RchiOBHm_Chr4g0442141

ascorbate-specific transmembrane electron transporter

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
4
OG0017659
Physical Location & Seq
Forward (+)
63839623 .. 63840638
1016 bp
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UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 627 bp
ATGGCGCCCAAGAGTCGTAGCTTCCAAGTTTCAGCAACCCCAGTTACCATAATTGCGCATTTGTTACTTATAGCTATCACAACCCTTCTCCTTGTTTGGCTCGTACACTTGCGCGAAGGCTTTGCTTTCAACTCTTCCACGAAAGCAAAAATTTTCAATGTACTAATTAGTACTGCAGCTATCATGGCATACAAGACAATCCCAGGAAAAAGGGATACACAAAAAGTGGTGCACATGATTTTGCATCTATTAGCTCTGGTATCTGTATCTTTGGGGCTTTTTGTAATCATCAAATACAAAAATGAAGTGGGAGAGACAGATTTTATTTCATTACACTCCTGGTTGGGCATAATCACAATCTCTTTATTTGGTCTGCAGTGGCTGTTTGGTTTCGTTGCTTACATCTTTCCTGGTTCAGAGATGTCAACAAGGCCATCATTTAAGCCATGGCACACTTTCTTAGGGATGGTCATTTTTCTTCTAGCAGTGTGCACTGCTGAGATAGGGCTTAATTATTTCGTCTTAGGGATCAACCAAGAAGGACTTATAGTCAACTTCACTGGCCTTCTGATCTTCTTGTTTGCAGCTGCAGTCTCCCTCACTGTCCTCCTCCCTCGACTATACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0008150 GO:0008152 GO:0016491 GO:0055114
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Cytochrom_B561

Protein Analysis

208

Amino Acids

23.08

Weight (kDa)

9.63

Isoelectric Point (pI)

25.78

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Cytochrom_B561 PF03188 54 - 171 8.3e-36 Eukaryotic cytochrome b561
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0017659)

Species Orthologous Gene IDs
fragaria_vesca FvH4_4g33090
malus_domestica MD13G1016700.v1.1 MD16G1014600.v1.1
prunus_persica Prupe.1G336700_v2.0.a1
rosa_chinensis RchiOBHm_Chr4g0442141
rosa_laevigata RLG00000006037
rosa_roxburghii Rroxscaffold_5G00383050
rosa_rugosa Rorug04G0339200
rosa_samantha Rh4AG390800 Rh4BG404300 Rh4CG419300 Rh4DG397300

