RLG00000024969

E3 ubiquitin-protein ligase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr5
OG0018171
Physical Location & Seq
Forward (+)
40866652 .. 40867569
918 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000024969

Sequence Viewer

Length: 918 bp
ATGTTGGCCACAAACCAAGAAGCTGAATGTTATGAGAGGTACAAGCATTACCTACTGAGATCTTATTTGGAAGATCACAAGTTGATCAAGTGGTGTCCTGCTCCGGAATGTAACTCGGCTATTAGGTTGGATGCTGGTGGTTCTAGTACTAGTTGTTATCATGTCTCGTGTGTTTGCTCTCGTAGTTATTGCTGGAACTGTGAGGAGGAGGAGAATCATCGTCCCGTGGACTGCCACACTGCAGGGAAGTGGATTTCGAATATATTAGAATCCGTGCATTACCAGCGCTGGCAAAACCACGGAAAATCAAAGGAAAAAGCTCTTGAAGTATTTGACAAGGTGAAGACGACCGTGCATCAAGAATTATTGGAAGGGCACGAGTTGGGTTTTTTGTTAGAGGCATGCAAACAGATGATTGAATGCAGGCAGGTATTGCGATGGACTGTTGTATACGATTACTACAACATGAGTGATCATGAGGCGCAGCATCCTAAACGGAAGCTATTCAAGTGCTTGCAAGGCCATGCGGAGTTCAGCGTGAAGAAGCTTGAGAACCAAGTGCAACAGTTTCTCATCTTGGCTAATAGTGATCCCTCCTCATCCTCATCAGTAGTGGAAAAGAAGGACTTTGCAGACTTTCGTGTAAGACTCCAAGACCTTACAAATATGACAGCGACCTATTTTAAGAACCTGATTAGGGCATTAGAGCATGGGCTTCCAGAGACATGCTGCGAAGGAGCTAGTTGGTCTTGTGAGCTTTGTACTTATATCAATGCAATGTCTGCCGTTAAATGTGGGATGTGTACTGCACACATTCCAGAGGGCGCCGGTTATTGGTCTTGTGACAAGTGCACCTATTTCAATCCTGCGTCCACCACCACCAGCTGTTCGATGTGTTCATGGGCAGAGTGCTGCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
IBR zf-RanBP

Protein Analysis

306

Amino Acids

35.0

Weight (kDa)

6.16

Isoelectric Point (pI)

61.48

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
IBR PF01485 12 - 78 1.8e-13 IBR domain, a half RING-finger domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0018171)

Species Orthologous Gene IDs
rosa_chinensis RchiOBHm_Chr3g0460891
rosa_laevigata RLG00000024969
rosa_multiflora Rmu_sc0008258.1_g000008 Rmu_sc0021377.1_g000001
rosa_roxburghii Rroxscaffold_6G00419050
rosa_rugosa Rorug03G0047000
rosa_samantha Rh3AG104300 Rh3BG108000 Rh3CG109700 Rh3DG109800
rosa_wichuraiana Rw3G008880

