RchiOBHm_Chr1g0330741

Belongs to the ClpA ClpB family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
1
OG0021286
Physical Location & Seq
Forward (+)
20514330 .. 20514689
360 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ55988

Sequence Viewer

Length: 315 bp
ATGGCCTTGGCCAGCTATCTCTTCAACACAGAAAATGCTCTGGTTAGGATTGATATGAGTGAGTACATGGAACAGCATGCAGTTTCACATTTGGTGGGTGCTCCGCCTGGTTATGTTGGTTATGAAGAAGGTGGACAACTAACAGAAGTGAGAGAGCACATCAGGATGATTGAAATTTTGTTACAACTGTTGGATGATGGAAGGATTACCGACTCGCAAGGAAGGACTGTAAGCTTCACCAATTGTGTTGTGATAATGAACTCAAATCTTGGCTCCCGCTATATACTTGAAACTCTTCGTAGTACTCTAAGATAG

Protein Analysis

104

Amino Acids

11.72

Weight (kDa)

5.02

Isoelectric Point (pI)

56.08

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
AAA_2 PF07724 2 - 98 1.1e-26 AAA domain (Cdc48 subfamily)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0021286)

Species Orthologous Gene IDs
pyrus_communis pycom15g03560
rosa_chinensis RchiOBHm_Chr1g0330741 RchiOBHm_Chr7g0224361
rosa_multiflora Rmu_co8256943.1_g000001
rosa_samantha Rh7DG366900

