Rh3BG324400

Small ubiquitinrelated modifier

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr3B
OG0017974
Physical Location & Seq
Forward (+)
33697971 .. 33698948
978 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh3BG324400.1

Sequence Viewer

Length: 300 bp
ATGAACACAAACAAAGACAATGGCAAGAAGCCAACCGCCGCCTCCGGGCAGAACTCCACCGACGTCAACCTCAAAGTCAAGAACCAGAAATTGAAAACAATGTACTTCAGGATGAGGCGCAATACTCCGCTCCAGGAGCTTATTGATGTTTACTGTAAGAAAAACGACGTGTACCACTTCCGATTCCTCTTTGATGGCCATGGGATTAATCCCAAGCTCACTGCACTCCAGTCTGGAATGAAAGATGGGGATGAAATTTCTGCAATGTTGCACGTTGACGGAGGTGGTCGACGTGGCTAG

Protein Analysis

99

Amino Acids

11.17

Weight (kDa)

9.76

Isoelectric Point (pI)

27.79

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Rad60-SLD PF11976 23 - 90 5e-15 Ubiquitin-2 like Rad60 SUMO-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0017974)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g27530
rosa_chinensis RchiOBHm_Chr3g0488501
rosa_multiflora Rmu_sc0005363.1_g000003
rosa_roxburghii Rroxscaffold_6G00394210
rosa_rugosa Rorug03G0238600 Rorug03G0238600
rosa_samantha Rh3AG287500 Rh3BG324400 Rh3CG321700 Rh3DG320400
rosa_wichuraiana Rw3G025460

