RchiOBHm_Chr1g0356211

LRR receptor-like serine threonine-protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
1
OG0018146
Physical Location & Seq
Forward (+)
48757609 .. 48761486
3878 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ58160

Sequence Viewer

Length: 1299 bp
ATGGGACGATCCGGACATCTTCCAGAAAATTTGATTGGTGTGGGTACTTTTGGGTCTGTATATAAAGTATTTATTGATAGAGCTCAAGTACTTGTTGCTGTGAAGGTATTTAACTTATTACACCAAGGGTCTTTGAAGAGTTTCATAGCCGAATGTGAGGTGTTGCGAAGTATCAGGCATAGCAACATCCTGCACCCAACTGTTGGAACACAAAAGGTAAGAAGCGCACCCAAGAATTTAAGTTTTGTTCAGAGGCTAGACATTGCCATTGATGTTGCTTGTGCACTGGATTATCTTCATAATCATTGTGATACACCAACAGTTCATTGTGATCTAAAGCCGAGCAATATTCTTTTGGACAATGACTTGACTGGACGGGTTTCTGACTTTGGACTAGCAAGATTTCTCTCCAAACCCAATAACATTTCTACAAATCTACCAAGTGTCATTAAATTAAGAGGACCTGTTAGCTATGTTGCGCCAGAGTATGAAACGGGAAGTGAGGCATCAACATATGGGGATGTGTATAGCTTTGGGATTCTCTTGTTAGAGATGTTTACAGGAAAGAAATCCACTGACGATATGTTCAGTTACCGCTTGAACCTTCATAAATTTGTGAGTATGTCTTACTTTGAGGGAGTATTTTCAGAGATTGCAGATCCAAGACTGCTTCTTCAAGGAGGCAACAGTCGTCCCAGTAAATGCAGTGTGAAATTCAAATCGGAAATTGGGCAGTGCTTGAGATCGATATTTGAAATTGGAATTGTGTGTTCCAGTGAGTCCCCGAGAGGCAGAATGGATATACATAATGTTGTATTTGAATTGAATTCCGTTAGGGATGACTTTCTGAATAGGCTACATAGTTATAGTCCTACTGTTGGTGTAAGGTCAAGATCATGCAGCAGCTGCATCGTTTGTCGACGCAGGATGAAGGAACCACCTGCATGGAGGTCATGGTCAAACCGTGGCTACCGCCAGTATGGTACGGGAAGTGGAGAGCGAAAATACTGGGATGTTTACAGCTTTGGCATTCTCTTGTTAGAAATGTTTACCGGAAAAGGACCCACAGATCTCATGAATATTCATAATTTTGTAGAGATGGCTATTCCTGAACGAGTTACAGAAATTGCAGATTCAAGACTGCTTCAAAGAGGCACCAATGATACTCAAAAAAATGAGTGCTTGATTTCGATATTTGAAATTGGAATTGCATGTTCAGATGGAGCCCAAAGTGACCGAATGAGTATCGATCGTGTTGTGCCTAGATTGCATTCCATTAGAGACCGTCTTCTCCCATAG

Protein Analysis

432

Amino Acids

48.49

Weight (kDa)

8.76

Isoelectric Point (pI)

45.53

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pkinase PF00069 10 - 65 8e-07 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 73 - 196 7.8e-21 Protein tyrosine and serine/threonine kinase
Pkinase PF00069 87 - 204 1.4e-20 Protein kinase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0018146)

Species Orthologous Gene IDs
rosa_chinensis RchiOBHm_Chr1g0356211 RchiOBHm_Chr1g0356491
rosa_laevigata RLG00000028092 RLG00000028093 RLG00000028099
rosa_multiflora Rmu_sc0000281.1_g000018 Rmu_sc0014206.1_g000003
rosa_roxburghii Rroxscaffold_4G00299490
rosa_rugosa Rorug01G0250100 Rorug01G0250100
rosa_wichuraiana Rw1G023310

