RchiOBHm_Chr3g0460891

E3 ubiquitin-protein ligase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
3
OG0018171
Physical Location & Seq
Reverse (-)
8974357 .. 8975802
1446 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1446 bp
ATGGACGATATGGACCATCAGAACGATGACAGTTTCAGAGCCGGAGAGCGTATAGATGAAGAAGCATATTATGATTGCCTTGAAGTCGATGACAAATCCGATAAGTTGGGTGAGATTTGGTTCCCACAGAAAAAGAATTATACTATTCTGAAGCAAGAAGAAGTTGCCAAACTTCAGGAGGATGATATTGCAAAAGCGTCCACTTTTCTTTCGGTATCAAAATCTGATGCATGCTTACTGCTCCTTCACTCGAATTGGAGGACCGATAATCTCACCGATGCATGGTTTGCTGATGAAAATAAAATCCGCGAAAAAGCTGGCTTGTTGAAGAAACCCCTACTGGATAATGCCGGTGAAGATCTAATAAGCAGTAGTTGCCGACTATGTTCATATAGTAATAGTAGTAATATCTCAGCTTCTTGCGGTCATCCTTTTTGTGGAGATTGCTGGACAGGTTACATAACAAAATTAATCAATGAGGAGGGTCCTGCATGTTTGGTTTTGAGATGTCCTGAACCCGATTGTAGTGCAGTGGTTGGACCTGATTTAGTCCAAAACGTAATGTTGGCCACAAACCAAGAAGATGAATGTTATAAGAGGTACAAGCATTACCTATTGAGATCTTATTTAGAAGATCGCAAGTTGATCAAGTGGTGTCCTGCTCCGGATTGTAACTCCGCTATTAGGTTGGATGCTGGTGGTTCTAGTACTAGTTGCTATCATGTCTCGTGTGTTTGCTCTCGTAGTTATTGCTGGAACTGTGAGGAGGAGGAGAATCATCGTCCCGTGGACTGCCAAACTGCAGGGAAGTGGATTTCGAATATATTAGAATCCGTGCATTACCAGCGCTGGCAAAACCACGGAAAATCAAAGGAAAAAGCTCTTGAAGTATTTGACGAGGTGAAGACGACCGTGCATCAAGAATTATTAGAAGGGCACGAGTTGGATTTTTTGTTAGAGGCATGCAAACAGATGATTGAATGCAGGCAGGTATTGCGATGGATTGTTGTGTACGATTACTACAACATGAGTGATCATGAGGCGCAGCATCCTAAACGGAAGCTATTCGAGTGCTTGCAAGGCCATGCGGAGTTCAGCGTAAAGAAGCTTGAGAACCAAGTGCAACAGTTTCTCATCTTGGCTAATAGTGATCCCTCCTCATCCTCATCAGTAGTGGAAAAGAAGGGCTTTGCAGACTTTCGTGTAAGACTCCAAGACCTTACAAATATGACAGCGACCTATTTTAAGAACCTGGTTAGGGCATTAGAGCATGGGCTTCCAGAGACATGCTGCGAAGGAGCTAGTTGGTCTTGTGAGCGCTGTACTTATATCAATGCAATGTCTTCCGTTAAATGTGGGATGTGTACTGCACACATTCCAGAGGGCGCCGGTTACTGGTCTTGTGACAAGTGCACCTATTTCAATCCTGCGTCCAGCACCGGCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
IBR zf-RanBP

Protein Analysis

481

Amino Acids

54.74

Weight (kDa)

5.16

Isoelectric Point (pI)

55.52

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
IBR PF01485 199 - 265 2.6e-13 IBR domain, a half RING-finger domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0018171)

Species Orthologous Gene IDs
rosa_chinensis RchiOBHm_Chr3g0460891
rosa_laevigata RLG00000024969
rosa_multiflora Rmu_sc0008258.1_g000008 Rmu_sc0021377.1_g000001
rosa_roxburghii Rroxscaffold_6G00419050
rosa_rugosa Rorug03G0047000
rosa_samantha Rh3AG104300 Rh3BG108000 Rh3CG109700 Rh3DG109800
rosa_wichuraiana Rw3G008880

