RCHIOBHM_CPG0501931

cytochrome b6

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
Pt
OG0019578
Physical Location & Seq
Reverse (-)
35419 .. 35912
494 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ15684

Sequence Viewer

Length: 297 bp
ATGACTTTTTATTATCGTCCGACCGTTACAGAGGCTTTTGCTTCTGTTCAATATATAATGACTGAAGTTAACTTTGGTTGGTTAATCCGATCAGTTCATCGATGGTCGGCAAGTATGATGGTCCTAATGATGATTCTGCACGTATTTCGTGTGTATCTCACCGGTGGTTTTAAAAAACCTCGTGAATTGACTTGGGTTACAGGTGTGGTTTTGGGTGTATTAACCGCATCTTTTGGTGTAACTGGTTATTCCTTACCTTGGGACCAAATTGGTTATTGGGCAGATCGCCCCTGGTAG

Protein Analysis

98

Amino Acids

11.42

Weight (kDa)

9.52

Isoelectric Point (pI)

18.45

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Cytochrome_B PF00033 1 - 94 5.3e-44 Cytochrome b/b6/petB
Cytochrom_B_N_2 PF13631 39 - 94 1.6e-19 Cytochrome b(N-terminal)/b6/petB
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0019578)

Species Orthologous Gene IDs
arabidopsis_thaliana ATCG00730
malus_domestica MD16G1017300.v1.1
pyrus_communis pycom12397g00180
rosa_chinensis RchiOBHm_Chr3g0476911 RchiOBHm_CPg0501911 RchiOBHm_CPg0501931
rosa_wichuraiana Rw7G013570