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 949
Acc36I ACCTGC 1 cut(s) 949
AccB1I GGYRCC 1 cut(s) 1154
AccB7I CCANNNNNTGG 1 cut(s) 203
AccI GTMKAC 1 cut(s) 919
AccIII TCCGGA 1 cut(s) 11
AciI CCGC 2 cut(s) 595, 973
AclWI GGATC 2 cut(s) 3, 653
AcsI RAATTY 5 cut(s) 28, 235, 611, 713, 826
AfaI GTAC 3 cut(s) 46, 90, 985
AfiI CCNNNNNNNGG 2 cut(s) 203, 878
AjuI GAANNNNNNNTTGG 2 cut(s) 18, 50
AluBI AGCT 5 cut(s) 83, 471, 531, 906, 1023
AluI AGCT 5 cut(s) 83, 471, 531, 906, 1023
Alw21I GWGCWC 2 cut(s) 85, 286
Alw26I GTCTC 1 cut(s) 1275
Alw44I GTGCAC 1 cut(s) 282
AlwI GGATC 2 cut(s) 3, 653
AlwNI CAGNNNCTG 1 cut(s) 906
Ama87I CYCGRG 1 cut(s) 784
Aor13HI TCCGGA 1 cut(s) 11
ApaLI GTGCAC 1 cut(s) 282
ApeKI GCWGC 3 cut(s) 900, 903, 906
ApoI RAATTY 5 cut(s) 28, 235, 611, 713, 826
Asp700I GAANNNNTTC 1 cut(s) 140
AspLEI GCGC 2 cut(s) 227, 481
AspS9I GGNCC 2 cut(s) 461, 1061
AvaI CYCGRG 1 cut(s) 784
AvaII GGWCC 2 cut(s) 461, 1061
BaeGI GKGCMC 1 cut(s) 286
BanI GGYRCC 1 cut(s) 1154
BanII GRGCYC 2 cut(s) 85, 1228
BbsI GAAGAC 1 cut(s) 1280
Bbv12I GWGCWC 2 cut(s) 85, 286
BbvI GCAGC 3 cut(s) 893, 912, 915
BccI CCATC 2 cut(s) 1093, 1214
BcgI CGANNNNNNTGC 2 cut(s) 892, 926
BcoDI GTCTC 1 cut(s) 1275
BfaI CTAG 3 cut(s) 257, 395, 1263
BfuAI ACCTGC 1 cut(s) 949
BglII AGATCT 1 cut(s) 1069
BisI GCNGC 3 cut(s) 901, 904, 907
BlsI GCNGC 3 cut(s) 902, 905, 908
BmcAI AGTACT 1 cut(s) 90
Bme18I GGWCC 2 cut(s) 461, 1061
BmeT110I CYCGRG 1 cut(s) 784
BmgT120I GGNCC 2 cut(s) 461, 1061
BmiI GGNNCC 4 cut(s) 936, 1063, 1156, 1225
BmrI ACTGGG 2 cut(s) 690, 1018
BmsI GCATC 2 cut(s) 515, 918
BmuI ACTGGG 2 cut(s) 690, 1018
BpiI GAAGAC 1 cut(s) 1280
BpuEI CTTGAG 2 cut(s) 69, 760
Bsa29I ATCGAT 2 cut(s) 746, 1248
BsaI GGTCTC 1 cut(s) 1275
BsaJI CCNNGG 2 cut(s) 124, 964
BsaWI WCCGGW 2 cut(s) 11, 1052
Bsc4I CCNNNNNNNGG 2 cut(s) 203, 878
Bse1I ACTGG 6 cut(s) 291, 376, 696, 774, 976, 1013
Bse3DI GCAATG 1 cut(s) 261
BseAI TCCGGA 1 cut(s) 11
BseCI ATCGAT 2 cut(s) 746, 1248
BseDI CCNNGG 2 cut(s) 124, 964
BseGI GGATG 5 cut(s) 186, 526, 844, 933, 1018
