Rw2G027190

Pentatricopeptide repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr2
OG0010257
Physical Location & Seq
Forward (+)
42436899 .. 42440999
4101 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw2G027190.1

Sequence Viewer

Length: 2208 bp
ATGAGCTTCCATGGAGAATTCCTCAAACATCAAAAACGTCTTTTGCAACTACTGCATGCATGCTCAAGGGTACGATCCCTCAGGGCAACAAAAGCACTCCATGCTCTCAACATCACAATGGGTCTGTCTCCAAAGCAGCCCATCTTTGTTTACAACAATATCATGTCACAATATTCCTCACTCGGGAAGTTATTAGTTGCACGTAAATTGTTTGATAAGATGCCTCTTAGAAACGTTGTGTCATATAATATCATGATTAGTGCTTATTGTACTTCTGGGTATGTTGGGGAAGCTTGGAAAATGTTTTATGAGATGAGGCTTTCTGGGTTTCAGCCAACTCAGTATGCAGTTAGCAGGTTATTGTCATGTGGGTCATTGGATATTCATCATGGGGTCCTGTTGCATTCTTTGGTAATAAAGAACGGACTGTTTGATGGTGATGCTTTTGTGGGGACTTCCTTGTTGGGTTTTTATGGAAGGCATGGGCTGTTAGAGGAAGCGGTTTGGACATTTCAGGATATGCCTTGTAGGAGCTTGGTGACATGGAATTCCATGATATCTTTGCTTGGGAATCATGGGTTTTTTGAAAATTGTGTGTTTTTGTTTCGGGAGCTGGTGAGGAGGGATGTTAATTTGTCTGAAGGTTCTTTTATGGGTGTTCTTTCTGCATTTTCATGCCCGCAAGACTTGGAATTTGGGGAACAGTTGCATGCTTTTGTTATAAAGAATGGGTTTACTTGTGAAATTTTGGTGATGAATTCTCTAATAAGTATGTATGTGAAATGCAGTGCCATATGCTCGGCAGAGAAAATTTTTAAAGAAGTACAATTTAAACATGTTGTATCATGGAATACTATCATTGGTGCGACAGCAAAAATTGGGGGACCCCAAAAAGCAGTAGAACTCTTCTCTCAAATGTCTATGGCTGGAGTGTTACCTACCGAGATCACATTTGTTAGTCTAATAAACTGTTGTACCAGTTTGGAGGTTCCAATCTCTGGACAGTCAGTTCATGCTAAGATAATCCAGCATGCTTATGAATCTAATGTCTTAGTAGGTAGTGCATTGGTTGATTTTTATGCTAAATGTGATAACTTGAAAGCTGCCCATCGCTGTTTTTATGAGATATGTGCGAAAAATGTGGTCTCTTGGAATGCTTTGATTTTGGGTTACTCAAATAGTTCCTCACCTACATGTATACTCTTACTGCAAGAAATGCTTCATTTGGGTTACCGACCTAACGAGTTTTCATTTTCTGCTGTTCTTAAGTCATCATTGGCATTAGAGCTCCAACAACTTCATTGCTTAGTTGTAAGAATGGGCTTTGACCAAAATTATTATGTTCTGAGCTCTCTTATAATGTCGTATACCAAAAGTGGTTTAATATCTGATGCTCTGGTCTTTGTCACAGCTTCTGATGTTCCCTCTAATGCCATTGCTGGAATTTACAACAGAACTGGGCAATATAATGAAACTCTAAGATTCCTTTGTCCACTTGAAGAACTTGATATTTTTTCCTGGAACATTGTGATTTCAGCTCTTGGCCGCATCGGTTATTACAAAGAGGTATTTGAGCTTTTCCAACAGATGCAGTTCAATCAGGTCTGGCCAGACAATTATACATTTGTTAGTCTTCTAAGTGTCTGTACTAAACTTTGTAATCTTGCTTTGGGAAGTTCTCTTCATGCTTATATCATTAAGACTGATTTCAGTTCTTGTGACACATTTGCATGCAACGTTTTACTTGACATGTATGGGAAATGTGGTAGCATTGGAAACTCAGTGAAAATCTTTGACGAAATGAGATACAAAAACTTAATTACCTGGACAGCTTTGATTTCTGCTCTTGGAGTTAATGGTTATGTTCACGAGGCATTAGAAAGATTTAGAGAAATGATCTTCTTGGGCTTTAAGCCTGATGGAGTAGCTTTTACTGCTGTTCTTACAGCTTGCAGGCATGGTGGGTTAGTTAGGGATGGATTGGAGTTATTTGGGCAAATGATAAATAATTATGGGGTTGAACCAAAAATGGATCATTACCAGTGTGTGGTGGACTTACTGGTTAAAGGTGGTCATGTTACAGAAGCAGAGAAACTAATTTCCAGCATGGCCTTCCCACCAAATATGATTATATGGCGTTGTTTCCTTGAAGGCTGCAAGAGTCATGGAAATGCAGTGGACGTGGACCAGGCAGTAGCACATGCTTAA