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 418
AccB1I GGYRCC 1 cut(s) 824
AccI GTMKAC 1 cut(s) 450
AccIII TCCGGA 1 cut(s) 103
AciI CCGC 1 cut(s) 527
AclWI GGATC 1 cut(s) 584
AcoI YGGCCR 1 cut(s) 6
AcyI GRCGYC 1 cut(s) 825
AfaI GTAC 4 cut(s) 41, 148, 763, 805
AfeI AGCGCT 1 cut(s) 287
AfiI CCNNNNNNNGG 2 cut(s) 697, 834
AgsI TTSAA 4 cut(s) 326, 419, 508, 862
AhlI ACTAGT 1 cut(s) 149
AluBI AGCT 7 cut(s) 23, 320, 502, 547, 740, 757, 885
AluI AGCT 7 cut(s) 23, 320, 502, 547, 740, 757, 885
Alw21I GWGCWC 1 cut(s) 854
Alw26I GTCTC 2 cut(s) 169, 716
Alw44I GTGCAC 1 cut(s) 850
AlwI GGATC 1 cut(s) 584
Aor13HI TCCGGA 1 cut(s) 103
Aor51HI AGCGCT 1 cut(s) 287
AoxI GGCC 2 cut(s) 6, 520
ApaLI GTGCAC 1 cut(s) 850
ApeKI GCWGC 3 cut(s) 484, 729, 912
Asp700I GAANNNNTTC 1 cut(s) 503
AspLEI GCGC 3 cut(s) 288, 484, 827
AsuHPI GGTGA 1 cut(s) 352
AsuII TTCGAA 1 cut(s) 257
BaeGI GKGCMC 2 cut(s) 378, 854
BalI TGGCCA 1 cut(s) 8
BanI GGYRCC 1 cut(s) 824
BauI CACGAG 2 cut(s) 166, 377
BbsI GAAGAC 1 cut(s) 350
Bbv12I GWGCWC 1 cut(s) 854
BbvI GCAGC 3 cut(s) 496, 716, 899
BccI CCATC 1 cut(s) 432
BceAI ACGGC 1 cut(s) 770
BclI TGATCA 2 cut(s) 84, 472
BcoDI GTCTC 2 cut(s) 169, 716
BcuI ACTAGT 1 cut(s) 149
BfaI CTAG 3 cut(s) 144, 150, 741
BfmI CTRYAG 1 cut(s) 240
BfoI RGCGCY 2 cut(s) 289, 828
BfuAI ACCTGC 1 cut(s) 418
BglII AGATCT 1 cut(s) 59
BisI GCNGC 3 cut(s) 485, 730, 913
BlsI GCNGC 3 cut(s) 486, 731, 914
BmcAI AGTACT 1 cut(s) 148
BmiI GGNNCC 1 cut(s) 826
BmsI GCATC 3 cut(s) 121, 364, 496
BpiI GAAGAC 1 cut(s) 350
Bpu14I TTCGAA 1 cut(s) 257
BpuEI CTTGAG 1 cut(s) 569
BsaHI GRCGYC 1 cut(s) 825
BsaJI CCNNGG 2 cut(s) 225, 298
BsaWI WCCGGW 1 cut(s) 103
Bsc4I CCNNNNNNNGG 2 cut(s) 697, 834
Bse118I RCCGGY 1 cut(s) 827
Bse3DI GCAATG 1 cut(s) 783
BseAI TCCGGA 1 cut(s) 103
BseDI CCNNGG 2 cut(s) 225, 298
BseGI GGATG 4 cut(s) 136, 487, 599, 804
BseLI CCNNNNNNNGG 2 cut(s) 697, 834
BseMI GCAATG 1 cut(s) 783
BseMII CTCAG 1 cut(s) 47
BseRI GAGGAG 4 cut(s) 218, 221, 224, 586
BseSI GKGCMC 2 cut(s) 378, 854
BseXI GCAGC 3 cut(s) 496, 716, 899
BsgI GTGCAG 1 cut(s) 792
Bsh1285I CGRYCG 1 cut(s) 351
BshFI GGCC 2 cut(s) 8, 522
BshNI GGYRCC 1 cut(s) 824
