RchiOBHm_Chr1g0349671

Plant invertase/pectin methylesterase inhibitor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
1
OG0017953
Physical Location & Seq
Reverse (-)
42762618 .. 42763259
642 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 642 bp
ATGGCCAAACTTTGCCTTTCTTTGCTGCTAATCCTCCTTTCCATCTTCTCCCTTTCGAGCACGGTGGAGCCAGCCTTTTCCGGACATACTGCTCGACGTTCCAGGGCTCGAGCCAACATCGAAGCCGCATGCCGGACCACCCGCTACCCGGACCTATGCGTCCACTGCCTCACCACCTACGTCAAACCCAACATGACCATCCAAAGCCCCCAGCAACTTGCCCAAGTCGCCTTGACTGCAAGCCTCTACCGTGCCCTATATGCCAGGGCGTTCGTGCTCAAGGTAGCAAAAGAGCTTAACACACTGAAGAGGGAAAACCAAGTTGTCCGAGATTGCTTGCAGCAGATAAACGATAGCGTGGACCAACTCAGTGGATCGATCAAGGAACTACGTCGTTTGGGGCAAGAATCTGTGGGTGATGACCTGTTTTGGCACATAAGCAACGTTGAGACATGGGTTGGCACTGCTCTAACTGATGCAAACGATTGTGTAAATGCCCTTCCGGGACCAAACATGAGCAAACTGAAGGCGACTATTAAAGGAAAGATGCTCAATGTGGCTCAGACGGCTAGTAATGCACTGGCTTTGTTTCATAGTTTTGCTGCAAGGTATAAAGCTGCCAGTGCCACCAAGAAGCCCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PMEI

Protein Analysis

213

Amino Acids

23.38

Weight (kDa)

9.6

Isoelectric Point (pI)

41.93

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PMEI PF04043 39 - 196 1.9e-32 Plant invertase/pectin methylesterase inhibitor
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0017953)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G00080
fragaria_vesca FvH4_7g11390
prunus_persica Prupe.2G141800_v2.0.a1
rosa_chinensis RchiOBHm_Chr1g0349671
rosa_laevigata RLG00000028536
rosa_roxburghii Rroxscaffold_4G00305390
rosa_rugosa Rorug01G0204900
rosa_samantha Rh1AG222500 Rh1BG188500 Rh1DG217700
rosa_wichuraiana Rw1G018970

