Basic Information

Type: gene

Biological Identity

Species

rosa_samantha

Chromosome

Chr4D

Orthogroup ID

OG0013651

Physical Location & Seq

Genomic Coordinates Forward (+)

3760759 .. 3784051

Gene Structure 23293 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rh4DG023200.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2208 bp

ATGGTAACTGTTCGTGGAGATGAGAATGACGTTGACGAACTTGAAATGTTACTGGAGGCTTACTTTATGCAGATTGATGGCGCCTTGAACAAGTTAGCTACGCTGCGTGAATATATTGATGATACGGAGGATTACATAAATATTCAGCTCGATAACCACAGGAATCAGTTAATTCAGCTTGAGCTTATTCTTAGTTCTGGAACTGTTAGTTTATCCATGTATTCTTTGGTGTGTGGAATTTTTGGCGTGAATATCCCATATTCGTGGAACGAGGATCATGGATACATGTTTAAATGGGTTTGCATTGTTACAGGAATAGTATGTTTCTCTGTTTTCATAGTAATTGTAGCATATGCTCGCCACAAAGGGTCGTTTCATGGTTTTTCAGCCCTCCAAGGCCGCTTTGTGTCAGTAAACTCCCAAACAGATCAGCCTCTCTATCAAACTGAATTACTCGACCTAGAACTCTGCTTTTCTCATGGAGAATTTCGGCGGAAAACCGGCCTTCTGTGTTGGGGTGTGGCATTCCGGTGCTTGATCGCTGGGAAGGATAGTTCGGGTCAGGTCCGACCACAACGAGGGCGGCTCAAGAATGATTGGTCAAAATTGCAGATGATAAGCAGATCTGAAGAGCTGCGAGGGATGATGGGGCAAGTGGTTCAAGGTTCCTACGCCCAGCGTACAGCGTCGGGGGGTTTTGGGCTTCGAATTGCAGAAGCGGTGGTGGAATACCATGGCGGGGAGGTTGGATCTGCGACGAAGTGGCTGAGGAAAGTGACGATGGGCTTCGTGACTGTGACAACAGCGGCGGCGGAAAGCTACCAGATCTTGGGGGCTAGGGCTGCGCGCCTAAGGGTTGGAAAACGGGAACGGCTGCTTCCCAGTGCTCTGTTTATCAGTGGGTTTTGGAAGAAGATAATGATGACGACTAGCAGCAACCAAGTTGTGAAAGTTAAGAGGGAGAAGCTCGAGGCATGCATGACATGCCCCATGTGCAAAGAGCTATTCGAAGAAGCCACAACTATTTCTGAATGTCTTCACACATTTTGCAAGAAGTGCATATATGATAAAATAATCAACGAAGATTTGGATAGATGTCCAGTATGCAAGACAGATTTAGGGGTTGCTCCAATGGATAAGCTCAGGGCTGACAATAGTCTCGAAGACATAAGGGCGAAGCTCTTCCCTTCTGGAAGTAAAAAGGTCAAGGCACAAGCAGCGGACCGCAATCTGCAAGTTGTAGGCTCTGAAAAGGTCTTGCCTTCAGAAAAAAGAAAAACCAAGGCACCTGAAATTAATGTGCCCTCCATATTGTTACCAGGGAGAAGAAAGCAGAGATACCTCTCTTCATTAGGAACAAGCGCTCCTACAGTTACAACCAGGTCCTCTACGAGGTATTTTACCAGAAAGAGTTCAGCTCTTCAAGAGAGTCATCTTTCGACTCAAGAAGAGCCTGTTCACAAACATGAAGATTTTCCGGAGAGGGTTAAATCATCTGAGAACCTTAAAGAAGATGCAGCACAAGCCAGACAGAATGCTACTGAGAAATTGTCTCAGAAAGATGGGGAGAATAATGGCAACGAGAACAAAGCACAACCAAGGTGGACAACTGGGCTGATGGAAGCTGAAAGAGAAGCTGCAAGAGTGCCAATAAGGCAAGTGTTCCTCAACCTCAACAATGAACCAGAAGAGCTTCAACCCATTCAGGAACATGGCAATAATGGTGAATCTACTCCTGCACCATCAGTTTCAGTGCCAAGTGCCAAAGGCCAACGTGGCCGCAAGAGAAAAGCAGTATCAGAACCCGAGGAGTTAAAAACTCCAGCACAACCTGTGGTATCTGACGCAAACAATGAAGGCGAAACAACATTTAAACCAATCTGGTTCTCGTTGGAAGCTTATCCATGGGAAGGATATGCACCCTTGCCACAGATACCTTCATGTTACTTGAGAGTCAAAGATGGTAACTTACCCAGTTCTTTCATAAAGAAGTATCTGGTGAAAAAGCTGAATCTTCACAGCGAGGCGGAGGTGGAGATTCTATTGTGGGGTCGGCCGATACATCCCACATTGCAGCTGCATAACTTGGTGGATTTATGGCTACAGAAAATACCAGCATCACATAAGATAGAATCCTTTGTAGGCAGCTCGGCAAAGGAGTACGTGATGGTTATAACCTACTGTCGAAAAGTGCTACAGCACTGGTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0001101 GO:0003674 GO:0003824 GO:0004842 GO:0006464 GO:0006807 GO:0006950 GO:0008150 GO:0008152 GO:0009414 GO:0009415 GO:0009628 GO:0009987 GO:0010035 GO:0016567 GO:0016740 GO:0019538 GO:0019787 GO:0032446 GO:0036211 GO:0042221 GO:0043170 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0050896 GO:0051865 GO:0070647 GO:0071704 GO:0140096 GO:1901564 GO:1901700

