RchiOBHm_Chr2g0098891

protein synthesis. May act as a fidelity factor of the translation reaction, by catalyzing a one- codon backward translocation of tRNAs on improperly translocated ribosomes

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
2
OG0015011
Physical Location & Seq
Reverse (-)
11194101 .. 11196201
2101 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1959 bp
ATGGCCGCAGACCTCTGTTCCCGTTCTCCGGCCTTCCTCCTCTCCACAACCGCCACCACCACTCCCCAACGCTCCCAACCCTCCACCGCCTTCTTACCCTTCATCTCCCTCTCCCCCTCCAAGCCCTTCCTCCACAAAACCCACCTCCGCCCCAAGCCCAAGTTCCACGTCCTCTCTCAGGCCCCCCAGCCCACTTCCCAGCTCGGCCACGACCGCCTCTCCAAGGTCCCCATCTCCCACATCCGCAACTTCTGCATCATCGCTCACATCGACCACGGCAAGTCCACTCTCGCCGACAAGCTTCTAGAATCCACCGGAACAGTCCAGAAGCGTGAAATGAAGGAGCAGTTTCTGGACAACATGGACCTGGAGCGCGAGAGAGGCATCACCATCAAGTTACAGATAGCTCGGATGAGGTACAGTTACAAAACCGGTGAACCCTTCTGCCTGAACTTGATCGACACTCCTGGACATGTCGACTTCAGCTACGAGGTTTCGAGGTCTTTGGCTGCCTGTGAAGGTGCTTTGTTAGTTGTCGATGCTTCGCAGGGTGTCGAAGCGCAGACATTGGCGAATGTTTATCTTGCATTGGAGAATAACTTGGAGATTATTCCTGTTTTGAATAAGATTGATCTTCCTGGGGCGGACCCGGATACTGTAATGAAGGAGATTGAGGAGGTTATTGGGTTGGATTGTAGTAATGCGATACTCTGTTCTGCTAAGGAAGGGATTGGGATTAGTGAGATTTTGGATGCCATTGTTGAGAGGGTGCCTCCACCTGTTGACACTGCTGACAAGCCGCTGAGGGCTTTGATTTTTGATAGTTATTATGATCCTTATAGGGGTGTGATTGTGTATTTTAGGGTGATTGATGGGAAGATAAAGAAGGGGGATAGGGTTTATTTTATGGCTAGCGGGAAGGACTACTATGCTGATGACCTTGGGGTTTTGTCTCCGACTCAGTTTCAAGTGGGGGAGCTTTATGCTGGTGAGGTGGGGTTTCTTTCGGCTTCTATAAGATCGGTGGCCGATGCGAGAGTTGGGGATACGATCACGCATTATGGTAGGAAGGCGGAGAGTTCATTGCCGGGTTATGAGGAGGCTACTCCAATGGTGTTCTGTGGGATGTTCCCTGTGGATGCAGACCAGTTTCCGGACTTGAGGGATGCACTTGAGAAGCTGCAGCTTAATGATGCTGCTTTGCAGTTTGAGCCAGAGAGTTCGAGTGCTATGGGTTTTGGGTTTAGATGTGGGTTCTTGGGGCTTCTGCATATGGAAATCGTGCAGGAAAGGCTGGAGAGAGAGTACAATTTGAGCTTGATTACTACTGCACCTAGTGTTGTGTATAAAGTGAACTGCGTAAATGGGGAAGTTGTTGAATGCTCGAATCCATCTTTGCTTCCTGAACCTGGCAAAAGGAAGTCGATTGAGGAGCCTATTGTGAAGATTGAGATGCTTACACCGAAAGAGTATATTGGCCCCCTTATGGAATTGGCGCAGGACAGGAGAGCAGAGTTTAAGGAAATGAAGTTTATTGCTGAGAATAGGGCATCACTCACTTATGAATTACCCCTAGCTGAGATGGTAGGTGATTTTTTTGACCAGCTCAAATCCAGAAGTAAGGGTTATGCTAGCATGGAGTATACTTTACTTGGGTACAAAGAAAGTGATCTAATAAGACTTGATATTCAAATTAATGGTGATCCTGTAGAGCCATTGGCTACTATTGTTCACAAGGATAAGGCATATGCTGTAGGGAGGGCGTTAACACAAAAGCTGAAGGAGCTTATACCAAGACAGATGTTTAAAGTACCGATTCAAGCATGCATAGGTACGAAAGTGATTGCCAGTGAAGCTTTATCGGCAATTAGAAAGGATGTTTTAGCCAAATGCTATGGCGGAGACATTTCAAGGAAAAGAAATTGCTTAAGAAACAGGCTGAGGGGAAGAAAAGAATGA

