Rroxscaffold_2G00115030

Pentatricopeptide repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000002
OG0010257
Physical Location & Seq
Reverse (-)
41178375 .. 41185451
7077 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_2G00115030.1

Sequence Viewer

Length: 2223 bp
ATGAGCTTCCATGGAGAATTCCTCAAACATCAAAAACGTCTTCAGCAACTACTGCATGCATGCTCAAGGGTACGATCCCTCAGGGCAACAAAAGCACTCCATGCTCTCAACATCACAATGGGTCTGTCTCCAAAGCAGCCCATCTTTGTTTACAACAATATCATGTCACAATATTCCTCACTCGGGAAGTTATTAATTGCACGTAAATTGTTTGATAGGATGCCTCTTAGAAATGTTGTGTCATATAATATCATGATTACTGCTTATACTACATCTGGGTATGTTGGGGAAGCGTGGAAAATGTTTTATGAGATGAGGGTTTCTGGGTTTGAGCCAACTCAGTATGCAGTTAGCAGGTTATTGTCATGCGGGTCAGTGGATATTTATCATGGGGTCCTGTTGCATTCTTTGGCAATAAAGAACGGACTGTTTGATGGTGATGCTTTTGTGGGGACTTCCTTGTTGGGTTTTTATGGAAGGCATTGGCTGTTAGAGGAAGCGGTTTGGACATTTCAGGATATGCCTTGTAGGAGCTTGGTGACATGGAATTCCATGATATCTTTGCTTGGGAATCGTGGGTTTTTTGAAAATTGTGTGTTTTTGTTTCGGGAGCTGGTGAGGAGGGATGTTGATTTGTCTGAAGGTTCTTTTATGGGTGTTTTTTCTGCATTTTCATGCCCACAAGACTTGGAATTTGGGGAACAGTTGCATGCTTTTGTTATAAAGAATGGGTTTACTTGTGAAATTTTGGTGATGAATTCTCTAATAAGTATGTATGTGAAATGCAGTGCCATATGCTCGGCAGAGAAAATTTTTAAAGAAGTACAATTTAAAGATGTTGTATCATGGAATACTATCATTGGTGCAATAGCAAAAATTGGGGGACCCCAAAAAGCAGTAGAACTTTTCTCTCAAATGTCCATGGCTGGAGTGTTGCCTACTGAGATCACATTTGTTAGTCTAATAAACTGTTGTACCAGTTTGGAGGTTCCAATCTCTGGAGAGTCTGTTCATGCTAAGATAATCCAGCATGCTTACGAATCTAATGTCTTAGTAGGTAGTGCATTGGTTGATTTTTATGCTAAATGTGATAACTTGAAAGCTGCCCATCGCTGTTTTTATGAGATATGTGAGAAAAATGTGGTCTCTTGGAATGCTTTGATTTTGGGTTACTCGAATAGTTCCTCACCTACATGTATACTCTTACTGCAAGAAATGCTTCATTTGGGTTACCGACCTAACGAGTTTTCATTTTCTGCTGTTCTTAAGTCATCATTGGCATTAGAGCTCCAACAACTTCATTGCTTAGTTGTAAGAATGGGCTTGGAGCAAAATTATTATGTTCTGAGTTCTCTTATAATGTCGTATACCAAAAGTGGTTTAATATCTGATGCCCTGGTCTTTGTCACAGCTTCTGATGGTCTGCTTCCTGATGTTCCCTCTAATGCCATTGCTGGAATTTACAACAGAACTGGGCAATATAATGAAACTCTAAGATTCCTTTGTCCACTTGAAGAACTTGATATTGTTTCCTGGAACATTGTGATTTCAGCTCTTGGCCGCATCGGTTATTACAAAGAGGTCTTTGAGCTTTTCCAACAGATGCAATTCAATCAGGTCTGGCCAGACAATTATACATTTGTTAGCCTTCTTAGTGTCTGTGCTAAAACTTGTAATCTTGCTTTGGGAAGTTCTCTTCATGCTTATATCATTAAGACTGATTTCAGTTCTTGTGACACATTTGCATGCAACGTTTTACTTGACATGTATGGGAAATGTGGTAGCATTGGAAACTCAGTGAAAATCTTTGACGAAATGAGATGCAAAAACTTAATTACCTGGACAGCTTTGATTTCTGCTCTTGGAGTTAATGGTTATGTTCACAAGGCATTAGAAAGATTTAGAGAAATGATCTTCTTGGGCTTTCAGCCTGATGGAGTAGCTTTTACTGCTGTTCTTACAGCTTGCAGGCATGGTGGGTTAGTTAGGGATGGATTGGAGTTATTTGGGCAAATGATAAATAATTATGGGGTTGAACCAAAAATGGATCATTACCAGTGTGTGGTGGACTTACTGGTTAAAGGTGGTCATGTTACAGAAGCAGAGAAACTAATTTCCAGCATGGCCTTCCCACCAAATATGATTATTTGGCGTTGTTTCCTTGAAGGCTGCAAGAGTCGTGGAAATGCAGTGGACGTGGGCCAAGCAGTAGCACAATCTTAA