BsiEI CGRYCG 1 cut(s) 351
BsiHKAI GWGCWC 1 cut(s) 854
BsiSI CCGG 2 cut(s) 104, 828
BslFI GGGAC 1 cut(s) 207
BslI CCNNNNNNNGG 2 cut(s) 697, 834
BsmAI GTCTC 2 cut(s) 169, 716
BsmFI GGGAC 1 cut(s) 207
BsmI GAATGC 1 cut(s) 425
BsnI GGCC 2 cut(s) 8, 522
Bsp119I TTCGAA 1 cut(s) 257
Bsp1286I GDGCHC 2 cut(s) 378, 854
Bsp13I TCCGGA 1 cut(s) 103
Bsp143I GATC 5 cut(s) 59, 73, 84, 472, 589
BspACI CCGC 1 cut(s) 527
BspANI GGCC 2 cut(s) 8, 522
BspCNI CTCAG 1 cut(s) 48
BspEI TCCGGA 1 cut(s) 103
BspHI TCATGA 1 cut(s) 475
BspLI GGNNCC 1 cut(s) 826
BspMAI CTGCAG 1 cut(s) 244
BspMI ACCTGC 1 cut(s) 418
BspPI GGATC 1 cut(s) 584
BspT104I TTCGAA 1 cut(s) 257
BspT107I GGYRCC 1 cut(s) 824
BsrDI GCAATG 1 cut(s) 783
BsrFI RCCGGY 1 cut(s) 827
BssAI RCCGGY 1 cut(s) 827
BssECI CCNNGG 2 cut(s) 225, 298
BssMI GATC 5 cut(s) 59, 73, 84, 472, 589
BssNAI GTATAC 1 cut(s) 451
BssNI GRCGYC 1 cut(s) 825
BssSI CACGAG 2 cut(s) 166, 377
Bst1107I GTATAC 1 cut(s) 451
Bst2BI CACGAG 2 cut(s) 166, 377
Bst4CI ACNGT 4 cut(s) 200, 352, 445, 567
BstACI GRCGYC 1 cut(s) 825
BstAPI GCANNNNNTGC 2 cut(s) 433, 782
BstBI TTCGAA 1 cut(s) 257
BstC8I GCNNGC 4 cut(s) 290, 403, 425, 515
BstDEI CTNAG 1 cut(s) 56
BstDSI CCRYGG 2 cut(s) 225, 298
BstF5I GGATG 4 cut(s) 136, 487, 599, 804
BstH2I RGCGCY 2 cut(s) 289, 828
BstHHI GCGC 3 cut(s) 288, 484, 827
BstKTI GATC 5 cut(s) 62, 76, 87, 475, 592
BstMAI GTCTC 2 cut(s) 169, 716
BstMBI GATC 5 cut(s) 59, 73, 84, 472, 589
BstMCI CGRYCG 1 cut(s) 351
BstMWI GCNNNNNNNGC 4 cut(s) 283, 433, 519, 782
BstNSI RCATGY 2 cut(s) 405, 729
BstSFI CTRYAG 1 cut(s) 240
BstSLI GKGCMC 2 cut(s) 378, 854
BstV1I GCAGC 3 cut(s) 496, 716, 899
BstV2I GAAGAC 1 cut(s) 350
BstX2I RGATCY 1 cut(s) 59
BstYI RGATCY 1 cut(s) 59
BstZ17I GTATAC 1 cut(s) 451
BsuRI GGCC 2 cut(s) 8, 522
BtgI CCRYGG 2 cut(s) 225, 298
BtgZI GCGATG 1 cut(s) 451
BtsCI GGATG 4 cut(s) 136, 487, 599, 804
BtsI GCAGTG 1 cut(s) 237
BtsIMutI CAGTG 1 cut(s) 237
BveI ACCTGC 1 cut(s) 418
Cac8I GCNNGC 4 cut(s) 290, 403, 425, 515
CciI TCATGA 1 cut(s) 475
CfoI GCGC 3 cut(s) 288, 484, 827
Cfr10I RCCGGY 1 cut(s) 827
CseI GACGC 1 cut(s) 858
Csp6I GTAC 4 cut(s) 40, 147, 762, 804
