Rw7G013570

Cytochrome b6-f complex subunit 4

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr7
OG0019578
Physical Location & Seq
Reverse (-)
13033570 .. 13034772
1203 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw7G013570.1

Sequence Viewer

Length: 501 bp
ATGGGAAATAATTCACCTATCCTAATAACAAAAAAACCTGACTTGAACGATCCTGTGTTAAGAGCTAAATTGGCTAAAGGGATGGGTCATAATTATTATGGAGAACCCGCATGGCCAAATGATCTTTTATATATTTTTCCAGTAGTAATTTTAGGTACTATTGCATGTAACGTAGGCTTAGCTGTTTTAGAACCATCAATGATTGGTGAACCCGCGGATCCATTTGCAACTCCTTTGGAAATATTGCCTGAATGGTATTTCTTTCCCGTATTTCAAATACTTCGTACAGTGCCAAATAAGTTATTGGGTGTTCTTTTAATGGTTTCAGTACCCGCGGGATTATTAACAGTACCTTTTTTGGAGAATGTTAATAAATTCCAAAATCCATTTCGTCGTCCCGTAGCGACAACCGTTTTTTTGATTGGTACTGCAGTAGCTCTTTGGTTGGGTATTGGAGCAACACTACCTATTGATAAGTCCCTAACTTTAGGTCTTTTTTAA

Protein Analysis

166

Amino Acids

18.11

Weight (kDa)

6.56

Isoelectric Point (pI)

51.54

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Cytochrom_B_C PF00032 71 - 160 1.8e-19 Cytochrome b(C-terminal)/b6/petD
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0019578)

Species Orthologous Gene IDs
arabidopsis_thaliana ATCG00730
malus_domestica MD16G1017300.v1.1
pyrus_communis pycom12397g00180
rosa_chinensis RchiOBHm_Chr3g0476911 RchiOBHm_CPg0501911 RchiOBHm_CPg0501931
rosa_wichuraiana Rw7G013570