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 158
AccIII TCCGGA 1 cut(s) 80
AciI CCGC 2 cut(s) 126, 142
AclI AACGTT 1 cut(s) 444
AclWI GGATC 1 cut(s) 382
AcoI YGGCCR 1 cut(s) 3
AcuI CTGAAG 2 cut(s) 326, 545
AfiI CCNNNNNNNGG 2 cut(s) 132, 148
AjnI CCWGG 2 cut(s) 101, 263
AluBI AGCT 2 cut(s) 295, 617
AluI AGCT 2 cut(s) 295, 617
Alw21I GWGCWC 2 cut(s) 62, 279
Alw26I GTCTC 1 cut(s) 443
AlwI GGATC 1 cut(s) 382
Ama87I CYCGRG 1 cut(s) 108
Aor13HI TCCGGA 1 cut(s) 80
AoxI GGCC 1 cut(s) 3
ApeKI GCWGC 4 cut(s) 25, 340, 602, 617
AspS9I GGNCC 4 cut(s) 135, 151, 361, 506
AsuC2I CCSGG 2 cut(s) 149, 504
AsuHPI GGTGA 2 cut(s) 163, 428
AvaI CYCGRG 1 cut(s) 108
AvaII GGWCC 4 cut(s) 135, 151, 361, 506
BaeGI GKGCMC 1 cut(s) 256
BalI TGGCCA 1 cut(s) 5
BanII GRGCYC 1 cut(s) 109
BarI GAAGNNNNNNTAC 2 cut(s) 483, 515
Bbv12I GWGCWC 2 cut(s) 62, 279
BbvI GCAGC 4 cut(s) 12, 352, 589, 604
BccI CCATC 2 cut(s) 50, 206
BceAI ACGGC 1 cut(s) 582
BciT130I CCWGG 2 cut(s) 103, 265
BcnI CCSGG 2 cut(s) 149, 504
BcoDI GTCTC 1 cut(s) 443
BfaI CTAG 2 cut(s) 570, 640
BisI GCNGC 5 cut(s) 26, 126, 341, 603, 618
BlsI GCNGC 5 cut(s) 27, 127, 342, 604, 619
Bme1390I CCNGG 4 cut(s) 103, 149, 265, 504
Bme18I GGWCC 4 cut(s) 135, 151, 361, 506
BmeT110I CYCGRG 1 cut(s) 108
BmgT120I GGNCC 4 cut(s) 135, 151, 361, 506
BmiI GGNNCC 2 cut(s) 69, 507
BmrFI CCNGG 4 cut(s) 103, 149, 265, 504
BmsI GCATC 2 cut(s) 466, 537
BpuEI CTTGAG 1 cut(s) 263
BpuMI CCSGG 2 cut(s) 149, 504
Bsa29I ATCGAT 1 cut(s) 377
BsaJI CCNNGG 2 cut(s) 102, 264
BsaWI WCCGGW 1 cut(s) 80
Bsc4I CCNNNNNNNGG 2 cut(s) 132, 148
Bse1I ACTGG 2 cut(s) 585, 621
BseAI TCCGGA 1 cut(s) 80
BseBI CCWGG 2 cut(s) 103, 265
BseCI ATCGAT 1 cut(s) 377
BseDI CCNNGG 2 cut(s) 102, 264
BseGI GGATG 1 cut(s) 198
BseLI CCNNNNNNNGG 2 cut(s) 132, 148
BseMII CTCAG 2 cut(s) 382, 575
BseNI ACTGG 2 cut(s) 585, 621
BseSI GKGCMC 1 cut(s) 256
BseXI GCAGC 4 cut(s) 12, 352, 589, 604
BseYI CCCAGC 1 cut(s) 210
BshFI GGCC 1 cut(s) 5
BshVI ATCGAT 1 cut(s) 377
BsiHKAI GWGCWC 2 cut(s) 62, 279
BsiHKCI CYCGRG 1 cut(s) 108
BsiSI CCGG 4 cut(s) 81, 133, 149, 503
BslFI GGGAC 1 cut(s) 519
BslI CCNNNNNNNGG 2 cut(s) 132, 148
BsmAI GTCTC 1 cut(s) 443
BsmFI GGGAC 1 cut(s) 519
BsnI GGCC 1 cut(s) 5
BsoBI CYCGRG 1 cut(s) 108
Bsp1286I GDGCHC 4 cut(s) 62, 109, 256, 279
Bsp13I TCCGGA 1 cut(s) 80
Bsp143I GATC 2 cut(s) 374, 378
BspACI CCGC 2 cut(s) 126, 142
BspANI GGCC 1 cut(s) 5
BspCNI CTCAG 2 cut(s) 381, 574
BspDI ATCGAT 1 cut(s) 377