Protein Analysis

652

Amino Acids

72.55

Weight (kDa)

5.91

Isoelectric Point (pI)

37.82

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GTP_EFTU PF00009 80 - 259 2.8e-56 Elongation factor Tu GTP binding domain
GTP_EFTU_D2 PF03144 282 - 352 3.3e-10 Elongation factor Tu domain 2
EFG_C PF00679 476 - 558 5.6e-21 Elongation factor G C-terminus
LepA_C PF06421 565 - 651 1.5e-32 GTP-binding protein LepA C-terminus
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0015011)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G08650 AT5G08650
fragaria_vesca FvH4_1g11350
malus_domestica MD02G1127200.v1.1
prunus_persica Prupe.7G172400_v2.0.a1 Prupe.7G172400_v2.0.a1
pyrus_communis pycom02g09930
rosa_chinensis RchiOBHm_Chr2g0098891
rosa_laevigata RLG00000016831
rosa_multiflora Rmu_sc0008564.1_g000017
rosa_roxburghii Rroxscaffold_2G00143650
rosa_samantha Rh2AG123500 Rh2BG127200 Rh2CG128100 Rh2DG128700
rosa_wichuraiana Rw2G009520

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 769
AccI GTMKAC 2 cut(s) 477, 1643
AccII CGCG 1 cut(s) 375
AccIII TCCGGA 1 cut(s) 1153
AclWI GGATC 2 cut(s) 827, 1697
AcoI YGGCCR 3 cut(s) 3, 205, 1026
AcuI CTGAAG 2 cut(s) 466, 1799
AdeI CACNNNGTG 1 cut(s) 1337
AfaI GTAC 5 cut(s) 419, 1307, 1658, 1812, 1834
AfiI CCNNNNNNNGG 7 cut(s) 28, 178, 223, 842, 1153, 1409, 1486
AflII CTTAAG 1 cut(s) 1927
AflIII ACRYGT 1 cut(s) 472
AgeI ACCGGT 1 cut(s) 431
AgsI TTSAA 6 cut(s) 622, 968, 1379, 1691, 1820, 1911
AjiI CACGTC 1 cut(s) 169
AjnI CCWGG 4 cut(s) 366, 466, 637, 1408
AjuI GAANNNNNNNTTGG 2 cut(s) 146, 178
Alw26I GTCTC 2 cut(s) 957, 1896
AlwI GGATC 2 cut(s) 827, 1697
AlwNI CAGNNNCTG 1 cut(s) 352
Aor13HI TCCGGA 1 cut(s) 1153
AoxI GGCC 6 cut(s) 3, 30, 180, 205, 1026, 1477
ApeKI GCWGC 4 cut(s) 509, 1180, 1183, 1196
AseI ATTAAT 1 cut(s) 1695
AsiGI ACCGGT 1 cut(s) 431
AspLEI GCGC 3 cut(s) 375, 562, 1498
AspS9I GGNCC 5 cut(s) 181, 226, 364, 646, 1478