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 594
Acc36I ACCTGC 1 cut(s) 979
AccB1I GGYRCC 1 cut(s) 1385
AccII CGCG 1 cut(s) 309
AccIII TCCGGA 1 cut(s) 664
AciI CCGC 4 cut(s) 307, 423, 678, 1088
AclWI GGATC 1 cut(s) 1145
AcoI YGGCCR 1 cut(s) 567
AcuI CTGAAG 2 cut(s) 158, 170
AcyI GRCGYC 1 cut(s) 1386
AfaI GTAC 5 cut(s) 602, 709, 1013, 1324, 1366
AfeI AGCGCT 2 cut(s) 848, 1319
AfiI CCNNNNNNNGG 5 cut(s) 282, 437, 684, 1258, 1395
AgsI TTSAA 5 cut(s) 83, 328, 887, 980, 1423
AhlI ACTAGT 1 cut(s) 710
AjnI CCWGG 1 cut(s) 1251
AluBI AGCT 6 cut(s) 317, 416, 881, 1063, 1108, 1301
AluI AGCT 6 cut(s) 317, 416, 881, 1063, 1108, 1301
Alw21I GWGCWC 1 cut(s) 1415
Alw26I GTCTC 2 cut(s) 730, 1277
Alw44I GTGCAC 1 cut(s) 1411
AlwI GGATC 1 cut(s) 1145
Aor13HI TCCGGA 1 cut(s) 664
Aor51HI AGCGCT 2 cut(s) 848, 1319
AoxI GGCC 2 cut(s) 567, 1081
ApaLI GTGCAC 1 cut(s) 1411
ApeKI GCWGC 2 cut(s) 1045, 1290
AseI ATTAAT 1 cut(s) 470
Asp700I GAANNNNTTC 1 cut(s) 1064
AspLEI GCGC 4 cut(s) 849, 1045, 1320, 1388
AspS9I GGNCC 4 cut(s) 13, 261, 485, 539
AsuHPI GGTGA 4 cut(s) 122, 265, 365, 913
AsuII TTCGAA 1 cut(s) 818
AvaII GGWCC 4 cut(s) 13, 261, 485, 539
BaeGI GKGCMC 2 cut(s) 939, 1415
BalI TGGCCA 1 cut(s) 569
BanI GGYRCC 1 cut(s) 1385
BauI CACGAG 2 cut(s) 727, 938
BbsI GAAGAC 2 cut(s) 911, 1335
Bbv12I GWGCWC 1 cut(s) 1415
BbvI GCAGC 2 cut(s) 1057, 1277
BccI CCATC 2 cut(s) 24, 993
BcgI CGANNNNNNTGC 2 cut(s) 509, 543
BciT130I CCWGG 1 cut(s) 1253
BclI TGATCA 2 cut(s) 645, 1033
BcoDI GTCTC 2 cut(s) 730, 1277
BcuI ACTAGT 1 cut(s) 710
BfaI CTAG 3 cut(s) 705, 711, 1302
BfmI CTRYAG 1 cut(s) 801
BfoI RGCGCY 3 cut(s) 850, 1321, 1389
BfuAI ACCTGC 1 cut(s) 979
BglII AGATCT 2 cut(s) 358, 620
BisI GCNGC 2 cut(s) 1046, 1291
BlsI GCNGC 2 cut(s) 1047, 1292
BmcAI AGTACT 1 cut(s) 709
Bme1390I CCNGG 1 cut(s) 1253
Bme18I GGWCC 4 cut(s) 13, 261, 485, 539
BmgT120I GGNCC 4 cut(s) 13, 261, 485, 539
BmiI GGNNCC 3 cut(s) 122, 486, 1387
BmrFI CCNGG 1 cut(s) 1253
BmsI GCATC 5 cut(s) 217, 268, 682, 925, 1057
BpiI GAAGAC 2 cut(s) 911, 1335
Bpu14I TTCGAA 1 cut(s) 818
BpuEI CTTGAG 1 cut(s) 1130
BsaHI GRCGYC 1 cut(s) 1386
BsaJI CCNNGG 2 cut(s) 786, 859
BsaWI WCCGGW 1 cut(s) 664
