RchiOBHm_Chr2g0095681

Belongs to the RNase T2 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
2
OG0019650
Physical Location & Seq
Forward (+)
8615730 .. 8616191
462 bp
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UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 462 bp
ATGATGTCTTCATTTCTCACCATTTTTCTCTGTTTTCAACTGCTCCTAGCAGTGCCAAATCCACTTCGAGCGAAAATTTCAGCTCCTGATTCCTTCCTGTTGGTAATTGTTTGGCCCAATACCTTCTGTCTTTTTCAGAGCAATCCATGTCAACAGCTTCCACAATCTTTTACGTTACACGGATTGTGGCCGCAGGCGGAAGGTTCCTCGTTGAAATGTACGAGTGTACCAATGATTGATAGCATCTTAAAAGGCAACAAAGATGATTTGGAAAGGTATTGGCCAAACTTGAAGCACACAAAGTTCGATGAGAGTAAAAAATTCTGGATTAGTGAGTGGATTAAACATGGTAGCTGCTCCGCAAAGACTCCTGCTAATTACTTGAGCCTGGTTTTTGATCTAATGAAGAAAATCAAGAAATTTGATGTGAAAAAGATATTTGAAAAGCACGGTAAGATATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Ribonuclease_T2

Protein Analysis

153

Amino Acids

17.63

Weight (kDa)

9.34

Isoelectric Point (pI)

35.36

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ribonuclease_T2 PF00445 30 - 150 5.1e-23 Ribonuclease T2 family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0019650)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g10040
rosa_chinensis RchiOBHm_Chr2g0095681
rosa_laevigata RLG00000016585
rosa_multiflora Rmu_sc0001309.1_g000009 Rmu_ssc0000215.1_g000027
rosa_samantha Rh2AG108600 Rh2DG102100

