Rmu_sc0021377.1_g000001

E3 ubiquitin-protein ligase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0021377.1
OG0018171
Physical Location & Seq
Reverse (-)
1594 .. 2905
1312 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0021377.1_g000001.1.cds

Sequence Viewer

Length: 1065 bp
atggaccatcagaacgatgacagtttcagagccggagagcgtatagatgaagaagcatattatgattgccttgaagtcgatgacaaatccgttaagttgggtgagatttggttcccacagaaaaagaattatactattctgaagcaagaagaagttgccaaacttcaggaggatgatattgcacaagcgtccacttttctttcggtatcaaaatctgatgcatgcttactgctccttcactcgaattggaggaccgataatctcaccgatgcatggttggatgctggtggttttagtactagttgctatcatgtctcgtgtgtttgctctcgtagttattgctggaactgtgaggaggaggagaatcatcgtcccgtggactgccaaactgcagggaagtggatttcgaatatattagaatccgtgcattaccagcgctggcaaaaccacggaaaatcaaaggacaaagctcttgaagtatttgacgaggtgaagacgacagtgcatcaagaattattagaagggcacgagttggattttttgttagaggcaagcaaacagatgattgaatgcaggcaggtattgcgatggattgttgtgtacgattactacaacatgagtgatcatgaggcgcagcatcctaaacggaagctattcgagtgcttgcaaggccatgcggagttcagcgtgaagaagcttgagaaccaagtgcaacagtttctcatcttggctaatagtgatccctcctcatcctcatcagcagtggaaaagaagggctttgcagactttcgtgtaagactccaagaccttacaaatatgacagcgacctattttaagaacctggttagggcattagagcatgggcttccagagacatgctgcgaaggagctagttggtcttgtgagcgctgtacttatatcaatgcaatgtctgccgttaaatgtgggatgtgtactgcacacattccagagggcgccagttattggtcttgtgacaagtgcacctatttcaatcctgcgtccaccaccagctgtttgatgtgttcattggcagagtgctgctga
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
IBR zf-RanBP

Protein Analysis

354

Amino Acids

40.53

Weight (kDa)

5.1

Isoelectric Point (pI)

56.26

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0018171)

Species Orthologous Gene IDs
rosa_chinensis RchiOBHm_Chr3g0460891
rosa_laevigata RLG00000024969
rosa_multiflora Rmu_sc0008258.1_g000008 Rmu_sc0021377.1_g000001
rosa_roxburghii Rroxscaffold_6G00419050
rosa_rugosa Rorug03G0047000
rosa_samantha Rh3AG104300 Rh3BG108000 Rh3CG109700 Rh3DG109800
rosa_wichuraiana Rw3G008880