AsuC2I CCSGG 2 cut(s) 650, 1089
AsuHPI GGTGA 6 cut(s) 379, 446, 877, 1001, 1601, 1712
AsuNHI GCTAGC 2 cut(s) 911, 1631
AvaII GGWCC 3 cut(s) 226, 364, 646
BaeI ACNNNNGTAYC 2 cut(s) 1824, 1857
BanI GGYRCC 1 cut(s) 769
BbvCI CCTCAGC 2 cut(s) 803, 1940
BbvI GCAGC 4 cut(s) 496, 1167, 1183, 1195
BccI CCATC 5 cut(s) 239, 398, 866, 1399, 1576
BceAI ACGGC 1 cut(s) 292
BciT130I CCWGG 4 cut(s) 368, 468, 639, 1410
BciVI GTATCC 2 cut(s) 646, 1039
BcnI CCSGG 2 cut(s) 650, 1089
BcoDI GTCTC 2 cut(s) 957, 1896
BfaI CTAG 5 cut(s) 305, 912, 1335, 1574, 1632
BfmI CTRYAG 3 cut(s) 1181, 1707, 1752
BfrI CTTAAG 1 cut(s) 1927
BfuI GTATCC 2 cut(s) 646, 1039
BisI GCNGC 6 cut(s) 6, 510, 800, 1181, 1184, 1197
BlsI GCNGC 6 cut(s) 7, 511, 801, 1182, 1185, 1198
Bme1390I CCNGG 6 cut(s) 368, 468, 639, 650, 1089, 1410
Bme18I GGWCC 3 cut(s) 226, 364, 646
BmgBI CACGTC 1 cut(s) 169
BmgT120I GGNCC 5 cut(s) 181, 226, 364, 646, 1478
BmiI GGNNCC 6 cut(s) 183, 228, 648, 771, 1434, 1480
BmrFI CCNGG 6 cut(s) 368, 468, 639, 650, 1089, 1410
BmtI GCTAGC 2 cut(s) 915, 1635
BplI GAGNNNNNCTC 2 cut(s) 757, 789
BpmI CTGGAG 2 cut(s) 389, 1316
Bpu10I CCTNAGC 3 cut(s) 720, 803, 1940
BpuEI CTTGAG 2 cut(s) 1180, 1193
BpuMI CCSGG 2 cut(s) 650, 1089
BsaJI CCNNGG 4 cut(s) 222, 274, 638, 940
BsaWI WCCGGW 3 cut(s) 314, 431, 1153
BsaXI ACNNNNNCTCC 6 cut(s) 46, 76, 203, 233, 1289, 1319
Bsc4I CCNNNNNNNGG 7 cut(s) 28, 178, 223, 842, 1153, 1409, 1486
Bse118I RCCGGY 1 cut(s) 431
Bse1I ACTGG 2 cut(s) 1147, 1848
Bse3DI GCAATG 1 cut(s) 1082
BseAI TCCGGA 1 cut(s) 1153
BseBI CCWGG 4 cut(s) 368, 468, 639, 1410
BseDI CCNNGG 4 cut(s) 222, 274, 638, 940
BseGI GGATG 7 cut(s) 240, 417, 757, 1131, 1144, 1171, 1882
BseLI CCNNNNNNNGG 7 cut(s) 28, 178, 223, 842, 1153, 1409, 1486
BseMI GCAATG 1 cut(s) 1082
BseMII CTCAG 6 cut(s) 191, 794, 974, 1530, 1569, 1931
BseNI ACTGG 2 cut(s) 1147, 1848
BseRI GAGGAG 4 cut(s) 29, 689, 1112, 1445
BseXI GCAGC 4 cut(s) 496, 1167, 1183, 1195
BseYI CCCAGC 2 cut(s) 186, 198
BsgI GTGCAG 2 cut(s) 1304, 1314
Bsh1236I CGCG 1 cut(s) 375
Bsh1285I CGRYCG 1 cut(s) 214
BshFI GGCC 6 cut(s) 5, 32, 182, 207, 1028, 1479
BshNI GGYRCC 1 cut(s) 769
