RchiOBHm_Chr3g0488501

Small ubiquitinrelated modifier

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
3
OG0017974
Physical Location & Seq
Forward (+)
36149459 .. 36150562
1104 bp
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UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 312 bp
ATGTCCGGCATCATGAACAGAAACAAAGACAATGGCAAGAAGCCAGCCGCCGCCTCCGGGCAGAACTCCACCGACGTCAACCTCAAAGTCAAGAACCAGAAATTGAAAACAATGTACTTCAGGATGAGGCGCAATACTCCGCTCCAGGAGCTTATTGATGTTTACTGTAAGAAAAACGACGTTTACCACTTCCGATTCCTCTTTGATGGCCATGGGATTAATCCCAAGCTCACTGCACTCCAGTCTGGAATGAAAGATGGGGATGAAATTTCTGCAATGTTGCACGTTGACGGAGGTGGTCGACGTGGCTAG

Protein Analysis

103

Amino Acids

11.59

Weight (kDa)

9.88

Isoelectric Point (pI)

35.89

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Rad60-SLD PF11976 27 - 94 5.6e-15 Ubiquitin-2 like Rad60 SUMO-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0017974)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g27530
rosa_chinensis RchiOBHm_Chr3g0488501
rosa_multiflora Rmu_sc0005363.1_g000003
rosa_roxburghii Rroxscaffold_6G00394210
rosa_rugosa Rorug03G0238600 Rorug03G0238600
rosa_samantha Rh3AG287500 Rh3BG324400 Rh3CG321700 Rh3DG320400
rosa_wichuraiana Rw3G025460