Protein Analysis

735

Amino Acids

82.07

Weight (kDa)

7.06

Isoelectric Point (pI)

36.38

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PPR_2 PF13041 77 - 121 8.2e-12 PPR repeat family
PPR_1 PF12854 78 - 106 2.1e-08 PPR repeat
PPR PF01535 80 - 110 3e-08 PPR repeat
PPR_2 PF13041 279 - 325 1.9e-10 PPR repeat family
PPR_2 PF13041 503 - 550 4.6e-10 PPR repeat family
PPR_2 PF13041 605 - 651 8.5e-08 PPR repeat family
PPR PF01535 607 - 636 6.6e-06 PPR repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 722, 1358
Acc36I ACCTGC 1 cut(s) 345
AccB7I CCANNNNNTGG 2 cut(s) 998, 2047
AccI GTMKAC 2 cut(s) 1198, 1367
AciI CCGC 3 cut(s) 500, 680, 1546
AclI AACGTT 2 cut(s) 234, 1737
AclWI GGATC 2 cut(s) 69, 2040
AcoI YGGCCR 2 cut(s) 1543, 1607
AcsI RAATTY 7 cut(s) 17, 547, 692, 744, 757, 810, 1443
AcuI CTGAAG 1 cut(s) 660
AfaI GTAC 5 cut(s) 72, 271, 825, 976, 1648
AfiI CCNNNNNNNGG 3 cut(s) 183, 998, 2047
AflII CTTAAG 1 cut(s) 1265
AflIII ACRYGT 3 cut(s) 835, 1193, 1749
AgsI TTSAA 6 cut(s) 587, 1099, 1499, 1597, 2021, 2150
AjiI CACGTC 1 cut(s) 2182
AjnI CCWGG 3 cut(s) 1517, 1823, 2187
AloI GAACNNNNNNTCC 2 cut(s) 993, 1025
Alw21I GWGCWC 2 cut(s) 1290, 1352
Alw26I GTCTC 2 cut(s) 132, 1150
AlwI GGATC 2 cut(s) 69, 2040
AlwNI CAGNNNCTG 1 cut(s) 1415
Ama87I CYCGRG 1 cut(s) 182
AoxI GGCC 3 cut(s) 1543, 1607, 2109
ApeKI GCWGC 3 cut(s) 136, 1103, 2154
ApoI RAATTY 7 cut(s) 17, 547, 692, 744, 757, 810, 1443
ArsI GACNNNNNNTTYG 2 cut(s) 677, 709
Asp700I GAANNNNTTC 1 cut(s) 1218
AspS9I GGNCC 3 cut(s) 394, 884, 2185
AsuHPI GGTGA 5 cut(s) 449, 550, 628, 763, 1179
AvaI CYCGRG 1 cut(s) 182
AvaII GGWCC 3 cut(s) 394, 884, 2185
AxyI CCTNAGG 1 cut(s) 80
BaeI ACNNNNGTAYC 2 cut(s) 958, 991
BalI TGGCCA 1 cut(s) 1609
BanII GRGCYC 2 cut(s) 1290, 1352
BauI CACGAG 1 cut(s) 1868
BbsI GAAGAC 1 cut(s) 1625
Bbv12I GWGCWC 2 cut(s) 1290, 1352
BbvI GCAGC 3 cut(s) 148, 1090, 2141
BccI CCATC 5 cut(s) 149, 428, 1116, 1913, 1970
BciT130I CCWGG 3 cut(s) 1519, 1825, 2189
BcoDI GTCTC 2 cut(s) 132, 1150
BfrI CTTAAG 1 cut(s) 1265
BfuAI ACCTGC 1 cut(s) 345
BisI GCNGC 4 cut(s) 137, 1104, 1546, 2155
BlsI GCNGC 4 cut(s) 138, 1105, 1547, 2156
Bme1390I CCNGG 3 cut(s) 1519, 1825, 2189
Bme18I GGWCC 3 cut(s) 394, 884, 2185
