Rmu_sc0008258.1_g000008

E3 ubiquitin-protein ligase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0008258.1
OG0018171
Physical Location & Seq
Reverse (-)
27631 .. 29097
1467 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0008258.1_g000008.1.cds

Sequence Viewer

Length: 1467 bp
atggaccatcagaacgatgacagtttcagagccggagagcgtatagatgaagaagcatattatgattgccttgaagtcgatgacaaatccgataagttgggtgagatttggttcccacagaaaaagaattatactattctgaagcaagaagaagttgccaaacttcaggaggatgatattgcaaaagcgtccacttttctttcggtatcaaaatctgatgcatgcttactgctccttcactcgaattggaggaccgataatctcaccgatgcatggtttgctgatgaaaataaaatccgcgaaaaagctggcttgttgaagaaacccctactggataatgccggtgaagatctaataagcagtagttgccgactatgttcatatagtaatagtagtaatatctcagcttcttgcggtcatcctttttgtggagattgctggacaggttacataacaaaattaatcaatgaggagggtcctgcatgtttggttttgagatgtcctgaacccgattgtagtgcagtggttggacctgatttagtccaaaacgtaatgttggccacaaaccaagaagatgaatgttataagaggtacaagcattacctattgagatcttatttagaagatcacaagttgatcaagtggtgtcctgctccggattgtaactccgctattaggttggatgctggtggttttagtactagttgctatcatgtctcgtgtgtttgctctcgtagttattgctggaactgtgaggaggaggagaatcatcgtcccgtggactgccaaactgcagggaagtggatttcgaatatattagaatccgtgcattaccagcgctggcaaaaccacggaaaatcaaaggacaaagctcttgaagtatttgacgaggtgaagacgacagtgcatcaagaattattagaagggcacgagttggattttttgttagaggcaagcaaacagatgattgaatgcaggcaggtattgcgatggattgttgtgtacgattactacaacatgagtgatcatgaggcgcagcatcctaaacggaagctattcgagtgcttgcaaggccatgcggagttcagcgtgaagaagcttgagaaccaagtgcaacagtttctcatcttggctaatagtgatccctcctcatcctcatcagtagtggaaaagatgggctttgcagactttcgtgtaagactccaagaccttacaaatatgacagcgacctattttaagaacctggttagggcattagagcaagggcttccagagacatgctgcgaaggagctagttggtcttgtgagcgctgtacttatatcaatgcaatgtctgccgttaaatgtgggatgtgtactgcacacgttccagagggcgccggttattggtcttgtgataagtgcacctatttcaatcctgcgtccagcaccagctgtttgatgtgttcattggcagagtgctgctga
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
IBR zf-RanBP

Protein Analysis

488

Amino Acids

55.44

Weight (kDa)

5.11

Isoelectric Point (pI)

56.94

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0018171)

Species Orthologous Gene IDs
rosa_chinensis RchiOBHm_Chr3g0460891
rosa_laevigata RLG00000024969
rosa_multiflora Rmu_sc0008258.1_g000008 Rmu_sc0021377.1_g000001
rosa_roxburghii Rroxscaffold_6G00419050
rosa_rugosa Rorug03G0047000
rosa_samantha Rh3AG104300 Rh3BG108000 Rh3CG109700 Rh3DG109800
rosa_wichuraiana Rw3G008880