Protein Analysis

740

Amino Acids

82.64

Weight (kDa)

6.65

Isoelectric Point (pI)

36.81

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PPR_2 PF13041 77 - 121 2e-10 PPR repeat family
PPR PF01535 80 - 110 1.1e-06 PPR repeat
PPR_2 PF13041 278 - 325 1.6e-11 PPR repeat family
PPR_2 PF13041 508 - 556 3.6e-11 PPR repeat family
PPR PF01535 510 - 536 6.7e-06 PPR repeat
PPR_2 PF13041 610 - 656 1.4e-07 PPR repeat family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 722, 1358
Acc36I ACCTGC 1 cut(s) 345
AccB7I CCANNNNNTGG 2 cut(s) 998, 2062
AccI GTMKAC 2 cut(s) 1198, 1367
AciI CCGC 3 cut(s) 369, 500, 1561
AclI AACGTT 1 cut(s) 1752
AclWI GGATC 2 cut(s) 69, 2055
AcoI YGGCCR 2 cut(s) 1558, 1622
AcsI RAATTY 7 cut(s) 17, 547, 692, 744, 757, 810, 1458
AcuI CTGAAG 2 cut(s) 26, 660
AfaI GTAC 3 cut(s) 72, 825, 976
AfiI CCNNNNNNNGG 3 cut(s) 183, 998, 2062
AflII CTTAAG 1 cut(s) 1265
AflIII ACRYGT 2 cut(s) 1193, 1764
AgsI TTSAA 6 cut(s) 587, 1099, 1514, 1612, 2036, 2165
AjiI CACGTC 1 cut(s) 2197
AjnI CCWGG 3 cut(s) 1395, 1532, 1838
AloI GAACNNNNNNTCC 2 cut(s) 993, 1025
Alw21I GWGCWC 1 cut(s) 1290
Alw26I GTCTC 2 cut(s) 132, 1150
AlwI GGATC 2 cut(s) 69, 2055
AlwNI CAGNNNCTG 1 cut(s) 1415
Ama87I CYCGRG 1 cut(s) 182
AoxI GGCC 4 cut(s) 1558, 1622, 2124, 2200
ApeKI GCWGC 3 cut(s) 136, 1103, 2169
ApoI RAATTY 7 cut(s) 17, 547, 692, 744, 757, 810, 1458
ArsI GACNNNNNNTTYG 4 cut(s) 677, 709, 1032, 1064
AseI ATTAAT 1 cut(s) 194
Asp700I GAANNNNTTC 1 cut(s) 1218
AspS9I GGNCC 3 cut(s) 394, 884, 2200
AsuHPI GGTGA 5 cut(s) 449, 550, 628, 763, 1179
AvaI CYCGRG 1 cut(s) 182
AvaII GGWCC 2 cut(s) 394, 884
AxyI CCTNAGG 1 cut(s) 80
BaeI ACNNNNGTAYC 2 cut(s) 958, 991
BalI TGGCCA 1 cut(s) 1624
BanII GRGCYC 1 cut(s) 1290
BbsI GAAGAC 1 cut(s) 32
Bbv12I GWGCWC 1 cut(s) 1290
BbvI GCAGC 3 cut(s) 148, 1090, 2156
BccI CCATC 6 cut(s) 149, 428, 1116, 1412, 1928, 1985
BciT130I CCWGG 3 cut(s) 1397, 1534, 1840
BcoDI GTCTC 2 cut(s) 132, 1150
BfrI CTTAAG 1 cut(s) 1265
BfuAI ACCTGC 1 cut(s) 345
BisI GCNGC 4 cut(s) 137, 1104, 1561, 2170
BlsI GCNGC 4 cut(s) 138, 1105, 1562, 2171
Bme1390I CCNGG 3 cut(s) 1397, 1534, 1840
Bme18I GGWCC 2 cut(s) 394, 884
BmeT110I CYCGRG 1 cut(s) 182
BmgBI CACGTC 1 cut(s) 2197
BmgT120I GGNCC 3 cut(s) 394, 884, 2200
