut(s) 474, 882
BssSI CACGAG 2 cut(s) 518, 1125
BssT1I CCWWGG 1 cut(s) 506
Bst2BI CACGAG 2 cut(s) 518, 1125
Bst2UI CCWGG 3 cut(s) 699, 736, 897
Bst4CI ACNGT 3 cut(s) 980, 1369, 1402
Bst6I CTCTTC 4 cut(s) 88, 147, 1015, 1269
BstACI GRCGYC 2 cut(s) 474, 882
BstBI TTCGAA 1 cut(s) 825
BstC8I GCNNGC 2 cut(s) 456, 1117
BstDEI CTNAG 3 cut(s) 163, 873, 1231
BstDSI CCRYGG 1 cut(s) 419
BstEII GGTNACC 1 cut(s) 842
BstF5I GGATG 4 cut(s) 113, 625, 910, 1105
BstFNI CGCG 2 cut(s) 337, 339
BstH2I RGCGCY 1 cut(s) 705
BstHHI GCGC 2 cut(s) 339, 704
BstKTI GATC 6 cut(s) 345, 514, 1073, 1237, 1323, 1483
BstMAI GTCTC 5 cut(s) 786, 889, 986, 1022, 1413
BstMBI GATC 6 cut(s) 342, 511, 1070, 1234, 1320, 1480
BstMWI GCNNNNNNNGC 8 cut(s) 130, 181, 336, 464, 473, 761, 1100, 1536
BstNI CCWGG 3 cut(s) 699, 736, 897
BstPI GGTNACC 1 cut(s) 842
BstSCI CCNGG 5 cut(s) 697, 734, 895, 1439, 1440
BstSLI GKGCMC 2 cut(s) 251, 1198
BstUI CGCG 2 cut(s) 337, 339
BstV1I GCAGC 1 cut(s) 451
BstV2I GAAGAC 2 cut(s) 542, 1038
BstX2I RGATCY 4 cut(s) 1070, 1234, 1320, 1480
BstYI RGATCY 4 cut(s) 1070, 1234, 1320, 1480
BsuRI GGCC 5 cut(s) 192, 200, 255, 382, 566
BtgI CCRYGG 1 cut(s) 419
BtrI CACGTC 1 cut(s) 442
BtsCI GGATG 4 cut(s) 113, 625, 910, 1105
BtsIMutI CAGTG 5 cut(s) 308, 816, 1063, 1365, 1451
BveI ACCTGC 1 cut(s) 1322
Cac8I GCNNGC 2 cut(s) 456, 1117
CaiI CAGNNNCTG 1 cut(s) 704
CciI TCATGA 1 cut(s) 856
CfoI GCGC 2 cut(s) 339, 704
Cfr10I RCCGGY 2 cut(s) 251, 476
Cfr13I GGNCC 4 cut(s) 218, 381, 565, 725
Cfr9I CCCGGG 1 cut(s) 1440
CseI GACGC 1 cut(s) 482
CsiI ACCWGGT 1 cut(s) 734
Csp6I GTAC 5 cut(s) 391, 547, 866, 998, 1122
CviAII CATG 4 cut(s) 196, 202, 857, 1288
CviQI GTAC 5 cut(s) 391, 547, 866, 998, 1122
DdeI CTNAG 3 cut(s) 163, 873, 1231
DpnI GATC 6 cut(s) 344, 513, 1072, 1236, 1322, 1482
DpnII GATC 6 cut(s) 342, 511, 1070, 1234, 1320, 1480
DraIII CACNNNGTG 2 cut(s) 303, 738
EaeI YGGCCR 3 cut(s) 190, 198, 253
Eam1104I CTCTTC 4 cut(s) 88, 147, 1015, 1269
EarI CTCTTC 4 cut(s) 88, 147, 1015, 1269
Ecl136II GAGCTC 3 cut(s) 454, 580, 1063
Eco130I CCWWGG 1 cut(s) 506
Eco24I GRGCYC 3 cut(s) 456, 582, 1065
Eco31I GGTCTC 2 cut(s) 786, 1022
Eco32I GATATC 3 cut(s) 601, 1376, 1458
Eco47I GGWCC 2 cut(s) 218, 725
Eco53kI GAGCTC 3 cut(s) 454, 580, 1063
Eco88I CYCGRG 1 cut(s) 1440
Eco91I GGTNACC 1 cut(s) 842
EcoICRI GAGCTC 3 cut(s) 454, 580, 1063
EcoO65I GGTNACC 1 cut(s) 842
EcoRI GAATTC 3 cut(s) 554, 1052, 1562
EcoRII CCWGG 3 cut(s) 697, 734, 895
EcoRV GATATC 3 cut(s) 601, 1376, 1458
EcoT14I CCWWGG 1 cut(s) 506
EcoT38I GRGCYC 3 cut(s) 456, 582, 1065
ErhI CCWWGG 1 cut(s) 506
Esp3I CGTCTC 1 cut(s) 889
FaeI CATG 4 cut(s) 199, 205, 860, 1291
FaqI GGGAC 4 cut(s) 485, 711, 1305, 1423
FatI CATG 4 cut(s) 195, 201, 856, 1287
