RchiOBHm_Chr2g0122911

phospholipase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
2
OG0017969
Physical Location & Seq
Reverse (-)
36056233 .. 36057912
1680 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1116 bp
ATGGGCAGCATAGCCACAAGATGGAGGCAACTGAGCGGCGAGAAGGATTGGGAGGGTCTATTGTACCCTCTGGACATTGATCTCCGCCGCTACATAATCCACTATGGTGAAAGGGCCGGAGCTAGTAGAGACGCTGTCATTGCTGAGCCAAAATCAAAGAACTTTGGACAACCCAGATATGCAAAGCGACATTTATTCTCTAAGGTGGGTTTGGAGATCGGGAATCCATACAAATATGTGGTGAGAAAGTATTTATATGCAGCCCCAAAACTACCGTCGTCCCCGGGAATGTCTTGTTTGTTGGGTTACGTTGCGGTGACAACAGATGAAGGAAGCAAAGTGTTGGGGAGGAGGGACGTTCTCATTTCGTTTAGAGGAGCAGTGCTGGGACATGAGAAGCTTGGTCATCCTACAGTGCACCTTCCTTGGCATACTTATTACACTAATGTTGACCTTCAATCCCCACACAACAAAACTAGCTGCAGAGATCAGGCTCAAGCTGCAGTAAGAGAACTATTGGGTCAATACAAGGACGAGGAAATCAGCGTTACTGCCACTGGCCATAGCATGGGTGCTGCATTCGCAGCTCAAACTGCAATAGACCTCGTTCACAATGGATTCAATATCATAAAACCTACAGGAATACCAGTGAAAGCGTGTCTTGTCACAGCCATCCTGTTTGCCTGCCCCCCTCGCCGCATTTTGGGATCCCAAAATGTAATTTCAGGAGGTCTAGAAGATCTTCACATCTTGCGTGTCCAAAACAAATATGATAACCATAATGAGCACCATGATGTTGGTATAGAGTTCAAAATCGACACTTTGAAATCACCCTACTTGAAGAATGCACTACAAAACGTGCACGACTTGCAGAATTACTTGCATGGAGTTGCAGGAAATCAAGGGGAAAGTGGTTTTAAGCTCGATGTTAATCGTGATCTCGCGCTAGTAAACAAATATGTGGATGGTCTCAAGGATGAATATAATGTAGTTGCGAAGTGGTGGACAGAGAAGAACAAGTCTATGGTCCAAATGGATGATGGCTCTTGGGTGTTGATAGATCATGAGAATGATGGTGATGAAGGAAACAAATTAAGCTTACAAAGCCGAATTTAA

Protein Analysis

371

Amino Acids

41.48

Weight (kDa)

8.18

Isoelectric Point (pI)

46.55

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lipase_3 PF01764 136 - 230 4.6e-10 Lipase (class 3)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0017969)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g25210
rosa_chinensis RchiOBHm_Chr2g0122911
rosa_laevigata RLG00000018725
rosa_multiflora Rmu_sc0001635.1_g000003
rosa_roxburghii Rroxscaffold_2G00120960
rosa_rugosa Rorug02G0243000
rosa_samantha Rh2AG302100 Rh2BG309600 Rh2CG288700 Rh2DG326100
rosa_wichuraiana Rw2G024000