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 66
AccBSI CCGCTC 1 cut(s) 130
AccI GTMKAC 1 cut(s) 289
AciI CCGC 3 cut(s) 36, 39, 128
AcoI YGGCCR 1 cut(s) 196
AcsI RAATTY 1 cut(s) 255
AcuI CTGAAG 1 cut(s) 91
AcyI GRCGYC 1 cut(s) 63
AfaI GTAC 2 cut(s) 104, 173
AfiI CCNNNNNNNGG 1 cut(s) 45
AflIII ACRYGT 1 cut(s) 168
AgsI TTSAA 1 cut(s) 94
AjiI CACGTC 2 cut(s) 169, 293
AjnI CCWGG 1 cut(s) 132
AluBI AGCT 2 cut(s) 139, 217
AluI AGCT 2 cut(s) 139, 217
AoxI GGCC 1 cut(s) 196
ApoI RAATTY 1 cut(s) 255
AseI ATTAAT 1 cut(s) 207
AspLEI GCGC 1 cut(s) 120
AsuC2I CCSGG 1 cut(s) 46
BalI TGGCCA 1 cut(s) 198
BccI CCATC 2 cut(s) 188, 239
BciT130I CCWGG 1 cut(s) 134
BcnI CCSGG 1 cut(s) 46
BfaI CTAG 1 cut(s) 298
BisI GCNGC 1 cut(s) 39
BlsI GCNGC 1 cut(s) 40
Bme1390I CCNGG 2 cut(s) 46, 134
BmgBI CACGTC 2 cut(s) 169, 293
BmrFI CCNGG 2 cut(s) 46, 134
BpmI CTGGAG 2 cut(s) 116, 212
BpuMI CCSGG 1 cut(s) 46
BsaHI GRCGYC 1 cut(s) 63
BsaJI CCNNGG 1 cut(s) 199
Bsc4I CCNNNNNNNGG 1 cut(s) 45
Bse1I ACTGG 1 cut(s) 229
Bse3DI GCAATG 1 cut(s) 270
BseBI CCWGG 1 cut(s) 134
BseDI CCNNGG 1 cut(s) 199
BseGI GGATG 2 cut(s) 117, 256
BseLI CCNNNNNNNGG 1 cut(s) 45
BseMI GCAATG 1 cut(s) 270
BseNI ACTGG 1 cut(s) 229
BsgI GTGCAG 1 cut(s) 207
BshFI GGCC 1 cut(s) 198
BsiSI CCGG 1 cut(s) 45
BslI CCNNNNNNNGG 1 cut(s) 45
BsnI GGCC 1 cut(s) 198
Bsp19I CCATGG 1 cut(s) 199
BspACI CCGC 3 cut(s) 36, 39, 128
BspANI GGCC 1 cut(s) 198
BsrBI CCGCTC 1 cut(s) 130
BsrDI GCAATG 1 cut(s) 270
BsrI ACTGG 1 cut(s) 229
BssECI CCNNGG 1 cut(s) 199
BssNI GRCGYC 1 cut(s) 63
BssT1I CCWWGG 1 cut(s) 199
Bst2UI CCWGG 1 cut(s) 134
Bst4CI ACNGT 1 cut(s) 155
BstACI GRCGYC 1 cut(s) 63
BstDSI CCRYGG 1 cut(s) 199
BstF5I GGATG 2 cut(s) 117, 256
BstHHI GCGC 1 cut(s) 120
BstMWI GCNNNNNNNGC 1 cut(s) 136
BstNI CCWGG 1 cut(s) 134
BstSCI CCNGG 2 cut(s) 44, 132
BsuRI GGCC 1 cut(s) 198
BtgI CCRYGG 1 cut(s) 199
BtrI CACGTC 2 cut(s) 169, 293
BtsCI GGATG 2 cut(s) 117, 256
BtsI GCAGTG 1 cut(s) 219
BtsIMutI CAGTG 1 cut(s) 219
CfoI GCGC 1 cut(s) 120
Csp6I GTAC 2 cut(s) 103, 172
CviAII CATG 1 cut(s) 200
CviJI RGCY 5 cut(s) 31, 139, 198, 217, 297
CviKI_1 RGCY 5 cut(s) 31, 139, 198, 217, 297
CviQI GTAC 2 cut(s) 103, 172
EaeI YGGCCR 1 cut(s) 196
Eco130I CCWWGG 1 cut(s) 199
Eco57I CTGAAG 1 cut(s) 91
EcoRII CCWGG 1 cut(s) 132
EcoT14I CCWWGG 1 cut(s) 199
ErhI CCWWGG 1 cut(s) 199
FaeI CATG 1 cut(s) 203
FaiI YATR 1 cut(s) 201
FatI CATG 1 cut(s) 199
FblI GTMKAC 1 cut(s) 289
Fnu4HI GCNGC 1 cut(s) 39
FokI GGATG 2 cut(s) 124, 263
Fsp4HI GCNGC 1 cut(s) 39
FspBI CTAG 1 cut(s) 298
GlaI GCGC 1 cut(s) 119
GluI GCNGC 1 cut(s) 39
GsuI CTGGAG 2 cut(s) 116, 212
HaeIII GGCC 1 cut(s) 198
HapII CCGG 1 cut(s) 45
HhaI GCGC 1 cut(s) 120
Hin1I GRCGYC 1 cut(s) 63
Hin1II CATG 1 cut(s) 203
Hin6I GCGC 1 cut(s) 118
HinP1I GCGC 1 cut(s) 118
HincII GTYRAC 3 cut(s) 67, 277, 290
HindII GTYRAC 3 cut(s) 67, 277, 290
HinfI GANTC 1 cut(s) 183
HpaII CCGG 1 cut(s) 45
Hpy166II GTNNAC 5 cut(s) 67, 151, 172, 277, 290
Hpy188I TCNGA 1 cut(s) 182
Hpy188III TCNNGA 3 cut(s) 79, 109, 234
Hpy8I GTNNAC 5 cut(s) 67, 151, 172, 277, 290
Hpy99I CGWCG 3 cut(s) 65, 170, 294
HpyCH4III ACNGT 1 cut(s) 155
HpyCH4IV ACGT 4 cut(s) 63, 168, 273, 292
HpyCH4V TGCA 3 cut(s) 224, 263, 271
HpyF10VI GCNNNNNNNGC 1 cut(s) 136
HpySE526I ACGT 4 cut(s) 63, 168, 273, 292
Hsp92I GRCGYC 1 cut(s) 63
Hsp92II CATG 1 cut(s) 203
HspAI GCGC 1 cut(s) 118
LmnI GCTCC 2 cut(s) 135, 136
LpnPI CCDG 7 cut(s) 58, 94, 98, 119, 146, 219, 242
MaeI CTAG 1 cut(s) 298
MaeII ACGT 4 cut(s) 63, 168, 273, 292
MbiI CCGCTC 1 cut(s) 130
MlsI TGGCCA 1 cut(s) 198
MluCI AATT 2 cut(s) 89, 255
MluNI TGGCCA 1 cut(s) 198
MnlI CCTC 5 cut(s) 52, 80, 108, 197, 275
Mox20I TGGCCA 1 cut(s) 198
MscI TGGCCA 1 cut(s) 198
MseI TTAA 1 cut(s) 207
Msp20I TGGCCA 1 cut(s) 198
MspI CCGG 1 cut(s) 45
MspR9I CCNGG 2 cut(s) 46, 134
MvaI CCWGG 1 cut(s) 134
MwoI GCNNNNNNNGC 1 cut(s) 136
NciI CCSGG 1 cut(s) 46
NcoI CCATGG 1 cut(s) 199
NlaIII CATG 1 cut(s) 203
PfeI GAWTC 1 cut(s) 183
PfoI TCCNGGA 1 cut(s) 132
PkrI GCNGC 1 cut(s) 40
PshBI ATTAAT 1 cut(s) 207
Psp6I CCWGG 1 cut(s) 132
PspGI CCWGG 1 cut(s) 132
RsaI GTAC 2 cut(s) 104, 173
RsaNI GTAC 2 cut(s) 103, 172
SalI GTCGAC 1 cut(s) 288
SaqAI TTAA 1 cut(s) 207
SatI GCNGC 1 cut(s) 39
ScrFI CCNGG 2 cut(s) 46, 134
SetI ASST 8 cut(s) 66, 72, 141, 171, 219, 276, 286, 295
Sse9I AATT 2 cut(s) 89, 255
SsiI CCGC 3 cut(s) 36, 39, 128
SspMI CTAG 1 cut(s) 298
StyD4I CCNGG 2 cut(s) 44, 132
StyI CCWWGG 1 cut(s) 199
TaaI ACNGT 1 cut(s) 155
TaiI ACGT 4 cut(s) 66, 171, 276, 295
TaqI TCGA 1 cut(s) 289
TasI AATT 2 cut(s) 89, 255
TatI WGTACW 1 cut(s) 102
TauI GCSGC 1 cut(s) 41
TfiI GAWTC 1 cut(s) 183
Tru1I TTAA 1 cut(s) 207
Tru9I TTAA 1 cut(s) 207
TscAI CASTG 1 cut(s) 226
TspDTI ATGAA 3 cut(s) 17, 254, 267
TspGWI ACGGA 1 cut(s) 294
TspRI CASTG 1 cut(s) 226
VspI ATTAAT 1 cut(s) 207
XapI RAATTY 1 cut(s) 255
XmiI GTMKAC 1 cut(s) 289
XspI CTAG 1 cut(s) 298
ZraI GACGTC 1 cut(s) 64
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.