Rroxscaffold_2G00095110

Ribonuclease H protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000002
OG0018062
Physical Location & Seq
Reverse (-)
16424529 .. 16426652
2124 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_2G00095110.1

Sequence Viewer

Length: 465 bp
ATGCCCCGGTATGCTAACATATTAATATATGAAGTTACTGCTAGAGAGTTTAAAGCTCTCTTGGTAGGAGCGGCGGGTCAGGAGGCAAGGTCAACTACAGATGCTGCATTGATGGCCTTGACTTCTGCTGTTGGCTTAGCAGCAAGTCAAGGTGGAGGGTTCCTCGAAATAGAAGGAGAGAATGAAACTACCTTCTCCATGGTCACAGGCCAGATCCCTCCATCAGATGAAGTGGCAAAGGAGCTTCTGTTTAGGTGCCGAAGGGAGCTGCCAAATTTCAGAAGCGTGGAGTTTCGCCATGTGAAACCTGACACCAATGAGGCGGCCAACCACCTGGCCAGAATGGCCATGGAGACGGAGGAGAGCTGGTTTTATGCAGACAACCCTCCACCTGAAGTAGCTGCAATTCTGGATGCAGATGTCAATGGTCGTTGTGTGCCATGGGAGCATCCTAGCAATCTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RVT_1 RVT_3

Protein Analysis

154

Amino Acids

16.92

Weight (kDa)

4.71

Isoelectric Point (pI)

44.98

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RVT_3 PF13456 22 - 116 1.5e-09 Reverse transcriptase-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0018062)

Species Orthologous Gene IDs
fragaria_vesca FvH4_6g39290 FvH4_6g39290 FvH4_6g39290
rosa_chinensis RchiOBHm_Chr2g0153211
rosa_laevigata RLG00000020686
rosa_roxburghii Rroxscaffold_2G00095110
rosa_rugosa Rorug02G0437500
rosa_samantha Rh2AG500500 Rh2BG511900 Rh2CG486700 Rh2DG523900