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 2 cut(s) 21, 568
AccBSI CCGCTC 1 cut(s) 36
AccII CGCG 1 cut(s) 944
AciI CCGC 5 cut(s) 36, 85, 88, 314, 697
AclWI GGATC 2 cut(s) 702, 715
AcoI YGGCCR 1 cut(s) 559
AcsI RAATTY 1 cut(s) 1110
AfaI GTAC 1 cut(s) 65
AfiI CCNNNNNNNGG 3 cut(s) 21, 568, 703
AgsI TTSAA 5 cut(s) 458, 622, 811, 826, 841
AleI CACNNNNGTG 1 cut(s) 105
AluBI AGCT 7 cut(s) 122, 400, 480, 500, 587, 922, 1098
AluI AGCT 7 cut(s) 122, 400, 480, 500, 587, 922, 1098
Alw21I GWGCWC 3 cut(s) 420, 789, 864
Alw26I GTCTC 2 cut(s) 123, 974
Alw44I GTGCAC 2 cut(s) 416, 860
AlwI GGATC 2 cut(s) 702, 715
Ama87I CYCGRG 1 cut(s) 283
AoxI GGCC 2 cut(s) 114, 559
ApaLI GTGCAC 2 cut(s) 416, 860
ApeKI GCWGC 6 cut(s) 6, 260, 480, 500, 575, 584
ApoI RAATTY 1 cut(s) 1110
ArsI GACNNNNNNTTYG 2 cut(s) 260, 292
Asp700I GAANNNNTTC 1 cut(s) 741
AspLEI GCGC 1 cut(s) 946
AspS9I GGNCC 2 cut(s) 114, 1027
AsuC2I CCSGG 2 cut(s) 284, 285
AsuHPI GGTGA 5 cut(s) 119, 253, 328, 822, 1088
AvaI CYCGRG 1 cut(s) 283
AvaII GGWCC 1 cut(s) 1027
BaeGI GKGCMC 2 cut(s) 420, 864
BalI TGGCCA 1 cut(s) 561
BamHI GGATCC 1 cut(s) 707
Bbv12I GWGCWC 3 cut(s) 420, 789, 864
BbvI GCAGC 6 cut(s) 18, 272, 467, 487, 562, 596
BccI CCATC 5 cut(s) 15, 680, 959, 1034, 1067
BcnI CCSGG 2 cut(s) 284, 285
BcoDI GTCTC 2 cut(s) 123, 974
BfaI CTAG 4 cut(s) 123, 477, 734, 947
BfmI CTRYAG 4 cut(s) 411, 481, 501, 636
BglII AGATCT 1 cut(s) 739
BisI GCNGC 9 cut(s) 7, 37, 88, 261, 481, 501, 576, 585, 697
BlpI GCTNAGC 1 cut(s) 144
BlsI GCNGC 9 cut(s) 8, 38, 89, 262, 482, 502, 577, 586, 698
Bme1390I CCNGG 2 cut(s) 284, 285
Bme18I GGWCC 1 cut(s) 1027
BmeT110I CYCGRG 1 cut(s) 283
BmgT120I GGNCC 2 cut(s) 114, 1027
BmiI GGNNCC 1 cut(s) 709
BmrFI CCNGG 2 cut(s) 284, 285
Bpu1102I GCTNAGC 1 cut(s) 144
BpuEI CTTGAG 2 cut(s) 480, 956
BpuMI CCSGG 2 cut(s) 284, 285
BsaBI GATNNNNATC 1 cut(s) 930
BsaI GGTCTC 1 cut(s) 974
BsaJI CCNNGG 3 cut(s) 282, 283, 425
Bsc4I CCNNNNNNNGG 3 cut(s) 21, 568, 703
Bse1I ACTGG 2 cut(s) 562, 647
Bse3DI GCAATG 1 cut(s) 138
Bse8I GATNNNNATC 1 cut(s) 930
BseDI CCNNGG 3 cut(s) 282, 283, 425
BseGI GGATG 5 cut(s) 406, 672, 970, 982, 1042
BseJI GATNNNNATC 1 cut(s) 930
BseLI CCNNNNNNNGG 3 cut(s) 21, 568, 703
BseMI GCAATG 1 cut(s) 138
BseMII CTCAG 2 cut(s) 23, 135
BseNI ACTGG 2 cut(s) 562, 647
BseRI GAGGAG 2 cut(s) 364, 390
BseSI GKGCMC 2 cut(s) 420, 864
BseXI GCAGC 6 cut(s) 18, 272, 467, 487, 562, 596