Bsc4I CCNNNNNNNGG 5 cut(s) 282, 437, 684, 1258, 1395
Bse118I RCCGGY 3 cut(s) 350, 1388, 1439
Bse1I ACTGG 2 cut(s) 345, 1400
Bse3DI GCAATG 1 cut(s) 1344
BseAI TCCGGA 1 cut(s) 664
BseBI CCWGG 1 cut(s) 1253
BseDI CCNNGG 2 cut(s) 786, 859
BseGI GGATG 6 cut(s) 187, 427, 697, 1048, 1160, 1365
BseLI CCNNNNNNNGG 5 cut(s) 282, 437, 684, 1258, 1395
BseMI GCAATG 1 cut(s) 1344
BseMII CTCAG 1 cut(s) 426
BseNI ACTGG 2 cut(s) 345, 1400
BseRI GAGGAG 5 cut(s) 494, 779, 782, 785, 1147
BseSI GKGCMC 2 cut(s) 939, 1415
BseXI GCAGC 2 cut(s) 1057, 1277
BsgI GTGCAG 2 cut(s) 549, 1353
Bsh1236I CGCG 1 cut(s) 309
Bsh1285I CGRYCG 1 cut(s) 912
BshFI GGCC 2 cut(s) 569, 1083
BshNI GGYRCC 1 cut(s) 1385
BsiEI CGRYCG 1 cut(s) 912
BsiHKAI GWGCWC 1 cut(s) 1415
BsiSI CCGG 5 cut(s) 42, 351, 665, 1389, 1440
BslFI GGGAC 1 cut(s) 768
BslI CCNNNNNNNGG 5 cut(s) 282, 437, 684, 1258, 1395
BsmAI GTCTC 2 cut(s) 730, 1277
BsmFI GGGAC 1 cut(s) 768
BsmI GAATGC 1 cut(s) 986
BsnI GGCC 2 cut(s) 569, 1083
Bsp119I TTCGAA 1 cut(s) 818
Bsp1286I GDGCHC 2 cut(s) 939, 1415
Bsp13I TCCGGA 1 cut(s) 664
Bsp143I GATC 6 cut(s) 358, 620, 634, 645, 1033, 1150
BspACI CCGC 4 cut(s) 307, 423, 678, 1088
BspANI GGCC 2 cut(s) 569, 1083
BspCNI CTCAG 1 cut(s) 425
BspEI TCCGGA 1 cut(s) 664
BspFNI CGCG 1 cut(s) 309
BspHI TCATGA 1 cut(s) 1036
BspLI GGNNCC 3 cut(s) 122, 486, 1387
BspMAI CTGCAG 1 cut(s) 805
BspMI ACCTGC 1 cut(s) 979
BspPI GGATC 1 cut(s) 1145
BspT104I TTCGAA 1 cut(s) 818
BspT107I GGYRCC 1 cut(s) 1385
BsrDI GCAATG 1 cut(s) 1344
BsrFI RCCGGY 3 cut(s) 350, 1388, 1439
BsrI ACTGG 2 cut(s) 345, 1400
BssAI RCCGGY 3 cut(s) 350, 1388, 1439
BssECI CCNNGG 2 cut(s) 786, 859
BssMI GATC 6 cut(s) 358, 620, 634, 645, 1033, 1150
BssNI GRCGYC 1 cut(s) 1386
BssSI CACGAG 2 cut(s) 727, 938
Bst2BI CACGAG 2 cut(s) 727, 938
Bst2UI CCWGG 1 cut(s) 1253
Bst4CI ACNGT 4 cut(s) 32, 761, 913, 1128
BstACI GRCGYC 1 cut(s) 1386
BstAPI GCANNNNNTGC 3 cut(s) 287, 375, 994
BstBI TTCGAA 1 cut(s) 818
BstC8I GCNNGC 6 cut(s) 232, 319, 851, 964, 986, 1076
BstDEI CTNAG 1 cut(s) 412
BstDSI CCRYGG 2 cut(s) 786, 859
BstF5I GGATG 6 cut(s) 187, 427, 697, 1048, 1160, 1365
BstFNI CGCG 1 cut(s) 309
BstH2I RGCGCY 3 cut(s) 850, 1321, 1389