BmeT110I CYCGRG 1 cut(s) 182
BmgBI CACGTC 1 cut(s) 2182
BmgT120I GGNCC 3 cut(s) 394, 884, 2185
BmiI GGNNCC 4 cut(s) 395, 885, 886, 990
BmrFI CCNGG 3 cut(s) 1519, 1825, 2189
BmrI ACTGGG 1 cut(s) 1467
BmsI GCATC 5 cut(s) 210, 430, 1381, 1557, 1578
BmuI ACTGGG 1 cut(s) 1467
BpiI GAAGAC 1 cut(s) 1625
BplI GAGNNNNNCTC 1 cut(s) 38
BpmI CTGGAG 1 cut(s) 948
BpuEI CTTGAG 1 cut(s) 49
BsaAI YACGTR 1 cut(s) 203
BsaBI GATNNNNATC 1 cut(s) 384
BsaI GGTCTC 1 cut(s) 1150
BsaJI CCNNGG 1 cut(s) 10
Bsc4I CCNNNNNNNGG 3 cut(s) 183, 998, 2047
Bse1I ACTGG 4 cut(s) 978, 1462, 2041, 2064
Bse21I CCTNAGG 1 cut(s) 80
Bse3DI GCAATG 2 cut(s) 1300, 1434
Bse8I GATNNNNATC 1 cut(s) 384
BseBI CCWGG 3 cut(s) 1519, 1825, 2189
BseDI CCNNGG 1 cut(s) 10
BseGI GGATG 2 cut(s) 631, 1981
BseJI GATNNNNATC 1 cut(s) 384
BseLI CCNNNNNNNGG 3 cut(s) 183, 998, 2047
BseMI GCAATG 2 cut(s) 1300, 1434
BseMII CTCAG 4 cut(s) 94, 353, 1337, 1794
BseNI ACTGG 4 cut(s) 978, 1462, 2041, 2064
BseRI GAGGAG 1 cut(s) 634
BseXI GCAGC 3 cut(s) 148, 1090, 2141
BshFI GGCC 3 cut(s) 1545, 1609, 2111
BsiHKAI GWGCWC 2 cut(s) 1290, 1352
BsiHKCI CYCGRG 1 cut(s) 182
BslFI GGGAC 2 cut(s) 466, 897
BslI CCNNNNNNNGG 3 cut(s) 183, 998, 2047
BsmAI GTCTC 2 cut(s) 132, 1150
BsmFI GGGAC 2 cut(s) 466, 897
BsmI GAATGC 2 cut(s) 403, 1159
BsnI GGCC 3 cut(s) 1545, 1609, 2111
Bso31I GGTCTC 1 cut(s) 1150
BsoBI CYCGRG 1 cut(s) 182
Bsp1286I GDGCHC 2 cut(s) 1290, 1352
Bsp143I GATC 4 cut(s) 74, 945, 1896, 2032
Bsp19I CCATGG 1 cut(s) 10
BspACI CCGC 3 cut(s) 500, 680, 1546
BspANI GGCC 3 cut(s) 1545, 1609, 2111
BspCNI CTCAG 4 cut(s) 93, 352, 1338, 1793
BspHI TCATGA 1 cut(s) 252
BspLI GGNNCC 4 cut(s) 395, 885, 886, 990
BspMI ACCTGC 1 cut(s) 345
BspPI GGATC 2 cut(s) 69, 2040
BspTI CTTAAG 1 cut(s) 1265
BspTNI GGTCTC 1 cut(s) 1150
BsrDI GCAATG 2 cut(s) 1300, 1434
BsrI ACTGG 4 cut(s) 978, 1462, 2041, 2064
BssECI CCNNGG 1 cut(s) 10
BssMI GATC 4 cut(s) 74, 945, 1896, 2032
BssNAI GTATAC 2 cut(s) 1199, 1368
BssSI CACGAG 1 cut(s) 1868
BssT1I CCWWGG 1 cut(s) 10
Bst1107I GTATAC 2 cut(s) 1199, 1368
Bst2BI CACGAG 1 cut(s) 1868
Bst2UI CCWGG 3 cut(s) 1519, 1825, 2189
Bst4CI ACNGT 4 cut(s) 429, 705, 971, 1005
Bst6I CTCTTC 2 cut(s) 911, 1686
BstAFI CTTAAG 1 cut(s) 1265
BstAPI GCANNNNNTGC 3 cut(s) 52, 101, 1216