BseYI CCCAGC 1 cut(s) 385
Bsh1236I CGCG 1 cut(s) 944
BshFI GGCC 2 cut(s) 116, 561
BsiHKAI GWGCWC 3 cut(s) 420, 789, 864
BsiHKCI CYCGRG 1 cut(s) 283
BsiSI CCGG 2 cut(s) 117, 284
BslFI GGGAC 3 cut(s) 265, 368, 402
BslI CCNNNNNNNGG 3 cut(s) 21, 568, 703
BsmAI GTCTC 2 cut(s) 123, 974
BsmBI CGTCTC 1 cut(s) 123
BsmFI GGGAC 3 cut(s) 265, 368, 402
BsmI GAATGC 2 cut(s) 578, 850
BsnI GGCC 2 cut(s) 116, 561
Bso31I GGTCTC 1 cut(s) 974
BsoBI CYCGRG 1 cut(s) 283
Bsp1286I GDGCHC 3 cut(s) 420, 789, 864
Bsp143I GATC 7 cut(s) 79, 216, 487, 707, 739, 937, 1060
Bsp1720I GCTNAGC 1 cut(s) 144
BspACI CCGC 5 cut(s) 36, 85, 88, 314, 697
BspANI GGCC 2 cut(s) 116, 561
BspCNI CTCAG 2 cut(s) 24, 136
BspFNI CGCG 1 cut(s) 944
BspHI TCATGA 1 cut(s) 1063
BspLI GGNNCC 1 cut(s) 709
BspMAI CTGCAG 2 cut(s) 485, 505
BspPI GGATC 2 cut(s) 702, 715
BspTNI GGTCTC 1 cut(s) 974
BsrBI CCGCTC 1 cut(s) 36
BsrDI GCAATG 1 cut(s) 138
BsrI ACTGG 2 cut(s) 562, 647
BssECI CCNNGG 3 cut(s) 282, 283, 425
BssMI GATC 7 cut(s) 79, 216, 487, 707, 739, 937, 1060
BssT1I CCWWGG 1 cut(s) 425
Bst4CI ACNGT 2 cut(s) 276, 415
BstAPI GCANNNNNTGC 1 cut(s) 868
BstC8I GCNNGC 1 cut(s) 685
BstDEI CTNAG 3 cut(s) 32, 144, 201
BstF5I GGATG 5 cut(s) 406, 672, 970, 982, 1042
BstFNI CGCG 1 cut(s) 944
BstHHI GCGC 1 cut(s) 946
BstKTI GATC 7 cut(s) 82, 219, 490, 710, 742, 940, 1063
BstMAI GTCTC 2 cut(s) 123, 974
BstMBI GATC 7 cut(s) 79, 216, 487, 707, 739, 937, 1060
BstMWI GCNNNNNNNGC 8 cut(s) 140, 500, 581, 584, 593, 693, 868, 1104
BstSCI CCNGG 2 cut(s) 282, 283
BstSFI CTRYAG 4 cut(s) 411, 481, 501, 636
BstSLI GKGCMC 2 cut(s) 420, 864
BstUI CGCG 1 cut(s) 944
BstV1I GCAGC 6 cut(s) 18, 272, 467, 487, 562, 596
BstX2I RGATCY 2 cut(s) 707, 739
BstXI CCANNNNNNTGG 1 cut(s) 797
BstYI RGATCY 2 cut(s) 707, 739
BsuRI GGCC 2 cut(s) 116, 561
BtsCI GGATG 5 cut(s) 406, 672, 970, 982, 1042
BtsI GCAGTG 1 cut(s) 387
BtsIMutI CAGTG 4 cut(s) 387, 420, 555, 654
Cac8I GCNNGC 1 cut(s) 685
CciI TCATGA 1 cut(s) 1063
CfoI GCGC 1 cut(s) 946
Cfr13I GGNCC 2 cut(s) 114, 1027
Cfr9I CCCGGG 1 cut(s) 283
CseI GACGC 1 cut(s) 140
Csp6I GTAC 1 cut(s) 64
CviAII CATG 5 cut(s) 392, 568, 791, 884, 1064
CviQI GTAC 1 cut(s) 64
DdeI CTNAG 3 cut(s) 32, 144, 201
DpnI GATC 7 cut(s) 81, 218, 489, 709, 741, 939, 1062
DpnII GATC 7 cut(s) 79, 216, 487, 707, 739, 937, 1060
EaeI YGGCCR 1 cut(s) 559
EciI GGCGGA 1 cut(s) 74
