RchiOBHm_Chr2g0128951

Pentatricopeptide repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
2
OG0010257
Physical Location & Seq
Forward (+)
44425638 .. 44433266
7629 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 2163 bp
ATGAGCTTCCATGGAGAATTCCTCAAACATCAAAAACGTCTTCTGCAACTACTGCATGCATGCTCAAGGGTACGATCCCTCAGGGCAACAAAAGCACTCCATGCTCTCAACATCACAATGGGTCTGTCTCCAAAGCAGCCCATCTTTGTTTACAACAATATCATGTCACAATATTCCTCACTCGGGAAGTTATTAGTTGCACGTAAATTGTTTGATAAGATGCCTCTTAGAAACGTTGTGTCATATAATATCATGATTAGTGCTTATTGTACTTCTGGGTATGTTGGGGAAGCTTGGAAAATGTTTTATGAGATGAGGGTTTCTGGGTTTCAGCCAACTCAGTATGCAGTTAGCAGGTTATTGTCATGTGGGTCATTGGATATTCATCATGGGGTCCTGTTGCATTCTTTGGTAATAAAGAACGGACTGTTTGATGGTGATGCTTTTGTGGGGACTTCCTTGTTGGGTTTTTATGGAAGGCATGGGCTGTTAGAGGAAGCGGTTTGGACATTTCAGGATATGCCTTGTAGGAGCTTGGTGACATGGAATTCCATGATATCTTTGCTTGGGAATCATGGGTTTTTTGAAAATTGTGTGTTTTTGTTTCGGGAGCTGGTGAGGAGGGATGTTAATTTGTCTGAAGGTTCTTTTATGGGTGTTCTTTCTGCATTTTCATGCCCGCAAGACTTGGAATTTGGGGAACAGTTGCATGCTTTTGTTATAAAGAATGGGTTTACTTGTGAAATTTTGGTGATGAATTCTCTAATAAGTATGTATGTGAAATGCAGTGCCATATGCTCGGCAGAGAAAATTTTTAAAGAAGTACAATTTAAAGATGTTGTATCATGGAATACTATCATTGGTGCGACAGCAAAAATTGGGGGACCCCAAAAAGCAGTAGAACTCTTCTCTCAAATGTCCATGGCTGGAGTGTTGCCTACCGAGATCACATTTGTTAGTCTAATAAACTGTTGTACCAGTTTGGAGGTTCCAATCTCTGGAGAGTCAGTTCATGCTAAGATAATCCAGCATGCTTACGAATCTAATGTCTTAGTAGCTGCCCATCGCTGTTTTTATGAGATATGTGCGAAAAATGTGGTCTCTTGGAATGCTTTGATTTTGGGTTACTCAAATAGTTCCTCACCTACATGTATACTCTTAGTGCAAGAAATGCTTCATTTGGGTTACCGACCTAACGAGTTTTCATTTTCTGCTGTTCTTAAGTCATCATTGGCATTAGAGCTCCAACAACTTCATTGCTTAGTTGTAAGAATGGGCTTTGAGCAAAATTATTATGTTCTGAGCTCTCTTATAATGTCGTATACCAAAAGTGGTTTAATATCTGATGCTCTGGTTTTTGTCACAGCTTCTGATGCTCCCTCTAATGCCATTGCTGGAATTTACAACAGAACTGGGCAATATAATGAAACTCTAAGATTCCTTTGTCCACTTGAAGAACTTGATATTTTTTCCTGGAACATTGTGATTTCAGCTCTTGGCCGCATCGGTTATTACAAAGAGGTATTTGAGCTTTTCCAACAGATGCAGTTCAATCAGGTCTGGCCAGACAATTATACATTTGTTTGTCTTCTAAGTGTCTGTGCTAAACTTTGTAATCTTGCTTTGGGAAGTTCTCTTCATGCTTATATCATTAAGACTGATTTCAGTTCTTGTGACACATTTGCATGCAACGTTTTACTTGACATGTATGGGAAATGTGGTAGCATTGGAAACTCAGTGAAAATCTTTGACAAAATGAGATACAAAAACTTAATTACCTGGACAGCTTTGATTTCTGCTCTTGGAGTTAATGGTTATGTTCACGAGGCATTAGAAAGATTTAGAGAAATGATCTTCTTGGGCTTTAAGCCTGATGGAGTAGCTTTTACTGCTGTTCTTACAGCTTGCAGGCATGGTGGGTTAGTTAGGGATGGATTGGAGTTATTTGGGCAAATGATAAATAATTATGGGGTTGAACCAAAAATGGATCATTACCAGTGTGTGGTGGACTTACTGGTTAAAGGTGGTCATGTTACAGAAGCAGAGAAACTAATTTCCAGCATGGCCTTCCCACCAAATATGATTATATGGCGTTGTTTCCTTGAAGGCTGCAAGAGTCATGGAAATGCAGTGGACGTGGACCAGGCAGTAGCACATGCTTAA