BstBAI YACGTR 1 cut(s) 203
BstC8I GCNNGC 8 cut(s) 57, 61, 680, 711, 1032, 1732, 1951, 1955
BstDSI CCRYGG 1 cut(s) 10
BstEII GGTNACC 1 cut(s) 1229
BstF5I GGATG 2 cut(s) 631, 1981
BstKTI GATC 4 cut(s) 77, 948, 1899, 2035
BstMAI GTCTC 2 cut(s) 132, 1150
BstMBI GATC 4 cut(s) 74, 945, 1896, 2032
BstMWI GCNNNNNNNGC 5 cut(s) 52, 92, 101, 1216, 1934
BstNI CCWGG 3 cut(s) 1519, 1825, 2189
BstNSI RCATGY 9 cut(s) 59, 63, 713, 839, 1034, 1197, 1734, 1753, 2204
BstPI GGTNACC 1 cut(s) 1229
BstSCI CCNGG 3 cut(s) 1517, 1823, 2187
BstV1I GCAGC 3 cut(s) 148, 1090, 2141
BstV2I GAAGAC 1 cut(s) 1625
BstZ17I GTATAC 2 cut(s) 1199, 1368
Bsu36I CCTNAGG 1 cut(s) 80
BsuRI GGCC 3 cut(s) 1545, 1609, 2111
BtgI CCRYGG 1 cut(s) 10
BtgZI GCGATG 1 cut(s) 1094
BtrI CACGTC 1 cut(s) 2182
BtsCI GGATG 2 cut(s) 631, 1981
BtsI GCAGTG 2 cut(s) 793, 2181
BtsIMutI CAGTG 4 cut(s) 793, 1788, 2048, 2181
BveI ACCTGC 1 cut(s) 345
Cac8I GCNNGC 8 cut(s) 57, 61, 680, 711, 1032, 1732, 1951, 1955
CaiI CAGNNNCTG 1 cut(s) 1415
CciI TCATGA 1 cut(s) 252
Cfr13I GGNCC 3 cut(s) 394, 884, 2185
Csp6I GTAC 5 cut(s) 71, 270, 824, 975, 1647
CviQI GTAC 5 cut(s) 71, 270, 824, 975, 1647
DpnI GATC 4 cut(s) 76, 947, 1898, 2034
DpnII GATC 4 cut(s) 74, 945, 1896, 2032
DraI TTTAAA 2 cut(s) 817, 832
EaeI YGGCCR 2 cut(s) 1543, 1607
Eam1104I CTCTTC 2 cut(s) 911, 1686
EarI CTCTTC 2 cut(s) 911, 1686
Ecl136II GAGCTC 2 cut(s) 1288, 1350
Eco130I CCWWGG 1 cut(s) 10
Eco24I GRGCYC 2 cut(s) 1290, 1352
Eco31I GGTCTC 1 cut(s) 1150
Eco32I GATATC 1 cut(s) 558
Eco47I GGWCC 3 cut(s) 394, 884, 2185
Eco53kI GAGCTC 2 cut(s) 1288, 1350
Eco57I CTGAAG 1 cut(s) 660
Eco81I CCTNAGG 1 cut(s) 80
Eco88I CYCGRG 1 cut(s) 182
Eco91I GGTNACC 1 cut(s) 1229
EcoICRI GAGCTC 2 cut(s) 1288, 1350
EcoO109I RGGNCCY 2 cut(s) 394, 884
EcoO65I GGTNACC 1 cut(s) 1229
EcoRI GAATTC 3 cut(s) 17, 547, 757
EcoRII CCWGG 3 cut(s) 1517, 1823, 2187
EcoRV GATATC 1 cut(s) 558
EcoT14I CCWWGG 1 cut(s) 10
EcoT22I ATGCAT 1 cut(s) 61
EcoT38I GRGCYC 2 cut(s) 1290, 1352
ErhI CCWWGG 1 cut(s) 10
FalI AAGNNNNNCTT 2 cut(s) 1203, 1235
FaqI GGGAC 2 cut(s) 466, 897
FauI CCCGC 1 cut(s) 687
FauNDI CATATG 1 cut(s) 794
FblI GTMKAC 2 cut(s) 1198, 1367
Fnu4HI GCNGC 4 cut(s) 137, 1104, 1546, 2155
FokI GGATG 2 cut(s) 638, 1988