BmiI GGNNCC 4 cut(s) 395, 885, 886, 990
BmrFI CCNGG 3 cut(s) 1397, 1534, 1840
BmrI ACTGGG 1 cut(s) 1482
BmsI GCATC 6 cut(s) 210, 430, 1381, 1572, 1593, 1811
BmuI ACTGGG 1 cut(s) 1482
BpiI GAAGAC 1 cut(s) 32
BplI GAGNNNNNCTC 1 cut(s) 38
BpmI CTGGAG 2 cut(s) 948, 1020
BpuEI CTTGAG 1 cut(s) 49
BsaAI YACGTR 1 cut(s) 203
BsaBI GATNNNNATC 1 cut(s) 384
BsaI GGTCTC 1 cut(s) 1150
BsaJI CCNNGG 3 cut(s) 10, 921, 1395
BsaXI ACNNNNNCTCC 2 cut(s) 993, 1023
Bsc4I CCNNNNNNNGG 3 cut(s) 183, 998, 2062
Bse1I ACTGG 4 cut(s) 978, 1477, 2056, 2079
Bse21I CCTNAGG 1 cut(s) 80
Bse3DI GCAATG 2 cut(s) 1300, 1449
Bse8I GATNNNNATC 1 cut(s) 384
BseBI CCWGG 3 cut(s) 1397, 1534, 1840
BseDI CCNNGG 3 cut(s) 10, 921, 1395
BseGI GGATG 3 cut(s) 225, 631, 1996
BseJI GATNNNNATC 1 cut(s) 384
BseLI CCNNNNNNNGG 3 cut(s) 183, 998, 2062
BseMI GCAATG 2 cut(s) 1300, 1449
BseMII CTCAG 5 cut(s) 94, 353, 933, 1337, 1809
BseNI ACTGG 4 cut(s) 978, 1477, 2056, 2079
BseRI GAGGAG 1 cut(s) 634
BseXI GCAGC 3 cut(s) 148, 1090, 2156
BshFI GGCC 4 cut(s) 1560, 1624, 2126, 2202
BsiHKAI GWGCWC 1 cut(s) 1290
BsiHKCI CYCGRG 1 cut(s) 182
BslFI GGGAC 2 cut(s) 466, 897
BslI CCNNNNNNNGG 3 cut(s) 183, 998, 2062
BsmAI GTCTC 2 cut(s) 132, 1150
BsmFI GGGAC 2 cut(s) 466, 897
BsmI GAATGC 2 cut(s) 403, 1159
BsnI GGCC 4 cut(s) 1560, 1624, 2126, 2202
Bso31I GGTCTC 1 cut(s) 1150
BsoBI CYCGRG 1 cut(s) 182
Bsp1286I GDGCHC 1 cut(s) 1290
Bsp143I GATC 4 cut(s) 74, 945, 1911, 2047
Bsp19I CCATGG 2 cut(s) 10, 921
BspACI CCGC 3 cut(s) 369, 500, 1561
BspANI GGCC 4 cut(s) 1560, 1624, 2126, 2202
BspCNI CTCAG 5 cut(s) 93, 352, 934, 1338, 1808
BspHI TCATGA 1 cut(s) 252
BspLI GGNNCC 4 cut(s) 395, 885, 886, 990
BspMI ACCTGC 1 cut(s) 345
BspPI GGATC 2 cut(s) 69, 2055
BspTI CTTAAG 1 cut(s) 1265
BspTNI GGTCTC 1 cut(s) 1150
BsrDI GCAATG 2 cut(s) 1300, 1449
BsrI ACTGG 4 cut(s) 978, 1477, 2056, 2079
BssECI CCNNGG 3 cut(s) 10, 921, 1395
BssMI GATC 4 cut(s) 74, 945, 1911, 2047
BssNAI GTATAC 2 cut(s) 1199, 1368
BssT1I CCWWGG 2 cut(s) 10, 921
Bst1107I GTATAC 2 cut(s) 1199, 1368
Bst2UI CCWGG 3 cut(s) 1397, 1534, 1840
Bst4CI ACNGT 3 cut(s) 429, 705, 971
Bst6I CTCTTC 1 cut(s) 1701
BstAFI CTTAAG 1 cut(s) 1265
BstAPI GCANNNNNTGC 3 cut(s) 52, 101, 1216
BstBAI YACGTR 1 cut(s) 203
