Rh7DG366900

Belongs to the ClpA ClpB family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr7D
OG0021286
Physical Location & Seq
Reverse (-)
49333823 .. 49334536
714 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh7DG366900.1

Sequence Viewer

Length: 714 bp
ATGGAAGAGACCCTCCATAAGCGGGTTATTAGTCAGGATGAAGCAGTCAGCGCTATTAGCAGAGCTATTCGTCGAGCTCATGTTGGACTGAGGAATCCTAATCGACCAAATGCCAGTTTCATCTTTTGTGGACCAACAGGTGTTGGAAAATCTGAGCTCGTAAAAACTTTGGCGACTAGCTATTTTGGCTCTGAAGAATCAATGGTGAGGCTTGATATGAGTGAGTTCATGGAAGAGCACTCTGTTTCCAAGCTCATTGGTTCTCCCCCTGGTTATGTTGGTTACACAGAAGGCGGTCAGCTGGTTGAAGCCGTTCGTCGCCGTCCTTACACTATGGTGCTTTTTGATGAGATTAAGAAGGCTCATCCTAAAATTTTCAACTTGATGCTGCAAATACTTGAGGATGGAAGATTGACTGACAGCAAAGGCAGAACTGTAGATTTCAAGAATACACTAATCATTATGACGTCTAATGTTGGAAGCAGTGAGATTGAGAAAGGAGTGAGATTGGACCTAGCCATGGATGAAGACAAGGATTGCAGTTACAAACAAATTAAGGGCTTGGTGACAGAGAAATTGAAACACCACTTTCGACCAGAGTTCTTAAATAGGTTGGATCACTTGGATGAAATCATCAGTTTTTCGGCAGCTGACAACGTTGGACGTAAAGGAAATTGCAGATATAATGCTGAACGAGTATTATGGAACGCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0004176 GO:0005488 GO:0005515 GO:0005575 GO:0005618 GO:0005622 GO:0005623 GO:0005737 GO:0005739 GO:0006508 GO:0006605 GO:0006807 GO:0006810 GO:0006886 GO:0006996 GO:0008104 GO:0008150 GO:0008152 GO:0008233 GO:0009507 GO:0009526 GO:0009528 GO:0009532 GO:0009534 GO:0009535 GO:0009536 GO:0009570 GO:0009579 GO:0009657 GO:0009658 GO:0009706 GO:0009719 GO:0009725 GO:0009735 GO:0009889 GO:0009941 GO:0009987 GO:0010033 GO:0010380 GO:0015031 GO:0015833 GO:0016020 GO:0016043 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017038 GO:0017111 GO:0019222 GO:0019538 GO:0019866 GO:0022607 GO:0030312 GO:0031090 GO:0031323 GO:0031326 GO:0031897 GO:0031967 GO:0031969 GO:0031975 GO:0031976 GO:0031984 GO:0032991 GO:0033036 GO:0033365 GO:0034214 GO:0034357 GO:0034613 GO:0042170 GO:0042221 GO:0042623 GO:0042651 GO:0042802 GO:0042803 GO:0042886 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043933 GO:0044085 GO:0044238 GO:0044422 GO:0044424 GO:0044425 GO:0044434 GO:0044435 GO:0044436 GO:0044444 GO:0044446 GO:0044464 GO:0045036 GO:0045037 GO:0045184 GO:0046907 GO:0046983 GO:0050789 GO:0050794 GO:0050896 GO:0051171 GO:0051179 GO:0051193 GO:0051234 GO:0051259 GO:0051641 GO:0051649 GO:0055035 GO:0055085 GO:0065002 GO:0065003 GO:0065007 GO:0070011 GO:0070727 GO:0071702 GO:0071704 GO:0071705 GO:0071806 GO:0071840 GO:0071944 GO:0072594 GO:0072596 GO:0072598 GO:0090056 GO:0098796 GO:0140096 GO:1901401 GO:1901463 GO:1901564
KEGG Pathways
Metabolic & Signaling
ko01100 map01100
Pfam Domains
Protein Families
AAA AAA_2 ClpB_D2-small Clp_N UVR

Protein Analysis

237

Amino Acids

26.77

Weight (kDa)

7.75

Isoelectric Point (pI)

40.85

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
AAA_2 PF07724 35 - 207 1.3e-65 AAA domain (Cdc48 subfamily)
AAA_5 PF07728 40 - 161 1e-11 AAA domain (dynein-related subfamily)
AAA PF00004 41 - 166 1.8e-06 ATPase family associated with various cellular activities (AAA)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0021286)