FriOI GRGCYC 2 cut(s) 1290, 1352
Fsp4HI GCNGC 4 cut(s) 137, 1104, 1546, 2155
GluI GCNGC 4 cut(s) 137, 1104, 1546, 2155
GsuI CTGGAG 1 cut(s) 948
HaeIII GGCC 3 cut(s) 1545, 1609, 2111
HindIII AAGCTT 1 cut(s) 291
HinfI GANTC 4 cut(s) 571, 1040, 1482, 2161
HphI GGTGA 5 cut(s) 449, 550, 628, 763, 1179
Hpy166II GTNNAC 9 cut(s) 151, 735, 1199, 1368, 1493, 1867, 2053, 2179, 2185
Hpy188I TCNGA 4 cut(s) 640, 1347, 1390, 1417
Hpy188III TCNNGA 6 cut(s) 184, 253, 515, 608, 999, 1868
Hpy8I GTNNAC 9 cut(s) 151, 735, 1199, 1368, 1493, 1867, 2053, 2179, 2185
HpyAV CCTTC 4 cut(s) 471, 635, 2122, 2144
HpyCH4III ACNGT 4 cut(s) 429, 705, 971, 1005
HpyCH4IV ACGT 5 cut(s) 37, 202, 234, 1737, 2181
HpyF10VI GCNNNNNNNGC 5 cut(s) 52, 92, 101, 1216, 1934
HpySE526I ACGT 5 cut(s) 37, 202, 234, 1737, 2181
KflI GGGWCCC 1 cut(s) 884
Kzo9I GATC 4 cut(s) 74, 945, 1896, 2032
LmnI GCTCC 3 cut(s) 531, 610, 1293
Lsp1109I GCAGC 3 cut(s) 148, 1090, 2141
LweI GCATC 5 cut(s) 210, 430, 1381, 1557, 1578
MaeII ACGT 5 cut(s) 37, 202, 234, 1737, 2181
MaeIII GTNAC 8 cut(s) 165, 538, 933, 1169, 1229, 1405, 1718, 2077
MalI GATC 4 cut(s) 76, 947, 1898, 2034
MboI GATC 4 cut(s) 74, 945, 1896, 2032
MboII GAAGA 5 cut(s) 898, 1511, 1625, 1673, 1891
MhlI GDGCHC 2 cut(s) 1290, 1352
MlsI TGGCCA 1 cut(s) 1609
MluNI TGGCCA 1 cut(s) 1609
MlyI GAGTC 1 cut(s) 2170
MmeI TCCRAC 2 cut(s) 1315, 1606
Mox20I TGGCCA 1 cut(s) 1609
Mph1103I ATGCAT 1 cut(s) 61
MroXI GAANNNNTTC 1 cut(s) 1218
MscI TGGCCA 1 cut(s) 1609
MslI CAYNNNNRTG 6 cut(s) 116, 673, 1035, 1192, 1729, 2169
Msp20I TGGCCA 1 cut(s) 1609
MspCI CTTAAG 1 cut(s) 1265
MspR9I CCNGG 3 cut(s) 1519, 1825, 2189
Mva1269I GAATGC 2 cut(s) 403, 1159
MvaI CCWGG 3 cut(s) 1519, 1825, 2189
MwoI GCNNNNNNNGC 5 cut(s) 52, 92, 101, 1216, 1934
NcoI CCATGG 1 cut(s) 10
NdeI CATATG 1 cut(s) 794
NdeII GATC 4 cut(s) 74, 945, 1896, 2032
NlaIV GGNNCC 4 cut(s) 395, 885, 886, 990
NmeAIII GCCGAG 1 cut(s) 779
NmuCI GTSAC 4 cut(s) 165, 538, 1405, 1718
NsiI ATGCAT 1 cut(s) 61
NspI RCATGY 9 cut(s) 59, 63, 713, 839, 1034, 1197, 1734, 1753, 2204
PaeI GCATGC 5 cut(s) 59, 63, 713, 1034, 1734
PagI TCATGA 1 cut(s) 252
PciI ACATGT 3 cut(s) 835, 1193, 1749
PctI GAATGC 2 cut(s) 403, 1159
PdmI GAANNNNTTC 1 cut(s) 1218
PfeI GAWTC 3 cut(s) 571, 1040, 1482