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 568
AccB1I GGYRCC 1 cut(s) 974
AccB7I CCANNNNNTGG 1 cut(s) 984
AciI CCGC 1 cut(s) 677
AclWI GGATC 1 cut(s) 734
AcuI CTGAAG 2 cut(s) 149, 161
AcyI GRCGYC 1 cut(s) 975
AfaI GTAC 4 cut(s) 298, 602, 913, 955
AfeI AGCGCT 2 cut(s) 437, 908
AfiI CCNNNNNNNGG 3 cut(s) 273, 847, 984
AgsI TTSAA 4 cut(s) 74, 476, 569, 1012
AhlI ACTAGT 1 cut(s) 299
AjnI CCWGG 1 cut(s) 840
AluBI AGCT 5 cut(s) 470, 652, 697, 890, 1032
AluI AGCT 5 cut(s) 470, 652, 697, 890, 1032
Alw21I GWGCWC 1 cut(s) 1004
Alw26I GTCTC 2 cut(s) 319, 866
Alw44I GTGCAC 1 cut(s) 1000
AlwI GGATC 1 cut(s) 734
Aor51HI AGCGCT 2 cut(s) 437, 908
AoxI GGCC 1 cut(s) 670
ApaLI GTGCAC 1 cut(s) 1000
ApeKI GCWGC 3 cut(s) 634, 879, 1059
Asp700I GAANNNNTTC 1 cut(s) 653
AspLEI GCGC 4 cut(s) 438, 634, 909, 977
AspS9I GGNCC 2 cut(s) 4, 252
AsuHPI GGTGA 3 cut(s) 113, 256, 502
AsuII TTCGAA 1 cut(s) 407
AvaII GGWCC 2 cut(s) 4, 252
BaeGI GKGCMC 2 cut(s) 528, 1004
BanI GGYRCC 1 cut(s) 974
BauI CACGAG 2 cut(s) 316, 527
BbsI GAAGAC 1 cut(s) 500
Bbv12I GWGCWC 1 cut(s) 1004
BbvI GCAGC 3 cut(s) 646, 866, 1046
BccI CCATC 2 cut(s) 15, 582
BceAI ACGGC 1 cut(s) 920
BciT130I CCWGG 1 cut(s) 842
BclI TGATCA 1 cut(s) 622
BcoDI GTCTC 2 cut(s) 319, 866
BcuI ACTAGT 1 cut(s) 299
BfaI CTAG 2 cut(s) 300, 891
BfmI CTRYAG 1 cut(s) 390
BfoI RGCGCY 3 cut(s) 439, 910, 978
BfuAI ACCTGC 1 cut(s) 568
BisI GCNGC 3 cut(s) 635, 880, 1060
BlsI GCNGC 3 cut(s) 636, 881, 1061
BmcAI AGTACT 1 cut(s) 298
Bme1390I CCNGG 1 cut(s) 842
Bme18I GGWCC 2 cut(s) 4, 252
BmgT120I GGNCC 2 cut(s) 4, 252
BmiI GGNNCC 2 cut(s) 113, 976
BmrFI CCNGG 1 cut(s) 842
BmsI GCATC 5 cut(s) 208, 259, 271, 514, 646
BpiI GAAGAC 1 cut(s) 500
Bpu14I TTCGAA 1 cut(s) 407
BpuEI CTTGAG 1 cut(s) 719
BsaHI GRCGYC 1 cut(s) 975
BsaJI CCNNGG 2 cut(s) 375, 448
Bsc4I CCNNNNNNNGG 3 cut(s) 273, 847, 984
Bse1I ACTGG 1 cut(s) 978
Bse3DI GCAATG 1 cut(s) 933
BseBI CCWGG 1 cut(s) 842
BseDI CCNNGG 2 cut(s) 375, 448
BseGI GGATG 5 cut(s) 178, 286, 637, 749, 954
BseLI CCNNNNNNNGG 3 cut(s) 273, 847, 984
BseMI GCAATG 1 cut(s) 933
BseNI ACTGG 1 cut(s) 978
BseRI GAGGAG 4 cut(s) 368, 371, 374, 736
BseSI GKGCMC 2 cut(s) 528, 1004
BseXI GCAGC 3 cut(s) 646, 866, 1046
BsgI GTGCAG 1 cut(s) 942