CviAII CATG 8 cut(s) 161, 402, 466, 476, 524, 710, 726, 900
CviQI GTAC 4 cut(s) 40, 147, 762, 804
DdeI CTNAG 1 cut(s) 56
DinI GGCGCC 1 cut(s) 826
DpnI GATC 5 cut(s) 61, 75, 86, 474, 591
DpnII GATC 5 cut(s) 59, 73, 84, 472, 589
EaeI YGGCCR 1 cut(s) 6
Eco47III AGCGCT 1 cut(s) 287
EgeI GGCGCC 1 cut(s) 826
EheI GGCGCC 1 cut(s) 826
FaeI CATG 8 cut(s) 164, 405, 469, 479, 527, 713, 729, 903
FalI AAGNNNNNCTT 2 cut(s) 611, 643
FaqI GGGAC 1 cut(s) 207
FatI CATG 8 cut(s) 160, 401, 465, 475, 523, 709, 725, 899
FbaI TGATCA 2 cut(s) 84, 472
FblI GTMKAC 1 cut(s) 450
Fnu4HI GCNGC 3 cut(s) 485, 730, 913
FokI GGATG 4 cut(s) 143, 474, 586, 811
Fsp4HI GCNGC 3 cut(s) 485, 730, 913
FspBI CTAG 3 cut(s) 144, 150, 741
GlaI GCGC 3 cut(s) 287, 483, 826
GluI GCNGC 3 cut(s) 485, 730, 913
HaeII RGCGCY 2 cut(s) 289, 828
HaeIII GGCC 2 cut(s) 8, 522
HapII CCGG 2 cut(s) 104, 828
HgaI GACGC 1 cut(s) 858
HhaI GCGC 3 cut(s) 288, 484, 827
Hin1I GRCGYC 1 cut(s) 825
Hin1II CATG 8 cut(s) 164, 405, 469, 479, 527, 713, 729, 903
Hin6I GCGC 3 cut(s) 286, 482, 825
HinP1I GCGC 3 cut(s) 286, 482, 825
HindIII AAGCTT 1 cut(s) 545
HinfI GANTC 3 cut(s) 214, 269, 648
HpaII CCGG 2 cut(s) 104, 828
HphI GGTGA 1 cut(s) 352
Hpy166II GTNNAC 5 cut(s) 229, 451, 804, 852, 873
Hpy188III TCNNGA 6 cut(s) 104, 323, 359, 476, 719, 818
Hpy8I GTNNAC 5 cut(s) 229, 451, 804, 852, 873
HpyAV CCTTC 3 cut(s) 365, 616, 728
HpyCH4III ACNGT 4 cut(s) 200, 352, 445, 567
HpyF10VI GCNNNNNNNGC 4 cut(s) 283, 433, 519, 782
HpyF3I CTNAG 1 cut(s) 56
Hsp92I GRCGYC 1 cut(s) 825
Hsp92II CATG 8 cut(s) 164, 405, 469, 479, 527, 713, 729, 903
HspAI GCGC 3 cut(s) 286, 482, 825
KasI GGCGCC 1 cut(s) 824
Kpn2I TCCGGA 1 cut(s) 103
Ksp22I TGATCA 2 cut(s) 84, 472
Kzo9I GATC 5 cut(s) 59, 73, 84, 472, 589
LmnI GCTCC 2 cut(s) 106, 737
Lsp1109I GCAGC 3 cut(s) 496, 716, 899
LweI GCATC 3 cut(s) 121, 364, 496
MaeI CTAG 3 cut(s) 144, 150, 741
MaeIII GTNAC 2 cut(s) 110, 842
MalI GATC 5 cut(s) 61, 75, 86, 474, 591
MboI GATC 5 cut(s) 59, 73, 84, 472, 589
MboII GAAGA 3 cut(s) 83, 355, 553
MflI RGATCY 1 cut(s) 59
MhlI GDGCHC 2 cut(s) 378, 854
MlsI TGGCCA 1 cut(s) 8
MluCI AATT 1 cut(s) 362
MluNI TGGCCA 1 cut(s) 8
Mly113I GGCGCC 1 cut(s) 825