Protein Analysis

720

Amino Acids

80.37

Weight (kDa)

7.04

Isoelectric Point (pI)

35.77

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PPR_2 PF13041 77 - 121 5.7e-12 PPR repeat family
PPR_1 PF12854 78 - 106 2e-08 PPR repeat
PPR PF01535 80 - 110 1.9e-08 PPR repeat
PPR_2 PF13041 278 - 325 3.5e-11 PPR repeat family
PPR_2 PF13041 488 - 536 1.3e-10 PPR repeat family
PPR_2 PF13041 590 - 636 8.3e-08 PPR repeat family
PPR PF01535 592 - 621 6.5e-06 PPR repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 722, 1313
Acc36I ACCTGC 1 cut(s) 345
AccB7I CCANNNNNTGG 2 cut(s) 998, 2002
AccI GTMKAC 2 cut(s) 1153, 1322
AciI CCGC 3 cut(s) 500, 680, 1501
AclI AACGTT 2 cut(s) 234, 1692
AclWI GGATC 2 cut(s) 69, 1995
AcoI YGGCCR 2 cut(s) 1498, 1562
AcsI RAATTY 7 cut(s) 17, 547, 692, 744, 757, 810, 1398
AcuI CTGAAG 1 cut(s) 660
AfaI GTAC 4 cut(s) 72, 271, 825, 976
AfiI CCNNNNNNNGG 3 cut(s) 183, 998, 2002
AflII CTTAAG 1 cut(s) 1220
AflIII ACRYGT 2 cut(s) 1148, 1704
AgsI TTSAA 5 cut(s) 587, 1454, 1552, 1976, 2105
AjiI CACGTC 1 cut(s) 2137
AjnI CCWGG 3 cut(s) 1472, 1778, 2142
AloI GAACNNNNNNTCC 2 cut(s) 993, 1025
Alw21I GWGCWC 2 cut(s) 1245, 1307
Alw26I GTCTC 2 cut(s) 132, 1105
AlwI GGATC 2 cut(s) 69, 1995
AlwNI CAGNNNCTG 1 cut(s) 1370
Ama87I CYCGRG 1 cut(s) 182
AoxI GGCC 3 cut(s) 1498, 1562, 2064
ApeKI GCWGC 3 cut(s) 136, 1058, 2109
ApoI RAATTY 7 cut(s) 17, 547, 692, 744, 757, 810, 1398
ArsI GACNNNNNNTTYG 4 cut(s) 677, 709, 1032, 1064
Asp700I GAANNNNTTC 1 cut(s) 1173
AspS9I GGNCC 3 cut(s) 394, 884, 2140
AsuHPI GGTGA 5 cut(s) 449, 550, 628, 763, 1134
AvaI CYCGRG 1 cut(s) 182
AvaII GGWCC 3 cut(s) 394, 884, 2140
AxyI CCTNAGG 1 cut(s) 80
BaeI ACNNNNGTAYC 2 cut(s) 958, 991
BalI TGGCCA 1 cut(s) 1564
BanII GRGCYC 2 cut(s) 1245, 1307
BauI CACGAG 1 cut(s) 1823
BbsI GAAGAC 2 cut(s) 32, 1580
Bbv12I GWGCWC 2 cut(s) 1245, 1307
BbvI GCAGC 3 cut(s) 148, 1045, 2096
BccI CCATC 5 cut(s) 149, 428, 1071, 1868, 1925
BciT130I CCWGG 3 cut(s) 1474, 1780, 2144
BcoDI GTCTC 2 cut(s) 132, 1105
BfrI CTTAAG 1 cut(s) 1220
BfuAI ACCTGC 1 cut(s) 345
BisI GCNGC 4 cut(s) 137, 1059, 1501, 2110
BlsI GCNGC 4 cut(s) 138, 1060, 1502, 2111
Bme1390I CCNGG 3 cut(s) 1474, 1780, 2144
