RchiOBHm_Chr3g0474671

GMC oxidoreductase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
3
OG0018139
Physical Location & Seq
Reverse (-)
20394322 .. 20395320
999 bp
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UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 759 bp
ATGGAGGAGCAAGCAGAGGTAGAAAGCAGTTTTGGACATGAAAGTTATGATGCAGTTGTTGTGGGGTCTGGTTATGGCGGTTCTGTTGCTGCTTGTCGGCTGGCTATGGCAGGCGTAAGGGTGTGTCTACTTGAAAAAGGCCGAAAATGGGAAGCGCAGGACTTCCCAACTGACAGCTTCAAAATCTTATCGGCTGTGAGGATGGAGAACCAGAACCTAGGTGTTAGCTATGGACCAAAAGACGCTTTATTTCAGGTGTACGAGCAAAATGATTCTCTAGCAGCAGTGGCTTGTGGGCTTGGTGGAGGTTCACTAGTGAATGCTGGAGTTATGATGCCAGCACCTGTTCGTGCTAGACGGCATCCAAAATGGCCGAAGGAATGGGAAAGGAATTGGGGTAGTTGTGAAGCTTCTGCTGCAGACATGCTGAGAATACAAAGCATTCCCGTCAAGTTTCCTTCCGCGAAAATTTTGGAAGAAGCTGCTGATGGAGTGACTGTTGACACTTCAATAAAGCTTAGTGTGAATTTTGACATTGAAGAACCAACAACAAATGGAACGAAGCGTCCACAGATGGGTAGCTGCTTAGCCTGTGGAAACTGTCTTTCTGGATGTCCATATAACGCCAAAAGTTCCACTGACAAAAATTATCTTGTCTCAGCAATCCAGGCATGTCTCAAGTCTTTTTACTTGTTGTCTAATATATCCATAGTTATTGTATGTCAAAATTTCTTCCGCCATTATCAGTCCAGAGGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
GMC_oxred_C GMC_oxred_N

Protein Analysis

252

Amino Acids

27.34

Weight (kDa)

6.32

Isoelectric Point (pI)

51.17

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GMC_oxred_N PF00732 17 - 226 2.2e-07 GMC oxidoreductase
NAD_binding_8 PF13450 20 - 48 7.3e-06 NAD(P)-binding Rossmann-like domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0018139)

Species Orthologous Gene IDs
pyrus_communis pycom10g15180 pycom10g15190
rosa_chinensis RchiOBHm_Chr3g0474671
rosa_multiflora Rmu_sc0001353.1_g000047 Rmu_sc0002324.1_g000028
rosa_roxburghii Rroxscaffold_6G00407000
rosa_rugosa Rorug03G0142000
rosa_samantha Rh3AG192800 Rh3BG222700 Rh3CG217400 Rh3DG218300

