Rw3G008880

E3 ubiquitin-protein ligase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr3
OG0018171
Physical Location & Seq
Reverse (-)
8281621 .. 8283021
1401 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw3G008880.1

Sequence Viewer

Length: 1401 bp
ATGGACGATATGGACCATCAGAACGATGACAGTTTCAGAGCCGGAGAGCGTATAGATGAAGAAGCATATTATGATTGCCTTGAAGTCGATGACAAATCCGTTAAGTTGGGTGAGATTTGGTTCCCACAGAAAAAGAATTATACTATTCTGAAGCAAGAAGAAGTTGCCAAACTTCAGGAGGATGATATTGCACAAGCGTCCACTTTTCTTTCGGTATCAAAATCTGATGCATGCTTACTGCTCCTTCACTCGAATTGGAGGACCGATAATCTCACCGATGCATGGTATGCTGATGAAAATAAAATCAGAGAAAAAGCTGGCTTGTTGAAGAAACCCCTACTCGATAATGCCAGTAAAGATCCAAGCAGTAGTTGCCGACTATGTTCATATAGTAATAGGAGTAATATCTCAGCTTCTTGTGGTCATCCTTTTTGTGGAGATTGCTGGACAGGTTACATAACAAAATCAATCAATGAGGAGGGTCCTGCATGTTTGGTTTTGAGATGTCCTGAACCCGATTGTAGTGCAGTGGTTGGACCTGATTTAGTCCAAAACGTAATGTTGGCCACAAACCAAGAAGCTGAATGTTATGAGAGGTACAAGCATTACCTATTGAGATCTTATTTGGAAGATCACAAGTTGATCAAGTGGTGTCCTGCTCCGGATTGTAACTCCGCTATTAGGTTGGATGCTGGTGGTTCTAGTACTAGTTGCTATCATGTCTCGTGTGTTTGCTCTCGTAGTTATTGCTGGAACTGTGAGGAGGAGGAGAATCATCGTCCCGTGGACTGCCAAACTGCAGGGAAGTGGATTTCGAATATATTAGAATCCGTGCATTACCAGCGCTGGCAAAACCACGGAAAATCAAAGGAAAAAACTCTTGAAGTATTTGACGAGGTGAAGACGACAGTGCATGAAGAATTATTAGAAGGGCACGAGTTGGATTTTTTGTTAGAGGCAAGCAAACAGATGATTGAATGCAGGCAGGTATTGCGATGGATTGTTGTGTACGATTACTACAACATGAGTGATCATGAGGCGCAGCATCCTAAACGGAAGCTATTCGAGTGCTTGCAAGGCCATGCGGAGTTCAGCGTGAAGAAGCTTGAGAACCAAGTGCAACAGTTTCTCATCTTGGCTAATAGTGATCCCTCCTCATCCTCATCAGCAGTGGAAAACAAGGGCTTTGCAGACTTTCGTGTAAGACTCCAAGACCTTACAAATATGACAGCGACCTATTTTAAGAACCTGGTTAGGGCATTAGAGCATGGGCTTCCAGAGACATGCTGCGAAGGAGCTAGTTGGTCTTGTGAGCGCTGTACTTATATCAATGCAATGTCTGCCGTTAAATGTGGGATGTGTACTGCACACATTCCAGAGGGCCAGTTATTGGTCTTGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
IBR zf-RanBP

Protein Analysis

466

Amino Acids

53.1

Weight (kDa)

5.15

Isoelectric Point (pI)

55.7

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
UBA_ARI1 PF21235 58 - 96 8.2e-06 E3 ubiquitin-protein ligase ARIH1, UBA-like domain
IBR PF01485 198 - 264 1.1e-13 IBR domain, a half RING-finger domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0018171)

Species Orthologous Gene IDs
rosa_chinensis RchiOBHm_Chr3g0460891
rosa_laevigata RLG00000024969
rosa_multiflora Rmu_sc0008258.1_g000008 Rmu_sc0021377.1_g000001
rosa_roxburghii Rroxscaffold_6G00419050
rosa_rugosa Rorug03G0047000
rosa_samantha Rh3AG104300 Rh3BG108000 Rh3CG109700 Rh3DG109800
rosa_wichuraiana Rw3G008880