BshTI ACCGGT 1 cut(s) 431
BsiEI CGRYCG 1 cut(s) 214
BsiSI CCGG 6 cut(s) 29, 315, 432, 650, 1088, 1154
BslFI GGGAC 1 cut(s) 212
BslI CCNNNNNNNGG 7 cut(s) 28, 178, 223, 842, 1153, 1409, 1486
BsmAI GTCTC 2 cut(s) 957, 1896
BsmFI GGGAC 1 cut(s) 212
BsmI GAATGC 1 cut(s) 1385
BsnI GGCC 6 cut(s) 5, 32, 182, 207, 1028, 1479
Bsp13I TCCGGA 1 cut(s) 1153
Bsp143I GATC 7 cut(s) 456, 631, 832, 1019, 1050, 1669, 1702
BspANI GGCC 6 cut(s) 5, 32, 182, 207, 1028, 1479
BspCNI CTCAG 6 cut(s) 190, 795, 973, 1531, 1570, 1932
BspEI TCCGGA 1 cut(s) 1153
BspFNI CGCG 1 cut(s) 375
BspLI GGNNCC 6 cut(s) 183, 228, 648, 771, 1434, 1480
BspMAI CTGCAG 1 cut(s) 1185
BspOI GCTAGC 2 cut(s) 915, 1635
BspPI GGATC 2 cut(s) 827, 1697
BspT107I GGYRCC 1 cut(s) 769
BspTI CTTAAG 1 cut(s) 1927
BsrDI GCAATG 1 cut(s) 1082
BsrFI RCCGGY 1 cut(s) 431
BsrI ACTGG 2 cut(s) 1147, 1848
BssAI RCCGGY 1 cut(s) 431
BssECI CCNNGG 4 cut(s) 222, 274, 638, 940
BssMI GATC 7 cut(s) 456, 631, 832, 1019, 1050, 1669, 1702
BssNAI GTATAC 1 cut(s) 1644
BssT1I CCWWGG 2 cut(s) 222, 940
Bst1107I GTATAC 1 cut(s) 1644
Bst2UI CCWGG 4 cut(s) 368, 468, 639, 1410
Bst4CI ACNGT 3 cut(s) 322, 422, 658
BstAFI CTTAAG 1 cut(s) 1927
BstAPI GCANNNNNTGC 1 cut(s) 252
BstC8I GCNNGC 3 cut(s) 913, 1633, 1825
BstDEI CTNAG 7 cut(s) 177, 720, 803, 960, 1539, 1578, 1940
BstDSI CCRYGG 1 cut(s) 274
BstENI CCTNNNNNAGG 2 cut(s) 176, 221
BstF5I GGATG 7 cut(s) 240, 417, 757, 1131, 1144, 1171, 1882
BstFNI CGCG 1 cut(s) 375
BstHHI GCGC 3 cut(s) 375, 562, 1498
BstKTI GATC 7 cut(s) 459, 634, 835, 1022, 1053, 1672, 1705
BstMAI GTCTC 2 cut(s) 957, 1896
BstMBI GATC 7 cut(s) 456, 631, 832, 1019, 1050, 1669, 1702
BstMCI CGRYCG 1 cut(s) 214
BstMWI GCNNNNNNNGC 8 cut(s) 213, 252, 381, 1210, 1291, 1783, 1853, 1862
BstNI CCWGG 4 cut(s) 368, 468, 639, 1410
BstNSI RCATGY 2 cut(s) 476, 1827
BstSCI CCNGG 6 cut(s) 366, 466, 637, 648, 1087, 1408
BstSFI CTRYAG 3 cut(s) 1181, 1707, 1752
BstUI CGCG 1 cut(s) 375
BstV1I GCAGC 4 cut(s) 496, 1167, 1183, 1195
BstZ17I GTATAC 1 cut(s) 1644
BsuI GTATCC 2 cut(s) 646, 1039
BsuRI GGCC 6 cut(s) 5, 32, 182, 207, 1028, 1479
BtgI CCRYGG 1 cut(s) 274
BtgZI GCGATG 1 cut(s) 244