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 127
AccII CGCG 1 cut(s) 464
AciI CCGC 3 cut(s) 78, 462, 736
AcoI YGGCCR 1 cut(s) 371
AcsI RAATTY 3 cut(s) 468, 526, 727
AfaI GTAC 1 cut(s) 260
AfiI CCNNNNNNNGG 2 cut(s) 148, 575
AgsI TTSAA 4 cut(s) 134, 181, 510, 539
AhlI ACTAGT 1 cut(s) 313
AjnI CCWGG 1 cut(s) 666
AjuI GAANNNNNNNTTGG 2 cut(s) 15, 47
AloI GAACNNNNNNTCC 2 cut(s) 550, 582
AluBI AGCT 6 cut(s) 177, 228, 410, 482, 517, 582
AluI AGCT 6 cut(s) 177, 228, 410, 482, 517, 582
Alw26I GTCTC 2 cut(s) 661, 680
AlwNI CAGNNNCTG 1 cut(s) 344
AoxI GGCC 2 cut(s) 139, 371
ApeKI GCWGC 5 cut(s) 89, 281, 416, 482, 582
ApoI RAATTY 3 cut(s) 468, 526, 727
AspA2I CCTAGG 1 cut(s) 217
AspLEI GCGC 1 cut(s) 157
AspS9I GGNCC 1 cut(s) 233
AvaII GGWCC 1 cut(s) 233
AvrII CCTAGG 1 cut(s) 217
BbvI GCAGC 5 cut(s) 76, 293, 403, 469, 569
BccI CCATC 3 cut(s) 196, 482, 568
BceAI ACGGC 1 cut(s) 374
BciT130I CCWGG 1 cut(s) 668
BcoDI GTCTC 2 cut(s) 661, 680
BcuI ACTAGT 1 cut(s) 313
BfaI CTAG 4 cut(s) 218, 278, 314, 354
BfmI CTRYAG 1 cut(s) 417
BisI GCNGC 5 cut(s) 90, 282, 417, 483, 583
BlnI CCTAGG 1 cut(s) 217
BlpI GCTNAGC 1 cut(s) 586
BlsI GCNGC 5 cut(s) 91, 283, 418, 484, 584
Bme1390I CCNGG 1 cut(s) 668
Bme18I GGWCC 1 cut(s) 233
BmgT120I GGNCC 1 cut(s) 233
BmrFI CCNGG 1 cut(s) 668
BmsI GCATC 3 cut(s) 40, 324, 370
BpmI CTGGAG 1 cut(s) 345
Bpu1102I GCTNAGC 1 cut(s) 586
BpuEI CTTGAG 1 cut(s) 662
BsaJI CCNNGG 1 cut(s) 217
BsaXI ACNNNNNCTCC 2 cut(s) 483, 513
Bsc4I CCNNNNNNNGG 2 cut(s) 148, 575
BseBI CCWGG 1 cut(s) 668
BseDI CCNNGG 1 cut(s) 217
BseGI GGATG 3 cut(s) 207, 361, 617
BseLI CCNNNNNNNGG 2 cut(s) 148, 575
BseMII CTCAG 2 cut(s) 419, 672
BseRI GAGGAG 1 cut(s) 20
BseXI GCAGC 5 cut(s) 76, 293, 403, 469, 569
Bsh1236I CGCG 1 cut(s) 464
BshFI GGCC 2 cut(s) 141, 373
BslI CCNNNNNNNGG 2 cut(s) 148, 575
BsmAI GTCTC 2 cut(s) 661, 680
BsmI GAATGC 2 cut(s) 325, 441
BsnI GGCC 2 cut(s) 141, 373
Bsp1720I GCTNAGC 1 cut(s) 586
BspACI CCGC 3 cut(s) 78, 462, 736
BspANI GGCC 2 cut(s) 141, 373
BspCNI CTCAG 2 cut(s) 420, 671
BspFNI CGCG 1 cut(s) 464
BspMAI CTGCAG 1 cut(s) 421
BssECI CCNNGG 1 cut(s) 217
BssT1I CCWWGG 1 cut(s) 217
Bst2UI CCWGG 1 cut(s) 668
Bst4CI ACNGT 2 cut(s) 499, 602
BstC8I GCNNGC 4 cut(s) 12, 102, 112, 339
BstDEI CTNAG 4 cut(s) 428, 518, 586, 658
BstF5I GGATG 3 cut(s) 207, 361, 617
BstFNI CGCG 1 cut(s) 464
BstHHI GCGC 1 cut(s) 157
BstMAI GTCTC 2 cut(s) 661, 680
BstMWI GCNNNNNNNGC 3 cut(s) 287, 416, 668
BstNI CCWGG 1 cut(s) 668
BstNSI RCATGY 2 cut(s) 427, 675
BstSCI CCNGG 1 cut(s) 666
BstSFI CTRYAG 1 cut(s) 417
BstUI CGCG 1 cut(s) 464
BstV1I GCAGC 5 cut(s) 76, 293, 403, 469, 569
BsuRI GGCC 2 cut(s) 141, 373
BtsCI GGATG 3 cut(s) 207, 361, 617
BtsI GCAGTG 1 cut(s) 291
BtsIMutI CAGTG 2 cut(s) 291, 636
Cac8I GCNNGC 4 cut(s) 12, 102, 112, 339
CaiI CAGNNNCTG 1 cut(s) 344
CfoI GCGC 1 cut(s) 157
Cfr13I GGNCC 1 cut(s) 233
CseI GACGC 2 cut(s) 251, 554
Csp6I GTAC 1 cut(s) 259
CviAII CATG 3 cut(s) 38, 424, 672
CviQI GTAC 1 cut(s) 259