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 585
Acc36I ACCTGC 1 cut(s) 970
AccB1I GGYRCC 1 cut(s) 1376
AccII CGCG 1 cut(s) 300
AccIII TCCGGA 1 cut(s) 655
AciI CCGC 4 cut(s) 298, 414, 669, 1079
AclWI GGATC 1 cut(s) 1136
AcoI YGGCCR 1 cut(s) 558
AcuI CTGAAG 2 cut(s) 149, 161
AcyI GRCGYC 1 cut(s) 1377
AfaI GTAC 5 cut(s) 593, 700, 1004, 1315, 1357
AfeI AGCGCT 2 cut(s) 839, 1310
AfiI CCNNNNNNNGG 5 cut(s) 273, 428, 675, 1249, 1386
AflIII ACRYGT 1 cut(s) 1363
AgsI TTSAA 5 cut(s) 74, 319, 878, 971, 1414
AhlI ACTAGT 1 cut(s) 701
AjnI CCWGG 1 cut(s) 1242
AluBI AGCT 7 cut(s) 308, 407, 872, 1054, 1099, 1292, 1434
AluI AGCT 7 cut(s) 308, 407, 872, 1054, 1099, 1292, 1434
Alw21I GWGCWC 1 cut(s) 1406
Alw26I GTCTC 2 cut(s) 721, 1268
Alw44I GTGCAC 1 cut(s) 1402
AlwI GGATC 1 cut(s) 1136
Aor13HI TCCGGA 1 cut(s) 655
Aor51HI AGCGCT 2 cut(s) 839, 1310
AoxI GGCC 2 cut(s) 558, 1072
ApaLI GTGCAC 1 cut(s) 1402
ApeKI GCWGC 3 cut(s) 1036, 1281, 1461
AseI ATTAAT 1 cut(s) 461
Asp700I GAANNNNTTC 1 cut(s) 1055
AspLEI GCGC 4 cut(s) 840, 1036, 1311, 1379
AspS9I GGNCC 4 cut(s) 4, 252, 476, 530
AsuHPI GGTGA 4 cut(s) 113, 256, 356, 904
AsuII TTCGAA 1 cut(s) 809
AvaII GGWCC 4 cut(s) 4, 252, 476, 530
BaeGI GKGCMC 2 cut(s) 930, 1406
BalI TGGCCA 1 cut(s) 560
BanI GGYRCC 1 cut(s) 1376
BauI CACGAG 2 cut(s) 718, 929
BbsI GAAGAC 1 cut(s) 902
Bbv12I GWGCWC 1 cut(s) 1406
BbvI GCAGC 3 cut(s) 1048, 1268, 1448
BccI CCATC 3 cut(s) 15, 984, 1168
BceAI ACGGC 1 cut(s) 1322
BcgI CGANNNNNNTGC 2 cut(s) 500, 534
BciT130I CCWGG 1 cut(s) 1244
BclI TGATCA 2 cut(s) 636, 1024
BcoDI GTCTC 2 cut(s) 721, 1268
BcuI ACTAGT 1 cut(s) 701
BfaI CTAG 2 cut(s) 702, 1293
BfmI CTRYAG 1 cut(s) 792
BfoI RGCGCY 3 cut(s) 841, 1312, 1380
BfuAI ACCTGC 1 cut(s) 970
BglII AGATCT 2 cut(s) 349, 611
BisI GCNGC 3 cut(s) 1037, 1282, 1462
BlsI GCNGC 3 cut(s) 1038, 1283, 1463
BmcAI AGTACT 1 cut(s) 700
Bme1390I CCNGG 1 cut(s) 1244
Bme18I GGWCC 4 cut(s) 4, 252, 476, 530
BmgT120I GGNCC 4 cut(s) 4, 252, 476, 530
BmiI GGNNCC 3 cut(s) 113, 477, 1378
BmrFI CCNGG 1 cut(s) 1244
BmsI GCATC 5 cut(s) 208, 259, 673, 916, 1048
BpiI GAAGAC 1 cut(s) 902
Bpu14I TTCGAA 1 cut(s) 809
BpuEI CTTGAG 1 cut(s) 1121
BsaHI GRCGYC 1 cut(s) 1377
BsaJI CCNNGG 2 cut(s) 777, 850
BsaWI WCCGGW 1 cut(s) 655
