Basic Information

Type: gene

Biological Identity

Species

rosa_chinensis

Chromosome

Orthogroup ID

OG0013651

Physical Location & Seq

Genomic Coordinates Forward (+)

4695808 .. 4699571

Gene Structure 3764 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

N/A

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1290 bp

ATGATGACGACTAGCAGCAACCAAGTTGTGAAAGTTAAGAGGGAGAAGCTCGAGGCATGCATGACATGCCCCATGTGCAAAGGGCTATTCGAAGAAGCCACAACTATTTCTGAATGTCTTCACACATTTTGCAAGAAGTGCATATATGATAAAATAATCAATGAAGATTTGGATAGATGTCCAGTATGCAAGACAGATTTAGGGGTTGCTCCAATGGATAAGCTCAGGGCTGACAATAGTCTCGAAGACATAAGGGCGAAGCTCTTCCCTTCTGGAAGTAAAAAGGTCAAGGCACAAGCAGCGGACCGCAATCTGCAAGCTGTAGGCTCTGAAAAGGTCTTGCCTTCAGAAACAAGAAAAACCAAGGCACCTGAAGTTAATGTGCCCTCCATATTGTTACCAGGGAGAAGAAAGCAGAGATACCTCTCTTCATTAGGAACAAGCGCTCCTACAGTTACAACCAGGTCCTCTATGAAGTATTTTACCAGAAAGAGTTCAGCTCTTCAAGAGAGTCATCTTTCGACTGAAGAAGAGCCTGTTCACAAACATGAAGATTTTCCGGAGAGGGTTAAATCATCTGAGAACCTTAAAGAAGATGCAGCACATGCCAGACAGAATGCTACTGAGAAATTGTCTCAGAAAGATGGGGAGAATAATGGCAACGAGAACAAAGCACAACCAAGGTGGACAACTGGGCTGATGGAAGCTGAAAGAGAAGCTGCAAGAGTGCCAATAAGGCAAGTGTTCCTCAACCTCAACAATGAACCAGAAGAACTTCAACCCATTCAGGAACATGGCAATAATGGTGAATCTACTCCTGCACCATCAGTTTCAGTGCCAAGTGCCAAAGGCCAACGTGGCCGCAAAAGAAAAGCAGTATCAGAACCCGAGGAGTTAAAAACTCCAGCACAATCTGTGGTATCTGACGCAAACAATGAAGGTGAAACAATATTTAAACCAATCTGGTTCTCGTTGGAAGCTTATCCATGGGAAGGATATGCACCCTTGCCACAGATACCTTCATGTTACTTGAGAGTCAAAGATGGTAACTTACCCAGTTCTTTCATAAAGAAGTATCTGGTGAAAAAGCTGAATCTTCACAGCGAGGCGGAGGTGGAGATTCTATTGTGGGGTCGGCCGATACATCCCACATTGCAGCTGCATAACTTGGTGGATTTATGGTTACAGAAAATACCAGCATCACATAAGATAGAATCCTTTGTAGGCAGCTCGGCAAAGGAGTACGTGATGGTTATAACCTACTGTCGAAAAGTGCTACAGCACTGGTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0001101 GO:0003674 GO:0003824 GO:0004842 GO:0006464 GO:0006807 GO:0006950 GO:0008150 GO:0008152 GO:0009414 GO:0009415 GO:0009628 GO:0009987 GO:0010035 GO:0016567 GO:0016740 GO:0019538 GO:0019787 GO:0032446 GO:0036211 GO:0042221 GO:0043170 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0050896 GO:0051865 GO:0070647 GO:0071704 GO:0140096 GO:1901564 GO:1901700

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

zf-C3HC4_2

Protein Analysis

429

Amino Acids

48.26

Weight (kDa)

8.74

Isoelectric Point (pI)

55.4

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
zf-C3HC4_2	PF13923	23 - 63	2.4e-08	Zinc finger, C3HC4 type (RING finger)
zf-C3HC4	PF00097	23 - 63	9.9e-07	Zinc finger, C3HC4 type (RING finger)
zf-RING_UBOX	PF13445	23 - 60	6.4e-06	RING-type zinc-finger