BspEI TCCGGA 1 cut(s) 80
BspLI GGNNCC 2 cut(s) 69, 507
BspPI GGATC 1 cut(s) 382
BsrI ACTGG 2 cut(s) 585, 621
BssECI CCNNGG 2 cut(s) 102, 264
BssMI GATC 2 cut(s) 374, 378
Bst2UI CCWGG 2 cut(s) 103, 265
Bst4CI ACNGT 2 cut(s) 64, 251
Bst6I CTCTTC 1 cut(s) 302
BstC8I GCNNGC 4 cut(s) 72, 130, 241, 338
BstDEI CTNAG 2 cut(s) 368, 561
BstF5I GGATG 1 cut(s) 198
BstKTI GATC 2 cut(s) 377, 381
BstMAI GTCTC 1 cut(s) 443
BstMBI GATC 2 cut(s) 374, 378
BstMWI GCNNNNNNNGC 7 cut(s) 165, 227, 236, 260, 566, 575, 623
BstNI CCWGG 2 cut(s) 103, 265
BstNSI RCATGY 1 cut(s) 132
BstSCI CCNGG 4 cut(s) 101, 147, 263, 502
BstSLI GKGCMC 1 cut(s) 256
BstV1I GCAGC 4 cut(s) 12, 352, 589, 604
BstXI CCANNNNNNTGG 1 cut(s) 371
Bsu15I ATCGAT 1 cut(s) 377
BsuRI GGCC 1 cut(s) 5
BsuTUI ATCGAT 1 cut(s) 377
BtsCI GGATG 1 cut(s) 198
BtsI GCAGTG 2 cut(s) 163, 462
BtsIMutI CAGTG 6 cut(s) 163, 302, 376, 462, 578, 628
Cac8I GCNNGC 4 cut(s) 72, 130, 241, 338
Cfr13I GGNCC 4 cut(s) 135, 151, 361, 506
ClaI ATCGAT 1 cut(s) 377
CseI GACGC 1 cut(s) 148
CviAII CATG 4 cut(s) 129, 193, 453, 514
DdeI CTNAG 2 cut(s) 368, 561
DpnI GATC 2 cut(s) 376, 380
DpnII GATC 2 cut(s) 374, 378
DrdI GACNNNNNNGTC 1 cut(s) 158
DseDI GACNNNNNNGTC 1 cut(s) 158
EaeI YGGCCR 1 cut(s) 3
Eam1104I CTCTTC 1 cut(s) 302
EarI CTCTTC 1 cut(s) 302
Eco24I GRGCYC 1 cut(s) 109
Eco47I GGWCC 4 cut(s) 135, 151, 361, 506
Eco57I CTGAAG 2 cut(s) 326, 545
Eco88I CYCGRG 1 cut(s) 108
EcoRII CCWGG 2 cut(s) 101, 263
EcoT38I GRGCYC 1 cut(s) 109
FaeI CATG 4 cut(s) 132, 196, 456, 517
FaqI GGGAC 1 cut(s) 519
FatI CATG 4 cut(s) 128, 192, 452, 513
FauI CCCGC 1 cut(s) 149
Fnu4HI GCNGC 5 cut(s) 26, 126, 341, 603, 618
FokI GGATG 1 cut(s) 185
FriOI GRGCYC 1 cut(s) 109
Fsp4HI GCNGC 5 cut(s) 26, 126, 341, 603, 618
FspBI CTAG 2 cut(s) 570, 640
GluI GCNGC 5 cut(s) 26, 126, 341, 603, 618
GsaI CCCAGC 1 cut(s) 214
HaeIII GGCC 1 cut(s) 5
HapII CCGG 4 cut(s) 81, 133, 149, 503
HgaI GACGC 1 cut(s) 148
Hin1II CATG 4 cut(s) 132, 196, 456, 517
HinfI GANTC 1 cut(s) 407
HpaII CCGG 4 cut(s) 81, 133, 149, 503
HphI GGTGA 2 cut(s) 163, 428
Hpy166II GTNNAC 2 cut(s) 163, 361
Hpy188I TCNGA 2 cut(s) 329, 564
Hpy188III TCNNGA 1 cut(s) 81
Hpy8I GTNNAC 2 cut(s) 163, 361
Hpy99I CGWCG 2 cut(s) 99, 396
HpyAV CCTTC 2 cut(s) 509, 520
HpyCH4III ACNGT 2 cut(s) 64, 251
HpyCH4IV ACGT 4 cut(s) 97, 180, 391, 444
HpyCH4V TGCA 5 cut(s) 239, 340, 479, 578, 605
HpyF10VI GCNNNNNNNGC 7 cut(s) 165, 227, 236, 260, 566, 575, 623