FauI CCCGC 1 cut(s) 1290
FblI GTMKAC 1 cut(s) 770
Fnu4HI GCNGC 3 cut(s) 430, 433, 465
FokI GGATG 4 cut(s) 100, 632, 897, 1112
FriOI GRGCYC 3 cut(s) 456, 582, 1065
Fsp4HI GCNGC 3 cut(s) 430, 433, 465
FspBI CTAG 3 cut(s) 468, 1091, 1247
GlaI GCGC 2 cut(s) 338, 703
GluI GCNGC 3 cut(s) 430, 433, 465
GsaI CCCAGC 2 cut(s) 175, 768
GsuI CTGGAG 1 cut(s) 397
HaeII RGCGCY 1 cut(s) 705
HaeIII GGCC 5 cut(s) 192, 200, 255, 382, 566
HapII CCGG 3 cut(s) 252, 477, 1441
HgaI GACGC 1 cut(s) 482
HhaI GCGC 2 cut(s) 339, 704
Hin1I GRCGYC 2 cut(s) 474, 882
Hin1II CATG 4 cut(s) 199, 205, 860, 1291
Hin6I GCGC 2 cut(s) 337, 702
HinP1I GCGC 2 cut(s) 337, 702
HinfI GANTC 7 cut(s) 42, 236, 323, 665, 682, 940, 1087
HpaII CCGG 3 cut(s) 252, 477, 1441
HphI GGTGA 7 cut(s) 223, 340, 449, 461, 824, 854, 1544
Hpy166II GTNNAC 3 cut(s) 393, 771, 1124
Hpy188I TCNGA 7 cut(s) 218, 241, 681, 718, 919, 1067, 1380
Hpy188III TCNNGA 8 cut(s) 39, 728, 834, 857, 1241, 1316, 1324, 1410
Hpy8I GTNNAC 3 cut(s) 393, 771, 1124
Hpy99I CGWCG 1 cut(s) 752
HpyAV CCTTC 5 cut(s) 68, 238, 629, 637, 1169
HpyCH4III ACNGT 3 cut(s) 980, 1369, 1402
HpyCH4IV ACGT 2 cut(s) 441, 882
HpyCH4V TGCA 4 cut(s) 626, 935, 1331, 1390
HpyF10VI GCNNNNNNNGC 8 cut(s) 130, 181, 336, 464, 473, 761, 1100, 1536
HpyF3I CTNAG 3 cut(s) 163, 873, 1231
HpySE526I ACGT 2 cut(s) 441, 882
Hsp92I GRCGYC 2 cut(s) 474, 882
Hsp92II CATG 4 cut(s) 199, 205, 860, 1291
HspAI GCGC 2 cut(s) 337, 702
KpnI GGTACC 1 cut(s) 869
Kzo9I GATC 6 cut(s) 342, 511, 1070, 1234, 1320, 1480
LguI GCTCTTC 1 cut(s) 1269
LmnI GCTCC 6 cut(s) 172, 180, 416, 426, 585, 1068
Lsp1109I GCAGC 1 cut(s) 451
LweI GCATC 4 cut(s) 709, 919, 1090, 1539
MabI ACCWGGT 1 cut(s) 734
MaeI CTAG 3 cut(s) 468, 1091, 1247
MaeII ACGT 2 cut(s) 441, 882
MaeIII GTNAC 6 cut(s) 437, 812, 842, 878, 987, 1351
MalI GATC 6 cut(s) 344, 513, 1072, 1236, 1322, 1482
MbiI CCGCTC 1 cut(s) 429
MboI GATC 6 cut(s) 342, 511, 1070, 1234, 1320, 1480
MflI RGATCY 4 cut(s) 1070, 1234, 1320, 1480
MhlI GDGCHC 5 cut(s) 251, 456, 582, 1065, 1198
MlsI TGGCCA 1 cut(s) 200
MluNI TGGCCA 1 cut(s) 200
MlyI GAGTC 3 cut(s) 230, 317, 934
MmeI TCCRAC 2 cut(s) 597, 1421
Mox20I TGGCCA 1 cut(s) 200
MroXI GAANNNNTTC 2 cut(s) 1035, 1179
MscI TGGCCA 1 cut(s) 200
MseI TTAA 3 cut(s) 1047, 1404, 1496
MslI CAYNNNNRTG 3 cut(s) 200, 363, 1040
Msp20I TGGCCA 1 cut(s) 200
MspA1I CMGCKG 2 cut(s) 872, 1297
MspI CCGG 3 cut(s) 252, 477, 1441
MspR9I CCNGG 5 cut(s) 699, 736, 897, 1441, 1442
Mva1269I GAATGC 1 cut(s) 1333
MvaI CCWGG 3 cut(s) 699, 736, 897
MvnI CGCG 2 cut(s) 337, 339
MwoI GCNNNNNNNGC 8 cut(s) 130, 181, 336, 464, 473, 761, 1100, 1536
NciI CCSGG 2 cut(s) 1441, 1442
NdeII GATC 6 cut(s) 342, 511, 1070, 1234, 1320, 1480
NlaIII CATG 4 cut(s) 199, 205, 860, 1291