DdeI CTNAG 4 cut(s) 428, 518, 586, 658
EaeI YGGCCR 1 cut(s) 371
EciI GGCGGA 1 cut(s) 725
Eco130I CCWWGG 1 cut(s) 217
Eco47I GGWCC 1 cut(s) 233
EcoRII CCWGG 1 cut(s) 666
EcoT14I CCWWGG 1 cut(s) 217
ErhI CCWWGG 1 cut(s) 217
FaeI CATG 3 cut(s) 41, 427, 675
FatI CATG 3 cut(s) 37, 423, 671
FblI GTMKAC 1 cut(s) 127
Fnu4HI GCNGC 5 cut(s) 90, 282, 417, 483, 583
FokI GGATG 3 cut(s) 214, 348, 624
Fsp4HI GCNGC 5 cut(s) 90, 282, 417, 483, 583
FspBI CTAG 4 cut(s) 218, 278, 314, 354
GlaI GCGC 1 cut(s) 156
GluI GCNGC 5 cut(s) 90, 282, 417, 483, 583
GsuI CTGGAG 1 cut(s) 345
HaeIII GGCC 2 cut(s) 141, 373
HgaI GACGC 2 cut(s) 251, 554
HhaI GCGC 1 cut(s) 157
Hin1II CATG 3 cut(s) 41, 427, 675
Hin6I GCGC 1 cut(s) 155
HinP1I GCGC 1 cut(s) 155
HincII GTYRAC 1 cut(s) 502
HindII GTYRAC 1 cut(s) 502
HindIII AAGCTT 2 cut(s) 408, 515
HinfI GANTC 1 cut(s) 272
Hpy166II GTNNAC 5 cut(s) 128, 259, 311, 502, 569
Hpy188III TCNNGA 2 cut(s) 609, 750
Hpy8I GTNNAC 5 cut(s) 128, 259, 311, 502, 569
HpyAV CCTTC 2 cut(s) 370, 468
HpyCH4III ACNGT 2 cut(s) 499, 602
HpyCH4V TGCA 2 cut(s) 53, 419
HpyF10VI GCNNNNNNNGC 3 cut(s) 287, 416, 668
HpyF3I CTNAG 4 cut(s) 428, 518, 586, 658
Hsp92II CATG 3 cut(s) 41, 427, 675
HspAI GCGC 1 cut(s) 155
LmnI GCTCC 1 cut(s) 7
Lsp1109I GCAGC 5 cut(s) 76, 293, 403, 469, 569
LweI GCATC 3 cut(s) 40, 324, 370
MaeI CTAG 4 cut(s) 218, 278, 314, 354
MaeIII GTNAC 1 cut(s) 493
MboII GAAGA 3 cut(s) 488, 551, 724
MluCI AATT 5 cut(s) 391, 468, 526, 646, 727
MnlI CCTC 4 cut(s) 10, 192, 299, 746
MspR9I CCNGG 1 cut(s) 668
Mva1269I GAATGC 2 cut(s) 325, 441
MvaI CCWGG 1 cut(s) 668
MvnI CGCG 1 cut(s) 464
MwoI GCNNNNNNNGC 3 cut(s) 287, 416, 668
NlaIII CATG 3 cut(s) 41, 427, 675
NmuCI GTSAC 1 cut(s) 493
NspI RCATGY 2 cut(s) 427, 675
PctI GAATGC 2 cut(s) 325, 441
PfeI GAWTC 1 cut(s) 272
PkrI GCNGC 5 cut(s) 91, 283, 418, 484, 584
Psp6I CCWGG 1 cut(s) 666
PspGI CCWGG 1 cut(s) 666
PspPI GGNCC 1 cut(s) 233
PstI CTGCAG 1 cut(s) 421
PstNI CAGNNNCTG 1 cut(s) 344
RsaI GTAC 1 cut(s) 260
RsaNI GTAC 1 cut(s) 259
SatI GCNGC 5 cut(s) 90, 282, 417, 483, 583
Sau96I GGNCC 1 cut(s) 233
ScrFI CCNGG 1 cut(s) 668
SfaNI GCATC 3 cut(s) 40, 324, 370
SfcI CTRYAG 1 cut(s) 417
SinI GGWCC 1 cut(s) 233
SmlI CTYRAG 1 cut(s) 677
SmoI CTYRAG 1 cut(s) 677
SpeI ACTAGT 1 cut(s) 313
Sse9I AATT 5 cut(s) 391, 468, 526, 646, 727
SsiI CCGC 3 cut(s) 78, 462, 736
SspMI CTAG 4 cut(s) 218, 278, 314, 354
StyD4I CCNGG 1 cut(s) 666
StyI CCWWGG 1 cut(s) 217
TaaI ACNGT 2 cut(s) 499, 602
TasI AATT 5 cut(s) 391, 468, 526, 646, 727
TfiI GAWTC 1 cut(s) 272
TscAI CASTG 2 cut(s) 291, 643
TseFI GTSAC 1 cut(s) 493
TseI GCWGC 5 cut(s) 89, 281, 416, 482, 582
Tsp45I GTSAC 1 cut(s) 493
TspDTI ATGAA 1 cut(s) 54
TspRI CASTG 2 cut(s) 291, 643
VpaK11BI GGWCC 1 cut(s) 233
XapI RAATTY 3 cut(s) 468, 526, 727
XceI RCATGY 2 cut(s) 427, 675
XmaJI CCTAGG 1 cut(s) 217
XmiI GTMKAC 1 cut(s) 127
XspI CTAG 4 cut(s) 218, 278, 314, 354
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.