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

zf-C3HC4_2

Protein Analysis

735

Amino Acids

82.73

Weight (kDa)

8.72

Isoelectric Point (pI)

45.62

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
MRS2-like	PF22099	7 - 54	3.1e-07	Magnesium transporter MRS2-like
zf-C3HC4_2	PF13923	329 - 369	3.6e-08	Zinc finger, C3HC4 type (RING finger)
zf-C3HC4	PF00097	329 - 369	1.2e-06	Zinc finger, C3HC4 type (RING finger)
zf-RING_UBOX	PF13445	329 - 366	1.2e-06	RING-type zinc-finger

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0013651)

Species	Orthologous Gene IDs
fragaria_vesca	FvH4_4g02340 FvH4_4g02340
malus_domestica	MD13G1205400.v1.1 MD16G1207100.v1.1
prunus_persica	Prupe.1G028000_v2.0.a1 Prupe.1G028000_v2.0.a1 Prupe.1G028000_v2.0.a1 Prupe.1G028000_v2.0.a1
rosa_chinensis	RchiOBHm_Chr4g0389721
rosa_laevigata	RLG00000009960
rosa_multiflora	Rmu_sc0003144.1_g000010
rosa_roxburghii	Rroxscaffold_5G00335720
rosa_rugosa	Rorug03G0324900
rosa_samantha	Rh4AG029800 Rh4BG022900 Rh4CG031600 Rh4DG023200
rosa_wichuraiana	Rw4G002230