Species Orthologous Gene IDs
pyrus_communis pycom15g03560
rosa_chinensis RchiOBHm_Chr1g0330741 RchiOBHm_Chr7g0224361
rosa_multiflora Rmu_co8256943.1_g000001
rosa_samantha Rh7DG366900

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 470
AciI CCGC 2 cut(s) 22, 294
AclI AACGTT 1 cut(s) 657
AclWI GGATC 1 cut(s) 624
AcsI RAATTY 1 cut(s) 372
AcuI CTGAAG 1 cut(s) 213
AcyI GRCGYC 1 cut(s) 467
AfeI AGCGCT 1 cut(s) 52
AfiI CCNNNNNNNGG 2 cut(s) 22, 520
AgsI TTSAA 4 cut(s) 308, 379, 445, 580
AjnI CCWGG 1 cut(s) 268
AleI CACNNNNGTG 1 cut(s) 335
AluBI AGCT 7 cut(s) 65, 77, 157, 180, 253, 301, 650
AluI AGCT 7 cut(s) 65, 77, 157, 180, 253, 301, 650
Alw21I GWGCWC 3 cut(s) 79, 159, 240
Alw26I GTCTC 1 cut(s) 2
AlwI GGATC 1 cut(s) 624
Aor51HI AGCGCT 1 cut(s) 52
ApeKI GCWGC 2 cut(s) 388, 647
ApoI RAATTY 1 cut(s) 372
Asp700I GAANNNNTTC 1 cut(s) 312
AspLEI GCGC 1 cut(s) 53
AspS9I GGNCC 2 cut(s) 131, 511
AsuHPI GGTGA 2 cut(s) 217, 577
AvaII GGWCC 2 cut(s) 131, 511
BanII GRGCYC 2 cut(s) 79, 159
BbsI GAAGAC 1 cut(s) 534
Bbv12I GWGCWC 3 cut(s) 79, 159, 240
BbvI GCAGC 2 cut(s) 375, 659
BccI CCATC 1 cut(s) 398
BceAI ACGGC 2 cut(s) 296, 306
BciT130I CCWGG 1 cut(s) 270
BcoDI GTCTC 1 cut(s) 2
BfaI CTAG 2 cut(s) 177, 515
BfmI CTRYAG 1 cut(s) 435
BfoI RGCGCY 1 cut(s) 54
BisI GCNGC 2 cut(s) 389, 648
BlsI GCNGC 2 cut(s) 390, 649
Bme1390I CCNGG 1 cut(s) 270
Bme18I GGWCC 2 cut(s) 131, 511
BmgT120I GGNCC 2 cut(s) 131, 511
BmrFI CCNGG 1 cut(s) 270
BmsI GCATC 1 cut(s) 375
BpiI GAAGAC 1 cut(s) 534
BpuEI CTTGAG 1 cut(s) 419
BsaHI GRCGYC 1 cut(s) 467
BsaI GGTCTC 1 cut(s) 2
BsaJI CCNNGG 2 cut(s) 268, 519
Bsc4I CCNNNNNNNGG 2 cut(s) 22, 520
Bse1I ACTGG 1 cut(s) 114
BseBI CCWGG 1 cut(s) 270
BseDI CCNNGG 2 cut(s) 268, 519
BseGI GGATG 5 cut(s) 43, 364, 409, 529, 631
BseLI CCNNNNNNNGG 2 cut(s) 22, 520
BseMII CTCAG 2 cut(s) 80, 144
BseNI ACTGG 1 cut(s) 114
BseXI GCAGC 2 cut(s) 375, 659
BsiHKAI GWGCWC 3 cut(s) 79, 159, 240
BslI CCNNNNNNNGG 2 cut(s) 22, 520
BsmAI GTCTC 1 cut(s) 2
Bso31I GGTCTC 1 cut(s) 2
Bsp1286I GDGCHC 3 cut(s) 79, 159, 240
Bsp143I GATC 1 cut(s) 616
Bsp19I CCATGG 1 cut(s) 519
BspACI CCGC 2 cut(s) 22, 294
BspCNI CTCAG 2 cut(s) 81, 145
BspPI GGATC 1 cut(s) 624
BspQI GCTCTTC 1 cut(s) 228
BspTNI GGTCTC 1 cut(s) 2
BsrI ACTGG 1 cut(s) 114
BssECI CCNNGG 2 cut(s) 268, 519
BssMI GATC 1 cut(s) 616
