RchiOBHm_Chr2g0153211

Ribonuclease H protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
2
OG0018062
Physical Location & Seq
Forward (+)
70634957 .. 70637139
2183 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 591 bp
ATGGCTGCAGCACCTATCCAAAAGTTTATGGTTACTGAAAATGAAATATTTATTCATAAGATTGTATGGACACGTCCATTCGCAGGGTCATGGAAGTTAAACTACGCCTATGCTTATAACCAGACTTCAAGAAAAGCTTTTGTTGGCTGTGTTATAAGATTTGAAAATGCAGCGTTTAAAGCTCTCTTGGCAGGAGCGGCAGGTCAGGACGCAAGGTCAACTTCAGACGCTGCATTGATGGCCTTGACTGCTGCTGTTGGCTTAGCAGTAAGTCAAGGTGGAGACTTTCTCGAAATAGAGGGAGAGAATGAAACTACGTTCTCCTTGGTCACAGGCCAGACCCCTCCATCAGATGAACTGACAAGGGAGCTTATGTTTAGGTGCCTAAGGGAGCTGCAGAATTTCAGAAGGGTGGAGTTTCGCCATGTGAAACCTGACACCAATGAGGCGGCCAACCACCTAGCTGGAATGGCCATGGAGACGGAGGAGAGCTGGTTTTATGCAGACAACCCTCCACCTGAAGTAGCTGCAATTCTGGATGCTGATGTCAATGGTCGTTATGTGCTATGGGAGCATCCTAGCAATCTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RVT_1 RVT_3

Protein Analysis

196

Amino Acids

21.79

Weight (kDa)

5.02

Isoelectric Point (pI)

39.82

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RVT_3 PF13456 34 - 158 1.2e-13 Reverse transcriptase-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0018062)

Species Orthologous Gene IDs
fragaria_vesca FvH4_6g39290 FvH4_6g39290 FvH4_6g39290
rosa_chinensis RchiOBHm_Chr2g0153211
rosa_laevigata RLG00000020686
rosa_roxburghii Rroxscaffold_2G00095110
rosa_rugosa Rorug02G0437500
rosa_samantha Rh2AG500500 Rh2BG511900 Rh2CG486700 Rh2DG523900