Eco130I CCWWGG 1 cut(s) 425
Eco31I GGTCTC 1 cut(s) 974
Eco47I GGWCC 1 cut(s) 1027
Eco88I CYCGRG 1 cut(s) 283
EcoT14I CCWWGG 1 cut(s) 425
ErhI CCWWGG 1 cut(s) 425
Esp3I CGTCTC 1 cut(s) 123
FaeI CATG 5 cut(s) 395, 571, 794, 887, 1067
FalI AAGNNNNNCTT 2 cut(s) 645, 677
FaqI GGGAC 3 cut(s) 265, 368, 402
FatI CATG 5 cut(s) 391, 567, 790, 883, 1063
Fnu4HI GCNGC 9 cut(s) 7, 37, 88, 261, 481, 501, 576, 585, 697
FokI GGATG 5 cut(s) 393, 659, 977, 989, 1049
Fsp4HI GCNGC 9 cut(s) 7, 37, 88, 261, 481, 501, 576, 585, 697
FspBI CTAG 4 cut(s) 123, 477, 734, 947
GlaI GCGC 1 cut(s) 945
GluI GCNGC 9 cut(s) 7, 37, 88, 261, 481, 501, 576, 585, 697
GsaI CCCAGC 1 cut(s) 389
HaeIII GGCC 2 cut(s) 116, 561
HapII CCGG 2 cut(s) 117, 284
HgaI GACGC 1 cut(s) 140
HhaI GCGC 1 cut(s) 946
Hin1II CATG 5 cut(s) 395, 571, 794, 887, 1067
Hin6I GCGC 1 cut(s) 944
HinP1I GCGC 1 cut(s) 944
HincII GTYRAC 1 cut(s) 451
HindII GTYRAC 1 cut(s) 451
HindIII AAGCTT 2 cut(s) 398, 1096
HinfI GANTC 2 cut(s) 223, 618
HpaII CCGG 2 cut(s) 117, 284
HphI GGTGA 5 cut(s) 119, 253, 328, 822, 1088
Hpy166II GTNNAC 6 cut(s) 418, 451, 610, 862, 952, 1005
Hpy188III TCNNGA 6 cut(s) 71, 220, 726, 734, 935, 1064
Hpy8I GTNNAC 6 cut(s) 418, 451, 610, 862, 952, 1005
Hpy99I CGWCG 1 cut(s) 280
HpyAV CCTTC 5 cut(s) 37, 323, 431, 464, 1076
HpyCH4III ACNGT 2 cut(s) 276, 415
HpyCH4IV ACGT 3 cut(s) 309, 357, 858
HpyF10VI GCNNNNNNNGC 8 cut(s) 140, 500, 581, 584, 593, 693, 868, 1104
HpyF3I CTNAG 3 cut(s) 32, 144, 201
HpySE526I ACGT 3 cut(s) 309, 357, 858
Hsp92II CATG 5 cut(s) 395, 571, 794, 887, 1067
HspAI GCGC 1 cut(s) 944
Kzo9I GATC 7 cut(s) 79, 216, 487, 707, 739, 937, 1060
LmnI GCTCC 2 cut(s) 119, 377
Lsp1109I GCAGC 6 cut(s) 18, 272, 467, 487, 562, 596
MaeI CTAG 4 cut(s) 123, 477, 734, 947
MaeII ACGT 3 cut(s) 309, 357, 858
MaeIII GTNAC 4 cut(s) 305, 316, 547, 664
MalI GATC 7 cut(s) 81, 218, 489, 709, 741, 939, 1062
MbiI CCGCTC 1 cut(s) 36
MboI GATC 7 cut(s) 79, 216, 487, 707, 739, 937, 1060
MboII GAAGA 4 cut(s) 734, 749, 853, 1024
MflI RGATCY 2 cut(s) 707, 739
MhlI GDGCHC 3 cut(s) 420, 789, 864
MlsI TGGCCA 1 cut(s) 561
MluCI AATT 4 cut(s) 720, 874, 1091, 1110
MluNI TGGCCA 1 cut(s) 561
Mox20I TGGCCA 1 cut(s) 561
MroXI GAANNNNTTC 1 cut(s) 741
MscI TGGCCA 1 cut(s) 561
MseI TTAA 4 cut(s) 918, 930, 1094, 1114
MslI CAYNNNNRTG 3 cut(s) 105, 792, 1068
Msp20I TGGCCA 1 cut(s) 561
MspI CCGG 2 cut(s) 117, 284