BstHHI GCGC 4 cut(s) 849, 1045, 1320, 1388
BstKTI GATC 6 cut(s) 361, 623, 637, 648, 1036, 1153
BstMAI GTCTC 2 cut(s) 730, 1277
BstMBI GATC 6 cut(s) 358, 620, 634, 645, 1033, 1150
BstMCI CGRYCG 1 cut(s) 912
BstMWI GCNNNNNNNGC 5 cut(s) 287, 375, 844, 994, 1080
BstNI CCWGG 1 cut(s) 1253
BstNSI RCATGY 4 cut(s) 234, 495, 966, 1290
BstSCI CCNGG 1 cut(s) 1251
BstSFI CTRYAG 1 cut(s) 801
BstSLI GKGCMC 2 cut(s) 939, 1415
BstUI CGCG 1 cut(s) 309
BstV1I GCAGC 2 cut(s) 1057, 1277
BstV2I GAAGAC 2 cut(s) 911, 1335
BstX2I RGATCY 2 cut(s) 358, 620
BstYI RGATCY 2 cut(s) 358, 620
BsuRI GGCC 2 cut(s) 569, 1083
BtgI CCRYGG 2 cut(s) 786, 859
BtgZI GCGATG 1 cut(s) 1012
BtsCI GGATG 6 cut(s) 187, 427, 697, 1048, 1160, 1365
BtsI GCAGTG 1 cut(s) 537
BtsIMutI CAGTG 1 cut(s) 537
BveI ACCTGC 1 cut(s) 979
Cac8I GCNNGC 6 cut(s) 232, 319, 851, 964, 986, 1076
CciI TCATGA 1 cut(s) 1036
CfoI GCGC 4 cut(s) 849, 1045, 1320, 1388
Cfr10I RCCGGY 3 cut(s) 350, 1388, 1439
Cfr13I GGNCC 4 cut(s) 13, 261, 485, 539
CseI GACGC 2 cut(s) 186, 1419
CsiI ACCWGGT 1 cut(s) 1251
Csp6I GTAC 5 cut(s) 601, 708, 1012, 1323, 1365
CviQI GTAC 5 cut(s) 601, 708, 1012, 1323, 1365
DdeI CTNAG 1 cut(s) 412
DinI GGCGCC 1 cut(s) 1387
DpnI GATC 6 cut(s) 360, 622, 636, 647, 1035, 1152
DpnII GATC 6 cut(s) 358, 620, 634, 645, 1033, 1150
EaeI YGGCCR 1 cut(s) 567
Eco47I GGWCC 4 cut(s) 13, 261, 485, 539
Eco47III AGCGCT 2 cut(s) 848, 1319
Eco57I CTGAAG 2 cut(s) 158, 170
EcoO109I RGGNCCY 1 cut(s) 485
EcoRII CCWGG 1 cut(s) 1251
EcoT22I ATGCAT 2 cut(s) 232, 283
EgeI GGCGCC 1 cut(s) 1387
EheI GGCGCC 1 cut(s) 1387
FalI AAGNNNNNCTT 2 cut(s) 1172, 1204
FaqI GGGAC 1 cut(s) 768
FbaI TGATCA 2 cut(s) 645, 1033
Fnu4HI GCNGC 2 cut(s) 1046, 1291
FokI GGATG 6 cut(s) 194, 414, 704, 1035, 1147, 1372
Fsp4HI GCNGC 2 cut(s) 1046, 1291
FspBI CTAG 3 cut(s) 705, 711, 1302
GlaI GCGC 4 cut(s) 848, 1044, 1319, 1387
GluI GCNGC 2 cut(s) 1046, 1291
HaeII RGCGCY 3 cut(s) 850, 1321, 1389
HaeIII GGCC 2 cut(s) 569, 1083
HapII CCGG 5 cut(s) 42, 351, 665, 1389, 1440
HgaI GACGC 2 cut(s) 186, 1419
HhaI GCGC 4 cut(s) 849, 1045, 1320, 1388
Hin1I GRCGYC 1 cut(s) 1386
Hin6I GCGC 4 cut(s) 847, 1043, 1318, 1386