Bsc4I CCNNNNNNNGG 5 cut(s) 273, 428, 675, 1249, 1386
Bse118I RCCGGY 2 cut(s) 341, 1379
Bse1I ACTGG 1 cut(s) 336
Bse3DI GCAATG 1 cut(s) 1335
BseAI TCCGGA 1 cut(s) 655
BseBI CCWGG 1 cut(s) 1244
BseDI CCNNGG 2 cut(s) 777, 850
BseGI GGATG 6 cut(s) 178, 418, 688, 1039, 1151, 1356
BseLI CCNNNNNNNGG 5 cut(s) 273, 428, 675, 1249, 1386
BseMI GCAATG 1 cut(s) 1335
BseMII CTCAG 1 cut(s) 417
BseNI ACTGG 1 cut(s) 336
BseRI GAGGAG 5 cut(s) 485, 770, 773, 776, 1138
BseSI GKGCMC 2 cut(s) 930, 1406
BseXI GCAGC 3 cut(s) 1048, 1268, 1448
BsgI GTGCAG 2 cut(s) 540, 1344
Bsh1236I CGCG 1 cut(s) 300
BshFI GGCC 2 cut(s) 560, 1074
BshNI GGYRCC 1 cut(s) 1376
BsiHKAI GWGCWC 1 cut(s) 1406
BsiSI CCGG 4 cut(s) 33, 342, 656, 1380
BslFI GGGAC 1 cut(s) 759
BslI CCNNNNNNNGG 5 cut(s) 273, 428, 675, 1249, 1386
BsmAI GTCTC 2 cut(s) 721, 1268
BsmFI GGGAC 1 cut(s) 759
BsmI GAATGC 1 cut(s) 977
BsnI GGCC 2 cut(s) 560, 1074
Bsp119I TTCGAA 1 cut(s) 809
Bsp1286I GDGCHC 2 cut(s) 930, 1406
Bsp13I TCCGGA 1 cut(s) 655
Bsp143I GATC 6 cut(s) 349, 611, 625, 636, 1024, 1141
BspACI CCGC 4 cut(s) 298, 414, 669, 1079
BspANI GGCC 2 cut(s) 560, 1074
BspCNI CTCAG 1 cut(s) 416
BspEI TCCGGA 1 cut(s) 655
BspFNI CGCG 1 cut(s) 300
BspHI TCATGA 1 cut(s) 1027
BspLI GGNNCC 3 cut(s) 113, 477, 1378
BspMAI CTGCAG 1 cut(s) 796
BspMI ACCTGC 1 cut(s) 970
BspPI GGATC 1 cut(s) 1136
BspT104I TTCGAA 1 cut(s) 809
BspT107I GGYRCC 1 cut(s) 1376
BsrDI GCAATG 1 cut(s) 1335
BsrFI RCCGGY 2 cut(s) 341, 1379
BsrI ACTGG 1 cut(s) 336
BssAI RCCGGY 2 cut(s) 341, 1379
BssECI CCNNGG 2 cut(s) 777, 850
BssMI GATC 6 cut(s) 349, 611, 625, 636, 1024, 1141
BssNI GRCGYC 1 cut(s) 1377
BssSI CACGAG 2 cut(s) 718, 929
Bst2BI CACGAG 2 cut(s) 718, 929
Bst2UI CCWGG 1 cut(s) 1244
Bst4CI ACNGT 4 cut(s) 23, 752, 904, 1119
BstACI GRCGYC 1 cut(s) 1377
BstAPI GCANNNNNTGC 4 cut(s) 278, 366, 985, 1334
BstBI TTCGAA 1 cut(s) 809
BstC8I GCNNGC 6 cut(s) 223, 310, 842, 955, 977, 1067
BstDEI CTNAG 1 cut(s) 403
BstDSI CCRYGG 2 cut(s) 777, 850
BstF5I GGATG 6 cut(s) 178, 418, 688, 1039, 1151, 1356
BstFNI CGCG 1 cut(s) 300
BstH2I RGCGCY 3 cut(s) 841, 1312, 1380
BstHHI GCGC 4 cut(s) 840, 1036, 1311, 1379
BstKTI GATC 6 cut(s) 352, 614, 628, 639, 1027, 1144
BstMAI GTCTC 2 cut(s) 721, 1268