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

Font Size (px)

Line Width

Align Labels (Cladogram)

Image

Tree File

Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready

iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0013651)

Species	Orthologous Gene IDs
fragaria_vesca	FvH4_4g02340 FvH4_4g02340
malus_domestica	MD13G1205400.v1.1 MD16G1207100.v1.1
prunus_persica	Prupe.1G028000_v2.0.a1 Prupe.1G028000_v2.0.a1 Prupe.1G028000_v2.0.a1 Prupe.1G028000_v2.0.a1
rosa_chinensis	RchiOBHm_Chr4g0389721
rosa_laevigata	RLG00000009960
rosa_multiflora	Rmu_sc0003144.1_g000010
rosa_roxburghii	Rroxscaffold_5G00335720
rosa_rugosa	Rorug03G0324900
rosa_samantha	Rh4AG029800 Rh4BG022900 Rh4CG031600 Rh4DG023200
rosa_wichuraiana	Rw4G002230

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	1256
AccB1I	GGYRCC	1 cut(s)	367
AccIII	TCCGGA	1 cut(s)	559
AciI	CCGC	4 cut(s)	302, 307, 862, 1109
AcoI	YGGCCR	2 cut(s)	859, 1136
AcuI	CTGAAG	3 cut(s)	330, 393, 546
AfaI	GTAC	1 cut(s)	1244
AfeI	AGCGCT	1 cut(s)	445
AfiI	CCNNNNNNNGG	1 cut(s)	992
AgsI	TTSAA	2 cut(s)	506, 779
AjnI	CCWGG	2 cut(s)	400, 461
AloI	GAACNNNNNNTCC	2 cut(s)	430, 462
Alw26I	GTCTC	2 cut(s)	245, 639
Ama87I	CYCGRG	2 cut(s)	50, 887
Aor13HI	TCCGGA	1 cut(s)	559
Aor51HI	AGCGCT	1 cut(s)	445
AoxI	GGCC	3 cut(s)	850, 859, 1136
ApeKI	GCWGC	7 cut(s)	15, 299, 599, 719, 1156, 1159, 1227
Asp700I	GAANNNNTTC	5 cut(s)	117, 263, 493, 555, 774
AspLEI	GCGC	1 cut(s)	446
AspS9I	GGNCC	2 cut(s)	304, 465
AsuHPI	GGTGA	3 cut(s)	818, 953, 1093
AsuII	TTCGAA	1 cut(s)	90
AvaI	CYCGRG	2 cut(s)	50, 887
AvaII	GGWCC	2 cut(s)	304, 465
BaeGI	GKGCMC	1 cut(s)	387
BanI	GGYRCC	1 cut(s)	367
BbsI	GAAGAC	2 cut(s)	110, 252
BbvI	GCAGC	7 cut(s)	27, 311, 611, 706, 1146, 1168, 1239
BccI	CCATC	5 cut(s)	638, 694, 832, 1037, 1243
BciT130I	CCWGG	2 cut(s)	402, 463
BcoDI	GTCTC	2 cut(s)	245, 639
BfaI	CTAG	1 cut(s)	12
BfmI	CTRYAG	3 cut(s)	321, 450, 1277
BfoI	RGCGCY	1 cut(s)	447
BglI	GCCNNNNNGGC	2 cut(s)	736, 858
BisI	GCNGC	8 cut(s)	16, 300, 600, 720, 862, 1157, 1160, 1228
BlsI	GCNGC	8 cut(s)	17, 301, 601, 721, 863, 1158, 1161, 1229
Bme1390I	CCNGG	2 cut(s)	402, 463