BssNI GRCGYC 1 cut(s) 467
BssT1I CCWWGG 1 cut(s) 519
Bst2UI CCWGG 1 cut(s) 270
Bst4CI ACNGT 1 cut(s) 436
Bst6I CTCTTC 1 cut(s) 228
BstACI GRCGYC 1 cut(s) 467
BstDEI CTNAG 2 cut(s) 89, 153
BstDSI CCRYGG 1 cut(s) 519
BstF5I GGATG 5 cut(s) 43, 364, 409, 529, 631
BstH2I RGCGCY 1 cut(s) 54
BstHHI GCGC 1 cut(s) 53
BstKTI GATC 1 cut(s) 619
BstMAI GTCTC 1 cut(s) 2
BstMBI GATC 1 cut(s) 616
BstMWI GCNNNNNNNGC 3 cut(s) 50, 57, 186
BstNI CCWGG 1 cut(s) 270
BstSCI CCNGG 1 cut(s) 268
BstSFI CTRYAG 1 cut(s) 435
BstV1I GCAGC 2 cut(s) 375, 659
BstV2I GAAGAC 1 cut(s) 534
BtgI CCRYGG 1 cut(s) 519
BtsCI GGATG 5 cut(s) 43, 364, 409, 529, 631
BtsI GCAGTG 1 cut(s) 490
BtsIMutI CAGTG 1 cut(s) 490
CfoI GCGC 1 cut(s) 53
Cfr13I GGNCC 2 cut(s) 131, 511
CviAII CATG 3 cut(s) 80, 229, 520
DdeI CTNAG 2 cut(s) 89, 153
DpnI GATC 1 cut(s) 618
DpnII GATC 1 cut(s) 616
Eam1104I CTCTTC 1 cut(s) 228
EarI CTCTTC 1 cut(s) 228
Ecl136II GAGCTC 2 cut(s) 77, 157
Eco130I CCWWGG 1 cut(s) 519
Eco24I GRGCYC 2 cut(s) 79, 159
Eco31I GGTCTC 1 cut(s) 2
Eco47I GGWCC 2 cut(s) 131, 511
Eco47III AGCGCT 1 cut(s) 52
Eco53kI GAGCTC 2 cut(s) 77, 157
Eco57I CTGAAG 1 cut(s) 213
EcoICRI GAGCTC 2 cut(s) 77, 157
EcoRII CCWGG 1 cut(s) 268
EcoT14I CCWWGG 1 cut(s) 519
EcoT38I GRGCYC 2 cut(s) 79, 159
ErhI CCWWGG 1 cut(s) 519
FaeI CATG 3 cut(s) 83, 232, 523
FatI CATG 3 cut(s) 79, 228, 519
FauI CCCGC 1 cut(s) 15
Fnu4HI GCNGC 2 cut(s) 389, 648
FokI GGATG 5 cut(s) 50, 351, 416, 536, 638
FriOI GRGCYC 2 cut(s) 79, 159
Fsp4HI GCNGC 2 cut(s) 389, 648
FspBI CTAG 2 cut(s) 177, 515
GlaI GCGC 1 cut(s) 52
GluI GCNGC 2 cut(s) 389, 648
HaeII RGCGCY 1 cut(s) 54
HhaI GCGC 1 cut(s) 53
Hin1I GRCGYC 1 cut(s) 467
Hin1II CATG 3 cut(s) 83, 232, 523
Hin6I GCGC 1 cut(s) 51
HinP1I GCGC 1 cut(s) 51
HinfI GANTC 2 cut(s) 94, 197
HphI GGTGA 2 cut(s) 217, 577
Hpy166II GTNNAC 1 cut(s) 131
Hpy188I TCNGA 2 cut(s) 154, 193
Hpy188III TCNNGA 2 cut(s) 35, 445
Hpy8I GTNNAC 1 cut(s) 131
Hpy99I CGWCG 2 cut(s) 75, 321
HpyAV CCTTC 2 cut(s) 284, 352
HpyCH4III ACNGT 1 cut(s) 436
HpyCH4IV ACGT 3 cut(s) 467, 657, 664
HpyCH4V TGCA 3 cut(s) 391, 540, 678
HpyF10VI GCNNNNNNNGC 3 cut(s) 50, 57, 186
HpyF3I CTNAG 2 cut(s) 89, 153
HpySE526I ACGT 3 cut(s) 467, 657, 664
Hsp92I GRCGYC 1 cut(s) 467
Hsp92II CATG 3 cut(s) 83, 232, 523
HspAI GCGC 1 cut(s) 51
Kzo9I GATC 1 cut(s) 616
LguI GCTCTTC 1 cut(s) 228