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 78
AccBSI CCGCTC 1 cut(s) 142
AccI GTMKAC 1 cut(s) 301
AciI CCGC 3 cut(s) 48, 51, 140
AcoI YGGCCR 1 cut(s) 208
AcsI RAATTY 1 cut(s) 267
AcuI CTGAAG 1 cut(s) 103
AcyI GRCGYC 1 cut(s) 75
AfaI GTAC 1 cut(s) 116
AfiI CCNNNNNNNGG 1 cut(s) 57
AgsI TTSAA 1 cut(s) 106
AjiI CACGTC 1 cut(s) 305
AjnI CCWGG 1 cut(s) 144
AluBI AGCT 2 cut(s) 151, 229
AluI AGCT 2 cut(s) 151, 229
AoxI GGCC 1 cut(s) 208
ApoI RAATTY 1 cut(s) 267
AseI ATTAAT 1 cut(s) 219
AspLEI GCGC 1 cut(s) 132
AsuC2I CCSGG 1 cut(s) 58
BalI TGGCCA 1 cut(s) 210
BccI CCATC 2 cut(s) 200, 251
BciT130I CCWGG 1 cut(s) 146
BcnI CCSGG 1 cut(s) 58
BfaI CTAG 1 cut(s) 310
BisI GCNGC 2 cut(s) 48, 51
BlsI GCNGC 2 cut(s) 49, 52
Bme1390I CCNGG 2 cut(s) 58, 146
BmgBI CACGTC 1 cut(s) 305
BmrFI CCNGG 2 cut(s) 58, 146
BmsI GCATC 1 cut(s) 18
BpmI CTGGAG 2 cut(s) 128, 224
BpuMI CCSGG 1 cut(s) 58
BsaHI GRCGYC 1 cut(s) 75
BsaJI CCNNGG 1 cut(s) 211
Bsc4I CCNNNNNNNGG 1 cut(s) 57
Bse1I ACTGG 1 cut(s) 241
Bse3DI GCAATG 1 cut(s) 282
BseBI CCWGG 1 cut(s) 146
BseDI CCNNGG 1 cut(s) 211
BseGI GGATG 2 cut(s) 129, 268
BseLI CCNNNNNNNGG 1 cut(s) 57
BseMI GCAATG 1 cut(s) 282
BseNI ACTGG 1 cut(s) 241
BsgI GTGCAG 1 cut(s) 219
BshFI GGCC 1 cut(s) 210
BsiSI CCGG 2 cut(s) 6, 57
BslI CCNNNNNNNGG 1 cut(s) 57
BsnI GGCC 1 cut(s) 210
Bsp19I CCATGG 1 cut(s) 211
BspACI CCGC 3 cut(s) 48, 51, 140
BspANI GGCC 1 cut(s) 210
BspHI TCATGA 1 cut(s) 12
BsrBI CCGCTC 1 cut(s) 142
BsrDI GCAATG 1 cut(s) 282
BsrI ACTGG 1 cut(s) 241
BssECI CCNNGG 1 cut(s) 211
BssNI GRCGYC 1 cut(s) 75
BssT1I CCWWGG 1 cut(s) 211
Bst2UI CCWGG 1 cut(s) 146
Bst4CI ACNGT 1 cut(s) 167
BstACI GRCGYC 1 cut(s) 75
BstC8I GCNNGC 1 cut(s) 45
BstDSI CCRYGG 1 cut(s) 211
BstF5I GGATG 2 cut(s) 129, 268
BstHHI GCGC 1 cut(s) 132
BstMWI GCNNNNNNNGC 1 cut(s) 148
BstNI CCWGG 1 cut(s) 146
BstSCI CCNGG 2 cut(s) 56, 144
BsuRI GGCC 1 cut(s) 210
BtgI CCRYGG 1 cut(s) 211
BtrI CACGTC 1 cut(s) 305
BtsCI GGATG 2 cut(s) 129, 268
BtsI GCAGTG 1 cut(s) 231
BtsIMutI CAGTG 1 cut(s) 231
Cac8I GCNNGC 1 cut(s) 45
CciI TCATGA 1 cut(s) 12
CfoI GCGC 1 cut(s) 132
Csp6I GTAC 1 cut(s) 115
CviAII CATG 2 cut(s) 13, 212
CviJI RGCY 6 cut(s) 43, 47, 151, 210, 229, 309
CviKI_1 RGCY 6 cut(s) 43, 47, 151, 210, 229, 309
CviQI GTAC 1 cut(s) 115
EaeI YGGCCR 1 cut(s) 208
Eco130I CCWWGG 1 cut(s) 211
Eco57I CTGAAG 1 cut(s) 103
EcoRII CCWGG 1 cut(s) 144
EcoT14I CCWWGG 1 cut(s) 211
ErhI CCWWGG 1 cut(s) 211
FaeI CATG 2 cut(s) 16, 215
FaiI YATR 2 cut(s) 14, 213
FatI CATG 2 cut(s) 12, 211
FblI GTMKAC 1 cut(s) 301
Fnu4HI GCNGC 2 cut(s) 48, 51
FokI GGATG 2 cut(s) 136, 275