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 255
AccBSI CCGCTC 1 cut(s) 71
AciI CCGC 3 cut(s) 71, 74, 323
AclWI GGATC 1 cut(s) 208
AcoI YGGCCR 3 cut(s) 324, 336, 345
AcsI RAATTY 1 cut(s) 274
AcuI CTGAAG 1 cut(s) 414
AjnI CCWGG 1 cut(s) 333
AluBI AGCT 5 cut(s) 56, 244, 268, 366, 401
AluI AGCT 5 cut(s) 56, 244, 268, 366, 401
Alw26I GTCTC 1 cut(s) 347
AlwI GGATC 1 cut(s) 208
AlwNI CAGNNNCTG 1 cut(s) 104
AoxI GGCC 5 cut(s) 114, 208, 324, 336, 345
ApeKI GCWGC 4 cut(s) 104, 140, 268, 401
ApoI RAATTY 1 cut(s) 274
AseI ATTAAT 1 cut(s) 23
AsuC2I CCSGG 1 cut(s) 7
BalI TGGCCA 2 cut(s) 338, 347
BanI GGYRCC 1 cut(s) 255
BbvI GCAGC 4 cut(s) 91, 152, 255, 388
BccI CCATC 2 cut(s) 106, 229
BciT130I CCWGG 1 cut(s) 335
BcnI CCSGG 1 cut(s) 7
BcoDI GTCTC 1 cut(s) 347
BfaI CTAG 2 cut(s) 42, 453
BfmI CTRYAG 1 cut(s) 96
BglI GCCNNNNNGGC 1 cut(s) 344
BisI GCNGC 6 cut(s) 72, 105, 141, 269, 324, 402
BlpI GCTNAGC 1 cut(s) 136
BlsI GCNGC 6 cut(s) 73, 106, 142, 270, 325, 403
Bme1390I CCNGG 2 cut(s) 7, 335
BmiI GGNNCC 2 cut(s) 161, 257
BmrFI CCNGG 2 cut(s) 7, 335
BmsI GCATC 3 cut(s) 91, 403, 457
BplI GAGNNNNNCTC 2 cut(s) 147, 179
Bpu1102I GCTNAGC 1 cut(s) 136
BpuMI CCSGG 1 cut(s) 7
BsaJI CCNNGG 4 cut(s) 5, 198, 348, 440
BsaXI ACNNNNNCTCC 6 cut(s) 74, 104, 233, 263, 353, 383
BseBI CCWGG 1 cut(s) 335
BseDI CCNNGG 4 cut(s) 5, 198, 348, 440
BseGI GGATG 2 cut(s) 418, 448
BseRI GAGGAG 1 cut(s) 374
BseXI GCAGC 4 cut(s) 91, 152, 255, 388
BshFI GGCC 5 cut(s) 116, 210, 326, 338, 347
BshNI GGYRCC 1 cut(s) 255
BsiSI CCGG 1 cut(s) 7
BsmAI GTCTC 1 cut(s) 347
BsmBI CGTCTC 1 cut(s) 347
BsnI GGCC 5 cut(s) 116, 210, 326, 338, 347
Bsp143I GATC 1 cut(s) 213
Bsp1720I GCTNAGC 1 cut(s) 136
Bsp19I CCATGG 3 cut(s) 198, 348, 440
BspACI CCGC 3 cut(s) 71, 74, 323
BspANI GGCC 5 cut(s) 116, 210, 326, 338, 347
BspLI GGNNCC 2 cut(s) 161, 257
BspPI GGATC 1 cut(s) 208
BspT107I GGYRCC 1 cut(s) 255
BsrBI CCGCTC 1 cut(s) 71
BssECI CCNNGG 4 cut(s) 5, 198, 348, 440
BssMI GATC 1 cut(s) 213
BssT1I CCWWGG 3 cut(s) 198, 348, 440
Bst2UI CCWGG 1 cut(s) 335
BstDEI CTNAG 1 cut(s) 136
BstDSI CCRYGG 3 cut(s) 198, 348, 440
BstF5I GGATG 2 cut(s) 418, 448
BstKTI GATC 1 cut(s) 216
BstMAI GTCTC 1 cut(s) 347
BstMBI GATC 1 cut(s) 213
BstMWI GCNNNNNNNGC 3 cut(s) 113, 344, 445
BstNI CCWGG 1 cut(s) 335
BstSCI CCNGG 2 cut(s) 5, 333
BstSFI CTRYAG 1 cut(s) 96
BstV1I GCAGC 4 cut(s) 91, 152, 255, 388
BstX2I RGATCY 1 cut(s) 213
BstXI CCANNNNNNTGG 1 cut(s) 334
BstYI RGATCY 1 cut(s) 213
BsuRI GGCC 5 cut(s) 116, 210, 326, 338, 347
BtgI CCRYGG 3 cut(s) 198, 348, 440
BtsCI GGATG 2 cut(s) 418, 448
CaiI CAGNNNCTG 1 cut(s) 104
CviAII CATG 4 cut(s) 199, 299, 349, 441
DdeI CTNAG 1 cut(s) 136
DpnI GATC 1 cut(s) 215
DpnII GATC 1 cut(s) 213
DraI TTTAAA 1 cut(s) 52
EaeI YGGCCR 3 cut(s) 324, 336, 345
Eco130I CCWWGG 3 cut(s) 198, 348, 440
Eco57I CTGAAG 1 cut(s) 414
EcoRII CCWGG 1 cut(s) 333