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 57
AccB1I GGYRCC 1 cut(s) 4
AccII CGCG 1 cut(s) 114
AclWI GGATC 1 cut(s) 536
AcsI RAATTY 1 cut(s) 150
AcyI GRCGYC 1 cut(s) 5
AfaI GTAC 3 cut(s) 105, 162, 172
AgsI TTSAA 2 cut(s) 130, 157
AhdI GACNNNNNGTC 1 cut(s) 548
AjnI CCWGG 3 cut(s) 202, 338, 409
AluBI AGCT 5 cut(s) 21, 74, 179, 254, 587
AluI AGCT 5 cut(s) 21, 74, 179, 254, 587
Alw21I GWGCWC 2 cut(s) 234, 494
Alw26I GTCTC 2 cut(s) 308, 598
Alw44I GTGCAC 2 cut(s) 230, 490
AlwI GGATC 1 cut(s) 536
AlwNI CAGNNNCTG 1 cut(s) 382
AoxI GGCC 2 cut(s) 431, 562
ApaLI GTGCAC 2 cut(s) 230, 490
ApeKI GCWGC 3 cut(s) 176, 584, 587
ApoI RAATTY 1 cut(s) 150
AspLEI GCGC 3 cut(s) 7, 58, 114
BaeGI GKGCMC 2 cut(s) 234, 494
BanI GGYRCC 1 cut(s) 4
Bbv12I GWGCWC 2 cut(s) 234, 494
BbvI GCAGC 3 cut(s) 188, 574, 596
BccI CCATC 2 cut(s) 442, 460
BcgI CGANNNNNNTGC 2 cut(s) 104, 138
BciT130I CCWGG 3 cut(s) 204, 340, 411
BciVI GTATCC 1 cut(s) 208
BcoDI GTCTC 2 cut(s) 308, 598
BfaI CTAG 1 cut(s) 482
BfmI CTRYAG 3 cut(s) 174, 374, 588
BfoI RGCGCY 1 cut(s) 8
BfuI GTATCC 1 cut(s) 208
BisI GCNGC 3 cut(s) 177, 585, 588
BlsI GCNGC 3 cut(s) 178, 586, 589
BmcAI AGTACT 1 cut(s) 172
Bme1390I CCNGG 3 cut(s) 204, 340, 411
BmeRI GACNNNNNGTC 1 cut(s) 548
BmiI GGNNCC 1 cut(s) 6
BmrFI CCNGG 3 cut(s) 204, 340, 411
BmrI ACTGGG 1 cut(s) 35
BmsI GCATC 1 cut(s) 253
BmuI ACTGGG 1 cut(s) 35
BsaHI GRCGYC 1 cut(s) 5
BsaJI CCNNGG 2 cut(s) 202, 446
Bse1I ACTGG 2 cut(s) 41, 565
BseBI CCWGG 3 cut(s) 204, 340, 411
BseDI CCNNGG 2 cut(s) 202, 446
BseGI GGATG 1 cut(s) 471
BseMII CTCAG 1 cut(s) 489
BseNI ACTGG 2 cut(s) 41, 565
BseRI GAGGAG 1 cut(s) 599
BseSI GKGCMC 2 cut(s) 234, 494
BseXI GCAGC 3 cut(s) 188, 574, 596
Bsh1236I CGCG 1 cut(s) 114
BshFI GGCC 2 cut(s) 433, 564
BshNI GGYRCC 1 cut(s) 4
BsiHKAI GWGCWC 2 cut(s) 234, 494
BsmAI GTCTC 2 cut(s) 308, 598
BsnI GGCC 2 cut(s) 433, 564
Bsp1286I GDGCHC 2 cut(s) 234, 494
Bsp143I GATC 2 cut(s) 528, 570
Bsp19I CCATGG 1 cut(s) 446
BspANI GGCC 2 cut(s) 433, 564
BspCNI CTCAG 1 cut(s) 490
BspFNI CGCG 1 cut(s) 114
BspLI GGNNCC 1 cut(s) 6
BspMAI CTGCAG 3 cut(s) 178, 378, 592
BspPI GGATC 1 cut(s) 536
BspT107I GGYRCC 1 cut(s) 4
BsrI ACTGG 2 cut(s) 41, 565
BssECI CCNNGG 2 cut(s) 202, 446
BssMI GATC 2 cut(s) 528, 570
BssNI GRCGYC 1 cut(s) 5
BssT1I CCWWGG 1 cut(s) 446