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 976
AccB7I CCANNNNNTGG 1 cut(s) 1390
AccIII TCCGGA 1 cut(s) 661
AciI CCGC 2 cut(s) 675, 1085
AclWI GGATC 2 cut(s) 353, 1142
AcoI YGGCCR 1 cut(s) 564
AcuI CTGAAG 2 cut(s) 158, 170
AfaI GTAC 5 cut(s) 599, 706, 1010, 1321, 1363
AfeI AGCGCT 2 cut(s) 845, 1316
AfiI CCNNNNNNNGG 5 cut(s) 282, 434, 681, 1255, 1390
AgsI TTSAA 4 cut(s) 83, 328, 884, 977
AhlI ACTAGT 1 cut(s) 707
AjnI CCWGG 1 cut(s) 1248
AluBI AGCT 6 cut(s) 317, 413, 581, 1060, 1105, 1298
AluI AGCT 6 cut(s) 317, 413, 581, 1060, 1105, 1298
Alw26I GTCTC 2 cut(s) 727, 1274
AlwI GGATC 2 cut(s) 353, 1142
Aor13HI TCCGGA 1 cut(s) 661
Aor51HI AGCGCT 2 cut(s) 845, 1316
AoxI GGCC 3 cut(s) 564, 1078, 1381
ApeKI GCWGC 2 cut(s) 1042, 1287
Asp700I GAANNNNTTC 1 cut(s) 1061
AspLEI GCGC 3 cut(s) 846, 1042, 1317
AspS9I GGNCC 5 cut(s) 13, 261, 482, 536, 1381
AsuHPI GGTGA 3 cut(s) 122, 265, 910
AsuII TTCGAA 1 cut(s) 815
AvaII GGWCC 4 cut(s) 13, 261, 482, 536
BaeGI GKGCMC 1 cut(s) 936
BalI TGGCCA 1 cut(s) 566
BauI CACGAG 2 cut(s) 724, 935
BbsI GAAGAC 1 cut(s) 908
BbvI GCAGC 2 cut(s) 1054, 1274
BccI CCATC 2 cut(s) 24, 990
BceAI ACGGC 1 cut(s) 1328
BcgI CGANNNNNNTGC 2 cut(s) 506, 540
BciT130I CCWGG 1 cut(s) 1250
BclI TGATCA 2 cut(s) 642, 1030
BcoDI GTCTC 2 cut(s) 727, 1274
BcuI ACTAGT 1 cut(s) 707
BfaI CTAG 3 cut(s) 702, 708, 1299
BfmI CTRYAG 1 cut(s) 798
BfoI RGCGCY 2 cut(s) 847, 1318
BfuAI ACCTGC 1 cut(s) 976
BglII AGATCT 1 cut(s) 617
BisI GCNGC 2 cut(s) 1043, 1288
BlsI GCNGC 2 cut(s) 1044, 1289
BmcAI AGTACT 1 cut(s) 706
Bme1390I CCNGG 1 cut(s) 1250
Bme18I GGWCC 4 cut(s) 13, 261, 482, 536
BmgT120I GGNCC 5 cut(s) 13, 261, 482, 536, 1381
BmiI GGNNCC 2 cut(s) 122, 483
BmrFI CCNGG 1 cut(s) 1250
BmsI GCATC 4 cut(s) 217, 268, 679, 1054
BpiI GAAGAC 1 cut(s) 908
Bpu14I TTCGAA 1 cut(s) 815
BpuEI CTTGAG 1 cut(s) 1127
BsaJI CCNNGG 2 cut(s) 783, 856
BsaWI WCCGGW 1 cut(s) 661
Bsc4I CCNNNNNNNGG 5 cut(s) 282, 434, 681, 1255, 1390
Bse1I ACTGG 2 cut(s) 351, 1384
Bse3DI GCAATG 1 cut(s) 1341
BseAI TCCGGA 1 cut(s) 661
BseBI CCWGG 1 cut(s) 1250
BseDI CCNNGG 2 cut(s) 783, 856
BseGI GGATG 6 cut(s) 187, 424, 694, 1045, 1157, 1362
BseLI CCNNNNNNNGG 5 cut(s) 282, 434, 681, 1255, 1390
BseMI GCAATG 1 cut(s) 1341
BseMII CTCAG 1 cut(s) 423
BseNI ACTGG 2 cut(s) 351, 1384
BseRI GAGGAG 5 cut(s) 491, 776, 779, 782, 1144
BseSI GKGCMC 1 cut(s) 936