BtrI CACGTC 1 cut(s) 169
BtsCI GGATG 7 cut(s) 240, 417, 757, 1131, 1144, 1171, 1882
BtsI GCAGTG 1 cut(s) 786
BtsIMutI CAGTG 2 cut(s) 786, 1855
Cac8I GCNNGC 3 cut(s) 913, 1633, 1825
CaiI CAGNNNCTG 1 cut(s) 352
CfoI GCGC 3 cut(s) 375, 562, 1498
Cfr10I RCCGGY 1 cut(s) 431
Cfr13I GGNCC 5 cut(s) 181, 226, 364, 646, 1478
Csp6I GTAC 5 cut(s) 418, 1306, 1657, 1811, 1833
CspAI ACCGGT 1 cut(s) 431
CviAII CATG 4 cut(s) 361, 473, 1636, 1824
CviQI GTAC 5 cut(s) 418, 1306, 1657, 1811, 1833
DdeI CTNAG 7 cut(s) 177, 720, 803, 960, 1539, 1578, 1940
DpnI GATC 7 cut(s) 458, 633, 834, 1021, 1052, 1671, 1704
DpnII GATC 7 cut(s) 456, 631, 832, 1019, 1050, 1669, 1702
DraI TTTAAA 1 cut(s) 1807
DraIII CACNNNGTG 1 cut(s) 1337
EaeI YGGCCR 3 cut(s) 3, 205, 1026
EciI GGCGGA 4 cut(s) 137, 659, 1088, 1914
Eco130I CCWWGG 2 cut(s) 222, 940
Eco47I GGWCC 3 cut(s) 226, 364, 646
Eco57I CTGAAG 2 cut(s) 466, 1799
EcoNI CCTNNNNNAGG 2 cut(s) 176, 221
EcoO109I RGGNCCY 2 cut(s) 181, 226
EcoRII CCWGG 4 cut(s) 366, 466, 637, 1408
EcoT14I CCWWGG 2 cut(s) 222, 940
EcoT22I ATGCAT 1 cut(s) 1829
ErhI CCWWGG 2 cut(s) 222, 940
FaeI CATG 4 cut(s) 364, 476, 1639, 1827
FaqI GGGAC 1 cut(s) 212
FatI CATG 4 cut(s) 360, 472, 1635, 1823
FauI CCCGC 1 cut(s) 908
FauNDI CATATG 2 cut(s) 1272, 1747
FblI GTMKAC 2 cut(s) 477, 1643
Fnu4HI GCNGC 6 cut(s) 6, 510, 800, 1181, 1184, 1197
FokI GGATG 7 cut(s) 227, 424, 764, 1138, 1151, 1178, 1889
Fsp4HI GCNGC 6 cut(s) 6, 510, 800, 1181, 1184, 1197
FspBI CTAG 5 cut(s) 305, 912, 1335, 1574, 1632
GlaI GCGC 3 cut(s) 374, 561, 1497
GluI GCNGC 6 cut(s) 6, 510, 800, 1181, 1184, 1197
GsaI CCCAGC 2 cut(s) 190, 202
GsuI CTGGAG 2 cut(s) 389, 1316
HaeIII GGCC 6 cut(s) 5, 32, 182, 207, 1028, 1479
HapII CCGG 6 cut(s) 29, 315, 432, 650, 1088, 1154
HhaI GCGC 3 cut(s) 375, 562, 1498
Hin1II CATG 4 cut(s) 364, 476, 1639, 1827
Hin6I GCGC 3 cut(s) 373, 560, 1496
HinP1I GCGC 3 cut(s) 373, 560, 1496
HincII GTYRAC 3 cut(s) 478, 784, 1767
HindII GTYRAC 3 cut(s) 478, 784, 1767
HindIII AAGCTT 2 cut(s) 299, 1854
HinfI GANTC 4 cut(s) 308, 958, 1387, 1816
HpaI GTTAAC 1 cut(s) 1767
HpaII CCGG 6 cut(s) 29, 315, 432, 650, 1088, 1154