HinP1I GCGC 4 cut(s) 847, 1043, 1318, 1386
HindIII AAGCTT 1 cut(s) 1106
HinfI GANTC 3 cut(s) 775, 830, 1209
HpaII CCGG 5 cut(s) 42, 351, 665, 1389, 1440
HphI GGTGA 4 cut(s) 122, 265, 365, 913
Hpy166II GTNNAC 5 cut(s) 201, 790, 1012, 1365, 1413
Hpy188I TCNGA 5 cut(s) 21, 38, 100, 150, 226
Hpy188III TCNNGA 8 cut(s) 176, 512, 665, 884, 920, 1037, 1280, 1379
Hpy8I GTNNAC 5 cut(s) 201, 790, 1012, 1365, 1413
HpyAV CCTTC 4 cut(s) 254, 926, 1177, 1289
HpyCH4III ACNGT 4 cut(s) 32, 761, 913, 1128
HpyCH4IV ACGT 1 cut(s) 558
HpyF10VI GCNNNNNNNGC 5 cut(s) 287, 375, 844, 994, 1080
HpyF3I CTNAG 1 cut(s) 412
HpySE526I ACGT 1 cut(s) 558
Hsp92I GRCGYC 1 cut(s) 1386
HspAI GCGC 4 cut(s) 847, 1043, 1318, 1386
KasI GGCGCC 1 cut(s) 1385
Kpn2I TCCGGA 1 cut(s) 664
Ksp22I TGATCA 2 cut(s) 645, 1033
Kzo9I GATC 6 cut(s) 358, 620, 634, 645, 1033, 1150
LmnI GCTCC 3 cut(s) 246, 667, 1298
Lsp1109I GCAGC 2 cut(s) 1057, 1277
LweI GCATC 5 cut(s) 217, 268, 682, 925, 1057
MabI ACCWGGT 1 cut(s) 1251
MaeI CTAG 3 cut(s) 705, 711, 1302
MaeII ACGT 1 cut(s) 558
MaeIII GTNAC 4 cut(s) 455, 671, 1391, 1403
MalI GATC 6 cut(s) 360, 622, 636, 647, 1035, 1152
MboI GATC 6 cut(s) 358, 620, 634, 645, 1033, 1150
MboII GAAGA 8 cut(s) 71, 170, 340, 368, 593, 644, 916, 1335
MflI RGATCY 2 cut(s) 358, 620
MhlI GDGCHC 2 cut(s) 939, 1415
MlsI TGGCCA 1 cut(s) 569
MluCI AATT 4 cut(s) 136, 253, 467, 923
MluNI TGGCCA 1 cut(s) 569
Mly113I GGCGCC 1 cut(s) 1386
MlyI GAGTC 1 cut(s) 1203
MmeI TCCRAC 3 cut(s) 517, 669, 924
Mox20I TGGCCA 1 cut(s) 569
Mph1103I ATGCAT 2 cut(s) 232, 283
MroI TCCGGA 1 cut(s) 664
MroXI GAANNNNTTC 1 cut(s) 1064
MscI TGGCCA 1 cut(s) 569
MseI TTAA 3 cut(s) 470, 1245, 1350
Msp20I TGGCCA 1 cut(s) 569
MspI CCGG 5 cut(s) 42, 351, 665, 1389, 1440
MspR9I CCNGG 1 cut(s) 1253
Mva1269I GAATGC 1 cut(s) 986
MvaI CCWGG 1 cut(s) 1253
MvnI CGCG 1 cut(s) 309
MwoI GCNNNNNNNGC 5 cut(s) 287, 375, 844, 994, 1080
NarI GGCGCC 1 cut(s) 1386
NdeII GATC 6 cut(s) 358, 620, 634, 645, 1033, 1150
NlaIV GGNNCC 3 cut(s) 122, 486, 1387
NmuCI GTSAC 1 cut(s) 1403
NsiI ATGCAT 2 cut(s) 232, 283
NspI RCATGY 4 cut(s) 234, 495, 966, 1290
NspV TTCGAA 1 cut(s) 818
PaeI GCATGC 2 cut(s) 234, 966
PagI TCATGA 1 cut(s) 1036