BshFI GGCC 1 cut(s) 672
BshNI GGYRCC 1 cut(s) 974
BsiHKAI GWGCWC 1 cut(s) 1004
BsiSI CCGG 1 cut(s) 33
BslFI GGGAC 1 cut(s) 357
BslI CCNNNNNNNGG 3 cut(s) 273, 847, 984
BsmAI GTCTC 2 cut(s) 319, 866
BsmFI GGGAC 1 cut(s) 357
BsmI GAATGC 1 cut(s) 575
BsnI GGCC 1 cut(s) 672
Bsp119I TTCGAA 1 cut(s) 407
Bsp1286I GDGCHC 2 cut(s) 528, 1004
Bsp143I GATC 2 cut(s) 622, 739
BspACI CCGC 1 cut(s) 677
BspANI GGCC 1 cut(s) 672
BspHI TCATGA 1 cut(s) 625
BspLI GGNNCC 2 cut(s) 113, 976
BspMAI CTGCAG 1 cut(s) 394
BspMI ACCTGC 1 cut(s) 568
BspPI GGATC 1 cut(s) 734
BspT104I TTCGAA 1 cut(s) 407
BspT107I GGYRCC 1 cut(s) 974
BsrDI GCAATG 1 cut(s) 933
BsrI ACTGG 1 cut(s) 978
BssECI CCNNGG 2 cut(s) 375, 448
BssMI GATC 2 cut(s) 622, 739
BssNI GRCGYC 1 cut(s) 975
BssSI CACGAG 2 cut(s) 316, 527
Bst2BI CACGAG 2 cut(s) 316, 527
Bst2UI CCWGG 1 cut(s) 842
Bst4CI ACNGT 4 cut(s) 23, 350, 502, 717
BstACI GRCGYC 1 cut(s) 975
BstAPI GCANNNNNTGC 2 cut(s) 583, 932
BstBI TTCGAA 1 cut(s) 407
BstC8I GCNNGC 5 cut(s) 223, 440, 553, 575, 665
BstDSI CCRYGG 2 cut(s) 375, 448
BstF5I GGATG 5 cut(s) 178, 286, 637, 749, 954
BstH2I RGCGCY 3 cut(s) 439, 910, 978
BstHHI GCGC 4 cut(s) 438, 634, 909, 977
BstKTI GATC 2 cut(s) 625, 742
BstMAI GTCTC 2 cut(s) 319, 866
BstMBI GATC 2 cut(s) 622, 739
BstMWI GCNNNNNNNGC 4 cut(s) 433, 583, 669, 932
BstNI CCWGG 1 cut(s) 842
BstNSI RCATGY 2 cut(s) 225, 879
BstSCI CCNGG 1 cut(s) 840
BstSFI CTRYAG 1 cut(s) 390
BstSLI GKGCMC 2 cut(s) 528, 1004
BstV1I GCAGC 3 cut(s) 646, 866, 1046
BstV2I GAAGAC 1 cut(s) 500
BsuRI GGCC 1 cut(s) 672
BtgI CCRYGG 2 cut(s) 375, 448
BtgZI GCGATG 1 cut(s) 601
BtsCI GGATG 5 cut(s) 178, 286, 637, 749, 954
BtsI GCAGTG 1 cut(s) 768
BtsIMutI CAGTG 2 cut(s) 507, 768
BveI ACCTGC 1 cut(s) 568
Cac8I GCNNGC 5 cut(s) 223, 440, 553, 575, 665
CciI TCATGA 1 cut(s) 625
CfoI GCGC 4 cut(s) 438, 634, 909, 977
Cfr13I GGNCC 2 cut(s) 4, 252
CseI GACGC 2 cut(s) 177, 1008
CsiI ACCWGGT 1 cut(s) 840
Csp6I GTAC 4 cut(s) 297, 601, 912, 954
CviAII CATG 8 cut(s) 222, 273, 311, 616, 626, 674, 860, 876
CviQI GTAC 4 cut(s) 297, 601, 912, 954
DinI GGCGCC 1 cut(s) 976
DpnI GATC 2 cut(s) 624, 741
DpnII GATC 2 cut(s) 622, 739
Eco47I GGWCC 2 cut(s) 4, 252
Eco47III AGCGCT 2 cut(s) 437, 908