BstMBI GATC 6 cut(s) 349, 611, 625, 636, 1024, 1141
BstMWI GCNNNNNNNGC 6 cut(s) 278, 366, 835, 985, 1071, 1334
BstNI CCWGG 1 cut(s) 1244
BstNSI RCATGY 3 cut(s) 225, 486, 1281
BstSCI CCNGG 1 cut(s) 1242
BstSFI CTRYAG 1 cut(s) 792
BstSLI GKGCMC 2 cut(s) 930, 1406
BstUI CGCG 1 cut(s) 300
BstV1I GCAGC 3 cut(s) 1048, 1268, 1448
BstV2I GAAGAC 1 cut(s) 902
BstX2I RGATCY 2 cut(s) 349, 611
BstYI RGATCY 2 cut(s) 349, 611
BsuRI GGCC 2 cut(s) 560, 1074
BtgI CCRYGG 2 cut(s) 777, 850
BtgZI GCGATG 1 cut(s) 1003
BtsCI GGATG 6 cut(s) 178, 418, 688, 1039, 1151, 1356
BtsI GCAGTG 1 cut(s) 528
BtsIMutI CAGTG 2 cut(s) 528, 909
BveI ACCTGC 1 cut(s) 970
Cac8I GCNNGC 6 cut(s) 223, 310, 842, 955, 977, 1067
CciI TCATGA 1 cut(s) 1027
CfoI GCGC 4 cut(s) 840, 1036, 1311, 1379
Cfr10I RCCGGY 2 cut(s) 341, 1379
Cfr13I GGNCC 4 cut(s) 4, 252, 476, 530
CseI GACGC 2 cut(s) 177, 1410
CsiI ACCWGGT 1 cut(s) 1242
Csp6I GTAC 5 cut(s) 592, 699, 1003, 1314, 1356
CviAII CATG 8 cut(s) 222, 273, 483, 713, 1018, 1028, 1076, 1278
CviQI GTAC 5 cut(s) 592, 699, 1003, 1314, 1356
DdeI CTNAG 1 cut(s) 403
DinI GGCGCC 1 cut(s) 1378
DpnI GATC 6 cut(s) 351, 613, 627, 638, 1026, 1143
DpnII GATC 6 cut(s) 349, 611, 625, 636, 1024, 1141
EaeI YGGCCR 1 cut(s) 558
Eco47I GGWCC 4 cut(s) 4, 252, 476, 530
Eco47III AGCGCT 2 cut(s) 839, 1310
Eco57I CTGAAG 2 cut(s) 149, 161
EcoO109I RGGNCCY 1 cut(s) 476
EcoRII CCWGG 1 cut(s) 1242
EcoT22I ATGCAT 2 cut(s) 223, 274
EgeI GGCGCC 1 cut(s) 1378
EheI GGCGCC 1 cut(s) 1378
FaeI CATG 8 cut(s) 225, 276, 486, 716, 1021, 1031, 1079, 1281
FalI AAGNNNNNCTT 2 cut(s) 1163, 1195
FaqI GGGAC 1 cut(s) 759
FatI CATG 8 cut(s) 221, 272, 482, 712, 1017, 1027, 1075, 1277
FbaI TGATCA 2 cut(s) 636, 1024
Fnu4HI GCNGC 3 cut(s) 1037, 1282, 1462
FokI GGATG 6 cut(s) 185, 405, 695, 1026, 1138, 1363
Fsp4HI GCNGC 3 cut(s) 1037, 1282, 1462
FspBI CTAG 2 cut(s) 702, 1293
GlaI GCGC 4 cut(s) 839, 1035, 1310, 1378
GluI GCNGC 3 cut(s) 1037, 1282, 1462
HaeII RGCGCY 3 cut(s) 841, 1312, 1380
HaeIII GGCC 2 cut(s) 560, 1074
HapII CCGG 4 cut(s) 33, 342, 656, 1380
HgaI GACGC 2 cut(s) 177, 1410
HhaI GCGC 4 cut(s) 840, 1036, 1311, 1379
Hin1I GRCGYC 1 cut(s) 1377
Hin1II CATG 8 cut(s) 225, 276, 486, 716, 1021, 1031, 1079, 1281
Hin6I GCGC 4 cut(s) 838, 1034, 1309, 1377