Bme18I	GGWCC	2 cut(s)	304, 465
BmeT110I	CYCGRG	2 cut(s)	50, 887
BmgT120I	GGNCC	2 cut(s)	304, 465
BmiI	GGNNCC	1 cut(s)	369
BmrFI	CCNGG	2 cut(s)	402, 463
BmrI	ACTGGG	2 cut(s)	702, 1050
BmsI	GCATC	2 cut(s)	586, 1208
BmuI	ACTGGG	2 cut(s)	702, 1050
BoxI	GACNNNNGTC	1 cut(s)	237
BpiI	GAAGAC	2 cut(s)	110, 252
BplI	GAGNNNNNCTC	2 cut(s)	484, 516
BpmI	CTGGAG	1 cut(s)	888
Bpu10I	CCTNAGC	1 cut(s)	224
Bpu14I	TTCGAA	1 cut(s)	90
BpuEI	CTTGAG	1 cut(s)	1051
BsaAI	YACGTR	1 cut(s)	1246
BsaJI	CCNNGG	5 cut(s)	363, 401, 680, 888, 986
BsaWI	WCCGGW	1 cut(s)	559
BsaXI	ACNNNNNCTCC	2 cut(s)	430, 460
Bsc4I	CCNNNNNNNGG	1 cut(s)	992
Bse1I	ACTGG	4 cut(s)	182, 697, 1056, 1289
Bse3DI	GCAATG	1 cut(s)	1151
BseAI	TCCGGA	1 cut(s)	559
BseBI	CCWGG	2 cut(s)	402, 463
BseDI	CCNNGG	5 cut(s)	363, 401, 680, 888, 986
BseGI	GGATG	1 cut(s)	1144
BseLI	CCNNNNNNNGG	1 cut(s)	992
BseMI	GCAATG	1 cut(s)	1151
BseMII	CTCAG	4 cut(s)	238, 570, 615, 650
BseNI	ACTGG	4 cut(s)	182, 697, 1056, 1289
BseRI	GAGGAG	1 cut(s)	905
BseSI	GKGCMC	1 cut(s)	387
BseX3I	CGGCCG	1 cut(s)	1136
BseXI	GCAGC	7 cut(s)	27, 311, 611, 706, 1146, 1168, 1239
BsgI	GTGCAG	1 cut(s)	804
Bsh1285I	CGRYCG	1 cut(s)	1139
BshFI	GGCC	3 cut(s)	852, 861, 1138
BshNI	GGYRCC	1 cut(s)	367
BsiEI	CGRYCG	1 cut(s)	1139
BsiHKCI	CYCGRG	2 cut(s)	50, 887
BsiSI	CCGG	1 cut(s)	560
BslI	CCNNNNNNNGG	1 cut(s)	992
BsmAI	GTCTC	2 cut(s)	245, 639
BsmI	GAATGC	1 cut(s)	622
BsnI	GGCC	3 cut(s)	852, 861, 1138
BsoBI	CYCGRG	2 cut(s)	50, 887
Bsp119I	TTCGAA	1 cut(s)	90
Bsp1286I	GDGCHC	1 cut(s)	387
Bsp13I	TCCGGA	1 cut(s)	559
Bsp19I	CCATGG	1 cut(s)	986
BspACI	CCGC	4 cut(s)	302, 307, 862, 1109
BspANI	GGCC	3 cut(s)	852, 861, 1138
BspCNI	CTCAG	4 cut(s)	237, 571, 616, 649
BspEI	TCCGGA	1 cut(s)	559
BspLI	GGNNCC	1 cut(s)	369
BspQI	GCTCTTC	3 cut(s)	269, 507, 525
BspT104I	TTCGAA	1 cut(s)	90
BspT107I	GGYRCC	1 cut(s)	367
BsrDI	GCAATG	1 cut(s)	1151
BsrI	ACTGG	4 cut(s)	182, 697, 1056, 1289
BssECI	CCNNGG	5 cut(s)	363, 401, 680, 888, 986
BssT1I	CCWWGG	3 cut(s)	363, 680, 986
Bst2UI	CCWGG	2 cut(s)	402, 463
Bst4CI	ACNGT	2 cut(s)	454, 1265
Bst6I	CTCTTC	4 cut(s)	269, 433, 507, 525
BstAPI	GCANNNNNTGC	3 cut(s)	66, 138, 605