Bme18I GGWCC 3 cut(s) 394, 884, 2140
BmeT110I CYCGRG 1 cut(s) 182
BmgBI CACGTC 1 cut(s) 2137
BmgT120I GGNCC 3 cut(s) 394, 884, 2140
BmiI GGNNCC 4 cut(s) 395, 885, 886, 990
BmrFI CCNGG 3 cut(s) 1474, 1780, 2144
BmrI ACTGGG 1 cut(s) 1422
BmsI GCATC 6 cut(s) 210, 430, 1336, 1363, 1512, 1533
BmuI ACTGGG 1 cut(s) 1422
BpiI GAAGAC 2 cut(s) 32, 1580
BplI GAGNNNNNCTC 1 cut(s) 38
BpmI CTGGAG 2 cut(s) 948, 1020
BpuEI CTTGAG 1 cut(s) 49
BsaAI YACGTR 1 cut(s) 203
BsaBI GATNNNNATC 1 cut(s) 384
BsaI GGTCTC 1 cut(s) 1105
BsaJI CCNNGG 2 cut(s) 10, 921
BsaXI ACNNNNNCTCC 2 cut(s) 993, 1023
Bsc4I CCNNNNNNNGG 3 cut(s) 183, 998, 2002
Bse1I ACTGG 4 cut(s) 978, 1417, 1996, 2019
Bse21I CCTNAGG 1 cut(s) 80
Bse3DI GCAATG 2 cut(s) 1255, 1389
Bse8I GATNNNNATC 1 cut(s) 384
BseBI CCWGG 3 cut(s) 1474, 1780, 2144
BseDI CCNNGG 2 cut(s) 10, 921
BseGI GGATG 2 cut(s) 631, 1936
BseJI GATNNNNATC 1 cut(s) 384
BseLI CCNNNNNNNGG 3 cut(s) 183, 998, 2002
BseMI GCAATG 2 cut(s) 1255, 1389
BseMII CTCAG 4 cut(s) 94, 353, 1292, 1749
BseNI ACTGG 4 cut(s) 978, 1417, 1996, 2019
BseRI GAGGAG 1 cut(s) 634
BseXI GCAGC 3 cut(s) 148, 1045, 2096
BshFI GGCC 3 cut(s) 1500, 1564, 2066
BsiHKAI GWGCWC 2 cut(s) 1245, 1307
BsiHKCI CYCGRG 1 cut(s) 182
BslFI GGGAC 2 cut(s) 466, 897
BslI CCNNNNNNNGG 3 cut(s) 183, 998, 2002
BsmAI GTCTC 2 cut(s) 132, 1105
BsmFI GGGAC 2 cut(s) 466, 897
BsmI GAATGC 2 cut(s) 403, 1114
BsnI GGCC 3 cut(s) 1500, 1564, 2066
Bso31I GGTCTC 1 cut(s) 1105
BsoBI CYCGRG 1 cut(s) 182
Bsp1286I GDGCHC 2 cut(s) 1245, 1307
Bsp143I GATC 4 cut(s) 74, 945, 1851, 1987
Bsp19I CCATGG 2 cut(s) 10, 921
BspACI CCGC 3 cut(s) 500, 680, 1501
BspANI GGCC 3 cut(s) 1500, 1564, 2066
BspCNI CTCAG 4 cut(s) 93, 352, 1293, 1748
BspHI TCATGA 1 cut(s) 252
BspLI GGNNCC 4 cut(s) 395, 885, 886, 990
BspMI ACCTGC 1 cut(s) 345
BspPI GGATC 2 cut(s) 69, 1995
BspTI CTTAAG 1 cut(s) 1220
BspTNI GGTCTC 1 cut(s) 1105
BsrDI GCAATG 2 cut(s) 1255, 1389
BsrI ACTGG 4 cut(s) 978, 1417, 1996, 2019
BssECI CCNNGG 2 cut(s) 10, 921
BssMI GATC 4 cut(s) 74, 945, 1851, 1987
BssNAI GTATAC 2 cut(s) 1154, 1323
BssSI CACGAG 1 cut(s) 1823
BssT1I CCWWGG 2 cut(s) 10, 921
Bst1107I GTATAC 2 cut(s) 1154, 1323
Bst2BI CACGAG 1 cut(s) 1823
Bst2UI CCWGG 3 cut(s) 1474, 1780, 2144
Bst4CI ACNGT 3 cut(s) 429, 705, 971
Bst6I CTCTTC 2 cut(s) 911, 1641