BseXI GCAGC 2 cut(s) 1054, 1274
BsgI GTGCAG 2 cut(s) 546, 1350
BshFI GGCC 3 cut(s) 566, 1080, 1383
BsiSI CCGG 2 cut(s) 42, 662
BslFI GGGAC 1 cut(s) 765
BslI CCNNNNNNNGG 5 cut(s) 282, 434, 681, 1255, 1390
BsmAI GTCTC 2 cut(s) 727, 1274
BsmFI GGGAC 1 cut(s) 765
BsmI GAATGC 1 cut(s) 983
BsnI GGCC 3 cut(s) 566, 1080, 1383
Bsp119I TTCGAA 1 cut(s) 815
Bsp1286I GDGCHC 1 cut(s) 936
Bsp13I TCCGGA 1 cut(s) 661
Bsp143I GATC 6 cut(s) 358, 617, 631, 642, 1030, 1147
BspACI CCGC 2 cut(s) 675, 1085
BspANI GGCC 3 cut(s) 566, 1080, 1383
BspCNI CTCAG 1 cut(s) 422
BspEI TCCGGA 1 cut(s) 661
BspHI TCATGA 1 cut(s) 1033
BspLI GGNNCC 2 cut(s) 122, 483
BspMAI CTGCAG 1 cut(s) 802
BspMI ACCTGC 1 cut(s) 976
BspPI GGATC 2 cut(s) 353, 1142
BspT104I TTCGAA 1 cut(s) 815
BsrDI GCAATG 1 cut(s) 1341
BsrI ACTGG 2 cut(s) 351, 1384
BssECI CCNNGG 2 cut(s) 783, 856
BssMI GATC 6 cut(s) 358, 617, 631, 642, 1030, 1147
BssSI CACGAG 2 cut(s) 724, 935
Bst2BI CACGAG 2 cut(s) 724, 935
Bst2UI CCWGG 1 cut(s) 1250
Bst4CI ACNGT 4 cut(s) 32, 758, 910, 1125
BstAPI GCANNNNNTGC 4 cut(s) 287, 372, 991, 1340
BstBI TTCGAA 1 cut(s) 815
BstC8I GCNNGC 6 cut(s) 232, 319, 848, 961, 983, 1073
BstDEI CTNAG 1 cut(s) 409
BstDSI CCRYGG 2 cut(s) 783, 856
BstF5I GGATG 6 cut(s) 187, 424, 694, 1045, 1157, 1362
BstH2I RGCGCY 2 cut(s) 847, 1318
BstHHI GCGC 3 cut(s) 846, 1042, 1317
BstKTI GATC 6 cut(s) 361, 620, 634, 645, 1033, 1150
BstMAI GTCTC 2 cut(s) 727, 1274
BstMBI GATC 6 cut(s) 358, 617, 631, 642, 1030, 1147
BstMWI GCNNNNNNNGC 6 cut(s) 287, 372, 841, 991, 1077, 1340
BstNI CCWGG 1 cut(s) 1250
BstNSI RCATGY 3 cut(s) 234, 492, 1287
BstSCI CCNGG 1 cut(s) 1248
BstSFI CTRYAG 1 cut(s) 798
BstSLI GKGCMC 1 cut(s) 936
BstV1I GCAGC 2 cut(s) 1054, 1274
BstV2I GAAGAC 1 cut(s) 908
BstX2I RGATCY 2 cut(s) 358, 617
BstYI RGATCY 2 cut(s) 358, 617
BsuRI GGCC 3 cut(s) 566, 1080, 1383
BtgI CCRYGG 2 cut(s) 783, 856
BtgZI GCGATG 1 cut(s) 1009
BtsCI GGATG 6 cut(s) 187, 424, 694, 1045, 1157, 1362
BtsI GCAGTG 2 cut(s) 534, 1176
BtsIMutI CAGTG 3 cut(s) 534, 915, 1176
BveI ACCTGC 1 cut(s) 976
Cac8I GCNNGC 6 cut(s) 232, 319, 848, 961, 983, 1073
CciI TCATGA 1 cut(s) 1033
CfoI GCGC 3 cut(s) 846, 1042, 1317
Cfr13I GGNCC 5 cut(s) 13, 261, 482, 536, 1381
CseI GACGC 1 cut(s) 186
CsiI ACCWGGT 1 cut(s) 1248
Csp6I GTAC 5 cut(s) 598, 705, 1009, 1320, 1362
CviQI GTAC 5 cut(s) 598, 705, 1009, 1320, 1362