EcoT14I CCWWGG 3 cut(s) 198, 348, 440
ErhI CCWWGG 3 cut(s) 198, 348, 440
Esp3I CGTCTC 1 cut(s) 347
FaeI CATG 4 cut(s) 202, 302, 352, 444
FaiI YATR 9 cut(s) 12, 20, 28, 30, 200, 300, 350, 375, 442
FatI CATG 4 cut(s) 198, 298, 348, 440
FauI CCCGC 1 cut(s) 67
Fnu4HI GCNGC 6 cut(s) 72, 105, 141, 269, 324, 402
FokI GGATG 2 cut(s) 425, 435
Fsp4HI GCNGC 6 cut(s) 72, 105, 141, 269, 324, 402
FspBI CTAG 2 cut(s) 42, 453
GluI GCNGC 6 cut(s) 72, 105, 141, 269, 324, 402
HaeIII GGCC 5 cut(s) 116, 210, 326, 338, 347
HapII CCGG 1 cut(s) 7
Hin1II CATG 4 cut(s) 202, 302, 352, 444
HincII GTYRAC 1 cut(s) 93
HindII GTYRAC 1 cut(s) 93
HpaII CCGG 1 cut(s) 7
Hpy166II GTNNAC 1 cut(s) 93
Hpy188I TCNGA 2 cut(s) 226, 281
Hpy188III TCNNGA 2 cut(s) 80, 410
Hpy8I GTNNAC 1 cut(s) 93
HpyAV CCTTC 3 cut(s) 167, 202, 255
HpyCH4V TGCA 4 cut(s) 107, 377, 404, 416
HpyF10VI GCNNNNNNNGC 3 cut(s) 113, 344, 445
HpyF3I CTNAG 1 cut(s) 136
Hsp92II CATG 4 cut(s) 202, 302, 352, 444
Kzo9I GATC 1 cut(s) 213
LmnI GCTCC 4 cut(s) 68, 241, 265, 445
Lsp1109I GCAGC 4 cut(s) 91, 152, 255, 388
LweI GCATC 3 cut(s) 91, 403, 457
MaeI CTAG 2 cut(s) 42, 453
MaeIII GTNAC 2 cut(s) 34, 202
MalI GATC 1 cut(s) 215
MbiI CCGCTC 1 cut(s) 71
MboI GATC 1 cut(s) 213
MflI RGATCY 1 cut(s) 213
MlsI TGGCCA 2 cut(s) 338, 347
MluCI AATT 2 cut(s) 274, 405
MluNI TGGCCA 2 cut(s) 338, 347
MnlI CCTC 7 cut(s) 76, 149, 173, 228, 313, 352, 396
Mox20I TGGCCA 2 cut(s) 338, 347
MscI TGGCCA 2 cut(s) 338, 347
MseI TTAA 2 cut(s) 23, 51
Msp20I TGGCCA 2 cut(s) 338, 347
MspI CCGG 1 cut(s) 7
MspR9I CCNGG 2 cut(s) 7, 335
MvaI CCWGG 1 cut(s) 335
MwoI GCNNNNNNNGC 3 cut(s) 113, 344, 445
NciI CCSGG 1 cut(s) 7
NcoI CCATGG 3 cut(s) 198, 348, 440
NdeII GATC 1 cut(s) 213
NlaIII CATG 4 cut(s) 202, 302, 352, 444
NlaIV GGNNCC 2 cut(s) 161, 257
NmuCI GTSAC 1 cut(s) 202
PkrI GCNGC 6 cut(s) 73, 106, 142, 270, 325, 403
PshBI ATTAAT 1 cut(s) 23
Psp6I CCWGG 1 cut(s) 333
PspGI CCWGG 1 cut(s) 333
PspN4I GGNNCC 2 cut(s) 161, 257
PstNI CAGNNNCTG 1 cut(s) 104
PsuI RGATCY 1 cut(s) 213
SaqAI TTAA 2 cut(s) 23, 51
SatI GCNGC 6 cut(s) 72, 105, 141, 269, 324, 402
Sau3AI GATC 1 cut(s) 213
ScrFI CCNGG 2 cut(s) 7, 335
SfaNI GCATC 3 cut(s) 91, 403, 457
SfcI CTRYAG 1 cut(s) 96
SfiI GGCCNNNNNGGCC 1 cut(s) 344
Sse9I AATT 2 cut(s) 274, 405
SsiI CCGC 3 cut(s) 71, 74, 323
SspMI CTAG 2 cut(s) 42, 453
StyD4I CCNGG 2 cut(s) 5, 333
StyI CCWWGG 3 cut(s) 198, 348, 440
TaqI TCGA 1 cut(s) 165
TasI AATT 2 cut(s) 274, 405
TauI GCSGC 2 cut(s) 74, 326
Tru1I TTAA 2 cut(s) 23, 51
Tru9I TTAA 2 cut(s) 23, 51
TseFI GTSAC 1 cut(s) 202
TseI GCWGC 4 cut(s) 104, 140, 268, 401
Tsp45I GTSAC 1 cut(s) 202
TspDTI ATGAA 3 cut(s) 45, 198, 243
TspGWI ACGGA 1 cut(s) 371
VspI ATTAAT 1 cut(s) 23
XapI RAATTY 1 cut(s) 274
XcmI CCANNNNNNNNNTGG 1 cut(s) 346
XspI CTAG 2 cut(s) 42, 453
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.