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	2174
AccB1I	GGYRCC	2 cut(s)	80, 1285
AccB7I	CCANNNNNTGG	1 cut(s)	829
AccII	CGCG	1 cut(s)	847
AccIII	TCCGGA	1 cut(s)	1477
AclWI	GGATC	2 cut(s)	282, 757
AcoI	YGGCCR	2 cut(s)	1777, 2054
AcsI	RAATTY	2 cut(s)	237, 485
AcuI	CTGAAG	2 cut(s)	648, 1248
AcyI	GRCGYC	1 cut(s)	81
AfaI	GTAC	2 cut(s)	682, 2162
AfeI	AGCGCT	1 cut(s)	1363
AfiI	CCNNNNNNNGG	5 cut(s)	578, 739, 829, 1392, 1910
AflIII	ACRYGT	1 cut(s)	285
AgsI	TTSAA	5 cut(s)	44, 88, 662, 1424, 1697
AjnI	CCWGG	2 cut(s)	1318, 1379
AloI	GAACNNNNNNTCC	4 cut(s)	538, 570, 1348, 1380
Alw21I	GWGCWC	1 cut(s)	889
Alw26I	GTCTC	2 cut(s)	1163, 1557
AlwI	GGATC	2 cut(s)	282, 757
Ama87I	CYCGRG	2 cut(s)	968, 1805
Aor13HI	TCCGGA	1 cut(s)	1477
Aor51HI	AGCGCT	1 cut(s)	1363
AoxI	GGCC	5 cut(s)	397, 502, 1768, 1777, 2054
ApoI	RAATTY	2 cut(s)	237, 485
AseI	ATTAAT	1 cut(s)	1296
Asp700I	GAANNNNTTC	5 cut(s)	1035, 1181, 1411, 1473, 1692
AspLEI	GCGC	4 cut(s)	83, 847, 849, 1364
AspS9I	GGNCC	3 cut(s)	565, 1222, 1383
AsuHPI	GGTGA	2 cut(s)	1736, 2011
AsuII	TTCGAA	2 cut(s)	706, 1008
AvaI	CYCGRG	2 cut(s)	968, 1805
AvaII	GGWCC	3 cut(s)	565, 1222, 1383
AxyI	CCTNAGG	1 cut(s)	851
BaeGI	GKGCMC	1 cut(s)	1305
BanI	GGYRCC	2 cut(s)	80, 1285
BbsI	GAAGAC	2 cut(s)	1028, 1170
Bbv12I	GWGCWC	1 cut(s)	889
BbvCI	CCTCAGC	1 cut(s)	767
BccI	CCATC	8 cut(s)	71, 640, 775, 1556, 1612, 1750, 1955, 2161
BceAI	ACGGC	1 cut(s)	887
BciT130I	CCWGG	2 cut(s)	1320, 1381
BciVI	GTATCC	1 cut(s)	275
BcoDI	GTCTC	2 cut(s)	1163, 1557
BfaI	CTAG	3 cut(s)	461, 837, 930
BfmI	CTRYAG	3 cut(s)	1368, 2102, 2195
BfoI	RGCGCY	2 cut(s)	84, 1365
BfuI	GTATCC	1 cut(s)	275
BglI	GCCNNNNNGGC	2 cut(s)	1654, 1776
BglII	AGATCT	2 cut(s)	623, 825
Bme1390I	CCNGG	2 cut(s)	1320, 1381
Bme18I	GGWCC	3 cut(s)	565, 1222, 1383
BmeT110I	CYCGRG	2 cut(s)	968, 1805
BmgT120I	GGNCC	3 cut(s)	565, 1222, 1383
BmiI	GGNNCC	3 cut(s)	82, 667, 1287
BmrFI	CCNGG	2 cut(s)	1320, 1381
BmrI	ACTGGG	3 cut(s)	876, 1620, 1968
BmsI	GCATC	2 cut(s)	1504, 2126
BmuI	ACTGGG	3 cut(s)	876, 1620, 1968
BoxI	GACNNNNGTC	1 cut(s)	1155
BpiI	GAAGAC	2 cut(s)	1028, 1170
BplI	GAGNNNNNCTC	4 cut(s)	570, 602, 1402, 1434
BpmI	CTGGAG	2 cut(s)	74, 1806
Bpu10I	CCTNAGC	2 cut(s)	767, 1142