BstBAI	YACGTR	1 cut(s)	1246
BstBI	TTCGAA	1 cut(s)	90
BstC8I	GCNNGC	2 cut(s)	58, 318
BstDEI	CTNAG	4 cut(s)	224, 579, 624, 636
BstDSI	CCRYGG	1 cut(s)	986
BstF5I	GGATG	1 cut(s)	1144
BstH2I	RGCGCY	1 cut(s)	447
BstHHI	GCGC	1 cut(s)	446
BstMAI	GTCTC	2 cut(s)	245, 639
BstMCI	CGRYCG	1 cut(s)	1139
BstMWI	GCNNNNNNNGC	7 cut(s)	66, 75, 138, 299, 605, 736, 858
BstNI	CCWGG	2 cut(s)	402, 463
BstNSI	RCATGY	3 cut(s)	60, 69, 608
BstPAI	GACNNNNGTC	1 cut(s)	237
BstSCI	CCNGG	2 cut(s)	400, 461
BstSFI	CTRYAG	3 cut(s)	321, 450, 1277
BstSLI	GKGCMC	1 cut(s)	387
BstV1I	GCAGC	7 cut(s)	27, 311, 611, 706, 1146, 1168, 1239
BstV2I	GAAGAC	2 cut(s)	110, 252
BstZI	CGGCCG	1 cut(s)	1136
BsuRI	GGCC	3 cut(s)	852, 861, 1138
BtgI	CCRYGG	1 cut(s)	986
BtsCI	GGATG	1 cut(s)	1144
BtsIMutI	CAGTG	2 cut(s)	840, 1282
Cac8I	GCNNGC	2 cut(s)	58, 318
CfoI	GCGC	1 cut(s)	446
Cfr13I	GGNCC	2 cut(s)	304, 465
CpoI	CGGWCCG	1 cut(s)	304
CseI	GACGC	1 cut(s)	935
CsiI	ACCWGGT	1 cut(s)	461
Csp6I	GTAC	1 cut(s)	1243
CspCI	CAANNNNNGTGG	2 cut(s)	665, 700
CspI	CGGWCCG	1 cut(s)	304
CviAII	CATG	9 cut(s)	57, 61, 66, 73, 548, 605, 794, 987, 1023
CviQI	GTAC	1 cut(s)	1243
DdeI	CTNAG	4 cut(s)	224, 579, 624, 636
DraI	TTTAAA	1 cut(s)	955
EaeI	YGGCCR	2 cut(s)	859, 1136
EagI	CGGCCG	1 cut(s)	1136
Eam1104I	CTCTTC	4 cut(s)	269, 433, 507, 525
EarI	CTCTTC	4 cut(s)	269, 433, 507, 525
EciI	GGCGGA	1 cut(s)	1124
EclXI	CGGCCG	1 cut(s)	1136
Eco130I	CCWWGG	3 cut(s)	363, 680, 986
Eco47I	GGWCC	2 cut(s)	304, 465
Eco47III	AGCGCT	1 cut(s)	445
Eco52I	CGGCCG	1 cut(s)	1136
Eco57I	CTGAAG	3 cut(s)	330, 393, 546
Eco88I	CYCGRG	2 cut(s)	50, 887
EcoO109I	RGGNCCY	1 cut(s)	465
EcoRII	CCWGG	2 cut(s)	400, 461
EcoT14I	CCWWGG	3 cut(s)	363, 680, 986
EcoT22I	ATGCAT	1 cut(s)	62
ErhI	CCWWGG	3 cut(s)	363, 680, 986
FaeI	CATG	9 cut(s)	60, 64, 69, 76, 551, 608, 797, 990, 1026
FatI	CATG	9 cut(s)	56, 60, 65, 72, 547, 604, 793, 986, 1022
Fnu4HI	GCNGC	8 cut(s)	16, 300, 600, 720, 862, 1157, 1160, 1228
FokI	GGATG	1 cut(s)	1131
Fsp4HI	GCNGC	8 cut(s)	16, 300, 600, 720, 862, 1157, 1160, 1228
FspBI	CTAG	1 cut(s)	12
GlaI	GCGC	1 cut(s)	445
GluI	GCNGC	8 cut(s)	16, 300, 600, 720, 862, 1157, 1160, 1228