DdeI CTNAG 1 cut(s) 409
DpnI GATC 6 cut(s) 360, 619, 633, 644, 1032, 1149
DpnII GATC 6 cut(s) 358, 617, 631, 642, 1030, 1147
EaeI YGGCCR 1 cut(s) 564
Eco47I GGWCC 4 cut(s) 13, 261, 482, 536
Eco47III AGCGCT 2 cut(s) 845, 1316
Eco57I CTGAAG 2 cut(s) 158, 170
EcoO109I RGGNCCY 1 cut(s) 482
EcoRII CCWGG 1 cut(s) 1248
EcoT22I ATGCAT 2 cut(s) 232, 283
FaqI GGGAC 1 cut(s) 765
FbaI TGATCA 2 cut(s) 642, 1030
Fnu4HI GCNGC 2 cut(s) 1043, 1288
FokI GGATG 6 cut(s) 194, 411, 701, 1032, 1144, 1369
Fsp4HI GCNGC 2 cut(s) 1043, 1288
FspBI CTAG 3 cut(s) 702, 708, 1299
GlaI GCGC 3 cut(s) 845, 1041, 1316
GluI GCNGC 2 cut(s) 1043, 1288
HaeII RGCGCY 2 cut(s) 847, 1318
HaeIII GGCC 3 cut(s) 566, 1080, 1383
HapII CCGG 2 cut(s) 42, 662
HgaI GACGC 1 cut(s) 186
HhaI GCGC 3 cut(s) 846, 1042, 1317
Hin6I GCGC 3 cut(s) 844, 1040, 1315
HinP1I GCGC 3 cut(s) 844, 1040, 1315
HindIII AAGCTT 1 cut(s) 1103
HinfI GANTC 3 cut(s) 772, 827, 1206
HpaII CCGG 2 cut(s) 42, 662
HphI GGTGA 3 cut(s) 122, 265, 910
Hpy166II GTNNAC 4 cut(s) 201, 787, 1009, 1362
Hpy188I TCNGA 5 cut(s) 21, 38, 150, 226, 308
Hpy188III TCNNGA 7 cut(s) 176, 509, 662, 881, 1034, 1277, 1376
Hpy8I GTNNAC 4 cut(s) 201, 787, 1009, 1362
HpyAV CCTTC 3 cut(s) 254, 923, 1286
HpyCH4III ACNGT 4 cut(s) 32, 758, 910, 1125
HpyCH4IV ACGT 1 cut(s) 555
HpyF10VI GCNNNNNNNGC 6 cut(s) 287, 372, 841, 991, 1077, 1340
HpyF3I CTNAG 1 cut(s) 409
HpySE526I ACGT 1 cut(s) 555
HspAI GCGC 3 cut(s) 844, 1040, 1315
Kpn2I TCCGGA 1 cut(s) 661
Ksp22I TGATCA 2 cut(s) 642, 1030
Kzo9I GATC 6 cut(s) 358, 617, 631, 642, 1030, 1147
LmnI GCTCC 3 cut(s) 246, 664, 1295
Lsp1109I GCAGC 2 cut(s) 1054, 1274
LweI GCATC 4 cut(s) 217, 268, 679, 1054
MabI ACCWGGT 1 cut(s) 1248
MaeI CTAG 3 cut(s) 702, 708, 1299
MaeII ACGT 1 cut(s) 555
MaeIII GTNAC 2 cut(s) 452, 668
MalI GATC 6 cut(s) 360, 619, 633, 644, 1032, 1149
MboI GATC 6 cut(s) 358, 617, 631, 642, 1030, 1147
MboII GAAGA 7 cut(s) 71, 170, 340, 641, 913, 929, 1111
MflI RGATCY 2 cut(s) 358, 617
MhlI GDGCHC 1 cut(s) 936
MlsI TGGCCA 1 cut(s) 566
MluCI AATT 3 cut(s) 136, 253, 920
MluNI TGGCCA 1 cut(s) 566
MlyI GAGTC 1 cut(s) 1200
MmeI TCCRAC 3 cut(s) 514, 666, 921
Mox20I TGGCCA 1 cut(s) 566
Mph1103I ATGCAT 2 cut(s) 232, 283
MroI TCCGGA 1 cut(s) 661
MroXI GAANNNNTTC 1 cut(s) 1061
MscI TGGCCA 1 cut(s) 566
MseI TTAA 3 cut(s) 102, 1242, 1347
Msp20I TGGCCA 1 cut(s) 566
MspI CCGG 2 cut(s) 42, 662