Eco57I CTGAAG 2 cut(s) 149, 161
EcoRII CCWGG 1 cut(s) 840
EcoT22I ATGCAT 2 cut(s) 223, 274
EgeI GGCGCC 1 cut(s) 976
EheI GGCGCC 1 cut(s) 976
FaeI CATG 8 cut(s) 225, 276, 314, 619, 629, 677, 863, 879
FalI AAGNNNNNCTT 2 cut(s) 761, 793
FaqI GGGAC 1 cut(s) 357
FatI CATG 8 cut(s) 221, 272, 310, 615, 625, 673, 859, 875
FbaI TGATCA 1 cut(s) 622
Fnu4HI GCNGC 3 cut(s) 635, 880, 1060
FokI GGATG 5 cut(s) 185, 293, 624, 736, 961
Fsp4HI GCNGC 3 cut(s) 635, 880, 1060
FspBI CTAG 2 cut(s) 300, 891
GlaI GCGC 4 cut(s) 437, 633, 908, 976
GluI GCNGC 3 cut(s) 635, 880, 1060
HaeII RGCGCY 3 cut(s) 439, 910, 978
HaeIII GGCC 1 cut(s) 672
HapII CCGG 1 cut(s) 33
HgaI GACGC 2 cut(s) 177, 1008
HhaI GCGC 4 cut(s) 438, 634, 909, 977
Hin1I GRCGYC 1 cut(s) 975
Hin1II CATG 8 cut(s) 225, 276, 314, 619, 629, 677, 863, 879
Hin6I GCGC 4 cut(s) 436, 632, 907, 975
HinP1I GCGC 4 cut(s) 436, 632, 907, 975
HindIII AAGCTT 1 cut(s) 695
HinfI GANTC 3 cut(s) 364, 419, 798
HpaII CCGG 1 cut(s) 33
HphI GGTGA 3 cut(s) 113, 256, 502
Hpy166II GTNNAC 6 cut(s) 192, 379, 601, 954, 1002, 1023
Hpy188I TCNGA 4 cut(s) 12, 29, 141, 217
Hpy188III TCNNGA 6 cut(s) 167, 473, 509, 626, 869, 968
Hpy8I GTNNAC 6 cut(s) 192, 379, 601, 954, 1002, 1023
HpyAV CCTTC 4 cut(s) 245, 515, 766, 878
HpyCH4III ACNGT 4 cut(s) 23, 350, 502, 717
HpyF10VI GCNNNNNNNGC 4 cut(s) 433, 583, 669, 932
Hsp92I GRCGYC 1 cut(s) 975
Hsp92II CATG 8 cut(s) 225, 276, 314, 619, 629, 677, 863, 879
HspAI GCGC 4 cut(s) 436, 632, 907, 975
KasI GGCGCC 1 cut(s) 974
Ksp22I TGATCA 1 cut(s) 622
Kzo9I GATC 2 cut(s) 622, 739
LmnI GCTCC 2 cut(s) 237, 887
Lsp1109I GCAGC 3 cut(s) 646, 866, 1046
LweI GCATC 5 cut(s) 208, 259, 271, 514, 646
MabI ACCWGGT 1 cut(s) 840
MaeI CTAG 2 cut(s) 300, 891
MaeIII GTNAC 1 cut(s) 992
MalI GATC 2 cut(s) 624, 741
MboI GATC 2 cut(s) 622, 739
MboII GAAGA 4 cut(s) 62, 161, 505, 703
MhlI GDGCHC 2 cut(s) 528, 1004
MluCI AATT 3 cut(s) 127, 244, 512
Mly113I GGCGCC 1 cut(s) 975
MlyI GAGTC 1 cut(s) 792
MmeI TCCRAC 2 cut(s) 258, 513
Mph1103I ATGCAT 2 cut(s) 223, 274
MroXI GAANNNNTTC 1 cut(s) 653
MseI TTAA 3 cut(s) 93, 834, 939
MspA1I CMGCKG 1 cut(s) 1032
MspI CCGG 1 cut(s) 33
MspR9I CCNGG 1 cut(s) 842
Mva1269I GAATGC 1 cut(s) 575
MvaI CCWGG 1 cut(s) 842