BstAFI CTTAAG 1 cut(s) 1220
BstAPI GCANNNNNTGC 3 cut(s) 52, 101, 1171
BstBAI YACGTR 1 cut(s) 203
BstC8I GCNNGC 8 cut(s) 57, 61, 680, 711, 1032, 1687, 1906, 1910
BstDSI CCRYGG 2 cut(s) 10, 921
BstEII GGTNACC 1 cut(s) 1184
BstF5I GGATG 2 cut(s) 631, 1936
BstKTI GATC 4 cut(s) 77, 948, 1854, 1990
BstMAI GTCTC 2 cut(s) 132, 1105
BstMBI GATC 4 cut(s) 74, 945, 1851, 1987
BstMWI GCNNNNNNNGC 6 cut(s) 52, 92, 101, 1171, 1373, 1889
BstNI CCWGG 3 cut(s) 1474, 1780, 2144
BstNSI RCATGY 8 cut(s) 59, 63, 713, 1034, 1152, 1689, 1708, 2159
BstPI GGTNACC 1 cut(s) 1184
BstSCI CCNGG 3 cut(s) 1472, 1778, 2142
BstV1I GCAGC 3 cut(s) 148, 1045, 2096
BstV2I GAAGAC 2 cut(s) 32, 1580
BstZ17I GTATAC 2 cut(s) 1154, 1323
Bsu36I CCTNAGG 1 cut(s) 80
BsuRI GGCC 3 cut(s) 1500, 1564, 2066
BtgI CCRYGG 2 cut(s) 10, 921
BtgZI GCGATG 1 cut(s) 1049
BtrI CACGTC 1 cut(s) 2137
BtsCI GGATG 2 cut(s) 631, 1936
BtsI GCAGTG 2 cut(s) 793, 2136
BtsIMutI CAGTG 4 cut(s) 793, 1743, 2003, 2136
BveI ACCTGC 1 cut(s) 345
Cac8I GCNNGC 8 cut(s) 57, 61, 680, 711, 1032, 1687, 1906, 1910
CaiI CAGNNNCTG 1 cut(s) 1370
CciI TCATGA 1 cut(s) 252
Cfr13I GGNCC 3 cut(s) 394, 884, 2140
Csp6I GTAC 4 cut(s) 71, 270, 824, 975
CviQI GTAC 4 cut(s) 71, 270, 824, 975
DpnI GATC 4 cut(s) 76, 947, 1853, 1989
DpnII GATC 4 cut(s) 74, 945, 1851, 1987
DraI TTTAAA 2 cut(s) 817, 832
EaeI YGGCCR 2 cut(s) 1498, 1562
Eam1104I CTCTTC 2 cut(s) 911, 1641
EarI CTCTTC 2 cut(s) 911, 1641
Ecl136II GAGCTC 2 cut(s) 1243, 1305
Eco130I CCWWGG 2 cut(s) 10, 921
Eco24I GRGCYC 2 cut(s) 1245, 1307
Eco31I GGTCTC 1 cut(s) 1105
Eco32I GATATC 1 cut(s) 558
Eco47I GGWCC 3 cut(s) 394, 884, 2140
Eco53kI GAGCTC 2 cut(s) 1243, 1305
Eco57I CTGAAG 1 cut(s) 660
Eco81I CCTNAGG 1 cut(s) 80
Eco88I CYCGRG 1 cut(s) 182
Eco91I GGTNACC 1 cut(s) 1184
EcoICRI GAGCTC 2 cut(s) 1243, 1305
EcoO109I RGGNCCY 2 cut(s) 394, 884
EcoO65I GGTNACC 1 cut(s) 1184
EcoRI GAATTC 3 cut(s) 17, 547, 757
EcoRII CCWGG 3 cut(s) 1472, 1778, 2142
EcoRV GATATC 1 cut(s) 558
EcoT14I CCWWGG 2 cut(s) 10, 921
EcoT22I ATGCAT 1 cut(s) 61
EcoT38I GRGCYC 2 cut(s) 1245, 1307
ErhI CCWWGG 2 cut(s) 10, 921
FalI AAGNNNNNCTT 2 cut(s) 1158, 1190
FaqI GGGAC 2 cut(s) 466, 897
FauI CCCGC 1 cut(s) 687
FauNDI CATATG 1 cut(s) 794
FblI GTMKAC 2 cut(s) 1153, 1322
Fnu4HI GCNGC 4 cut(s) 137, 1059, 1501, 2110