MspR9I CCNGG 1 cut(s) 1250
Mva1269I GAATGC 1 cut(s) 983
MvaI CCWGG 1 cut(s) 1250
MwoI GCNNNNNNNGC 6 cut(s) 287, 372, 841, 991, 1077, 1340
NdeII GATC 6 cut(s) 358, 617, 631, 642, 1030, 1147
NlaIV GGNNCC 2 cut(s) 122, 483
NsiI ATGCAT 2 cut(s) 232, 283
NspI RCATGY 3 cut(s) 234, 492, 1287
NspV TTCGAA 1 cut(s) 815
PaeI GCATGC 1 cut(s) 234
PagI TCATGA 1 cut(s) 1033
PctI GAATGC 1 cut(s) 983
PdmI GAANNNNTTC 1 cut(s) 1061
PfeI GAWTC 2 cut(s) 772, 827
PflMI CCANNNNNTGG 1 cut(s) 1390
PkrI GCNGC 2 cut(s) 1044, 1289
PleI GAGTC 1 cut(s) 1200
PpsI GAGTC 1 cut(s) 1200
PpuMI RGGWCCY 1 cut(s) 482
Psp5II RGGWCCY 1 cut(s) 482
Psp6I CCWGG 1 cut(s) 1248
PspGI CCWGG 1 cut(s) 1248
PspN4I GGNNCC 2 cut(s) 122, 483
PspPI GGNCC 5 cut(s) 13, 261, 482, 536, 1381
PspPPI RGGWCCY 1 cut(s) 482
PstI CTGCAG 1 cut(s) 802
PsuI RGATCY 2 cut(s) 358, 617
RsaI GTAC 5 cut(s) 599, 706, 1010, 1321, 1363
RsaNI GTAC 5 cut(s) 598, 705, 1009, 1320, 1362
SaqAI TTAA 3 cut(s) 102, 1242, 1347
SatI GCNGC 2 cut(s) 1043, 1288
Sau3AI GATC 6 cut(s) 358, 617, 631, 642, 1030, 1147
Sau96I GGNCC 5 cut(s) 13, 261, 482, 536, 1381
ScaI AGTACT 1 cut(s) 706
SchI GAGTC 1 cut(s) 1200
ScrFI CCNGG 1 cut(s) 1250
SduI GDGCHC 1 cut(s) 936
SexAI ACCWGGT 1 cut(s) 1248
SfaNI GCATC 4 cut(s) 217, 268, 679, 1054
SfcI CTRYAG 1 cut(s) 798
SfuI TTCGAA 1 cut(s) 815
SinI GGWCC 4 cut(s) 13, 261, 482, 536
SmlI CTYRAG 1 cut(s) 1106
SmoI CTYRAG 1 cut(s) 1106
SpeI ACTAGT 1 cut(s) 707
SphI GCATGC 1 cut(s) 234
Sse9I AATT 3 cut(s) 136, 253, 920
SsiI CCGC 2 cut(s) 675, 1085
SspMI CTAG 3 cut(s) 702, 708, 1299
StyD4I CCNGG 1 cut(s) 1248
TaaI ACNGT 4 cut(s) 32, 758, 910, 1125
TaiI ACGT 1 cut(s) 558
TaqI TCGA 5 cut(s) 87, 251, 342, 815, 1065
TaqII GACCGA 1 cut(s) 278
TasI AATT 3 cut(s) 136, 253, 920
TatI WGTACW 3 cut(s) 704, 1319, 1361
TfiI GAWTC 2 cut(s) 772, 827
Tru1I TTAA 3 cut(s) 102, 1242, 1347
Tru9I TTAA 3 cut(s) 102, 1242, 1347
TscAI CASTG 3 cut(s) 534, 915, 1176
TseI GCWGC 2 cut(s) 1042, 1287
TspDTI ATGAA 4 cut(s) 72, 309, 375, 930
TspGWI ACGGA 4 cut(s) 88, 820, 873, 1069
TspRI CASTG 3 cut(s) 534, 915, 1176
Van91I CCANNNNNTGG 1 cut(s) 1390
VpaK11BI GGWCC 4 cut(s) 13, 261, 482, 536
XceI RCATGY 3 cut(s) 234, 492, 1287
XmnI GAANNNNTTC 1 cut(s) 1061
XspI CTAG 3 cut(s) 702, 708, 1299
ZrmI AGTACT 1 cut(s) 706
Zsp2I ATGCAT 2 cut(s) 232, 283
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.