GsuI	CTGGAG	1 cut(s)	888
HaeII	RGCGCY	1 cut(s)	447
HaeIII	GGCC	3 cut(s)	852, 861, 1138
HapII	CCGG	1 cut(s)	560
HgaI	GACGC	1 cut(s)	935
HhaI	GCGC	1 cut(s)	446
Hin1II	CATG	9 cut(s)	60, 64, 69, 76, 551, 608, 797, 990, 1026
Hin6I	GCGC	1 cut(s)	444
HinP1I	GCGC	1 cut(s)	444
HindIII	AAGCTT	1 cut(s)	978
HinfI	GANTC	6 cut(s)	511, 809, 1035, 1093, 1120, 1214
HpaII	CCGG	1 cut(s)	560
HphI	GGTGA	3 cut(s)	818, 953, 1093
Hpy166II	GTNNAC	2 cut(s)	541, 687
Hpy188I	TCNGA	7 cut(s)	112, 331, 349, 580, 639, 883, 925
Hpy188III	TCNNGA	5 cut(s)	242, 273, 506, 560, 788
Hpy8I	GTNNAC	2 cut(s)	541, 687
HpyAV	CCTTC	5 cut(s)	279, 354, 932, 986, 1029
HpyCH4III	ACNGT	2 cut(s)	454, 1265
HpyCH4IV	ACGT	2 cut(s)	856, 1245
HpyF10VI	GCNNNNNNNGC	7 cut(s)	66, 75, 138, 299, 605, 736, 858
HpyF3I	CTNAG	4 cut(s)	224, 579, 624, 636
HpySE526I	ACGT	2 cut(s)	856, 1245
Hsp92II	CATG	9 cut(s)	60, 64, 69, 76, 551, 608, 797, 990, 1026
HspAI	GCGC	1 cut(s)	444
Kpn2I	TCCGGA	1 cut(s)	559
LguI	GCTCTTC	3 cut(s)	269, 507, 525
LmnI	GCTCC	2 cut(s)	214, 451
Lsp1109I	GCAGC	7 cut(s)	27, 311, 611, 706, 1146, 1168, 1239
LweI	GCATC	2 cut(s)	586, 1208
MabI	ACCWGGT	1 cut(s)	461
MaeI	CTAG	1 cut(s)	12
MaeII	ACGT	2 cut(s)	856, 1245
MaeIII	GTNAC	5 cut(s)	396, 454, 1025, 1046, 1182
MhlI	GDGCHC	1 cut(s)	387
MluCI	AATT	1 cut(s)	629
MlyI	GAGTC	2 cut(s)	520, 1044
MmeI	TCCRAC	1 cut(s)	954
Mph1103I	ATGCAT	1 cut(s)	62
MroI	TCCGGA	1 cut(s)	559
MroXI	GAANNNNTTC	5 cut(s)	117, 263, 493, 555, 774
MseI	TTAA	6 cut(s)	36, 378, 570, 588, 896, 954
MslI	CAYNNNNRTG	1 cut(s)	546
MspA1I	CMGCKG	2 cut(s)	302, 1159
MspI	CCGG	1 cut(s)	560
MspR9I	CCNGG	2 cut(s)	402, 463
Mva1269I	GAATGC	1 cut(s)	622
MvaI	CCWGG	2 cut(s)	402, 463
MwoI	GCNNNNNNNGC	7 cut(s)	66, 75, 138, 299, 605, 736, 858
NcoI	CCATGG	1 cut(s)	986
NlaIII	CATG	9 cut(s)	60, 64, 69, 76, 551, 608, 797, 990, 1026
NlaIV	GGNNCC	1 cut(s)	369
NmeAIII	GCCGAG	1 cut(s)	1211
NsiI	ATGCAT	1 cut(s)	62
NspI	RCATGY	3 cut(s)	60, 69, 608
NspV	TTCGAA	1 cut(s)	90
PaeI	GCATGC	1 cut(s)	60
PaeR7I	CTCGAG	1 cut(s)	50
PciSI	GCTCTTC	3 cut(s)	269, 507, 525
PctI	GAATGC	1 cut(s)	622
PdmI	GAANNNNTTC	5 cut(s)	117, 263, 493, 555, 774