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 2 cut(s) 104, 277
AcoI YGGCCR 1 cut(s) 9
AcsI RAATTY 1 cut(s) 174
AfaI GTAC 2 cut(s) 65, 304
AgsI TTSAA 3 cut(s) 25, 173, 290
AjnI CCWGG 1 cut(s) 106
AluBI AGCT 2 cut(s) 15, 234
AluI AGCT 2 cut(s) 15, 234
Alw21I GWGCWC 2 cut(s) 103, 159
AoxI GGCC 2 cut(s) 3, 9
ApoI RAATTY 1 cut(s) 174
Asp700I GAANNNNTTC 1 cut(s) 294
AsuHPI GGTGA 1 cut(s) 229
BalI TGGCCA 1 cut(s) 11
Bbv12I GWGCWC 2 cut(s) 103, 159
BccI CCATC 1 cut(s) 191
BciT130I CCWGG 1 cut(s) 108
BmcAI AGTACT 1 cut(s) 304
Bme1390I CCNGG 1 cut(s) 108
BmiI GGNNCC 1 cut(s) 274
BmrFI CCNGG 1 cut(s) 108
BsaJI CCNNGG 1 cut(s) 6
BseBI CCWGG 1 cut(s) 108
BseDI CCNNGG 1 cut(s) 6
BseGI GGATG 2 cut(s) 171, 199
BshFI GGCC 2 cut(s) 5, 11
BsiHKAI GWGCWC 2 cut(s) 103, 159
BsnI GGCC 2 cut(s) 5, 11
Bsp1286I GDGCHC 2 cut(s) 103, 159
BspACI CCGC 2 cut(s) 104, 277
BspANI GGCC 2 cut(s) 5, 11
BspLI GGNNCC 1 cut(s) 274
BssECI CCNNGG 1 cut(s) 6
BssT1I CCWWGG 1 cut(s) 6
Bst2UI CCWGG 1 cut(s) 108
Bst4CI ACNGT 2 cut(s) 189, 229
Bst6I CTCTTC 2 cut(s) 26, 300
BstC8I GCNNGC 2 cut(s) 13, 78
BstDEI CTNAG 1 cut(s) 308
BstF5I GGATG 2 cut(s) 171, 199
BstNI CCWGG 1 cut(s) 108
BstNSI RCATGY 1 cut(s) 80
BstSCI CCNGG 1 cut(s) 106
BsuRI GGCC 2 cut(s) 5, 11
BtsCI GGATG 2 cut(s) 171, 199
Cac8I GCNNGC 2 cut(s) 13, 78
Csp6I GTAC 2 cut(s) 64, 303
CviAII CATG 2 cut(s) 67, 77
CviJI RGCY 5 cut(s) 5, 11, 15, 234, 273
CviKI_1 RGCY 5 cut(s) 5, 11, 15, 234, 273
CviQI GTAC 2 cut(s) 64, 303
DdeI CTNAG 1 cut(s) 308
EaeI YGGCCR 1 cut(s) 9
Eam1104I CTCTTC 2 cut(s) 26, 300
EarI CTCTTC 2 cut(s) 26, 300
EciI GGCGGA 1 cut(s) 93
Eco130I CCWWGG 1 cut(s) 6
EcoRII CCWGG 1 cut(s) 106
EcoT14I CCWWGG 1 cut(s) 6
ErhI CCWWGG 1 cut(s) 6
FaeI CATG 2 cut(s) 70, 80
FaiI YATR 7 cut(s) 56, 68, 78, 114, 123, 282, 284
FatI CATG 2 cut(s) 66, 76
FauI CCCGC 1 cut(s) 284
FokI GGATG 2 cut(s) 178, 206
HaeIII GGCC 2 cut(s) 5, 11
Hin1II CATG 2 cut(s) 70, 80
HindIII AAGCTT 1 cut(s) 232
HinfI GANTC 1 cut(s) 212
HphI GGTGA 1 cut(s) 229
Hpy166II GTNNAC 1 cut(s) 134
Hpy188III TCNNGA 1 cut(s) 163
Hpy8I GTNNAC 1 cut(s) 134
HpyAV CCTTC 3 cut(s) 122, 195, 216
HpyCH4III ACNGT 2 cut(s) 189, 229
HpyCH4V TGCA 1 cut(s) 80
HpyF3I CTNAG 1 cut(s) 308
Hsp92II CATG 2 cut(s) 70, 80
LmnI GCTCC 2 cut(s) 106, 278
LpnPI CCDG 5 cut(s) 25, 26, 93, 120, 148
MaeIII GTNAC 1 cut(s) 180
MboII GAAGA 3 cut(s) 13, 137, 287
MfeI CAATTG 1 cut(s) 241
MhlI GDGCHC 2 cut(s) 103, 159
MlsI TGGCCA 1 cut(s) 11
MluCI AATT 2 cut(s) 174, 241
MluNI TGGCCA 1 cut(s) 11
MlyI GAGTC 1 cut(s) 206
MmeI TCCRAC 1 cut(s) 171
Mox20I TGGCCA 1 cut(s) 11
MroXI GAANNNNTTC 1 cut(s) 294
MscI TGGCCA 1 cut(s) 11
MslI CAYNNNNRTG 1 cut(s) 164
Msp20I TGGCCA 1 cut(s) 11
MspR9I CCNGG 1 cut(s) 108
MunI CAATTG 1 cut(s) 241
MvaI CCWGG 1 cut(s) 108
NlaIII CATG 2 cut(s) 70, 80
NlaIV GGNNCC 1 cut(s) 274
NspI RCATGY 1 cut(s) 80
PaeI GCATGC 1 cut(s) 80
PdmI GAANNNNTTC 1 cut(s) 294
PleI GAGTC 1 cut(s) 206
PpsI GAGTC 1 cut(s) 206
Psp6I CCWGG 1 cut(s) 106
PspGI CCWGG 1 cut(s) 106
PspN4I GGNNCC 1 cut(s) 274
RsaI GTAC 2 cut(s) 65, 304
RsaNI GTAC 2 cut(s) 64, 303
RseI CAYNNNNRTG 1 cut(s) 164
ScaI AGTACT 1 cut(s) 304
SchI GAGTC 1 cut(s) 206
ScrFI CCNGG 1 cut(s) 108
SduI GDGCHC 2 cut(s) 103, 159
SetI ASST 3 cut(s) 17, 133, 236
SmiMI CAYNNNNRTG 1 cut(s) 164
SphI GCATGC 1 cut(s) 80
Sse9I AATT 2 cut(s) 174, 241
SsiI CCGC 2 cut(s) 104, 277
StyD4I CCNGG 1 cut(s) 106
StyI CCWWGG 1 cut(s) 6
TaaI ACNGT 2 cut(s) 189, 229
TasI AATT 2 cut(s) 174, 241
TatI WGTACW 2 cut(s) 63, 302
TspDTI ATGAA 2 cut(s) 138, 272
XapI RAATTY 1 cut(s) 174
XceI RCATGY 1 cut(s) 80
XmnI GAANNNNTTC 1 cut(s) 294
ZrmI AGTACT 1 cut(s) 304
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.