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 2 cut(s) 215, 335
AciI CCGC 5 cut(s) 108, 213, 215, 333, 335
AclWI GGATC 3 cut(s) 44, 212, 225
AcoI YGGCCR 1 cut(s) 113
AcsI RAATTY 1 cut(s) 374
AfaI GTAC 5 cut(s) 157, 286, 330, 351, 427
AgsI TTSAA 2 cut(s) 46, 275
AjuI GAANNNNNNNTTGG 2 cut(s) 372, 404
AluBI AGCT 3 cut(s) 65, 182, 437
AluI AGCT 3 cut(s) 65, 182, 437
AlwI GGATC 3 cut(s) 44, 212, 225
AoxI GGCC 1 cut(s) 113
ApoI RAATTY 1 cut(s) 374
Asp700I GAANNNNTTC 1 cut(s) 10
AsuHPI GGTGA 2 cut(s) 6, 218
BaeI ACNNNNGTAYC 2 cut(s) 417, 450
BalI TGGCCA 1 cut(s) 115
BamHI GGATCC 1 cut(s) 217
BccI CCATC 2 cut(s) 76, 202
BfmI CTRYAG 1 cut(s) 429
BlpI GCTNAGC 1 cut(s) 178
BmiI GGNNCC 1 cut(s) 219
Bpu1102I GCTNAGC 1 cut(s) 178
BsaJI CCNNGG 2 cut(s) 213, 333
Bse1I ACTGG 1 cut(s) 140
BseDI CCNNGG 2 cut(s) 213, 333
BseGI GGATG 1 cut(s) 87
BseNI ACTGG 1 cut(s) 140
Bsh1236I CGCG 2 cut(s) 215, 335
BshFI GGCC 1 cut(s) 115
BslFI GGGAC 2 cut(s) 381, 463
BsmFI GGGAC 2 cut(s) 381, 463
BsnI GGCC 1 cut(s) 115
Bsp143I GATC 3 cut(s) 49, 121, 217
Bsp1720I GCTNAGC 1 cut(s) 178
BspACI CCGC 5 cut(s) 108, 213, 215, 333, 335
BspANI GGCC 1 cut(s) 115
BspFNI CGCG 2 cut(s) 215, 335
BspLI GGNNCC 1 cut(s) 219
BspMAI CTGCAG 1 cut(s) 433
BspPI GGATC 3 cut(s) 44, 212, 225
BsrI ACTGG 1 cut(s) 140
BssECI CCNNGG 2 cut(s) 213, 333
BssMI GATC 3 cut(s) 49, 121, 217
Bst4CI ACNGT 3 cut(s) 289, 349, 412
BstDEI CTNAG 1 cut(s) 178
BstDSI CCRYGG 2 cut(s) 213, 333
BstF5I GGATG 1 cut(s) 87
BstFNI CGCG 2 cut(s) 215, 335
BstKTI GATC 3 cut(s) 52, 124, 220
BstMBI GATC 3 cut(s) 49, 121, 217
BstMWI GCNNNNNNNGC 1 cut(s) 71
BstNSI RCATGY 1 cut(s) 168
BstSFI CTRYAG 1 cut(s) 429
BstUI CGCG 2 cut(s) 215, 335
BstX2I RGATCY 1 cut(s) 217
BstYI RGATCY 1 cut(s) 217
BsuRI GGCC 1 cut(s) 115
BtgI CCRYGG 2 cut(s) 213, 333
BtsCI GGATG 1 cut(s) 87
BtsIMutI CAGTG 1 cut(s) 294
Cfr42I CCGCGG 2 cut(s) 216, 336
Csp6I GTAC 5 cut(s) 156, 285, 329, 350, 426
CviAII CATG 2 cut(s) 111, 165
CviJI RGCY 6 cut(s) 65, 74, 115, 177, 182, 437
CviKI_1 RGCY 6 cut(s) 65, 74, 115, 177, 182, 437
CviQI GTAC 5 cut(s) 156, 285, 329, 350, 426
DdeI CTNAG 1 cut(s) 178
DpnI GATC 3 cut(s) 51, 123, 219
DpnII GATC 3 cut(s) 49, 121, 217
EaeI YGGCCR 1 cut(s) 113
FaeI CATG 2 cut(s) 114, 168
FaiI YATR 6 cut(s) 90, 99, 112, 130, 132, 166
FaqI GGGAC 2 cut(s) 381, 463
FatI CATG 2 cut(s) 110, 164
FauI CCCGC 4 cut(s) 115, 220, 328, 340
FokI GGATG 1 cut(s) 94
HaeIII GGCC 1 cut(s) 115
Hin1II CATG 2 cut(s) 114, 168
HphI GGTGA 2 cut(s) 6, 218
Hpy166II GTNNAC 1 cut(s) 209
Hpy8I GTNNAC 1 cut(s) 209
Hpy99I CGWCG 1 cut(s) 396
HpyCH4III ACNGT 3 cut(s) 289, 349, 412
HpyCH4IV ACGT 1 cut(s) 171
HpyCH4V TGCA 3 cut(s) 164, 227, 431
HpyF10VI GCNNNNNNNGC 1 cut(s) 71
HpyF3I CTNAG 1 cut(s) 178
HpySE526I ACGT 1 cut(s) 171
Hsp92II CATG 2 cut(s) 114, 168
KspI CCGCGG 2 cut(s) 216, 336
Kzo9I GATC 3 cut(s) 49, 121, 217
LmnI GCTCC 1 cut(s) 455
LpnPI CCDG 4 cut(s) 51, 66, 153, 261
MaeII ACGT 1 cut(s) 171
MaeIII GTNAC 1 cut(s) 167
MalI GATC 3 cut(s) 51, 123, 219
MboI GATC 3 cut(s) 49, 121, 217
MflI RGATCY 1 cut(s) 217
MlsI TGGCCA 1 cut(s) 115
MluCI AATT 5 cut(s) 10, 68, 91, 147, 374
MluNI TGGCCA 1 cut(s) 115
Mox20I TGGCCA 1 cut(s) 115
MroXI GAANNNNTTC 1 cut(s) 10
MscI TGGCCA 1 cut(s) 115
MseI TTAA 5 cut(s) 59, 317, 344, 369, 499
Msp20I TGGCCA 1 cut(s) 115
MspA1I CMGCKG 2 cut(s) 215, 335
MvnI CGCG 2 cut(s) 215, 335
MwoI GCNNNNNNNGC 1 cut(s) 71
NdeII GATC 3 cut(s) 49, 121, 217
NlaIII CATG 2 cut(s) 114, 168
NlaIV GGNNCC 1 cut(s) 219
NspI RCATGY 1 cut(s) 168
PdmI GAANNNNTTC 1 cut(s) 10
PspN4I GGNNCC 1 cut(s) 219
PstI CTGCAG 1 cut(s) 433
PsuI RGATCY 1 cut(s) 217
RsaI GTAC 5 cut(s) 157, 286, 330, 351, 427
RsaNI GTAC 5 cut(s) 156, 285, 329, 350, 426
SacII CCGCGG 2 cut(s) 216, 336
SaqAI TTAA 5 cut(s) 59, 317, 344, 369, 499
Sau3AI GATC 3 cut(s) 49, 121, 217
SfcI CTRYAG 1 cut(s) 429
Sfr303I CCGCGG 2 cut(s) 216, 336
SgrBI CCGCGG 2 cut(s) 216, 336
Sse9I AATT 5 cut(s) 10, 68, 91, 147, 374
SsiI CCGC 5 cut(s) 108, 213, 215, 333, 335
SspI AATATT 1 cut(s) 243
TaaI ACNGT 3 cut(s) 289, 349, 412
TaiI ACGT 1 cut(s) 174
TasI AATT 5 cut(s) 10, 68, 91, 147, 374
Tru1I TTAA 5 cut(s) 59, 317, 344, 369, 499
Tru9I TTAA 5 cut(s) 59, 317, 344, 369, 499
TscAI CASTG 1 cut(s) 294
TspRI CASTG 1 cut(s) 294
XapI RAATTY 1 cut(s) 374
XceI RCATGY 1 cut(s) 168
XmnI GAANNNNTTC 1 cut(s) 10
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.