HpyF3I CTNAG 2 cut(s) 368, 561
HpySE526I ACGT 4 cut(s) 97, 180, 391, 444
Hsp92II CATG 4 cut(s) 132, 196, 456, 517
Kpn2I TCCGGA 1 cut(s) 80
Kzo9I GATC 2 cut(s) 374, 378
LmnI GCTCC 1 cut(s) 67
Lsp1109I GCAGC 4 cut(s) 12, 352, 589, 604
LweI GCATC 2 cut(s) 466, 537
MaeI CTAG 2 cut(s) 570, 640
MaeII ACGT 4 cut(s) 97, 180, 391, 444
MalI GATC 2 cut(s) 376, 380
MboI GATC 2 cut(s) 374, 378
MboII GAAGA 2 cut(s) 37, 319
MhlI GDGCHC 4 cut(s) 62, 109, 256, 279
MlsI TGGCCA 1 cut(s) 5
MluNI TGGCCA 1 cut(s) 5
MnlI CCTC 4 cut(s) 44, 179, 254, 303
Mox20I TGGCCA 1 cut(s) 5
MroI TCCGGA 1 cut(s) 80
MscI TGGCCA 1 cut(s) 5
MseI TTAA 2 cut(s) 297, 537
Msp20I TGGCCA 1 cut(s) 5
MspI CCGG 4 cut(s) 81, 133, 149, 503
MspR9I CCNGG 4 cut(s) 103, 149, 265, 504
MvaI CCWGG 2 cut(s) 103, 265
MwoI GCNNNNNNNGC 7 cut(s) 165, 227, 236, 260, 566, 575, 623
NciI CCSGG 2 cut(s) 149, 504
NdeII GATC 2 cut(s) 374, 378
NlaIII CATG 4 cut(s) 132, 196, 456, 517
NlaIV GGNNCC 2 cut(s) 69, 507
NspI RCATGY 1 cut(s) 132
PaeI GCATGC 1 cut(s) 132
PaeR7I CTCGAG 1 cut(s) 108
PfeI GAWTC 1 cut(s) 407
PfoI TCCNGGA 1 cut(s) 502
PkrI GCNGC 5 cut(s) 27, 127, 342, 604, 619
Psp1406I AACGTT 1 cut(s) 444
Psp6I CCWGG 2 cut(s) 101, 263
PspFI CCCAGC 1 cut(s) 210
PspGI CCWGG 2 cut(s) 101, 263
PspN4I GGNNCC 2 cut(s) 69, 507
PspPI GGNCC 4 cut(s) 135, 151, 361, 506
PspXI VCTCGAGB 1 cut(s) 108
SaqAI TTAA 2 cut(s) 297, 537
SatI GCNGC 5 cut(s) 26, 126, 341, 603, 618
Sau3AI GATC 2 cut(s) 374, 378
Sau96I GGNCC 4 cut(s) 135, 151, 361, 506
ScrFI CCNGG 4 cut(s) 103, 149, 265, 504
SduI GDGCHC 4 cut(s) 62, 109, 256, 279
SfaNI GCATC 2 cut(s) 466, 537
Sfr274I CTCGAG 1 cut(s) 108
SinI GGWCC 4 cut(s) 135, 151, 361, 506
SlaI CTCGAG 1 cut(s) 108
SmlI CTYRAG 2 cut(s) 108, 278
SmoI CTYRAG 2 cut(s) 108, 278
SphI GCATGC 1 cut(s) 132
SsiI CCGC 2 cut(s) 126, 142
SspMI CTAG 2 cut(s) 570, 640
StyD4I CCNGG 4 cut(s) 101, 147, 263, 502
TaaI ACNGT 2 cut(s) 64, 251
TaiI ACGT 4 cut(s) 100, 183, 394, 447
TaqI TCGA 5 cut(s) 56, 94, 109, 120, 377
TauI GCSGC 1 cut(s) 128
TfiI GAWTC 1 cut(s) 407
Tru1I TTAA 2 cut(s) 297, 537
Tru9I TTAA 2 cut(s) 297, 537
TscAI CASTG 6 cut(s) 170, 309, 376, 469, 585, 628
TseI GCWGC 4 cut(s) 25, 340, 602, 617
TspDTI ATGAA 1 cut(s) 581
TspRI CASTG 6 cut(s) 170, 309, 376, 469, 585, 628
VpaK11BI GGWCC 4 cut(s) 135, 151, 361, 506
XceI RCATGY 1 cut(s) 132
XhoI CTCGAG 1 cut(s) 108
XspI CTAG 2 cut(s) 570, 640
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.