BseLI CCNNNNNNNGG 2 cut(s) 203, 878
BseMI GCAATG 1 cut(s) 261
BseNI ACTGG 6 cut(s) 291, 376, 696, 774, 976, 1013
BseSI GKGCMC 1 cut(s) 286
BseXI GCAGC 3 cut(s) 893, 912, 915
BsgI GTGCAG 1 cut(s) 176
Bsh1285I CGRYCG 1 cut(s) 1252
BshNI GGYRCC 1 cut(s) 1154
BshVI ATCGAT 2 cut(s) 746, 1248
BsiEI CGRYCG 1 cut(s) 1252
BsiHKAI GWGCWC 2 cut(s) 85, 286
BsiHKCI CYCGRG 1 cut(s) 784
BsiSI CCGG 2 cut(s) 12, 1053
BslFI GGGAC 3 cut(s) 18, 678, 766
BslI CCNNNNNNNGG 2 cut(s) 203, 878
BsmAI GTCTC 1 cut(s) 1275
BsmFI GGGAC 3 cut(s) 18, 678, 766
BsmI GAATGC 2 cut(s) 1029, 1270
Bso31I GGTCTC 1 cut(s) 1275
BsoBI CYCGRG 1 cut(s) 784
Bsp1286I GDGCHC 3 cut(s) 85, 286, 1228
Bsp13I TCCGGA 1 cut(s) 11
Bsp143I GATC 7 cut(s) 8, 331, 658, 743, 893, 1069, 1249
BspACI CCGC 2 cut(s) 595, 973
BspDI ATCGAT 2 cut(s) 746, 1248
BspEI TCCGGA 1 cut(s) 11
BspHI TCATGA 1 cut(s) 1074
BspLI GGNNCC 4 cut(s) 936, 1063, 1156, 1225
BspMI ACCTGC 1 cut(s) 949
BspPI GGATC 2 cut(s) 3, 653
BspT107I GGYRCC 1 cut(s) 1154
BspTNI GGTCTC 1 cut(s) 1275
BsrDI GCAATG 1 cut(s) 261
BsrI ACTGG 6 cut(s) 291, 376, 696, 774, 976, 1013
BssECI CCNNGG 2 cut(s) 124, 964
BssMI GATC 7 cut(s) 8, 331, 658, 743, 893, 1069, 1249
BssT1I CCWWGG 1 cut(s) 124
Bst4CI ACNGT 6 cut(s) 202, 322, 689, 877, 965, 1286
Bst6I CTCTTC 1 cut(s) 131
BstAPI GCANNNNNTGC 1 cut(s) 906
BstDSI CCRYGG 1 cut(s) 964
BstF5I GGATG 5 cut(s) 186, 526, 844, 933, 1018
BstHHI GCGC 2 cut(s) 227, 481
BstKTI GATC 7 cut(s) 11, 334, 661, 746, 896, 1072, 1252
BstMAI GTCTC 1 cut(s) 1275
BstMBI GATC 7 cut(s) 8, 331, 658, 743, 893, 1069, 1249
BstMCI CGRYCG 1 cut(s) 1252
BstMWI GCNNNNNNNGC 2 cut(s) 906, 1267
BstNSI RCATGY 1 cut(s) 1215
BstSLI GKGCMC 1 cut(s) 286
BstV1I GCAGC 3 cut(s) 893, 912, 915
BstV2I GAAGAC 1 cut(s) 1280
BstX2I RGATCY 2 cut(s) 658, 1069
BstXI CCANNNNNNTGG 1 cut(s) 945
BstYI RGATCY 2 cut(s) 658, 1069
Bsu15I ATCGAT 2 cut(s) 746, 1248
BsuTUI ATCGAT 2 cut(s) 746, 1248
BtgI CCRYGG 1 cut(s) 964
BtsCI GGATG 5 cut(s) 186, 526, 844, 933, 1018
BtsI GCAGTG 2 cut(s) 712, 740
BtsIMutI CAGTG 5 cut(s) 284, 573, 712, 740, 781