BstC8I GCNNGC 7 cut(s) 57, 61, 711, 1032, 1747, 1966, 1970
BstDSI CCRYGG 2 cut(s) 10, 921
BstEII GGTNACC 1 cut(s) 1229
BstF5I GGATG 3 cut(s) 225, 631, 1996
BstKTI GATC 4 cut(s) 77, 948, 1914, 2050
BstMAI GTCTC 2 cut(s) 132, 1150
BstMBI GATC 4 cut(s) 74, 945, 1911, 2047
BstMWI GCNNNNNNNGC 5 cut(s) 52, 92, 101, 1216, 1949
BstNI CCWGG 3 cut(s) 1397, 1534, 1840
BstNSI RCATGY 7 cut(s) 59, 63, 713, 1034, 1197, 1749, 1768
BstPI GGTNACC 1 cut(s) 1229
BstSCI CCNGG 3 cut(s) 1395, 1532, 1838
BstV1I GCAGC 3 cut(s) 148, 1090, 2156
BstV2I GAAGAC 1 cut(s) 32
BstZ17I GTATAC 2 cut(s) 1199, 1368
Bsu36I CCTNAGG 1 cut(s) 80
BsuRI GGCC 4 cut(s) 1560, 1624, 2126, 2202
BtgI CCRYGG 2 cut(s) 10, 921
BtgZI GCGATG 1 cut(s) 1094
BtrI CACGTC 1 cut(s) 2197
BtsCI GGATG 3 cut(s) 225, 631, 1996
BtsI GCAGTG 2 cut(s) 793, 2196
BtsIMutI CAGTG 5 cut(s) 381, 793, 1803, 2063, 2196
BveI ACCTGC 1 cut(s) 345
Cac8I GCNNGC 7 cut(s) 57, 61, 711, 1032, 1747, 1966, 1970
CaiI CAGNNNCTG 1 cut(s) 1415
CciI TCATGA 1 cut(s) 252
Cfr13I GGNCC 3 cut(s) 394, 884, 2200
Csp6I GTAC 3 cut(s) 71, 824, 975
CspCI CAANNNNNGTGG 4 cut(s) 669, 704, 2161, 2196
CviQI GTAC 3 cut(s) 71, 824, 975
DpnI GATC 4 cut(s) 76, 947, 1913, 2049
DpnII GATC 4 cut(s) 74, 945, 1911, 2047
DraI TTTAAA 2 cut(s) 817, 832
EaeI YGGCCR 2 cut(s) 1558, 1622
Eam1104I CTCTTC 1 cut(s) 1701
EarI CTCTTC 1 cut(s) 1701
Ecl136II GAGCTC 1 cut(s) 1288
Eco130I CCWWGG 2 cut(s) 10, 921
Eco24I GRGCYC 1 cut(s) 1290
Eco31I GGTCTC 1 cut(s) 1150
Eco32I GATATC 1 cut(s) 558
Eco47I GGWCC 2 cut(s) 394, 884
Eco53kI GAGCTC 1 cut(s) 1288
Eco57I CTGAAG 2 cut(s) 26, 660
Eco81I CCTNAGG 1 cut(s) 80
Eco88I CYCGRG 1 cut(s) 182
Eco91I GGTNACC 1 cut(s) 1229
EcoICRI GAGCTC 1 cut(s) 1288
EcoO109I RGGNCCY 2 cut(s) 394, 884
EcoO65I GGTNACC 1 cut(s) 1229
EcoRI GAATTC 3 cut(s) 17, 547, 757
EcoRII CCWGG 3 cut(s) 1395, 1532, 1838
EcoRV GATATC 1 cut(s) 558
EcoT14I CCWWGG 2 cut(s) 10, 921
EcoT22I ATGCAT 1 cut(s) 61
EcoT38I GRGCYC 1 cut(s) 1290
ErhI CCWWGG 2 cut(s) 10, 921
FalI AAGNNNNNCTT 2 cut(s) 1203, 1235
FaqI GGGAC 2 cut(s) 466, 897
FauI CCCGC 1 cut(s) 362
FauNDI CATATG 1 cut(s) 794
FblI GTMKAC 2 cut(s) 1198, 1367
Fnu4HI GCNGC 4 cut(s) 137, 1104, 1561, 2170
FokI GGATG 3 cut(s) 232, 638, 2003