HphI GGTGA 6 cut(s) 379, 446, 877, 1001, 1601, 1712
Hpy166II GTNNAC 8 cut(s) 285, 437, 478, 784, 1354, 1644, 1732, 1767
Hpy188I TCNGA 2 cut(s) 411, 957
Hpy188III TCNNGA 6 cut(s) 305, 325, 353, 1154, 1403, 1614
Hpy8I GTNNAC 8 cut(s) 285, 437, 478, 784, 1354, 1644, 1732, 1767
HpyCH4III ACNGT 3 cut(s) 322, 422, 658
HpyCH4IV ACGT 1 cut(s) 168
HpyF10VI GCNNNNNNNGC 8 cut(s) 213, 252, 381, 1210, 1291, 1783, 1853, 1862
HpyF3I CTNAG 7 cut(s) 177, 720, 803, 960, 1539, 1578, 1940
HpySE526I ACGT 1 cut(s) 168
Hsp92II CATG 4 cut(s) 364, 476, 1639, 1827
HspAI GCGC 3 cut(s) 373, 560, 1496
Kpn2I TCCGGA 1 cut(s) 1153
KspAI GTTAAC 1 cut(s) 1767
Kzo9I GATC 7 cut(s) 456, 631, 832, 1019, 1050, 1669, 1702
LmnI GCTCC 6 cut(s) 77, 343, 370, 976, 1432, 1783
Lsp1109I GCAGC 4 cut(s) 496, 1167, 1183, 1195
MaeI CTAG 5 cut(s) 305, 912, 1335, 1574, 1632
MaeII ACGT 1 cut(s) 168
MaeIII GTNAC 2 cut(s) 396, 422
MalI GATC 7 cut(s) 458, 633, 834, 1021, 1052, 1671, 1704
MboI GATC 7 cut(s) 456, 631, 832, 1019, 1050, 1669, 1702
MboII GAAGA 4 cut(s) 626, 889, 1456, 1959
MluCI AATT 6 cut(s) 1309, 1490, 1565, 1692, 1866, 1921
MlyI GAGTC 1 cut(s) 952
MmeI TCCRAC 2 cut(s) 669, 980
Mph1103I ATGCAT 1 cut(s) 1829
MroI TCCGGA 1 cut(s) 1153
MseI TTAA 6 cut(s) 1188, 1518, 1695, 1766, 1806, 1928
MspA1I CMGCKG 1 cut(s) 802
MspCI CTTAAG 1 cut(s) 1927
MspI CCGG 6 cut(s) 29, 315, 432, 650, 1088, 1154
MspR9I CCNGG 6 cut(s) 368, 468, 639, 650, 1089, 1410
Mva1269I GAATGC 1 cut(s) 1385
MvaI CCWGG 4 cut(s) 368, 468, 639, 1410
MvnI CGCG 1 cut(s) 375
MwoI GCNNNNNNNGC 8 cut(s) 213, 252, 381, 1210, 1291, 1783, 1853, 1862
NciI CCSGG 2 cut(s) 650, 1089
NdeI CATATG 2 cut(s) 1272, 1747
NdeII GATC 7 cut(s) 456, 631, 832, 1019, 1050, 1669, 1702
NheI GCTAGC 2 cut(s) 911, 1631
NlaIII CATG 4 cut(s) 364, 476, 1639, 1827
NlaIV GGNNCC 6 cut(s) 183, 228, 648, 771, 1434, 1480
NmeAIII GCCGAG 1 cut(s) 183
NsiI ATGCAT 1 cut(s) 1829
NspI RCATGY 2 cut(s) 476, 1827
PaeI GCATGC 1 cut(s) 1827
PciI ACATGT 1 cut(s) 472
PcsI WCGNNNNNNNCGW 1 cut(s) 267
PctI GAATGC 1 cut(s) 1385
PfeI GAWTC 3 cut(s) 308, 1387, 1816
PfoI TCCNGGA 1 cut(s) 466
PinAI ACCGGT 1 cut(s) 431