MwoI GCNNNNNNNGC 4 cut(s) 433, 583, 669, 932
NarI GGCGCC 1 cut(s) 975
NdeII GATC 2 cut(s) 622, 739
NlaIII CATG 8 cut(s) 225, 276, 314, 619, 629, 677, 863, 879
NlaIV GGNNCC 2 cut(s) 113, 976
NmuCI GTSAC 1 cut(s) 992
NsiI ATGCAT 2 cut(s) 223, 274
NspI RCATGY 2 cut(s) 225, 879
NspV TTCGAA 1 cut(s) 407
PaeI GCATGC 1 cut(s) 225
PagI TCATGA 1 cut(s) 625
PctI GAATGC 1 cut(s) 575
PdmI GAANNNNTTC 1 cut(s) 653
PfeI GAWTC 2 cut(s) 364, 419
PflMI CCANNNNNTGG 1 cut(s) 984
PkrI GCNGC 3 cut(s) 636, 881, 1061
PleI GAGTC 1 cut(s) 792
PluTI GGCGCC 1 cut(s) 978
PpsI GAGTC 1 cut(s) 792
Psp6I CCWGG 1 cut(s) 840
PspGI CCWGG 1 cut(s) 840
PspN4I GGNNCC 2 cut(s) 113, 976
PspPI GGNCC 2 cut(s) 4, 252
PstI CTGCAG 1 cut(s) 394
PvuII CAGCTG 1 cut(s) 1032
RsaI GTAC 4 cut(s) 298, 602, 913, 955
RsaNI GTAC 4 cut(s) 297, 601, 912, 954
SaqAI TTAA 3 cut(s) 93, 834, 939
SatI GCNGC 3 cut(s) 635, 880, 1060
Sau3AI GATC 2 cut(s) 622, 739
Sau96I GGNCC 2 cut(s) 4, 252
ScaI AGTACT 1 cut(s) 298
SchI GAGTC 1 cut(s) 792
ScrFI CCNGG 1 cut(s) 842
SduI GDGCHC 2 cut(s) 528, 1004
SexAI ACCWGGT 1 cut(s) 840
SfaNI GCATC 5 cut(s) 208, 259, 271, 514, 646
SfcI CTRYAG 1 cut(s) 390
SfoI GGCGCC 1 cut(s) 976
SfuI TTCGAA 1 cut(s) 407
SinI GGWCC 2 cut(s) 4, 252
SmlI CTYRAG 1 cut(s) 698
SmoI CTYRAG 1 cut(s) 698
SpeI ACTAGT 1 cut(s) 299
SphI GCATGC 1 cut(s) 225
Sse9I AATT 3 cut(s) 127, 244, 512
SsiI CCGC 1 cut(s) 677
SspDI GGCGCC 1 cut(s) 974
SspMI CTAG 2 cut(s) 300, 891
StyD4I CCNGG 1 cut(s) 840
TaaI ACNGT 4 cut(s) 23, 350, 502, 717
TaqI TCGA 4 cut(s) 78, 242, 407, 657
TaqII GACCGA 1 cut(s) 269
TasI AATT 3 cut(s) 127, 244, 512
TatI WGTACW 3 cut(s) 296, 911, 953
TfiI GAWTC 2 cut(s) 364, 419
Tru1I TTAA 3 cut(s) 93, 834, 939
Tru9I TTAA 3 cut(s) 93, 834, 939
TscAI CASTG 2 cut(s) 507, 768
TseFI GTSAC 1 cut(s) 992
TseI GCWGC 3 cut(s) 634, 879, 1059
Tsp45I GTSAC 1 cut(s) 992
TspDTI ATGAA 2 cut(s) 63, 1035
TspGWI ACGGA 4 cut(s) 79, 412, 465, 661
TspRI CASTG 2 cut(s) 507, 768
Van91I CCANNNNNTGG 1 cut(s) 984
VneI GTGCAC 1 cut(s) 1000
VpaK11BI GGWCC 2 cut(s) 4, 252
XceI RCATGY 2 cut(s) 225, 879
XmnI GAANNNNTTC 1 cut(s) 653
XspI CTAG 2 cut(s) 300, 891
ZrmI AGTACT 1 cut(s) 298
Zsp2I ATGCAT 2 cut(s) 223, 274
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.