Bpu14I	TTCGAA	2 cut(s)	706, 1008
BpuEI	CTTGAG	4 cut(s)	200, 572, 1428, 1969
BsaAI	YACGTR	1 cut(s)	2164
BsaHI	GRCGYC	1 cut(s)	81
BsaJI	CCNNGG	7 cut(s)	394, 733, 1281, 1319, 1598, 1806, 1904
BsaWI	WCCGGW	2 cut(s)	528, 1477
BsaXI	ACNNNNNCTCC	2 cut(s)	1348, 1378
Bsc4I	CCNNNNNNNGG	5 cut(s)	578, 739, 829, 1392, 1910
Bse118I	RCCGGY	1 cut(s)	500
Bse1I	ACTGG	6 cut(s)	57, 882, 1100, 1615, 1974, 2207
Bse21I	CCTNAGG	1 cut(s)	851
Bse3DI	GCAATG	1 cut(s)	2069
BseAI	TCCGGA	1 cut(s)	1477
BseBI	CCWGG	2 cut(s)	1320, 1381
BseDI	CCNNGG	7 cut(s)	394, 733, 1281, 1319, 1598, 1806, 1904
BseGI	GGATG	2 cut(s)	648, 2062
BseLI	CCNNNNNNNGG	5 cut(s)	578, 739, 829, 1392, 1910
BseMI	GCAATG	1 cut(s)	2069
BseMII	CTCAG	5 cut(s)	758, 1156, 1488, 1533, 1568
BseNI	ACTGG	6 cut(s)	57, 882, 1100, 1615, 1974, 2207
BsePI	GCGCGC	1 cut(s)	845
BseRI	GAGGAG	1 cut(s)	1823
BseSI	GKGCMC	1 cut(s)	1305
BseX3I	CGGCCG	1 cut(s)	2054
BseYI	CCCAGC	2 cut(s)	542, 675
BsgI	GTGCAG	1 cut(s)	1722
Bsh1236I	CGCG	1 cut(s)	847
Bsh1285I	CGRYCG	1 cut(s)	2057
BshFI	GGCC	5 cut(s)	399, 504, 1770, 1779, 2056
BshNI	GGYRCC	2 cut(s)	80, 1285
BsiEI	CGRYCG	1 cut(s)	2057
BsiHKAI	GWGCWC	1 cut(s)	889
BsiHKCI	CYCGRG	2 cut(s)	968, 1805
BsiSI	CCGG	3 cut(s)	501, 529, 1478
BslI	CCNNNNNNNGG	5 cut(s)	578, 739, 829, 1392, 1910
BsmAI	GTCTC	2 cut(s)	1163, 1557
BsmI	GAATGC	2 cut(s)	524, 1540
BsnI	GGCC	5 cut(s)	399, 504, 1770, 1779, 2056
BsoBI	CYCGRG	2 cut(s)	968, 1805
Bsp119I	TTCGAA	2 cut(s)	706, 1008
Bsp1286I	GDGCHC	2 cut(s)	889, 1305
Bsp13I	TCCGGA	1 cut(s)	1477
Bsp143I	GATC	6 cut(s)	274, 427, 537, 623, 749, 825
Bsp19I	CCATGG	2 cut(s)	733, 1904
BspANI	GGCC	5 cut(s)	399, 504, 1770, 1779, 2056
BspCNI	CTCAG	5 cut(s)	759, 1155, 1489, 1534, 1567
BspEI	TCCGGA	1 cut(s)	1477
BspFNI	CGCG	1 cut(s)	847
BspLI	GGNNCC	3 cut(s)	82, 667, 1287
BspPI	GGATC	2 cut(s)	282, 757
BspQI	GCTCTTC	5 cut(s)	624, 1187, 1425, 1443, 1683
BspT104I	TTCGAA	2 cut(s)	706, 1008
BspT107I	GGYRCC	2 cut(s)	80, 1285
BsrDI	GCAATG	1 cut(s)	2069
BsrFI	RCCGGY	1 cut(s)	500
BsrI	ACTGG	6 cut(s)	57, 882, 1100, 1615, 1974, 2207
BssAI	RCCGGY	1 cut(s)	500
BssECI	CCNNGG	7 cut(s)	394, 733, 1281, 1319, 1598, 1806, 1904
BssHII	GCGCGC	1 cut(s)	845
BssMI	GATC	6 cut(s)	274, 427, 537, 623, 749, 825