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 117, 155
Acc36I ACCTGC 1 cut(s) 191
AccB1I GGYRCC 1 cut(s) 381
AccBSI CCGCTC 1 cut(s) 197
AciI CCGC 2 cut(s) 197, 449
AcoI YGGCCR 2 cut(s) 450, 471
AcsI RAATTY 1 cut(s) 400
AcuI CTGAAG 2 cut(s) 207, 540
AfiI CCNNNNNNNGG 1 cut(s) 83
AflIII ACRYGT 1 cut(s) 71
AgsI TTSAA 2 cut(s) 129, 164
AhdI GACNNNNNGTC 1 cut(s) 214
AjiI CACGTC 1 cut(s) 74
AluBI AGCT 7 cut(s) 137, 182, 370, 394, 464, 492, 527
AluI AGCT 7 cut(s) 137, 182, 370, 394, 464, 492, 527
Alw26I GTCTC 2 cut(s) 276, 473
AlwNI CAGNNNCTG 1 cut(s) 230
AoxI GGCC 4 cut(s) 240, 334, 450, 471
ApeKI GCWGC 7 cut(s) 5, 8, 170, 230, 251, 394, 527
ApoI RAATTY 1 cut(s) 400
AxyI CCTNAGG 1 cut(s) 386
BalI TGGCCA 1 cut(s) 473
BanI GGYRCC 1 cut(s) 381
BarI GAAGNNNNNNTAC 2 cut(s) 86, 118
BbvI GCAGC 6 cut(s) 20, 182, 217, 238, 381, 514
BccI CCATC 2 cut(s) 232, 355
BcoDI GTCTC 2 cut(s) 276, 473
BfaI CTAG 2 cut(s) 461, 579
BfmI CTRYAG 2 cut(s) 6, 395
BfuAI ACCTGC 1 cut(s) 191
BisI GCNGC 9 cut(s) 6, 9, 171, 198, 231, 252, 395, 450, 528
BlpI GCTNAGC 1 cut(s) 262
BlsI GCNGC 9 cut(s) 7, 10, 172, 199, 232, 253, 396, 451, 529
BmeRI GACNNNNNGTC 1 cut(s) 214
BmgBI CACGTC 1 cut(s) 74
BmiI GGNNCC 1 cut(s) 383
BmsI GCATC 2 cut(s) 529, 583
BplI GAGNNNNNCTC 2 cut(s) 273, 305
Bpu1102I GCTNAGC 1 cut(s) 262
BsaJI CCNNGG 2 cut(s) 324, 474
BsaXI ACNNNNNCTCC 4 cut(s) 359, 389, 479, 509
Bsc4I CCNNNNNNNGG 1 cut(s) 83
Bse21I CCTNAGG 1 cut(s) 386
BseDI CCNNGG 2 cut(s) 324, 474
BseGI GGATG 2 cut(s) 544, 574
BseLI CCNNNNNNNGG 1 cut(s) 83
BseRI GAGGAG 1 cut(s) 500
BseXI GCAGC 6 cut(s) 20, 182, 217, 238, 381, 514
BshFI GGCC 4 cut(s) 242, 336, 452, 473
BshNI GGYRCC 1 cut(s) 381
BslI CCNNNNNNNGG 1 cut(s) 83
BsmAI GTCTC 2 cut(s) 276, 473
BsmBI CGTCTC 1 cut(s) 473
BsnI GGCC 4 cut(s) 242, 336, 452, 473
Bsp1720I GCTNAGC 1 cut(s) 262
Bsp19I CCATGG 1 cut(s) 474
BspACI CCGC 2 cut(s) 197, 449
BspANI GGCC 4 cut(s) 242, 336, 452, 473
BspLI GGNNCC 1 cut(s) 383
BspMAI CTGCAG 2 cut(s) 10, 399
BspMI ACCTGC 1 cut(s) 191
BspT107I GGYRCC 1 cut(s) 381
BsrBI CCGCTC 1 cut(s) 197
BssECI CCNNGG 2 cut(s) 324, 474
BssT1I CCWWGG 2 cut(s) 324, 474
BstDEI CTNAG 2 cut(s) 262, 386
BstDSI CCRYGG 1 cut(s) 474
BstF5I GGATG 2 cut(s) 544, 574
BstMAI GTCTC 2 cut(s) 276, 473
BstMWI GCNNNNNNNGC 7 cut(s) 179, 188, 197, 239, 248, 470, 571
BstSFI CTRYAG 2 cut(s) 6, 395
BstV1I GCAGC 6 cut(s) 20, 182, 217, 238, 381, 514
BstXI CCANNNNNNTGG 1 cut(s) 464
Bsu36I CCTNAGG 1 cut(s) 386
BsuRI GGCC 4 cut(s) 242, 336, 452, 473
BtgI CCRYGG 1 cut(s) 474
BtrI CACGTC 1 cut(s) 74
BtsCI GGATG 2 cut(s) 544, 574
BveI ACCTGC 1 cut(s) 191
CaiI CAGNNNCTG 1 cut(s) 230
CseI GACGC 2 cut(s) 218, 236
CviAII CATG 3 cut(s) 90, 425, 475
DdeI CTNAG 2 cut(s) 262, 386
DraI TTTAAA 1 cut(s) 178