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 3 cut(s) 191, 197, 360
AcoI YGGCCR 2 cut(s) 188, 281
AcsI RAATTY 3 cut(s) 75, 320, 419
AfaI GTAC 2 cut(s) 220, 228
AgsI TTSAA 4 cut(s) 38, 214, 292, 443
AjnI CCWGG 1 cut(s) 387
AluBI AGCT 3 cut(s) 83, 157, 354
AluI AGCT 3 cut(s) 83, 157, 354
AlwNI CAGNNNCTG 1 cut(s) 86
AoxI GGCC 3 cut(s) 113, 188, 281
ApeKI GCWGC 1 cut(s) 354
ApoI RAATTY 3 cut(s) 75, 320, 419
AspS9I GGNCC 1 cut(s) 114
AsuHPI GGTGA 1 cut(s) 10
BaeI ACNNNNGTAYC 2 cut(s) 210, 243
BalI TGGCCA 1 cut(s) 283
BbvI GCAGC 1 cut(s) 341
BciT130I CCWGG 1 cut(s) 389
BfaI CTAG 1 cut(s) 47
BisI GCNGC 2 cut(s) 191, 355
BlsI GCNGC 2 cut(s) 192, 356
Bme1390I CCNGG 1 cut(s) 389
BmgT120I GGNCC 1 cut(s) 114
BmiI GGNNCC 1 cut(s) 205
BmrFI CCNGG 1 cut(s) 389
BmsI GCATC 1 cut(s) 252
BpuEI CTTGAG 1 cut(s) 403
BseBI CCWGG 1 cut(s) 389
BseXI GCAGC 1 cut(s) 341
BshFI GGCC 3 cut(s) 115, 190, 283
BsnI GGCC 3 cut(s) 115, 190, 283
Bsp143I GATC 1 cut(s) 397
BspACI CCGC 3 cut(s) 191, 197, 360
BspANI GGCC 3 cut(s) 115, 190, 283
BspLI GGNNCC 1 cut(s) 205
BssMI GATC 1 cut(s) 397
Bst2UI CCWGG 1 cut(s) 389
Bst4CI ACNGT 1 cut(s) 452
BstC8I GCNNGC 1 cut(s) 195
BstKTI GATC 1 cut(s) 400
BstMBI GATC 1 cut(s) 397
BstNI CCWGG 1 cut(s) 389
BstSCI CCNGG 1 cut(s) 387
BstV1I GCAGC 1 cut(s) 341
BsuRI GGCC 3 cut(s) 115, 190, 283
BtsI GCAGTG 1 cut(s) 57
BtsIMutI CAGTG 1 cut(s) 57
Cac8I GCNNGC 1 cut(s) 195
CaiI CAGNNNCTG 1 cut(s) 86
Cfr13I GGNCC 1 cut(s) 114
Csp6I GTAC 2 cut(s) 219, 227
CviAII CATG 2 cut(s) 147, 347
CviJI RGCY 7 cut(s) 83, 115, 157, 190, 283, 354, 387
CviKI_1 RGCY 7 cut(s) 83, 115, 157, 190, 283, 354, 387
CviQI GTAC 2 cut(s) 219, 227
DpnI GATC 1 cut(s) 399
DpnII GATC 1 cut(s) 397
EaeI YGGCCR 2 cut(s) 188, 281
EciI GGCGGA 1 cut(s) 212
EcoRII CCWGG 1 cut(s) 387
FaeI CATG 2 cut(s) 150, 350
FaiI YATR 3 cut(s) 148, 348, 460
FatI CATG 2 cut(s) 146, 346
Fnu4HI GCNGC 2 cut(s) 191, 355
Fsp4HI GCNGC 2 cut(s) 191, 355
FspBI CTAG 1 cut(s) 47
GluI GCNGC 2 cut(s) 191, 355
HaeIII GGCC 3 cut(s) 115, 190, 283
Hin1II CATG 2 cut(s) 150, 350
HincII GTYRAC 1 cut(s) 152
HindII GTYRAC 1 cut(s) 152
HinfI GANTC 2 cut(s) 89, 367
HphI GGTGA 1 cut(s) 10
Hpy166II GTNNAC 2 cut(s) 152, 227
Hpy188I TCNGA 1 cut(s) 138
Hpy188III TCNNGA 3 cut(s) 86, 325, 415
Hpy8I GTNNAC 2 cut(s) 152, 227
HpyAV CCTTC 3 cut(s) 103, 133, 194
HpyCH4III ACNGT 1 cut(s) 452
HpyCH4IV ACGT 1 cut(s) 173
HpySE526I ACGT 1 cut(s) 173
Hsp92II CATG 2 cut(s) 150, 350
Kzo9I GATC 1 cut(s) 397
LmnI GCTCC 3 cut(s) 48, 88, 362
LpnPI CCDG 7 cut(s) 99, 110, 179, 310, 374, 384, 401
Lsp1109I GCAGC 1 cut(s) 341
LweI GCATC 1 cut(s) 252
MaeI CTAG 1 cut(s) 47
MaeII ACGT 1 cut(s) 173
MaeIII GTNAC 1 cut(s) 174
MalI GATC 1 cut(s) 399
MboI GATC 1 cut(s) 397
MboII GAAGA 1 cut(s) 418
MlsI TGGCCA 1 cut(s) 283
MluCI AATT 5 cut(s) 75, 105, 320, 376, 419
MluNI TGGCCA 1 cut(s) 283
MlyI GAGTC 1 cut(s) 361
MnlI CCTC 1 cut(s) 217
Mox20I TGGCCA 1 cut(s) 283
MscI TGGCCA 1 cut(s) 283
MseI TTAA 2 cut(s) 248, 342
Msp20I TGGCCA 1 cut(s) 283
MspR9I CCNGG 1 cut(s) 389
MvaI CCWGG 1 cut(s) 389
NdeII GATC 1 cut(s) 397
NlaIII CATG 2 cut(s) 150, 350
NlaIV GGNNCC 1 cut(s) 205
PfeI GAWTC 1 cut(s) 89
PkrI GCNGC 2 cut(s) 192, 356
PleI GAGTC 1 cut(s) 361
PpsI GAGTC 1 cut(s) 361
Psp6I CCWGG 1 cut(s) 387
PspGI CCWGG 1 cut(s) 387
PspN4I GGNNCC 1 cut(s) 205
PspPI GGNCC 1 cut(s) 114
PstNI CAGNNNCTG 1 cut(s) 86
RsaI GTAC 2 cut(s) 220, 228
RsaNI GTAC 2 cut(s) 219, 227
SaqAI TTAA 2 cut(s) 248, 342
SatI GCNGC 2 cut(s) 191, 355
Sau3AI GATC 1 cut(s) 397
Sau96I GGNCC 1 cut(s) 114
SchI GAGTC 1 cut(s) 361
ScrFI CCNGG 1 cut(s) 389
SetI ASST 7 cut(s) 85, 125, 159, 176, 205, 278, 356
SfaNI GCATC 1 cut(s) 252
SmlI CTYRAG 1 cut(s) 382
SmoI CTYRAG 1 cut(s) 382
Sse9I AATT 5 cut(s) 75, 105, 320, 376, 419
SsiI CCGC 3 cut(s) 191, 197, 360
SspMI CTAG 1 cut(s) 47
StyD4I CCNGG 1 cut(s) 387
TaaI ACNGT 1 cut(s) 452
TaiI ACGT 1 cut(s) 176
TaqI TCGA 2 cut(s) 67, 306
TasI AATT 5 cut(s) 75, 105, 320, 376, 419
TauI GCSGC 1 cut(s) 193
TfiI GAWTC 1 cut(s) 89
Tru1I TTAA 2 cut(s) 248, 342
Tru9I TTAA 2 cut(s) 248, 342
TscAI CASTG 1 cut(s) 57
TseI GCWGC 1 cut(s) 354
TspDTI ATGAA 1 cut(s) 419
TspGWI ACGGA 1 cut(s) 195
TspRI CASTG 1 cut(s) 57
XapI RAATTY 3 cut(s) 75, 320, 419
XspI CTAG 1 cut(s) 47
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.