FokI GGATG 2 cut(s) 638, 1943
FriOI GRGCYC 2 cut(s) 1245, 1307
Fsp4HI GCNGC 4 cut(s) 137, 1059, 1501, 2110
GluI GCNGC 4 cut(s) 137, 1059, 1501, 2110
GsuI CTGGAG 2 cut(s) 948, 1020
HaeIII GGCC 3 cut(s) 1500, 1564, 2066
HindIII AAGCTT 1 cut(s) 291
HinfI GANTC 5 cut(s) 571, 1004, 1040, 1437, 2116
HphI GGTGA 5 cut(s) 449, 550, 628, 763, 1134
Hpy166II GTNNAC 9 cut(s) 151, 735, 1154, 1323, 1448, 1822, 2008, 2134, 2140
Hpy188I TCNGA 4 cut(s) 640, 1302, 1345, 1372
Hpy188III TCNNGA 6 cut(s) 184, 253, 515, 608, 999, 1823
Hpy8I GTNNAC 9 cut(s) 151, 735, 1154, 1323, 1448, 1822, 2008, 2134, 2140
HpyAV CCTTC 4 cut(s) 471, 635, 2077, 2099
HpyCH4III ACNGT 3 cut(s) 429, 705, 971
HpyCH4IV ACGT 5 cut(s) 37, 202, 234, 1692, 2136
HpyF10VI GCNNNNNNNGC 6 cut(s) 52, 92, 101, 1171, 1373, 1889
HpySE526I ACGT 5 cut(s) 37, 202, 234, 1692, 2136
KflI GGGWCCC 1 cut(s) 884
Kzo9I GATC 4 cut(s) 74, 945, 1851, 1987
LmnI GCTCC 4 cut(s) 531, 610, 1248, 1381
Lsp1109I GCAGC 3 cut(s) 148, 1045, 2096
LweI GCATC 6 cut(s) 210, 430, 1336, 1363, 1512, 1533
MaeII ACGT 5 cut(s) 37, 202, 234, 1692, 2136
MaeIII GTNAC 7 cut(s) 165, 538, 1124, 1184, 1360, 1673, 2032
MalI GATC 4 cut(s) 76, 947, 1853, 1989
MboI GATC 4 cut(s) 74, 945, 1851, 1987
MboII GAAGA 6 cut(s) 32, 898, 1466, 1580, 1628, 1846
MhlI GDGCHC 2 cut(s) 1245, 1307
MlsI TGGCCA 1 cut(s) 1564
MluNI TGGCCA 1 cut(s) 1564
MlyI GAGTC 2 cut(s) 1013, 2125
MmeI TCCRAC 2 cut(s) 1270, 1561
Mox20I TGGCCA 1 cut(s) 1564
Mph1103I ATGCAT 1 cut(s) 61
MroXI GAANNNNTTC 1 cut(s) 1173
MscI TGGCCA 1 cut(s) 1564
MslI CAYNNNNRTG 5 cut(s) 116, 673, 1147, 1684, 2124
Msp20I TGGCCA 1 cut(s) 1564
MspCI CTTAAG 1 cut(s) 1220
MspR9I CCNGG 3 cut(s) 1474, 1780, 2144
Mva1269I GAATGC 2 cut(s) 403, 1114
MvaI CCWGG 3 cut(s) 1474, 1780, 2144
MwoI GCNNNNNNNGC 6 cut(s) 52, 92, 101, 1171, 1373, 1889
NcoI CCATGG 2 cut(s) 10, 921
NdeI CATATG 1 cut(s) 794
NdeII GATC 4 cut(s) 74, 945, 1851, 1987
NlaIV GGNNCC 4 cut(s) 395, 885, 886, 990
NmeAIII GCCGAG 1 cut(s) 779
NmuCI GTSAC 4 cut(s) 165, 538, 1360, 1673
NsiI ATGCAT 1 cut(s) 61
NspI RCATGY 8 cut(s) 59, 63, 713, 1034, 1152, 1689, 1708, 2159
PaeI GCATGC 5 cut(s) 59, 63, 713, 1034, 1689
PagI TCATGA 1 cut(s) 252
PciI ACATGT 2 cut(s) 1148, 1704
PctI GAATGC 2 cut(s) 403, 1114
PdmI GAANNNNTTC 1 cut(s) 1173
PfeI GAWTC 3 cut(s) 571, 1040, 1437