BssNI	GRCGYC	1 cut(s)	81
BssT1I	CCWWGG	5 cut(s)	394, 733, 1281, 1598, 1904
Bst2UI	CCWGG	2 cut(s)	1320, 1381
Bst4CI	ACNGT	5 cut(s)	10, 205, 796, 1372, 2183
Bst6I	CTCTTC	6 cut(s)	624, 1187, 1351, 1425, 1443, 1683
BstACI	GRCGYC	1 cut(s)	81
BstAPI	GCANNNNNTGC	2 cut(s)	984, 1056
BstBAI	YACGTR	1 cut(s)	2164
BstBI	TTCGAA	2 cut(s)	706, 1008
BstC8I	GCNNGC	3 cut(s)	358, 847, 976
BstDEI	CTNAG	7 cut(s)	191, 767, 851, 1142, 1497, 1542, 1554
BstDSI	CCRYGG	2 cut(s)	733, 1904
BstENI	CCTNNNNNAGG	1 cut(s)	1390
BstF5I	GGATG	2 cut(s)	648, 2062
BstFNI	CGCG	1 cut(s)	847
BstH2I	RGCGCY	2 cut(s)	84, 1365
BstHHI	GCGC	4 cut(s)	83, 847, 849, 1364
BstKTI	GATC	6 cut(s)	277, 430, 540, 626, 752, 828
BstMAI	GTCTC	2 cut(s)	1163, 1557
BstMBI	GATC	6 cut(s)	274, 427, 537, 623, 749, 825
BstMCI	CGRYCG	1 cut(s)	2057
BstMWI	GCNNNNNNNGC	8 cut(s)	842, 984, 993, 1056, 1217, 1523, 1654, 1776
BstNI	CCWGG	2 cut(s)	1320, 1381
BstNSI	RCATGY	3 cut(s)	289, 978, 987
BstPAI	GACNNNNGTC	1 cut(s)	1155
BstSCI	CCNGG	2 cut(s)	1318, 1379
BstSFI	CTRYAG	3 cut(s)	1368, 2102, 2195
BstSLI	GKGCMC	1 cut(s)	1305
BstUI	CGCG	1 cut(s)	847
BstV2I	GAAGAC	2 cut(s)	1028, 1170
BstX2I	RGATCY	3 cut(s)	623, 749, 825
BstXI	CCANNNNNNTGG	1 cut(s)	264
BstYI	RGATCY	3 cut(s)	623, 749, 825
BstZI	CGGCCG	1 cut(s)	2054
Bsu36I	CCTNAGG	1 cut(s)	851
BsuI	GTATCC	1 cut(s)	275
BsuRI	GGCC	5 cut(s)	399, 504, 1770, 1779, 2056
BtgI	CCRYGG	2 cut(s)	733, 1904
BtsCI	GGATG	2 cut(s)	648, 2062
BtsIMutI	CAGTG	4 cut(s)	889, 904, 1758, 2200
Cac8I	GCNNGC	3 cut(s)	358, 847, 976
CfoI	GCGC	4 cut(s)	83, 847, 849, 1364
Cfr10I	RCCGGY	1 cut(s)	500
Cfr13I	GGNCC	3 cut(s)	565, 1222, 1383
CpoI	CGGWCCG	1 cut(s)	1222
CseI	GACGC	2 cut(s)	675, 1853
CsiI	ACCWGGT	1 cut(s)	1379
Csp6I	GTAC	2 cut(s)	681, 2161
CspCI	CAANNNNNGTGG	2 cut(s)	1583, 1618
CspI	CGGWCCG	1 cut(s)	1222
CviQI	GTAC	2 cut(s)	681, 2161
DdeI	CTNAG	7 cut(s)	191, 767, 851, 1142, 1497, 1542, 1554
DinI	GGCGCC	1 cut(s)	82
DpnI	GATC	6 cut(s)	276, 429, 539, 625, 751, 827
DpnII	GATC	6 cut(s)	274, 427, 537, 623, 749, 825
DraI	TTTAAA	2 cut(s)	292, 1873
EaeI	YGGCCR	2 cut(s)	1777, 2054
EagI	CGGCCG	1 cut(s)	2054
Eam1104I	CTCTTC	6 cut(s)	624, 1187, 1351, 1425, 1443, 1683
EarI	CTCTTC	6 cut(s)	624, 1187, 1351, 1425, 1443, 1683