FriOI GRGCYC 1 cut(s) 1290
Fsp4HI GCNGC 4 cut(s) 137, 1104, 1561, 2170
GluI GCNGC 4 cut(s) 137, 1104, 1561, 2170
GsuI CTGGAG 2 cut(s) 948, 1020
HaeIII GGCC 4 cut(s) 1560, 1624, 2126, 2202
HinfI GANTC 5 cut(s) 571, 1004, 1040, 1497, 2176
HphI GGTGA 5 cut(s) 449, 550, 628, 763, 1179
Hpy166II GTNNAC 8 cut(s) 151, 735, 1199, 1368, 1508, 1882, 2068, 2194
Hpy188I TCNGA 4 cut(s) 640, 1347, 1390, 1417
Hpy188III TCNNGA 6 cut(s) 184, 253, 515, 608, 999, 1430
Hpy8I GTNNAC 8 cut(s) 151, 735, 1199, 1368, 1508, 1882, 2068, 2194
HpyAV CCTTC 5 cut(s) 471, 635, 1658, 2137, 2159
HpyCH4III ACNGT 3 cut(s) 429, 705, 971
HpyCH4IV ACGT 4 cut(s) 37, 202, 1752, 2196
HpyF10VI GCNNNNNNNGC 5 cut(s) 52, 92, 101, 1216, 1949
HpySE526I ACGT 4 cut(s) 37, 202, 1752, 2196
KflI GGGWCCC 1 cut(s) 884
Kzo9I GATC 4 cut(s) 74, 945, 1911, 2047
LmnI GCTCC 4 cut(s) 531, 610, 1293, 1327
Lsp1109I GCAGC 3 cut(s) 148, 1090, 2156
LweI GCATC 6 cut(s) 210, 430, 1381, 1572, 1593, 1811
MaeII ACGT 4 cut(s) 37, 202, 1752, 2196
MaeIII GTNAC 7 cut(s) 165, 538, 1169, 1229, 1405, 1733, 2092
MalI GATC 4 cut(s) 76, 947, 1913, 2049
MboI GATC 4 cut(s) 74, 945, 1911, 2047
MboII GAAGA 4 cut(s) 32, 1526, 1688, 1906
MhlI GDGCHC 1 cut(s) 1290
MlsI TGGCCA 1 cut(s) 1624
MluNI TGGCCA 1 cut(s) 1624
MlyI GAGTC 2 cut(s) 1013, 2185
MmeI TCCRAC 2 cut(s) 1315, 1621
Mox20I TGGCCA 1 cut(s) 1624
Mph1103I ATGCAT 1 cut(s) 61
MroXI GAANNNNTTC 1 cut(s) 1218
MscI TGGCCA 1 cut(s) 1624
MslI CAYNNNNRTG 4 cut(s) 116, 673, 1192, 1744
Msp20I TGGCCA 1 cut(s) 1624
MspCI CTTAAG 1 cut(s) 1265
MspR9I CCNGG 3 cut(s) 1397, 1534, 1840
Mva1269I GAATGC 2 cut(s) 403, 1159
MvaI CCWGG 3 cut(s) 1397, 1534, 1840
MwoI GCNNNNNNNGC 5 cut(s) 52, 92, 101, 1216, 1949
NcoI CCATGG 2 cut(s) 10, 921
NdeI CATATG 1 cut(s) 794
NdeII GATC 4 cut(s) 74, 945, 1911, 2047
NlaIV GGNNCC 4 cut(s) 395, 885, 886, 990
NmeAIII GCCGAG 1 cut(s) 779
NmuCI GTSAC 4 cut(s) 165, 538, 1405, 1733
NsiI ATGCAT 1 cut(s) 61
NspI RCATGY 7 cut(s) 59, 63, 713, 1034, 1197, 1749, 1768
PaeI GCATGC 5 cut(s) 59, 63, 713, 1034, 1749
PagI TCATGA 1 cut(s) 252
PciI ACATGT 2 cut(s) 1193, 1764
PctI GAATGC 2 cut(s) 403, 1159
PdmI GAANNNNTTC 1 cut(s) 1218
PfeI GAWTC 3 cut(s) 571, 1040, 1497
PflMI CCANNNNNTGG 2 cut(s) 998, 2062