BveI ACCTGC 1 cut(s) 949
CaiI CAGNNNCTG 1 cut(s) 906
CciI TCATGA 1 cut(s) 1074
CfoI GCGC 2 cut(s) 227, 481
Cfr13I GGNCC 2 cut(s) 461, 1061
ClaI ATCGAT 2 cut(s) 746, 1248
CseI GACGC 1 cut(s) 930
Csp6I GTAC 3 cut(s) 45, 89, 984
CviAII CATG 5 cut(s) 897, 945, 954, 1075, 1212
CviQI GTAC 3 cut(s) 45, 89, 984
DpnI GATC 7 cut(s) 10, 333, 660, 745, 895, 1071, 1251
DpnII GATC 7 cut(s) 8, 331, 658, 743, 893, 1069, 1249
Eam1104I CTCTTC 1 cut(s) 131
EarI CTCTTC 1 cut(s) 131
Ecl136II GAGCTC 1 cut(s) 83
Eco130I CCWWGG 1 cut(s) 124
Eco24I GRGCYC 2 cut(s) 85, 1228
Eco31I GGTCTC 1 cut(s) 1275
Eco47I GGWCC 2 cut(s) 461, 1061
Eco53kI GAGCTC 1 cut(s) 83
Eco88I CYCGRG 1 cut(s) 784
EcoICRI GAGCTC 1 cut(s) 83
EcoO109I RGGNCCY 2 cut(s) 461, 1061
EcoRI GAATTC 1 cut(s) 826
EcoT14I CCWWGG 1 cut(s) 124
EcoT38I GRGCYC 2 cut(s) 85, 1228
ErhI CCWWGG 1 cut(s) 124
FaeI CATG 5 cut(s) 900, 948, 957, 1078, 1215
FaqI GGGAC 3 cut(s) 18, 678, 766
FatI CATG 5 cut(s) 896, 944, 953, 1074, 1211
FauNDI CATATG 1 cut(s) 514
FblI GTMKAC 1 cut(s) 919
Fnu4HI GCNGC 3 cut(s) 901, 904, 907
FokI GGATG 5 cut(s) 173, 533, 851, 940, 1025
FriOI GRGCYC 2 cut(s) 85, 1228
Fsp4HI GCNGC 3 cut(s) 901, 904, 907
FspBI CTAG 3 cut(s) 257, 395, 1263
GlaI GCGC 2 cut(s) 226, 480
GluI GCNGC 3 cut(s) 901, 904, 907
HapII CCGG 2 cut(s) 12, 1053
HgaI GACGC 1 cut(s) 930
HhaI GCGC 2 cut(s) 227, 481
Hin1II CATG 5 cut(s) 900, 948, 957, 1078, 1215
Hin6I GCGC 2 cut(s) 225, 479
HinP1I GCGC 2 cut(s) 225, 479
HincII GTYRAC 1 cut(s) 920
HindII GTYRAC 1 cut(s) 920
HinfI GANTC 3 cut(s) 538, 779, 1133
HpaII CCGG 2 cut(s) 12, 1053
Hpy166II GTNNAC 5 cut(s) 284, 558, 920, 1018, 1050
Hpy188I TCNGA 6 cut(s) 252, 385, 649, 724, 849, 1219
Hpy188III TCNNGA 6 cut(s) 12, 23, 891, 1075, 1109, 1137
Hpy8I GTNNAC 5 cut(s) 284, 558, 920, 1018, 1050
Hpy99I CGWCG 1 cut(s) 924
HpyAV CCTTC 3 cut(s) 97, 614, 925
HpyCH4III ACNGT 6 cut(s) 202, 322, 689, 877, 965, 1286
HpyF10VI GCNNNNNNNGC 2 cut(s) 906, 1267
Hsp92II CATG 5 cut(s) 900, 948, 957, 1078, 1215
HspAI GCGC 2 cut(s) 225, 479
Kpn2I TCCGGA 1 cut(s) 11