HinP1I GCGC 4 cut(s) 838, 1034, 1309, 1377
HindIII AAGCTT 1 cut(s) 1097
HinfI GANTC 3 cut(s) 766, 821, 1200
HpaII CCGG 4 cut(s) 33, 342, 656, 1380
HphI GGTGA 4 cut(s) 113, 256, 356, 904
Hpy166II GTNNAC 5 cut(s) 192, 781, 1003, 1356, 1404
Hpy188I TCNGA 5 cut(s) 12, 29, 91, 141, 217
Hpy188III TCNNGA 8 cut(s) 167, 503, 656, 875, 911, 1028, 1271, 1370
Hpy8I GTNNAC 5 cut(s) 192, 781, 1003, 1356, 1404
HpyAV CCTTC 3 cut(s) 245, 917, 1280
HpyCH4III ACNGT 4 cut(s) 23, 752, 904, 1119
HpyCH4IV ACGT 2 cut(s) 549, 1365
HpyF10VI GCNNNNNNNGC 6 cut(s) 278, 366, 835, 985, 1071, 1334
HpyF3I CTNAG 1 cut(s) 403
HpySE526I ACGT 2 cut(s) 549, 1365
Hsp92I GRCGYC 1 cut(s) 1377
Hsp92II CATG 8 cut(s) 225, 276, 486, 716, 1021, 1031, 1079, 1281
HspAI GCGC 4 cut(s) 838, 1034, 1309, 1377
KasI GGCGCC 1 cut(s) 1376
Kpn2I TCCGGA 1 cut(s) 655
Ksp22I TGATCA 2 cut(s) 636, 1024
Kzo9I GATC 6 cut(s) 349, 611, 625, 636, 1024, 1141
LmnI GCTCC 3 cut(s) 237, 658, 1289
Lsp1109I GCAGC 3 cut(s) 1048, 1268, 1448
LweI GCATC 5 cut(s) 208, 259, 673, 916, 1048
MabI ACCWGGT 1 cut(s) 1242
MaeI CTAG 2 cut(s) 702, 1293
MaeII ACGT 2 cut(s) 549, 1365
MaeIII GTNAC 2 cut(s) 446, 662
MalI GATC 6 cut(s) 351, 613, 627, 638, 1026, 1143
MboI GATC 6 cut(s) 349, 611, 625, 636, 1024, 1141
MboII GAAGA 8 cut(s) 62, 161, 331, 359, 584, 635, 907, 1105
MflI RGATCY 2 cut(s) 349, 611
MhlI GDGCHC 2 cut(s) 930, 1406
MlsI TGGCCA 1 cut(s) 560
MluCI AATT 4 cut(s) 127, 244, 458, 914
MluNI TGGCCA 1 cut(s) 560
Mly113I GGCGCC 1 cut(s) 1377
MlyI GAGTC 1 cut(s) 1194
MmeI TCCRAC 3 cut(s) 508, 660, 915
Mox20I TGGCCA 1 cut(s) 560
Mph1103I ATGCAT 2 cut(s) 223, 274
MroI TCCGGA 1 cut(s) 655
MroXI GAANNNNTTC 1 cut(s) 1055
MscI TGGCCA 1 cut(s) 560
MseI TTAA 3 cut(s) 461, 1236, 1341
Msp20I TGGCCA 1 cut(s) 560
MspA1I CMGCKG 1 cut(s) 1434
MspI CCGG 4 cut(s) 33, 342, 656, 1380
MspR9I CCNGG 1 cut(s) 1244
Mva1269I GAATGC 1 cut(s) 977
MvaI CCWGG 1 cut(s) 1244
MvnI CGCG 1 cut(s) 300
MwoI GCNNNNNNNGC 6 cut(s) 278, 366, 835, 985, 1071, 1334
NarI GGCGCC 1 cut(s) 1377
NdeII GATC 6 cut(s) 349, 611, 625, 636, 1024, 1141
NlaIII CATG 8 cut(s) 225, 276, 486, 716, 1021, 1031, 1079, 1281
NlaIV GGNNCC 3 cut(s) 113, 477, 1378
NsiI ATGCAT 2 cut(s) 223, 274
NspI RCATGY 3 cut(s) 225, 486, 1281