EciI	GGCGGA	3 cut(s)	508, 827, 2042
EclXI	CGGCCG	1 cut(s)	2054
Eco130I	CCWWGG	5 cut(s)	394, 733, 1281, 1598, 1904
Eco47I	GGWCC	3 cut(s)	565, 1222, 1383
Eco47III	AGCGCT	1 cut(s)	1363
Eco52I	CGGCCG	1 cut(s)	2054
Eco57I	CTGAAG	2 cut(s)	648, 1248
Eco81I	CCTNAGG	1 cut(s)	851
Eco88I	CYCGRG	2 cut(s)	968, 1805
EcoNI	CCTNNNNNAGG	1 cut(s)	1390
EcoO109I	RGGNCCY	1 cut(s)	1383
EcoRII	CCWGG	2 cut(s)	1318, 1379
EcoT14I	CCWWGG	5 cut(s)	394, 733, 1281, 1598, 1904
EcoT22I	ATGCAT	1 cut(s)	980
EgeI	GGCGCC	1 cut(s)	82
EheI	GGCGCC	1 cut(s)	82
ErhI	CCWWGG	5 cut(s)	394, 733, 1281, 1598, 1904
FauI	CCCGC	1 cut(s)	731
FauNDI	CATATG	1 cut(s)	352
FokI	GGATG	2 cut(s)	655, 2049
FspBI	CTAG	3 cut(s)	461, 837, 930
GlaI	GCGC	4 cut(s)	82, 846, 848, 1363
GsaI	CCCAGC	2 cut(s)	546, 679
GsuI	CTGGAG	2 cut(s)	74, 1806
HaeII	RGCGCY	2 cut(s)	84, 1365
HaeIII	GGCC	5 cut(s)	399, 504, 1770, 1779, 2056
HapII	CCGG	3 cut(s)	501, 529, 1478
HgaI	GACGC	2 cut(s)	675, 1853
HhaI	GCGC	4 cut(s)	83, 847, 849, 1364
Hin1I	GRCGYC	1 cut(s)	81
Hin6I	GCGC	4 cut(s)	81, 845, 847, 1362
HinP1I	GCGC	4 cut(s)	81, 845, 847, 1362
HincII	GTYRAC	1 cut(s)	34
HindII	GTYRAC	1 cut(s)	34
HindIII	AAGCTT	1 cut(s)	1896
HinfI	GANTC	8 cut(s)	163, 1429, 1441, 1727, 1953, 2011, 2038, 2132
HpaII	CCGG	3 cut(s)	501, 529, 1478
HphI	GGTGA	2 cut(s)	1736, 2011
Hpy166II	GTNNAC	4 cut(s)	34, 415, 1459, 1605
Hpy188I	TCNGA	9 cut(s)	569, 628, 1030, 1249, 1267, 1498, 1557, 1801, 1843
Hpy188III	TCNNGA	9 cut(s)	198, 589, 790, 1160, 1191, 1424, 1445, 1478, 1706
Hpy8I	GTNNAC	4 cut(s)	34, 415, 1459, 1605
Hpy99I	CGWCG	2 cut(s)	691, 760
HpyAV	CCTTC	7 cut(s)	515, 541, 1197, 1272, 1850, 1904, 1947
HpyCH4III	ACNGT	5 cut(s)	10, 205, 796, 1372, 2183
HpyCH4IV	ACGT	3 cut(s)	30, 1774, 2163
HpyF10VI	GCNNNNNNNGC	8 cut(s)	842, 984, 993, 1056, 1217, 1523, 1654, 1776
HpyF3I	CTNAG	7 cut(s)	191, 767, 851, 1142, 1497, 1542, 1554
HpySE526I	ACGT	3 cut(s)	30, 1774, 2163
Hsp92I	GRCGYC	1 cut(s)	81
HspAI	GCGC	4 cut(s)	81, 845, 847, 1362
KasI	GGCGCC	1 cut(s)	80
Kpn2I	TCCGGA	1 cut(s)	1477
Kzo9I	GATC	6 cut(s)	274, 427, 537, 623, 749, 825
LguI	GCTCTTC	5 cut(s)	624, 1187, 1425, 1443, 1683
LmnI	GCTCC	2 cut(s)	1132, 1369
LweI	GCATC	2 cut(s)	1504, 2126