DriI GACNNNNNGTC 1 cut(s) 214
EaeI YGGCCR 2 cut(s) 450, 471
Eam1105I GACNNNNNGTC 1 cut(s) 214
Eco130I CCWWGG 2 cut(s) 324, 474
Eco57I CTGAAG 2 cut(s) 207, 540
Eco81I CCTNAGG 1 cut(s) 386
EcoT14I CCWWGG 2 cut(s) 324, 474
ErhI CCWWGG 2 cut(s) 324, 474
Esp3I CGTCTC 1 cut(s) 473
FaeI CATG 3 cut(s) 93, 428, 478
FalI AAGNNNNNCTT 4 cut(s) 121, 153, 205, 237
FatI CATG 3 cut(s) 89, 424, 474
Fnu4HI GCNGC 9 cut(s) 6, 9, 171, 198, 231, 252, 395, 450, 528
FokI GGATG 2 cut(s) 551, 561
Fsp4HI GCNGC 9 cut(s) 6, 9, 171, 198, 231, 252, 395, 450, 528
FspBI CTAG 2 cut(s) 461, 579
GluI GCNGC 9 cut(s) 6, 9, 171, 198, 231, 252, 395, 450, 528
HaeIII GGCC 4 cut(s) 242, 336, 452, 473
HgaI GACGC 2 cut(s) 218, 236
Hin1II CATG 3 cut(s) 93, 428, 478
HincII GTYRAC 1 cut(s) 219
HindII GTYRAC 1 cut(s) 219
HindIII AAGCTT 1 cut(s) 135
Hpy166II GTNNAC 1 cut(s) 219
Hpy188I TCNGA 3 cut(s) 226, 352, 407
Hpy188III TCNNGA 4 cut(s) 129, 206, 290, 536
Hpy8I GTNNAC 1 cut(s) 219
HpyAV CCTTC 1 cut(s) 402
HpyCH4IV ACGT 2 cut(s) 73, 317
HpyCH4V TGCA 6 cut(s) 8, 170, 233, 397, 503, 530
HpyF10VI GCNNNNNNNGC 7 cut(s) 179, 188, 197, 239, 248, 470, 571
HpyF3I CTNAG 2 cut(s) 262, 386
HpySE526I ACGT 2 cut(s) 73, 317
Hsp92II CATG 3 cut(s) 93, 428, 478
LmnI GCTCC 4 cut(s) 194, 367, 391, 571
Lsp1109I GCAGC 6 cut(s) 20, 182, 217, 238, 381, 514
LweI GCATC 2 cut(s) 529, 583
MaeI CTAG 2 cut(s) 461, 579
MaeII ACGT 2 cut(s) 73, 317
MaeIII GTNAC 2 cut(s) 31, 328
MbiI CCGCTC 1 cut(s) 197
MlsI TGGCCA 1 cut(s) 473
MluCI AATT 2 cut(s) 400, 531
MluNI TGGCCA 1 cut(s) 473
MnlI CCTC 5 cut(s) 292, 354, 439, 478, 522
Mox20I TGGCCA 1 cut(s) 473
MscI TGGCCA 1 cut(s) 473
MseI TTAA 2 cut(s) 98, 177
Msp20I TGGCCA 1 cut(s) 473
MwoI GCNNNNNNNGC 7 cut(s) 179, 188, 197, 239, 248, 470, 571
NcoI CCATGG 1 cut(s) 474
NlaIII CATG 3 cut(s) 93, 428, 478
NlaIV GGNNCC 1 cut(s) 383
NmuCI GTSAC 1 cut(s) 328
PkrI GCNGC 9 cut(s) 7, 10, 172, 199, 232, 253, 396, 451, 529
PsiI TTATAA 2 cut(s) 117, 155
PspN4I GGNNCC 1 cut(s) 383
PstI CTGCAG 2 cut(s) 10, 399
PstNI CAGNNNCTG 1 cut(s) 230
SaqAI TTAA 2 cut(s) 98, 177
SatI GCNGC 9 cut(s) 6, 9, 171, 198, 231, 252, 395, 450, 528
SfaNI GCATC 2 cut(s) 529, 583
SfcI CTRYAG 2 cut(s) 6, 395
Sse9I AATT 2 cut(s) 400, 531
SsiI CCGC 2 cut(s) 197, 449
SspI AATATT 1 cut(s) 48
SspMI CTAG 2 cut(s) 461, 579
StyI CCWWGG 2 cut(s) 324, 474
TaiI ACGT 2 cut(s) 76, 320
TaqI TCGA 1 cut(s) 291
TasI AATT 2 cut(s) 400, 531
TauI GCSGC 2 cut(s) 200, 452
Tru1I TTAA 2 cut(s) 98, 177
Tru9I TTAA 2 cut(s) 98, 177
TseFI GTSAC 1 cut(s) 328
TseI GCWGC 7 cut(s) 5, 8, 170, 230, 251, 394, 527
Tsp45I GTSAC 1 cut(s) 328
TspDTI ATGAA 4 cut(s) 44, 57, 324, 369
TspGWI ACGGA 1 cut(s) 497
XapI RAATTY 1 cut(s) 400
XspI CTAG 2 cut(s) 461, 579
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.