PfoI TCCNGGA 1 cut(s) 1532
PkrI GCNGC 4 cut(s) 138, 1105, 1562, 2171
PleI GAGTC 2 cut(s) 1012, 2184
PpsI GAGTC 2 cut(s) 1012, 2184
Ppu21I YACGTR 1 cut(s) 203
PpuMI RGGWCCY 2 cut(s) 394, 884
PscI ACATGT 2 cut(s) 1193, 1764
PshBI ATTAAT 1 cut(s) 194
PsiI TTATAA 2 cut(s) 722, 1358
Psp124BI GAGCTC 1 cut(s) 1290
Psp1406I AACGTT 1 cut(s) 1752
Psp5II RGGWCCY 2 cut(s) 394, 884
Psp6I CCWGG 3 cut(s) 1395, 1532, 1838
PspEI GGTNACC 1 cut(s) 1229
PspGI CCWGG 3 cut(s) 1395, 1532, 1838
PspN4I GGNNCC 4 cut(s) 395, 885, 886, 990
PspPI GGNCC 3 cut(s) 394, 884, 2200
PspPPI RGGWCCY 2 cut(s) 394, 884
PstNI CAGNNNCTG 1 cut(s) 1415
RsaI GTAC 3 cut(s) 72, 825, 976
RsaNI GTAC 3 cut(s) 71, 824, 975
RseI CAYNNNNRTG 4 cut(s) 116, 673, 1192, 1744
SacI GAGCTC 1 cut(s) 1290
SatI GCNGC 4 cut(s) 137, 1104, 1561, 2170
Sau3AI GATC 4 cut(s) 74, 945, 1911, 2047
Sau96I GGNCC 3 cut(s) 394, 884, 2200
SchI GAGTC 2 cut(s) 1013, 2185
ScrFI CCNGG 3 cut(s) 1397, 1534, 1840
SduI GDGCHC 1 cut(s) 1290
SfaNI GCATC 6 cut(s) 210, 430, 1381, 1572, 1593, 1811
SinI GGWCC 2 cut(s) 394, 884
SmiMI CAYNNNNRTG 4 cut(s) 116, 673, 1192, 1744
SmlI CTYRAG 2 cut(s) 64, 1265
SmoI CTYRAG 2 cut(s) 64, 1265
SphI GCATGC 5 cut(s) 59, 63, 713, 1034, 1749
SsiI CCGC 3 cut(s) 369, 500, 1561
SspI AATATT 1 cut(s) 173
SstI GAGCTC 1 cut(s) 1290
StyD4I CCNGG 3 cut(s) 1395, 1532, 1838
StyI CCWWGG 2 cut(s) 10, 921
TaaI ACNGT 3 cut(s) 429, 705, 971
TaiI ACGT 4 cut(s) 40, 205, 1755, 2199
TaqI TCGA 1 cut(s) 1175
TatI WGTACW 1 cut(s) 823
TauI GCSGC 1 cut(s) 1563
TfiI GAWTC 3 cut(s) 571, 1040, 1497
TscAI CASTG 5 cut(s) 381, 793, 1803, 2063, 2196
TseFI GTSAC 4 cut(s) 165, 538, 1405, 1733
TseI GCWGC 3 cut(s) 136, 1103, 2169
Tsp45I GTSAC 4 cut(s) 165, 538, 1405, 1733
TspDTI ATGAA 8 cut(s) 663, 770, 1001, 1211, 1239, 1289, 1500, 1688
TspGWI ACGGA 1 cut(s) 438
TspRI CASTG 5 cut(s) 381, 793, 1803, 2063, 2196
Van91I CCANNNNNTGG 2 cut(s) 998, 2062
Vha464I CTTAAG 1 cut(s) 1265
VpaK11BI GGWCC 2 cut(s) 394, 884
VspI ATTAAT 1 cut(s) 194
XapI RAATTY 7 cut(s) 17, 547, 692, 744, 757, 810, 1458
XceI RCATGY 7 cut(s) 59, 63, 713, 1034, 1197, 1749, 1768
XmiI GTMKAC 2 cut(s) 1198, 1367
XmnI GAANNNNTTC 1 cut(s) 1218
Zsp2I ATGCAT 1 cut(s) 61
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.