MabI	ACCWGGT	1 cut(s)	1379
MaeI	CTAG	3 cut(s)	461, 837, 930
MaeII	ACGT	3 cut(s)	30, 1774, 2163
MalI	GATC	6 cut(s)	276, 429, 539, 625, 751, 827
MboI	GATC	6 cut(s)	274, 427, 537, 623, 749, 825
MflI	RGATCY	3 cut(s)	623, 749, 825
MhlI	GDGCHC	2 cut(s)	889, 1305
MluCI	AATT	9 cut(s)	171, 237, 342, 449, 485, 605, 708, 1293, 1547
Mly113I	GGCGCC	1 cut(s)	81
MlyI	GAGTC	3 cut(s)	1435, 1438, 1962
MmeI	TCCRAC	4 cut(s)	592, 727, 838, 1872
Mph1103I	ATGCAT	1 cut(s)	980
MroI	TCCGGA	1 cut(s)	1477
MroXI	GAANNNNTTC	5 cut(s)	1035, 1181, 1411, 1473, 1692
MseI	TTAA	8 cut(s)	170, 291, 954, 1296, 1488, 1506, 1814, 1872
MslI	CAYNNNNRTG	3 cut(s)	262, 529, 1464
MspA1I	CMGCKG	3 cut(s)	806, 1220, 2077
MspI	CCGG	3 cut(s)	501, 529, 1478
MspR9I	CCNGG	2 cut(s)	1320, 1381
Mva1269I	GAATGC	2 cut(s)	524, 1540
MvaI	CCWGG	2 cut(s)	1320, 1381
MvnI	CGCG	1 cut(s)	847
MwoI	GCNNNNNNNGC	8 cut(s)	842, 984, 993, 1056, 1217, 1523, 1654, 1776
NarI	GGCGCC	1 cut(s)	81
NcoI	CCATGG	2 cut(s)	733, 1904
NdeI	CATATG	1 cut(s)	352
NdeII	GATC	6 cut(s)	274, 427, 537, 623, 749, 825
NlaIV	GGNNCC	3 cut(s)	82, 667, 1287
NmeAIII	GCCGAG	1 cut(s)	2129
NmuCI	GTSAC	3 cut(s)	775, 790, 796
NsiI	ATGCAT	1 cut(s)	980
NspI	RCATGY	3 cut(s)	289, 978, 987
NspV	TTCGAA	2 cut(s)	706, 1008
PaeI	GCATGC	1 cut(s)	978
PaeR7I	CTCGAG	1 cut(s)	968
PauI	GCGCGC	1 cut(s)	845
PciI	ACATGT	1 cut(s)	285
PciSI	GCTCTTC	5 cut(s)	624, 1187, 1425, 1443, 1683
PctI	GAATGC	2 cut(s)	524, 1540
PdmI	GAANNNNTTC	5 cut(s)	1035, 1181, 1411, 1473, 1692
PfeI	GAWTC	5 cut(s)	163, 1727, 2011, 2038, 2132
PflMI	CCANNNNNTGG	1 cut(s)	829
PleI	GAGTC	3 cut(s)	1435, 1437, 1961
PluTI	GGCGCC	1 cut(s)	84
PpsI	GAGTC	3 cut(s)	1435, 1437, 1961
Ppu21I	YACGTR	1 cut(s)	2164
PpuMI	RGGWCCY	1 cut(s)	1383
PscI	ACATGT	1 cut(s)	285
PshAI	GACNNNNGTC	1 cut(s)	1155
PshBI	ATTAAT	1 cut(s)	1296
PsiI	TTATAA	1 cut(s)	2174
Psp5II	RGGWCCY	1 cut(s)	1383
Psp6I	CCWGG	2 cut(s)	1318, 1379
PspFI	CCCAGC	2 cut(s)	542, 675
PspGI	CCWGG	2 cut(s)	1318, 1379
PspN4I	GGNNCC	3 cut(s)	82, 667, 1287
PspPI	GGNCC	3 cut(s)	565, 1222, 1383
PspPPI	RGGWCCY	1 cut(s)	1383
PspXI	VCTCGAGB	1 cut(s)	968
PsuI	RGATCY	3 cut(s)	623, 749, 825
PteI	GCGCGC	1 cut(s)	845
PvuII	CAGCTG	1 cut(s)	2077
RsaI	GTAC	2 cut(s)	682, 2162