Fsp4HI GCNGC 2 cut(s) 48, 51
FspBI CTAG 1 cut(s) 310
GlaI GCGC 1 cut(s) 131
GluI GCNGC 2 cut(s) 48, 51
GsuI CTGGAG 2 cut(s) 128, 224
HaeIII GGCC 1 cut(s) 210
HapII CCGG 2 cut(s) 6, 57
HhaI GCGC 1 cut(s) 132
Hin1I GRCGYC 1 cut(s) 75
Hin1II CATG 2 cut(s) 16, 215
Hin6I GCGC 1 cut(s) 130
HinP1I GCGC 1 cut(s) 130
HincII GTYRAC 3 cut(s) 79, 289, 302
HindII GTYRAC 3 cut(s) 79, 289, 302
HinfI GANTC 1 cut(s) 195
HpaII CCGG 2 cut(s) 6, 57
Hpy166II GTNNAC 5 cut(s) 79, 163, 184, 289, 302
Hpy188I TCNGA 1 cut(s) 194
Hpy188III TCNNGA 4 cut(s) 13, 91, 121, 246
Hpy8I GTNNAC 5 cut(s) 79, 163, 184, 289, 302
Hpy99I CGWCG 3 cut(s) 77, 182, 306
HpyCH4III ACNGT 1 cut(s) 167
HpyCH4IV ACGT 4 cut(s) 75, 180, 285, 304
HpyCH4V TGCA 3 cut(s) 236, 275, 283
HpyF10VI GCNNNNNNNGC 1 cut(s) 148
HpySE526I ACGT 4 cut(s) 75, 180, 285, 304
Hsp92I GRCGYC 1 cut(s) 75
Hsp92II CATG 2 cut(s) 16, 215
HspAI GCGC 1 cut(s) 130
LmnI GCTCC 2 cut(s) 147, 148
LpnPI CCDG 9 cut(s) 19, 57, 70, 106, 110, 131, 158, 231, 254
LweI GCATC 1 cut(s) 18
MaeI CTAG 1 cut(s) 310
MaeII ACGT 4 cut(s) 75, 180, 285, 304
MbiI CCGCTC 1 cut(s) 142
MlsI TGGCCA 1 cut(s) 210
MluCI AATT 2 cut(s) 101, 267
MluNI TGGCCA 1 cut(s) 210
MnlI CCTC 5 cut(s) 64, 92, 120, 209, 287
Mox20I TGGCCA 1 cut(s) 210
MscI TGGCCA 1 cut(s) 210
MseI TTAA 1 cut(s) 219
Msp20I TGGCCA 1 cut(s) 210
MspI CCGG 2 cut(s) 6, 57
MspR9I CCNGG 2 cut(s) 58, 146
MvaI CCWGG 1 cut(s) 146
MwoI GCNNNNNNNGC 1 cut(s) 148
NciI CCSGG 1 cut(s) 58
NcoI CCATGG 1 cut(s) 211
NlaIII CATG 2 cut(s) 16, 215
PagI TCATGA 1 cut(s) 12
PfeI GAWTC 1 cut(s) 195
PfoI TCCNGGA 1 cut(s) 144
PkrI GCNGC 2 cut(s) 49, 52
PshBI ATTAAT 1 cut(s) 219
Psp6I CCWGG 1 cut(s) 144
PspGI CCWGG 1 cut(s) 144
RsaI GTAC 1 cut(s) 116
RsaNI GTAC 1 cut(s) 115
SalI GTCGAC 1 cut(s) 300
SaqAI TTAA 1 cut(s) 219
SatI GCNGC 2 cut(s) 48, 51
ScrFI CCNGG 2 cut(s) 58, 146
SetI ASST 8 cut(s) 78, 84, 153, 183, 231, 288, 298, 307
SfaNI GCATC 1 cut(s) 18
Sse9I AATT 2 cut(s) 101, 267
SsiI CCGC 3 cut(s) 48, 51, 140
SspMI CTAG 1 cut(s) 310
StyD4I CCNGG 2 cut(s) 56, 144
StyI CCWWGG 1 cut(s) 211
TaaI ACNGT 1 cut(s) 167
TaiI ACGT 4 cut(s) 78, 183, 288, 307
TaqI TCGA 1 cut(s) 301
TasI AATT 2 cut(s) 101, 267
TatI WGTACW 1 cut(s) 114
TauI GCSGC 2 cut(s) 50, 53
TfiI GAWTC 1 cut(s) 195
Tru1I TTAA 1 cut(s) 219
Tru9I TTAA 1 cut(s) 219
TscAI CASTG 1 cut(s) 238
TspDTI ATGAA 3 cut(s) 29, 266, 279
TspGWI ACGGA 1 cut(s) 306
TspRI CASTG 1 cut(s) 238
VspI ATTAAT 1 cut(s) 219
XapI RAATTY 1 cut(s) 267
XmiI GTMKAC 1 cut(s) 301
XspI CTAG 1 cut(s) 310
ZraI GACGTC 1 cut(s) 76
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.