NlaIV GGNNCC 7 cut(s) 220, 250, 727, 867, 1197, 1363, 1482
NmeAIII GCCGAG 2 cut(s) 168, 484
NmuCI GTSAC 4 cut(s) 437, 812, 842, 878
NspV TTCGAA 1 cut(s) 825
PagI TCATGA 1 cut(s) 856
PaqCI CACCTGC 1 cut(s) 1322
PciSI GCTCTTC 1 cut(s) 1269
PctI GAATGC 1 cut(s) 1333
PdmI GAANNNNTTC 2 cut(s) 1035, 1179
PfeI GAWTC 4 cut(s) 42, 665, 682, 1087
PflMI CCANNNNNTGG 2 cut(s) 303, 420
PkrI GCNGC 3 cut(s) 431, 434, 466
PleI GAGTC 3 cut(s) 230, 317, 934
PpsI GAGTC 3 cut(s) 230, 317, 934
Psp124BI GAGCTC 3 cut(s) 456, 582, 1065
Psp6I CCWGG 3 cut(s) 697, 734, 895
PspEI GGTNACC 1 cut(s) 842
PspFI CCCAGC 2 cut(s) 171, 764
PspGI CCWGG 3 cut(s) 697, 734, 895
PspN4I GGNNCC 7 cut(s) 220, 250, 727, 867, 1197, 1363, 1482
PspPI GGNCC 4 cut(s) 218, 381, 565, 725
PstNI CAGNNNCTG 1 cut(s) 704
PsuI RGATCY 4 cut(s) 1070, 1234, 1320, 1480
PvuII CAGCTG 1 cut(s) 872
RsaI GTAC 5 cut(s) 392, 548, 867, 999, 1123
RsaNI GTAC 5 cut(s) 391, 547, 866, 998, 1122
RseI CAYNNNNRTG 3 cut(s) 200, 363, 1040
SacI GAGCTC 3 cut(s) 456, 582, 1065
SapI GCTCTTC 1 cut(s) 1269
SaqAI TTAA 3 cut(s) 1047, 1404, 1496
SatI GCNGC 3 cut(s) 430, 433, 465
Sau3AI GATC 6 cut(s) 342, 511, 1070, 1234, 1320, 1480
Sau96I GGNCC 4 cut(s) 218, 381, 565, 725
SchI GAGTC 3 cut(s) 230, 317, 934
ScrFI CCNGG 5 cut(s) 699, 736, 897, 1441, 1442
SduI GDGCHC 5 cut(s) 251, 456, 582, 1065, 1198
SexAI ACCWGGT 1 cut(s) 734
SfaNI GCATC 4 cut(s) 709, 919, 1090, 1539
SfuI TTCGAA 1 cut(s) 825
SinI GGWCC 2 cut(s) 218, 725
SmaI CCCGGG 1 cut(s) 1442
SmiMI CAYNNNNRTG 3 cut(s) 200, 363, 1040
SmlI CTYRAG 2 cut(s) 1316, 1417
SmoI CTYRAG 2 cut(s) 1316, 1417
SpeI ACTAGT 1 cut(s) 1246
SsiI CCGC 5 cut(s) 351, 429, 432, 435, 1297
SspI AATATT 1 cut(s) 830
SspMI CTAG 3 cut(s) 468, 1091, 1247
SstI GAGCTC 3 cut(s) 456, 582, 1065
StyD4I CCNGG 5 cut(s) 697, 734, 895, 1439, 1440
StyI CCWWGG 1 cut(s) 506
TaaI ACNGT 3 cut(s) 980, 1369, 1402
TaiI ACGT 2 cut(s) 444, 885
TaqI TCGA 2 cut(s) 825, 1411
TaqII GACCGA 1 cut(s) 394
TatI WGTACW 3 cut(s) 390, 546, 1121
TauI GCSGC 2 cut(s) 432, 435
TfiI GAWTC 4 cut(s) 42, 665, 682, 1087
Tru1I TTAA 3 cut(s) 1047, 1404, 1496
Tru9I TTAA 3 cut(s) 1047, 1404, 1496
TscAI CASTG 5 cut(s) 308, 823, 1063, 1372, 1458
TseFI GTSAC 4 cut(s) 437, 812, 842, 878
TseI GCWGC 1 cut(s) 464
Tsp45I GTSAC 4 cut(s) 437, 812, 842, 878
TspDTI ATGAA 5 cut(s) 17, 102, 567, 674, 1172
TspMI CCCGGG 1 cut(s) 1440
TspRI CASTG 5 cut(s) 308, 823, 1063, 1372, 1458
Van91I CCANNNNNTGG 2 cut(s) 303, 420
VpaK11BI GGWCC 2 cut(s) 218, 725
XapI RAATTY 3 cut(s) 554, 1052, 1562
XmaI CCCGGG 1 cut(s) 1440
XmiI GTMKAC 1 cut(s) 770
XmnI GAANNNNTTC 2 cut(s) 1035, 1179
XspI CTAG 3 cut(s) 468, 1091, 1247
ZraI GACGTC 1 cut(s) 883
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.