MspR9I CCNGG 2 cut(s) 284, 285
Mva1269I GAATGC 2 cut(s) 578, 850
MvnI CGCG 1 cut(s) 944
MwoI GCNNNNNNNGC 8 cut(s) 140, 500, 581, 584, 593, 693, 868, 1104
NciI CCSGG 2 cut(s) 284, 285
NdeII GATC 7 cut(s) 79, 216, 487, 707, 739, 937, 1060
NlaIII CATG 5 cut(s) 395, 571, 794, 887, 1067
NlaIV GGNNCC 1 cut(s) 709
NmuCI GTSAC 2 cut(s) 316, 664
OliI CACNNNNGTG 1 cut(s) 105
PagI TCATGA 1 cut(s) 1063
PctI GAATGC 2 cut(s) 578, 850
PdmI GAANNNNTTC 1 cut(s) 741
PfeI GAWTC 2 cut(s) 223, 618
PflFI GACNNNGTC 1 cut(s) 134
PflMI CCANNNNNTGG 2 cut(s) 21, 568
PkrI GCNGC 9 cut(s) 8, 38, 89, 262, 482, 502, 577, 586, 698
PspFI CCCAGC 1 cut(s) 385
PspN4I GGNNCC 1 cut(s) 709
PspPI GGNCC 2 cut(s) 114, 1027
PstI CTGCAG 2 cut(s) 485, 505
PsuI RGATCY 2 cut(s) 707, 739
PsyI GACNNNGTC 1 cut(s) 134
RsaI GTAC 1 cut(s) 65
RsaNI GTAC 1 cut(s) 64
RseI CAYNNNNRTG 3 cut(s) 105, 792, 1068
SaqAI TTAA 4 cut(s) 918, 930, 1094, 1114
SatI GCNGC 9 cut(s) 7, 37, 88, 261, 481, 501, 576, 585, 697
Sau3AI GATC 7 cut(s) 79, 216, 487, 707, 739, 937, 1060
Sau96I GGNCC 2 cut(s) 114, 1027
ScrFI CCNGG 2 cut(s) 284, 285
SduI GDGCHC 3 cut(s) 420, 789, 864
SfcI CTRYAG 4 cut(s) 411, 481, 501, 636
SinI GGWCC 1 cut(s) 1027
SmaI CCCGGG 1 cut(s) 285
SmiMI CAYNNNNRTG 3 cut(s) 105, 792, 1068
SmlI CTYRAG 2 cut(s) 495, 971
SmoI CTYRAG 2 cut(s) 495, 971
Sse9I AATT 4 cut(s) 720, 874, 1091, 1110
SsiI CCGC 5 cut(s) 36, 85, 88, 314, 697
SspMI CTAG 4 cut(s) 123, 477, 734, 947
StyD4I CCNGG 2 cut(s) 282, 283
StyI CCWWGG 1 cut(s) 425
TaaI ACNGT 2 cut(s) 276, 415
TaiI ACGT 3 cut(s) 312, 360, 861
TaqI TCGA 2 cut(s) 816, 924
TasI AATT 4 cut(s) 720, 874, 1091, 1110
TauI GCSGC 3 cut(s) 39, 90, 699
TfiI GAWTC 2 cut(s) 223, 618
Tru1I TTAA 4 cut(s) 918, 930, 1094, 1114
Tru9I TTAA 4 cut(s) 918, 930, 1094, 1114
TscAI CASTG 4 cut(s) 387, 420, 562, 654
TseFI GTSAC 2 cut(s) 316, 664
TseI GCWGC 6 cut(s) 6, 260, 480, 500, 575, 584
Tsp45I GTSAC 2 cut(s) 316, 664
TspDTI ATGAA 3 cut(s) 342, 993, 1095
TspMI CCCGGG 1 cut(s) 283
TspRI CASTG 4 cut(s) 387, 420, 562, 654
Tth111I GACNNNGTC 1 cut(s) 134
Van91I CCANNNNNTGG 2 cut(s) 21, 568
VneI GTGCAC 2 cut(s) 416, 860
VpaK11BI GGWCC 1 cut(s) 1027
XapI RAATTY 1 cut(s) 1110
XbaI TCTAGA 1 cut(s) 733
XcmI CCANNNNNNNNNTGG 1 cut(s) 1037
XmaI CCCGGG 1 cut(s) 283
XmnI GAANNNNTTC 1 cut(s) 741
XspI CTAG 4 cut(s) 123, 477, 734, 947
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.