NspV TTCGAA 1 cut(s) 809
PaeI GCATGC 1 cut(s) 225
PagI TCATGA 1 cut(s) 1027
PctI GAATGC 1 cut(s) 977
PdmI GAANNNNTTC 1 cut(s) 1055
PfeI GAWTC 2 cut(s) 766, 821
PkrI GCNGC 3 cut(s) 1038, 1283, 1463
PleI GAGTC 1 cut(s) 1194
PluTI GGCGCC 1 cut(s) 1380
PpsI GAGTC 1 cut(s) 1194
PpuMI RGGWCCY 1 cut(s) 476
PshBI ATTAAT 1 cut(s) 461
PsiI TTATAA 1 cut(s) 585
Psp5II RGGWCCY 1 cut(s) 476
Psp6I CCWGG 1 cut(s) 1242
PspGI CCWGG 1 cut(s) 1242
PspN4I GGNNCC 3 cut(s) 113, 477, 1378
PspPI GGNCC 4 cut(s) 4, 252, 476, 530
PspPPI RGGWCCY 1 cut(s) 476
PstI CTGCAG 1 cut(s) 796
PsuI RGATCY 2 cut(s) 349, 611
PvuII CAGCTG 1 cut(s) 1434
RsaI GTAC 5 cut(s) 593, 700, 1004, 1315, 1357
RsaNI GTAC 5 cut(s) 592, 699, 1003, 1314, 1356
SaqAI TTAA 3 cut(s) 461, 1236, 1341
SatI GCNGC 3 cut(s) 1037, 1282, 1462
Sau3AI GATC 6 cut(s) 349, 611, 625, 636, 1024, 1141
Sau96I GGNCC 4 cut(s) 4, 252, 476, 530
ScaI AGTACT 1 cut(s) 700
SchI GAGTC 1 cut(s) 1194
ScrFI CCNGG 1 cut(s) 1244
SduI GDGCHC 2 cut(s) 930, 1406
SexAI ACCWGGT 1 cut(s) 1242
SfaNI GCATC 5 cut(s) 208, 259, 673, 916, 1048
SfcI CTRYAG 1 cut(s) 792
SfoI GGCGCC 1 cut(s) 1378
SfuI TTCGAA 1 cut(s) 809
SinI GGWCC 4 cut(s) 4, 252, 476, 530
SmlI CTYRAG 1 cut(s) 1100
SmoI CTYRAG 1 cut(s) 1100
SpeI ACTAGT 1 cut(s) 701
SphI GCATGC 1 cut(s) 225
Sse9I AATT 4 cut(s) 127, 244, 458, 914
SsiI CCGC 4 cut(s) 298, 414, 669, 1079
SspDI GGCGCC 1 cut(s) 1376
SspMI CTAG 2 cut(s) 702, 1293
StyD4I CCNGG 1 cut(s) 1242
TaaI ACNGT 4 cut(s) 23, 752, 904, 1119
TaiI ACGT 2 cut(s) 552, 1368
TaqI TCGA 4 cut(s) 78, 242, 809, 1059
TaqII GACCGA 1 cut(s) 269
TasI AATT 4 cut(s) 127, 244, 458, 914
TatI WGTACW 3 cut(s) 698, 1313, 1355
TfiI GAWTC 2 cut(s) 766, 821
Tru1I TTAA 3 cut(s) 461, 1236, 1341
Tru9I TTAA 3 cut(s) 461, 1236, 1341
TscAI CASTG 2 cut(s) 528, 909
TseI GCWGC 3 cut(s) 1036, 1281, 1461
TspDTI ATGAA 5 cut(s) 63, 300, 369, 591, 1437
TspGWI ACGGA 3 cut(s) 814, 867, 1063
TspRI CASTG 2 cut(s) 528, 909
VneI GTGCAC 1 cut(s) 1402
VpaK11BI GGWCC 4 cut(s) 4, 252, 476, 530
VspI ATTAAT 1 cut(s) 461
XceI RCATGY 3 cut(s) 225, 486, 1281
XmnI GAANNNNTTC 1 cut(s) 1055
XspI CTAG 2 cut(s) 702, 1293
ZrmI AGTACT 1 cut(s) 700
Zsp2I ATGCAT 2 cut(s) 223, 274
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.