PflMI CCANNNNNTGG 2 cut(s) 998, 2047
PfoI TCCNGGA 1 cut(s) 1517
PkrI GCNGC 4 cut(s) 138, 1105, 1547, 2156
PleI GAGTC 1 cut(s) 2169
PpsI GAGTC 1 cut(s) 2169
Ppu21I YACGTR 1 cut(s) 203
PpuMI RGGWCCY 2 cut(s) 394, 884
PscI ACATGT 3 cut(s) 835, 1193, 1749
PsiI TTATAA 2 cut(s) 722, 1358
Psp124BI GAGCTC 2 cut(s) 1290, 1352
Psp1406I AACGTT 2 cut(s) 234, 1737
Psp5II RGGWCCY 2 cut(s) 394, 884
Psp6I CCWGG 3 cut(s) 1517, 1823, 2187
PspEI GGTNACC 1 cut(s) 1229
PspGI CCWGG 3 cut(s) 1517, 1823, 2187
PspN4I GGNNCC 4 cut(s) 395, 885, 886, 990
PspPI GGNCC 3 cut(s) 394, 884, 2185
PspPPI RGGWCCY 2 cut(s) 394, 884
PstNI CAGNNNCTG 1 cut(s) 1415
RsaI GTAC 5 cut(s) 72, 271, 825, 976, 1648
RsaNI GTAC 5 cut(s) 71, 270, 824, 975, 1647
RseI CAYNNNNRTG 6 cut(s) 116, 673, 1035, 1192, 1729, 2169
SacI GAGCTC 2 cut(s) 1290, 1352
SatI GCNGC 4 cut(s) 137, 1104, 1546, 2155
Sau3AI GATC 4 cut(s) 74, 945, 1896, 2032
Sau96I GGNCC 3 cut(s) 394, 884, 2185
SchI GAGTC 1 cut(s) 2170
ScrFI CCNGG 3 cut(s) 1519, 1825, 2189
SduI GDGCHC 2 cut(s) 1290, 1352
SfaNI GCATC 5 cut(s) 210, 430, 1381, 1557, 1578
SinI GGWCC 3 cut(s) 394, 884, 2185
SmiMI CAYNNNNRTG 6 cut(s) 116, 673, 1035, 1192, 1729, 2169
SmlI CTYRAG 2 cut(s) 64, 1265
SmoI CTYRAG 2 cut(s) 64, 1265
SphI GCATGC 5 cut(s) 59, 63, 713, 1034, 1734
SsiI CCGC 3 cut(s) 500, 680, 1546
SspI AATATT 1 cut(s) 173
SstI GAGCTC 2 cut(s) 1290, 1352
StyD4I CCNGG 3 cut(s) 1517, 1823, 2187
StyI CCWWGG 1 cut(s) 10
TaaI ACNGT 4 cut(s) 429, 705, 971, 1005
TaiI ACGT 5 cut(s) 40, 205, 237, 1740, 2184
TatI WGTACW 3 cut(s) 269, 823, 1646
TauI GCSGC 1 cut(s) 1548
TfiI GAWTC 3 cut(s) 571, 1040, 1482
TscAI CASTG 4 cut(s) 793, 1788, 2048, 2181
TseFI GTSAC 4 cut(s) 165, 538, 1405, 1718
TseI GCWGC 3 cut(s) 136, 1103, 2154
Tsp45I GTSAC 4 cut(s) 165, 538, 1405, 1718
TspGWI ACGGA 1 cut(s) 438
TspRI CASTG 4 cut(s) 793, 1788, 2048, 2181
Van91I CCANNNNNTGG 2 cut(s) 998, 2047
Vha464I CTTAAG 1 cut(s) 1265
VpaK11BI GGWCC 3 cut(s) 394, 884, 2185
XapI RAATTY 7 cut(s) 17, 547, 692, 744, 757, 810, 1443
XceI RCATGY 9 cut(s) 59, 63, 713, 839, 1034, 1197, 1734, 1753, 2204
XmiI GTMKAC 2 cut(s) 1198, 1367
XmnI GAANNNNTTC 1 cut(s) 1218
Zsp2I ATGCAT 1 cut(s) 61
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.