RsaNI	GTAC	2 cut(s)	681, 2161
RseI	CAYNNNNRTG	3 cut(s)	262, 529, 1464
Rsr2I	CGGWCCG	1 cut(s)	1222
RsrII	CGGWCCG	1 cut(s)	1222
SapI	GCTCTTC	5 cut(s)	624, 1187, 1425, 1443, 1683
SaqAI	TTAA	8 cut(s)	170, 291, 954, 1296, 1488, 1506, 1814, 1872
Sau3AI	GATC	6 cut(s)	274, 427, 537, 623, 749, 825
Sau96I	GGNCC	3 cut(s)	565, 1222, 1383
SchI	GAGTC	3 cut(s)	1435, 1438, 1962
ScrFI	CCNGG	2 cut(s)	1320, 1381
SduI	GDGCHC	2 cut(s)	889, 1305
SexAI	ACCWGGT	1 cut(s)	1379
SfaNI	GCATC	2 cut(s)	1504, 2126
SfcI	CTRYAG	3 cut(s)	1368, 2102, 2195
SfiI	GGCCNNNNNGGCC	1 cut(s)	1776
SfoI	GGCGCC	1 cut(s)	82
Sfr274I	CTCGAG	1 cut(s)	968
SfuI	TTCGAA	2 cut(s)	706, 1008
SinI	GGWCC	3 cut(s)	565, 1222, 1383
SlaI	CTCGAG	1 cut(s)	968
SmiMI	CAYNNNNRTG	3 cut(s)	262, 529, 1464
SmlI	CTYRAG	5 cut(s)	179, 587, 968, 1443, 1948
SmoI	CTYRAG	5 cut(s)	179, 587, 968, 1443, 1948
SphI	GCATGC	1 cut(s)	978
Sse9I	AATT	9 cut(s)	171, 237, 342, 449, 485, 605, 708, 1293, 1547
SspDI	GGCGCC	1 cut(s)	80
SspI	AATATT	1 cut(s)	142
SspMI	CTAG	3 cut(s)	461, 837, 930
StyD4I	CCNGG	2 cut(s)	1318, 1379
StyI	CCWWGG	5 cut(s)	394, 733, 1281, 1598, 1904
TaaI	ACNGT	5 cut(s)	10, 205, 796, 1372, 2183
TaiI	ACGT	3 cut(s)	33, 1777, 2166
TaqI	TCGA	8 cut(s)	150, 456, 706, 969, 1008, 1161, 1439, 2185
TasI	AATT	9 cut(s)	171, 237, 342, 449, 485, 605, 708, 1293, 1547
TauI	GCSGC	5 cut(s)	402, 586, 809, 812, 1782
TfiI	GAWTC	5 cut(s)	163, 1727, 2011, 2038, 2132
Tru1I	TTAA	8 cut(s)	170, 291, 954, 1296, 1488, 1506, 1814, 1872
Tru9I	TTAA	8 cut(s)	170, 291, 954, 1296, 1488, 1506, 1814, 1872
TscAI	CASTG	4 cut(s)	889, 904, 1758, 2207
TseFI	GTSAC	3 cut(s)	775, 790, 796
Tsp45I	GTSAC	3 cut(s)	775, 790, 796
TspDTI	ATGAA	8 cut(s)	325, 365, 1338, 1482, 1695, 1869, 1929, 1972
TspGWI	ACGGA	1 cut(s)	140
TspRI	CASTG	4 cut(s)	889, 904, 1758, 2207
Van91I	CCANNNNNTGG	1 cut(s)	829
VpaK11BI	GGWCC	3 cut(s)	565, 1222, 1383
VspI	ATTAAT	1 cut(s)	1296
XagI	CCTNNNNNAGG	1 cut(s)	1390
XapI	RAATTY	2 cut(s)	237, 485
XceI	RCATGY	3 cut(s)	289, 978, 987
XcmI	CCANNNNNNNNNTGG	1 cut(s)	223
XhoI	CTCGAG	1 cut(s)	968
XmnI	GAANNNNTTC	5 cut(s)	1035, 1181, 1411, 1473, 1692
XspI	CTAG	3 cut(s)	461, 837, 930
Zsp2I	ATGCAT	1 cut(s)	980

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.