MlyI GAGTC 1 cut(s) 642
MmeI TCCRAC 1 cut(s) 108
Mox20I TGGCCA 1 cut(s) 8
MroI TCCGGA 1 cut(s) 103
MroXI GAANNNNTTC 1 cut(s) 503
MscI TGGCCA 1 cut(s) 8
MseI TTAA 2 cut(s) 684, 789
Msp20I TGGCCA 1 cut(s) 8
MspA1I CMGCKG 1 cut(s) 885
MspI CCGG 2 cut(s) 104, 828
Mva1269I GAATGC 1 cut(s) 425
MwoI GCNNNNNNNGC 4 cut(s) 283, 433, 519, 782
NarI GGCGCC 1 cut(s) 825
NdeII GATC 5 cut(s) 59, 73, 84, 472, 589
NlaIII CATG 8 cut(s) 164, 405, 469, 479, 527, 713, 729, 903
NlaIV GGNNCC 1 cut(s) 826
NmeAIII GCCGAG 1 cut(s) 95
NmuCI GTSAC 1 cut(s) 842
NspI RCATGY 2 cut(s) 405, 729
NspV TTCGAA 1 cut(s) 257
PaeI GCATGC 1 cut(s) 405
PagI TCATGA 1 cut(s) 475
PctI GAATGC 1 cut(s) 425
PdmI GAANNNNTTC 1 cut(s) 503
PfeI GAWTC 2 cut(s) 214, 269
PkrI GCNGC 3 cut(s) 486, 731, 914
PleI GAGTC 1 cut(s) 642
PluTI GGCGCC 1 cut(s) 828
PpsI GAGTC 1 cut(s) 642
PspN4I GGNNCC 1 cut(s) 826
PstI CTGCAG 1 cut(s) 244
PsuI RGATCY 1 cut(s) 59
PvuII CAGCTG 1 cut(s) 885
RsaI GTAC 4 cut(s) 41, 148, 763, 805
RsaNI GTAC 4 cut(s) 40, 147, 762, 804
SaqAI TTAA 2 cut(s) 684, 789
SatI GCNGC 3 cut(s) 485, 730, 913
Sau3AI GATC 5 cut(s) 59, 73, 84, 472, 589
ScaI AGTACT 1 cut(s) 148
SchI GAGTC 1 cut(s) 642
SduI GDGCHC 2 cut(s) 378, 854
SfaNI GCATC 3 cut(s) 121, 364, 496
SfcI CTRYAG 1 cut(s) 240
SfoI GGCGCC 1 cut(s) 826
SfuI TTCGAA 1 cut(s) 257
SmlI CTYRAG 1 cut(s) 548
SmoI CTYRAG 1 cut(s) 548
SpeI ACTAGT 1 cut(s) 149
SphI GCATGC 1 cut(s) 405
Sse9I AATT 1 cut(s) 362
SsiI CCGC 1 cut(s) 527
SspDI GGCGCC 1 cut(s) 824
SspMI CTAG 3 cut(s) 144, 150, 741
TaaI ACNGT 4 cut(s) 200, 352, 445, 567
TaqI TCGA 2 cut(s) 257, 890
TasI AATT 1 cut(s) 362
TatI WGTACW 3 cut(s) 146, 761, 803
TfiI GAWTC 2 cut(s) 214, 269
Tru1I TTAA 2 cut(s) 684, 789
Tru9I TTAA 2 cut(s) 684, 789
TscAI CASTG 1 cut(s) 244
TseFI GTSAC 1 cut(s) 842
TseI GCWGC 3 cut(s) 484, 729, 912
Tsp45I GTSAC 1 cut(s) 842
TspDTI ATGAA 1 cut(s) 888
TspGWI ACGGA 3 cut(s) 262, 315, 511
TspRI CASTG 1 cut(s) 244
VneI GTGCAC 1 cut(s) 850
XceI RCATGY 2 cut(s) 405, 729
XmiI GTMKAC 1 cut(s) 450
XmnI GAANNNNTTC 1 cut(s) 503
XspI CTAG 3 cut(s) 144, 150, 741
ZrmI AGTACT 1 cut(s) 148
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.