PfeI	GAWTC	4 cut(s)	809, 1093, 1120, 1214
PkrI	GCNGC	8 cut(s)	17, 301, 601, 721, 863, 1158, 1161, 1229
PleI	GAGTC	2 cut(s)	519, 1043
PpsI	GAGTC	2 cut(s)	519, 1043
Ppu21I	YACGTR	1 cut(s)	1246
PpuMI	RGGWCCY	1 cut(s)	465
PshAI	GACNNNNGTC	1 cut(s)	237
PsiI	TTATAA	1 cut(s)	1256
Psp5II	RGGWCCY	1 cut(s)	465
Psp6I	CCWGG	2 cut(s)	400, 461
PspGI	CCWGG	2 cut(s)	400, 461
PspN4I	GGNNCC	1 cut(s)	369
PspPI	GGNCC	2 cut(s)	304, 465
PspPPI	RGGWCCY	1 cut(s)	465
PspXI	VCTCGAGB	1 cut(s)	50
PvuII	CAGCTG	1 cut(s)	1159
RsaI	GTAC	1 cut(s)	1244
RsaNI	GTAC	1 cut(s)	1243
RseI	CAYNNNNRTG	1 cut(s)	546
Rsr2I	CGGWCCG	1 cut(s)	304
RsrII	CGGWCCG	1 cut(s)	304
SapI	GCTCTTC	3 cut(s)	269, 507, 525
SaqAI	TTAA	6 cut(s)	36, 378, 570, 588, 896, 954
SatI	GCNGC	8 cut(s)	16, 300, 600, 720, 862, 1157, 1160, 1228
Sau96I	GGNCC	2 cut(s)	304, 465
SchI	GAGTC	2 cut(s)	520, 1044
ScrFI	CCNGG	2 cut(s)	402, 463
SduI	GDGCHC	1 cut(s)	387
SexAI	ACCWGGT	1 cut(s)	461
SfaNI	GCATC	2 cut(s)	586, 1208
SfcI	CTRYAG	3 cut(s)	321, 450, 1277
SfiI	GGCCNNNNNGGCC	1 cut(s)	858
Sfr274I	CTCGAG	1 cut(s)	50
SfuI	TTCGAA	1 cut(s)	90
SinI	GGWCC	2 cut(s)	304, 465
SlaI	CTCGAG	1 cut(s)	50
SmiMI	CAYNNNNRTG	1 cut(s)	546
SmlI	CTYRAG	2 cut(s)	50, 1030
SmoI	CTYRAG	2 cut(s)	50, 1030
SphI	GCATGC	1 cut(s)	60
Sse9I	AATT	1 cut(s)	629
SsiI	CCGC	4 cut(s)	302, 307, 862, 1109
SspI	AATATT	1 cut(s)	951
SspMI	CTAG	1 cut(s)	12
StyD4I	CCNGG	2 cut(s)	400, 461
StyI	CCWWGG	3 cut(s)	363, 680, 986
TaaI	ACNGT	2 cut(s)	454, 1265
TaiI	ACGT	2 cut(s)	859, 1248
TaqI	TCGA	5 cut(s)	51, 90, 243, 521, 1267
TasI	AATT	1 cut(s)	629
TauI	GCSGC	1 cut(s)	864
TfiI	GAWTC	4 cut(s)	809, 1093, 1120, 1214
Tru1I	TTAA	6 cut(s)	36, 378, 570, 588, 896, 954
Tru9I	TTAA	6 cut(s)	36, 378, 570, 588, 896, 954
TscAI	CASTG	2 cut(s)	840, 1289
TseI	GCWGC	7 cut(s)	15, 299, 599, 719, 1156, 1159, 1227
TspDTI	ATGAA	8 cut(s)	177, 420, 488, 564, 777, 951, 1011, 1054
TspRI	CASTG	2 cut(s)	840, 1289
VpaK11BI	GGWCC	2 cut(s)	304, 465
XceI	RCATGY	3 cut(s)	60, 69, 608
XhoI	CTCGAG	1 cut(s)	50
XmnI	GAANNNNTTC	5 cut(s)	117, 263, 493, 555, 774
XspI	CTAG	1 cut(s)	12
Zsp2I	ATGCAT	1 cut(s)	62

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.