PflMI CCANNNNNTGG 2 cut(s) 998, 2002
PfoI TCCNGGA 1 cut(s) 1472
PkrI GCNGC 4 cut(s) 138, 1060, 1502, 2111
PleI GAGTC 2 cut(s) 1012, 2124
PpsI GAGTC 2 cut(s) 1012, 2124
Ppu21I YACGTR 1 cut(s) 203
PpuMI RGGWCCY 2 cut(s) 394, 884
PscI ACATGT 2 cut(s) 1148, 1704
PsiI TTATAA 2 cut(s) 722, 1313
Psp124BI GAGCTC 2 cut(s) 1245, 1307
Psp1406I AACGTT 2 cut(s) 234, 1692
Psp5II RGGWCCY 2 cut(s) 394, 884
Psp6I CCWGG 3 cut(s) 1472, 1778, 2142
PspEI GGTNACC 1 cut(s) 1184
PspGI CCWGG 3 cut(s) 1472, 1778, 2142
PspN4I GGNNCC 4 cut(s) 395, 885, 886, 990
PspPI GGNCC 3 cut(s) 394, 884, 2140
PspPPI RGGWCCY 2 cut(s) 394, 884
PstNI CAGNNNCTG 1 cut(s) 1370
RsaI GTAC 4 cut(s) 72, 271, 825, 976
RsaNI GTAC 4 cut(s) 71, 270, 824, 975
RseI CAYNNNNRTG 5 cut(s) 116, 673, 1147, 1684, 2124
SacI GAGCTC 2 cut(s) 1245, 1307
SatI GCNGC 4 cut(s) 137, 1059, 1501, 2110
Sau3AI GATC 4 cut(s) 74, 945, 1851, 1987
Sau96I GGNCC 3 cut(s) 394, 884, 2140
SchI GAGTC 2 cut(s) 1013, 2125
ScrFI CCNGG 3 cut(s) 1474, 1780, 2144
SduI GDGCHC 2 cut(s) 1245, 1307
SfaNI GCATC 6 cut(s) 210, 430, 1336, 1363, 1512, 1533
SinI GGWCC 3 cut(s) 394, 884, 2140
SmiMI CAYNNNNRTG 5 cut(s) 116, 673, 1147, 1684, 2124
SmlI CTYRAG 2 cut(s) 64, 1220
SmoI CTYRAG 2 cut(s) 64, 1220
SphI GCATGC 5 cut(s) 59, 63, 713, 1034, 1689
SsiI CCGC 3 cut(s) 500, 680, 1501
SspI AATATT 1 cut(s) 173
SstI GAGCTC 2 cut(s) 1245, 1307
StyD4I CCNGG 3 cut(s) 1472, 1778, 2142
StyI CCWWGG 2 cut(s) 10, 921
TaaI ACNGT 3 cut(s) 429, 705, 971
TaiI ACGT 5 cut(s) 40, 205, 237, 1695, 2139
TatI WGTACW 2 cut(s) 269, 823
TauI GCSGC 1 cut(s) 1503
TfiI GAWTC 3 cut(s) 571, 1040, 1437
TscAI CASTG 4 cut(s) 793, 1743, 2003, 2136
TseFI GTSAC 4 cut(s) 165, 538, 1360, 1673
TseI GCWGC 3 cut(s) 136, 1058, 2109
Tsp45I GTSAC 4 cut(s) 165, 538, 1360, 1673
TspDTI ATGAA 9 cut(s) 374, 663, 770, 1001, 1166, 1194, 1244, 1440, 1628
TspGWI ACGGA 1 cut(s) 438
TspRI CASTG 4 cut(s) 793, 1743, 2003, 2136
Van91I CCANNNNNTGG 2 cut(s) 998, 2002
Vha464I CTTAAG 1 cut(s) 1220
VpaK11BI GGWCC 3 cut(s) 394, 884, 2140
XapI RAATTY 7 cut(s) 17, 547, 692, 744, 757, 810, 1398
XceI RCATGY 8 cut(s) 59, 63, 713, 1034, 1152, 1689, 1708, 2159
XmiI GTMKAC 2 cut(s) 1153, 1322
XmnI GAANNNNTTC 1 cut(s) 1173
Zsp2I ATGCAT 1 cut(s) 61
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.