PctI GAATGC 1 cut(s) 986
PdmI GAANNNNTTC 1 cut(s) 1064
PfeI GAWTC 2 cut(s) 775, 830
PkrI GCNGC 2 cut(s) 1047, 1292
PleI GAGTC 1 cut(s) 1203
PluTI GGCGCC 1 cut(s) 1389
PpsI GAGTC 1 cut(s) 1203
PpuMI RGGWCCY 1 cut(s) 485
PshBI ATTAAT 1 cut(s) 470
PsiI TTATAA 1 cut(s) 594
Psp5II RGGWCCY 1 cut(s) 485
Psp6I CCWGG 1 cut(s) 1251
PspGI CCWGG 1 cut(s) 1251
PspN4I GGNNCC 3 cut(s) 122, 486, 1387
PspPI GGNCC 4 cut(s) 13, 261, 485, 539
PspPPI RGGWCCY 1 cut(s) 485
PstI CTGCAG 1 cut(s) 805
PsuI RGATCY 2 cut(s) 358, 620
RsaI GTAC 5 cut(s) 602, 709, 1013, 1324, 1366
RsaNI GTAC 5 cut(s) 601, 708, 1012, 1323, 1365
SaqAI TTAA 3 cut(s) 470, 1245, 1350
SatI GCNGC 2 cut(s) 1046, 1291
Sau3AI GATC 6 cut(s) 358, 620, 634, 645, 1033, 1150
Sau96I GGNCC 4 cut(s) 13, 261, 485, 539
ScaI AGTACT 1 cut(s) 709
SchI GAGTC 1 cut(s) 1203
ScrFI CCNGG 1 cut(s) 1253
SduI GDGCHC 2 cut(s) 939, 1415
SexAI ACCWGGT 1 cut(s) 1251
SfaNI GCATC 5 cut(s) 217, 268, 682, 925, 1057
SfcI CTRYAG 1 cut(s) 801
SfoI GGCGCC 1 cut(s) 1387
SfuI TTCGAA 1 cut(s) 818
SinI GGWCC 4 cut(s) 13, 261, 485, 539
SmlI CTYRAG 1 cut(s) 1109
SmoI CTYRAG 1 cut(s) 1109
SpeI ACTAGT 1 cut(s) 710
SphI GCATGC 2 cut(s) 234, 966
Sse9I AATT 4 cut(s) 136, 253, 467, 923
SsiI CCGC 4 cut(s) 307, 423, 678, 1088
SspDI GGCGCC 1 cut(s) 1385
SspMI CTAG 3 cut(s) 705, 711, 1302
StyD4I CCNGG 1 cut(s) 1251
TaaI ACNGT 4 cut(s) 32, 761, 913, 1128
TaiI ACGT 1 cut(s) 561
TaqI TCGA 4 cut(s) 87, 251, 818, 1068
TaqII GACCGA 1 cut(s) 278
TasI AATT 4 cut(s) 136, 253, 467, 923
TatI WGTACW 3 cut(s) 707, 1322, 1364
TfiI GAWTC 2 cut(s) 775, 830
Tru1I TTAA 3 cut(s) 470, 1245, 1350
Tru9I TTAA 3 cut(s) 470, 1245, 1350
TscAI CASTG 1 cut(s) 537
TseFI GTSAC 1 cut(s) 1403
TseI GCWGC 2 cut(s) 1045, 1290
Tsp45I GTSAC 1 cut(s) 1403
TspDTI ATGAA 4 cut(s) 72, 309, 378, 600
TspGWI ACGGA 4 cut(s) 823, 876, 1072, 1336
TspRI CASTG 1 cut(s) 537
VneI GTGCAC 1 cut(s) 1411
VpaK11BI GGWCC 4 cut(s) 13, 261, 485, 539
VspI ATTAAT 1 cut(s) 470
XceI RCATGY 4 cut(s) 234, 495, 966, 1290
XmnI GAANNNNTTC 1 cut(s) 1064
XspI CTAG 3 cut(s) 705, 711, 1302
ZrmI AGTACT 1 cut(s) 709
Zsp2I ATGCAT 2 cut(s) 232, 283
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.