Bst2UI CCWGG 3 cut(s) 204, 340, 411
Bst4CI ACNGT 1 cut(s) 604
Bst6I CTCTTC 1 cut(s) 139
BstACI GRCGYC 1 cut(s) 5
BstDEI CTNAG 3 cut(s) 460, 498, 523
BstDSI CCRYGG 1 cut(s) 446
BstF5I GGATG 1 cut(s) 471
BstFNI CGCG 1 cut(s) 114
BstH2I RGCGCY 1 cut(s) 8
BstHHI GCGC 3 cut(s) 7, 58, 114
BstKTI GATC 2 cut(s) 531, 573
BstMAI GTCTC 2 cut(s) 308, 598
BstMBI GATC 2 cut(s) 528, 570
BstMWI GCNNNNNNNGC 1 cut(s) 185
BstNI CCWGG 3 cut(s) 204, 340, 411
BstSCI CCNGG 3 cut(s) 202, 338, 409
BstSFI CTRYAG 3 cut(s) 174, 374, 588
BstSLI GKGCMC 2 cut(s) 234, 494
BstUI CGCG 1 cut(s) 114
BstV1I GCAGC 3 cut(s) 188, 574, 596
BsuI GTATCC 1 cut(s) 208
BsuRI GGCC 2 cut(s) 433, 564
BtgI CCRYGG 1 cut(s) 446
BtsCI GGATG 1 cut(s) 471
BtsI GCAGTG 3 cut(s) 383, 492, 492
BtsIMutI CAGTG 5 cut(s) 383, 492, 492, 558, 600
CaiI CAGNNNCTG 1 cut(s) 382
CfoI GCGC 3 cut(s) 7, 58, 114
Csp6I GTAC 3 cut(s) 104, 161, 171
CviAII CATG 3 cut(s) 184, 235, 447
CviQI GTAC 3 cut(s) 104, 161, 171
DdeI CTNAG 3 cut(s) 460, 498, 523
DinI GGCGCC 1 cut(s) 6
DpnI GATC 2 cut(s) 530, 572
DpnII GATC 2 cut(s) 528, 570
DriI GACNNNNNGTC 1 cut(s) 548
Eam1104I CTCTTC 1 cut(s) 139
Eam1105I GACNNNNNGTC 1 cut(s) 548
EarI CTCTTC 1 cut(s) 139
Eco130I CCWWGG 1 cut(s) 446
EcoRII CCWGG 3 cut(s) 202, 338, 409
EcoT14I CCWWGG 1 cut(s) 446
EgeI GGCGCC 1 cut(s) 6
EheI GGCGCC 1 cut(s) 6
ErhI CCWWGG 1 cut(s) 446
FaeI CATG 3 cut(s) 187, 238, 450
FaiI YATR 9 cut(s) 50, 71, 185, 190, 236, 350, 448, 548, 622
FalI AAGNNNNNCTT 2 cut(s) 528, 560
FatI CATG 3 cut(s) 183, 234, 446
Fnu4HI GCNGC 3 cut(s) 177, 585, 588
FokI GGATG 1 cut(s) 478
Fsp4HI GCNGC 3 cut(s) 177, 585, 588
FspBI CTAG 1 cut(s) 482
FspI TGCGCA 1 cut(s) 57
GlaI GCGC 3 cut(s) 6, 57, 113
GluI GCNGC 3 cut(s) 177, 585, 588
HaeII RGCGCY 1 cut(s) 8
HaeIII GGCC 2 cut(s) 433, 564
HhaI GCGC 3 cut(s) 7, 58, 114
Hin1I GRCGYC 1 cut(s) 5
Hin1II CATG 3 cut(s) 187, 238, 450
Hin6I GCGC 3 cut(s) 5, 56, 112
HinP1I GCGC 3 cut(s) 5, 56, 112
HincII GTYRAC 2 cut(s) 426, 553
HindII GTYRAC 2 cut(s) 426, 553
HinfI GANTC 1 cut(s) 13
Hpy166II GTNNAC 5 cut(s) 106, 232, 426, 492, 553
Hpy188I TCNGA 2 cut(s) 418, 570
Hpy8I GTNNAC 5 cut(s) 106, 232, 426, 492, 553
HpyAV CCTTC 4 cut(s) 95, 110, 533, 575
HpyCH4III ACNGT 1 cut(s) 604
HpyCH4V TGCA 7 cut(s) 176, 232, 244, 376, 492, 584, 590