LpnPI CCDG 7 cut(s) 20, 123, 127, 255, 282, 287, 609
Lsp1109I GCAGC 2 cut(s) 375, 659
LweI GCATC 1 cut(s) 375
MaeI CTAG 2 cut(s) 177, 515
MaeII ACGT 3 cut(s) 467, 657, 664
MaeIII GTNAC 3 cut(s) 281, 542, 565
MalI GATC 1 cut(s) 618
MboI GATC 1 cut(s) 616
MboII GAAGA 5 cut(s) 17, 206, 245, 420, 539
MhlI GDGCHC 3 cut(s) 79, 159, 240
MluCI AATT 4 cut(s) 372, 552, 575, 673
MmeI TCCRAC 5 cut(s) 64, 124, 457, 594, 640
MnlI CCTC 4 cut(s) 23, 84, 201, 394
MroXI GAANNNNTTC 1 cut(s) 312
MseI TTAA 4 cut(s) 354, 555, 605, 712
MslI CAYNNNNRTG 2 cut(s) 335, 624
MspA1I CMGCKG 2 cut(s) 301, 650
MspR9I CCNGG 1 cut(s) 270
MvaI CCWGG 1 cut(s) 270
MwoI GCNNNNNNNGC 3 cut(s) 50, 57, 186
NcoI CCATGG 1 cut(s) 519
NdeII GATC 1 cut(s) 616
NlaIII CATG 3 cut(s) 83, 232, 523
NmuCI GTSAC 1 cut(s) 565
OliI CACNNNNGTG 1 cut(s) 335
PciSI GCTCTTC 1 cut(s) 228
PdmI GAANNNNTTC 1 cut(s) 312
PfeI GAWTC 2 cut(s) 94, 197
PkrI GCNGC 2 cut(s) 390, 649
Psp124BI GAGCTC 2 cut(s) 79, 159
Psp1406I AACGTT 1 cut(s) 657
Psp6I CCWGG 1 cut(s) 268
PspGI CCWGG 1 cut(s) 268
PspPI GGNCC 2 cut(s) 131, 511
PvuII CAGCTG 2 cut(s) 301, 650
RseI CAYNNNNRTG 2 cut(s) 335, 624
SacI GAGCTC 2 cut(s) 79, 159
SapI GCTCTTC 1 cut(s) 228
SaqAI TTAA 4 cut(s) 354, 555, 605, 712
SatI GCNGC 2 cut(s) 389, 648
Sau3AI GATC 1 cut(s) 616
Sau96I GGNCC 2 cut(s) 131, 511
ScrFI CCNGG 1 cut(s) 270
SduI GDGCHC 3 cut(s) 79, 159, 240
SfaNI GCATC 1 cut(s) 375
SfcI CTRYAG 1 cut(s) 435
SinI GGWCC 2 cut(s) 131, 511
SmiMI CAYNNNNRTG 2 cut(s) 335, 624
SmlI CTYRAG 1 cut(s) 398
SmoI CTYRAG 1 cut(s) 398
Sse9I AATT 4 cut(s) 372, 552, 575, 673
SsiI CCGC 2 cut(s) 22, 294
SspMI CTAG 2 cut(s) 177, 515
SstI GAGCTC 2 cut(s) 79, 159
StyD4I CCNGG 1 cut(s) 268
StyI CCWWGG 1 cut(s) 519
TaaI ACNGT 1 cut(s) 436
TaiI ACGT 3 cut(s) 470, 660, 667
TaqI TCGA 3 cut(s) 73, 103, 592
TasI AATT 4 cut(s) 372, 552, 575, 673
TfiI GAWTC 2 cut(s) 94, 197
Tru1I TTAA 4 cut(s) 354, 555, 605, 712
Tru9I TTAA 4 cut(s) 354, 555, 605, 712
TscAI CASTG 1 cut(s) 490
TseFI GTSAC 1 cut(s) 565
TseI GCWGC 2 cut(s) 388, 647
Tsp45I GTSAC 1 cut(s) 565
TspDTI ATGAA 5 cut(s) 54, 109, 217, 540, 642
TspRI CASTG 1 cut(s) 490
VpaK11BI GGWCC 2 cut(s) 131, 511
XapI RAATTY 1 cut(s) 372
XmnI GAANNNNTTC 1 cut(s) 312
XspI CTAG 2 cut(s) 177, 515
ZraI GACGTC 1 cut(s) 468
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.