PkrI GCNGC 6 cut(s) 7, 511, 801, 1182, 1185, 1198
PleI GAGTC 1 cut(s) 952
PpsI GAGTC 1 cut(s) 952
PpuMI RGGWCCY 1 cut(s) 226
PscI ACATGT 1 cut(s) 472
PshBI ATTAAT 1 cut(s) 1695
Psp5II RGGWCCY 1 cut(s) 226
Psp6I CCWGG 4 cut(s) 366, 466, 637, 1408
PspFI CCCAGC 2 cut(s) 186, 198
PspGI CCWGG 4 cut(s) 366, 466, 637, 1408
PspN4I GGNNCC 6 cut(s) 183, 228, 648, 771, 1434, 1480
PspPI GGNCC 5 cut(s) 181, 226, 364, 646, 1478
PspPPI RGGWCCY 1 cut(s) 226
PstI CTGCAG 1 cut(s) 1185
PstNI CAGNNNCTG 1 cut(s) 352
RsaI GTAC 5 cut(s) 419, 1307, 1658, 1812, 1834
RsaNI GTAC 5 cut(s) 418, 1306, 1657, 1811, 1833
SalI GTCGAC 1 cut(s) 476
SaqAI TTAA 6 cut(s) 1188, 1518, 1695, 1766, 1806, 1928
SatI GCNGC 6 cut(s) 6, 510, 800, 1181, 1184, 1197
Sau3AI GATC 7 cut(s) 456, 631, 832, 1019, 1050, 1669, 1702
Sau96I GGNCC 5 cut(s) 181, 226, 364, 646, 1478
SchI GAGTC 1 cut(s) 952
ScrFI CCNGG 6 cut(s) 368, 468, 639, 650, 1089, 1410
SfcI CTRYAG 3 cut(s) 1181, 1707, 1752
SinI GGWCC 3 cut(s) 226, 364, 646
SmlI CTYRAG 3 cut(s) 1159, 1172, 1927
SmoI CTYRAG 3 cut(s) 1159, 1172, 1927
SphI GCATGC 1 cut(s) 1827
Sse9I AATT 6 cut(s) 1309, 1490, 1565, 1692, 1866, 1921
SspMI CTAG 5 cut(s) 305, 912, 1335, 1574, 1632
StyD4I CCNGG 6 cut(s) 366, 466, 637, 648, 1087, 1408
StyI CCWWGG 2 cut(s) 222, 940
TaaI ACNGT 3 cut(s) 322, 422, 658
TaiI ACGT 1 cut(s) 171
TaqI TCGA 9 cut(s) 270, 459, 477, 497, 537, 555, 1223, 1385, 1424
TasI AATT 6 cut(s) 1309, 1490, 1565, 1692, 1866, 1921
TatI WGTACW 1 cut(s) 1305
TauI GCSGC 2 cut(s) 8, 802
TfiI GAWTC 3 cut(s) 308, 1387, 1816
Tru1I TTAA 6 cut(s) 1188, 1518, 1695, 1766, 1806, 1928
Tru9I TTAA 6 cut(s) 1188, 1518, 1695, 1766, 1806, 1928
TscAI CASTG 2 cut(s) 793, 1855
TseI GCWGC 4 cut(s) 509, 1180, 1183, 1196
TspDTI ATGAA 6 cut(s) 91, 353, 677, 1071, 1541, 1578
TspRI CASTG 2 cut(s) 793, 1855
Vha464I CTTAAG 1 cut(s) 1927
VpaK11BI GGWCC 3 cut(s) 226, 364, 646
VspI ATTAAT 1 cut(s) 1695
XagI CCTNNNNNAGG 2 cut(s) 176, 221
XbaI TCTAGA 1 cut(s) 304
XceI RCATGY 2 cut(s) 476, 1827
XmiI GTMKAC 2 cut(s) 477, 1643
XspI CTAG 5 cut(s) 305, 912, 1335, 1574, 1632
Zsp2I ATGCAT 1 cut(s) 1829
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.