HpyF10VI GCNNNNNNNGC 1 cut(s) 185
HpyF3I CTNAG 3 cut(s) 460, 498, 523
Hsp92I GRCGYC 1 cut(s) 5
Hsp92II CATG 3 cut(s) 187, 238, 450
HspAI GCGC 3 cut(s) 5, 56, 112
KasI GGCGCC 1 cut(s) 4
Kzo9I GATC 2 cut(s) 528, 570
LpnPI CCDG 9 cut(s) 54, 189, 216, 242, 325, 352, 396, 423, 546
Lsp1109I GCAGC 3 cut(s) 188, 574, 596
LweI GCATC 1 cut(s) 253
MaeI CTAG 1 cut(s) 482
MaeIII GTNAC 2 cut(s) 43, 63
MalI GATC 2 cut(s) 530, 572
MboI GATC 2 cut(s) 528, 570
MboII GAAGA 3 cut(s) 126, 470, 565
MhlI GDGCHC 2 cut(s) 234, 494
MluCI AATT 4 cut(s) 51, 150, 165, 511
Mly113I GGCGCC 1 cut(s) 5
MlyI GAGTC 1 cut(s) 22
MnlI CCTC 4 cut(s) 608, 617, 620, 624
MseI TTAA 2 cut(s) 441, 510
MspA1I CMGCKG 1 cut(s) 587
MspR9I CCNGG 3 cut(s) 204, 340, 411
MvaI CCWGG 3 cut(s) 204, 340, 411
MvnI CGCG 1 cut(s) 114
MwoI GCNNNNNNNGC 1 cut(s) 185
NarI GGCGCC 1 cut(s) 5
NcoI CCATGG 1 cut(s) 446
NdeII GATC 2 cut(s) 528, 570
NlaIII CATG 3 cut(s) 187, 238, 450
NlaIV GGNNCC 1 cut(s) 6
NsbI TGCGCA 1 cut(s) 57
PkrI GCNGC 3 cut(s) 178, 586, 589
PleI GAGTC 1 cut(s) 21
PluTI GGCGCC 1 cut(s) 8
PpsI GAGTC 1 cut(s) 21
Psp6I CCWGG 3 cut(s) 202, 338, 409
PspGI CCWGG 3 cut(s) 202, 338, 409
PspN4I GGNNCC 1 cut(s) 6
PstI CTGCAG 3 cut(s) 178, 378, 592
PstNI CAGNNNCTG 1 cut(s) 382
PvuII CAGCTG 1 cut(s) 587
RsaI GTAC 3 cut(s) 105, 162, 172
RsaNI GTAC 3 cut(s) 104, 161, 171
SaqAI TTAA 2 cut(s) 441, 510
SatI GCNGC 3 cut(s) 177, 585, 588
Sau3AI GATC 2 cut(s) 528, 570
ScaI AGTACT 1 cut(s) 172
SchI GAGTC 1 cut(s) 22
ScrFI CCNGG 3 cut(s) 204, 340, 411
SduI GDGCHC 2 cut(s) 234, 494
SetI ASST 5 cut(s) 23, 76, 181, 256, 589
SfaNI GCATC 1 cut(s) 253
SfcI CTRYAG 3 cut(s) 174, 374, 588
SfoI GGCGCC 1 cut(s) 6
Sse9I AATT 4 cut(s) 51, 150, 165, 511
SspDI GGCGCC 1 cut(s) 4
SspMI CTAG 1 cut(s) 482
StyD4I CCNGG 3 cut(s) 202, 338, 409
StyI CCWWGG 1 cut(s) 446
TaaI ACNGT 1 cut(s) 604
TaqI TCGA 1 cut(s) 616
TasI AATT 4 cut(s) 51, 150, 165, 511
TatI WGTACW 2 cut(s) 160, 170
Tru1I TTAA 2 cut(s) 441, 510
Tru9I TTAA 2 cut(s) 441, 510
TscAI CASTG 5 cut(s) 383, 492, 499, 565, 607
TseI GCWGC 3 cut(s) 176, 584, 587
TspDTI ATGAA 2 cut(s) 318, 318
TspRI CASTG 5 cut(s) 383, 492, 499, 565, 607
VneI GTGCAC 2 cut(s) 230, 490
XapI RAATTY 1 cut(s) 150
XspI CTAG 1 cut(s) 482
ZrmI AGTACT 1 cut(s) 172
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.