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 225
AcuI CTGAAG 1 cut(s) 84
AfiI CCNNNNNNNGG 1 cut(s) 258
AgeI ACCGGT 1 cut(s) 161
AgsI TTSAA 1 cut(s) 50
AjnI CCWGG 1 cut(s) 290
AjuI GAANNNNNNNTTGG 2 cut(s) 57, 89
AsiGI ACCGGT 1 cut(s) 161
AspS9I GGNCC 2 cut(s) 121, 262
AsuHPI GGTGA 1 cut(s) 151
AvaII GGWCC 2 cut(s) 121, 262
BauI CACGAG 1 cut(s) 180
BccI CCATC 2 cut(s) 96, 112
BcgI CGANNNNNNTGC 2 cut(s) 128, 162
BciT130I CCWGG 1 cut(s) 292
Bme1390I CCNGG 1 cut(s) 292
Bme18I GGWCC 2 cut(s) 121, 262
BmgT120I GGNCC 2 cut(s) 121, 262
BmiI GGNNCC 1 cut(s) 263
BmrFI CCNGG 1 cut(s) 292
BmsI GCATC 1 cut(s) 236
Bsa29I ATCGAT 1 cut(s) 100
BsaAI YACGTR 1 cut(s) 142
BsaJI CCNNGG 2 cut(s) 257, 290
BsaWI WCCGGW 1 cut(s) 161
Bsc4I CCNNNNNNNGG 1 cut(s) 258
Bse118I RCCGGY 1 cut(s) 161
Bse1I ACTGG 1 cut(s) 247
BseBI CCWGG 1 cut(s) 292
BseCI ATCGAT 1 cut(s) 100
BseDI CCNNGG 2 cut(s) 257, 290
BseLI CCNNNNNNNGG 1 cut(s) 258
BseNI ACTGG 1 cut(s) 247
BsgI GTGCAG 1 cut(s) 122
Bsh1285I CGRYCG 1 cut(s) 24
BshTI ACCGGT 1 cut(s) 161
BshVI ATCGAT 1 cut(s) 100
BsiEI CGRYCG 1 cut(s) 24
BsiSI CCGG 1 cut(s) 162
BslFI GGGAC 1 cut(s) 275
BslI CCNNNNNNNGG 1 cut(s) 258
BsmFI GGGAC 1 cut(s) 275
Bsp143I GATC 2 cut(s) 89, 283
BspACI CCGC 1 cut(s) 225
BspDI ATCGAT 1 cut(s) 100
BspLI GGNNCC 1 cut(s) 263
BsrFI RCCGGY 1 cut(s) 161
BsrI ACTGG 1 cut(s) 247
BssAI RCCGGY 1 cut(s) 161
BssECI CCNNGG 2 cut(s) 257, 290
BssMI GATC 2 cut(s) 89, 283
BssSI CACGAG 1 cut(s) 180
BssT1I CCWWGG 1 cut(s) 257
Bst2BI CACGAG 1 cut(s) 180
Bst2UI CCWGG 1 cut(s) 292
Bst4CI ACNGT 1 cut(s) 25
BstBAI YACGTR 1 cut(s) 142
BstKTI GATC 2 cut(s) 92, 286
BstMBI GATC 2 cut(s) 89, 283
BstMCI CGRYCG 1 cut(s) 24
BstNI CCWGG 1 cut(s) 292
BstSCI CCNGG 1 cut(s) 290
Bsu15I ATCGAT 1 cut(s) 100
BsuTUI ATCGAT 1 cut(s) 100
Cfr10I RCCGGY 1 cut(s) 161
Cfr13I GGNCC 2 cut(s) 121, 262
ClaI ATCGAT 1 cut(s) 100
CspAI ACCGGT 1 cut(s) 161
CviJI RGCY 1 cut(s) 35
CviKI_1 RGCY 1 cut(s) 35
DpnI GATC 2 cut(s) 91, 285
DpnII GATC 2 cut(s) 89, 283
DraI TTTAAA 1 cut(s) 172
Eco130I CCWWGG 1 cut(s) 257
Eco47I GGWCC 2 cut(s) 121, 262
Eco57I CTGAAG 1 cut(s) 84
EcoRII CCWGG 1 cut(s) 290
EcoT14I CCWWGG 1 cut(s) 257
ErhI CCWWGG 1 cut(s) 257
FaiI YATR 3 cut(s) 54, 56, 116
FaqI GGGAC 1 cut(s) 275
HapII CCGG 1 cut(s) 162
HincII GTYRAC 1 cut(s) 70
HindII GTYRAC 1 cut(s) 70
HinfI GANTC 1 cut(s) 133
HpaI GTTAAC 1 cut(s) 70
HpaII CCGG 1 cut(s) 162
HphI GGTGA 1 cut(s) 151
Hpy166II GTNNAC 1 cut(s) 70
Hpy188I TCNGA 2 cut(s) 21, 89
Hpy188III TCNNGA 1 cut(s) 182
Hpy8I GTNNAC 1 cut(s) 70
HpyCH4III ACNGT 1 cut(s) 25
HpyCH4IV ACGT 1 cut(s) 141
HpyCH4V TGCA 1 cut(s) 139
HpySE526I ACGT 1 cut(s) 141
KspAI GTTAAC 1 cut(s) 70
Kzo9I GATC 2 cut(s) 89, 283
LpnPI CCDG 4 cut(s) 175, 186, 228, 277
LweI GCATC 1 cut(s) 236
MaeII ACGT 1 cut(s) 141
MaeIII GTNAC 3 cut(s) 25, 196, 238
MalI GATC 2 cut(s) 91, 285
MboI GATC 2 cut(s) 89, 283
MluCI AATT 2 cut(s) 185, 267
MmeI TCCRAC 1 cut(s) 44
MnlI CCTC 2 cut(s) 25, 189
MseI TTAA 4 cut(s) 69, 83, 171, 221
MspI CCGG 1 cut(s) 162
MspR9I CCNGG 1 cut(s) 292
MvaI CCWGG 1 cut(s) 292
NdeII GATC 2 cut(s) 89, 283
NlaIV GGNNCC 1 cut(s) 263
PfeI GAWTC 1 cut(s) 133
PinAI ACCGGT 1 cut(s) 161
Ppu21I YACGTR 1 cut(s) 142
Psp6I CCWGG 1 cut(s) 290
PspGI CCWGG 1 cut(s) 290
PspN4I GGNNCC 1 cut(s) 263
PspPI GGNCC 2 cut(s) 121, 262
SaqAI TTAA 4 cut(s) 69, 83, 171, 221
Sau3AI GATC 2 cut(s) 89, 283
Sau96I GGNCC 2 cut(s) 121, 262
ScrFI CCNGG 1 cut(s) 292
SetI ASST 4 cut(s) 144, 181, 205, 259
SfaNI GCATC 1 cut(s) 236
SgrAI CRCCGGYG 1 cut(s) 161
SinI GGWCC 2 cut(s) 121, 262
Sse9I AATT 2 cut(s) 185, 267
SsiI CCGC 1 cut(s) 225
StyD4I CCNGG 1 cut(s) 290
StyI CCWWGG 1 cut(s) 257
TaaI ACNGT 1 cut(s) 25
TaiI ACGT 1 cut(s) 144
TaqI TCGA 1 cut(s) 100
TasI AATT 2 cut(s) 185, 267
TfiI GAWTC 1 cut(s) 133
Tru1I TTAA 4 cut(s) 69, 83, 171, 221
Tru9I TTAA 4 cut(s) 69, 83, 171, 221
TspDTI ATGAA 1 cut(s) 86
VpaK11BI GGWCC 2 cut(s) 121, 262
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.