Kzo9I GATC 7 cut(s) 8, 331, 658, 743, 893, 1069, 1249
LmnI GCTCC 1 cut(s) 1223
Lsp1109I GCAGC 3 cut(s) 893, 912, 915
LweI GCATC 2 cut(s) 515, 918
MaeI CTAG 3 cut(s) 257, 395, 1263
MaeIII GTNAC 3 cut(s) 590, 1117, 1232
MalI GATC 7 cut(s) 10, 333, 660, 745, 895, 1071, 1251
MboI GATC 7 cut(s) 8, 331, 658, 743, 893, 1069, 1249
MboII GAAGA 5 cut(s) 11, 148, 287, 665, 1280
MflI RGATCY 2 cut(s) 658, 1069
MhlI GDGCHC 3 cut(s) 85, 286, 1228
MlyI GAGTC 1 cut(s) 788
MmeI TCCRAC 1 cut(s) 184
MnlI CCTC 9 cut(s) 151, 246, 452, 496, 628, 674, 782, 942, 1145
MroI TCCGGA 1 cut(s) 11
MroXI GAANNNNTTC 1 cut(s) 140
MseI TTAA 4 cut(s) 111, 239, 450, 455
MslI CAYNNNNRTG 1 cut(s) 943
MspA1I CMGCKG 1 cut(s) 906
MspI CCGG 2 cut(s) 12, 1053
Mva1269I GAATGC 2 cut(s) 1029, 1270
MwoI GCNNNNNNNGC 2 cut(s) 906, 1267
NdeI CATATG 1 cut(s) 514
NdeII GATC 7 cut(s) 8, 331, 658, 743, 893, 1069, 1249
NlaIII CATG 5 cut(s) 900, 948, 957, 1078, 1215
NlaIV GGNNCC 4 cut(s) 936, 1063, 1156, 1225
NmeAIII GCCGAG 1 cut(s) 366
NmuCI GTSAC 1 cut(s) 1232
NspI RCATGY 1 cut(s) 1215
PagI TCATGA 1 cut(s) 1074
PaqCI CACCTGC 1 cut(s) 949
PctI GAATGC 2 cut(s) 1029, 1270
PdmI GAANNNNTTC 1 cut(s) 140
PfeI GAWTC 2 cut(s) 538, 1133
PflMI CCANNNNNTGG 1 cut(s) 203
PkrI GCNGC 3 cut(s) 902, 905, 908
Ple19I CGATCG 1 cut(s) 1252
PleI GAGTC 1 cut(s) 787
PpsI GAGTC 1 cut(s) 787
PpuMI RGGWCCY 2 cut(s) 461, 1061
Psp124BI GAGCTC 1 cut(s) 85
Psp5II RGGWCCY 2 cut(s) 461, 1061
PspN4I GGNNCC 4 cut(s) 936, 1063, 1156, 1225
PspPI GGNCC 2 cut(s) 461, 1061
PspPPI RGGWCCY 2 cut(s) 461, 1061
PstNI CAGNNNCTG 1 cut(s) 906
PsuI RGATCY 2 cut(s) 658, 1069
PvuI CGATCG 1 cut(s) 1252
PvuII CAGCTG 1 cut(s) 906
RsaI GTAC 3 cut(s) 46, 90, 985
RsaNI GTAC 3 cut(s) 45, 89, 984
RseI CAYNNNNRTG 1 cut(s) 943
SacI GAGCTC 1 cut(s) 85
SalI GTCGAC 1 cut(s) 918
SaqAI TTAA 4 cut(s) 111, 239, 450, 455
SatI GCNGC 3 cut(s) 901, 904, 907
Sau3AI GATC 7 cut(s) 8, 331, 658, 743, 893, 1069, 1249
Sau96I GGNCC 2 cut(s) 461, 1061
ScaI AGTACT 1 cut(s) 90
SchI GAGTC 1 cut(s) 788
SduI GDGCHC 3 cut(s) 85, 286, 1228
SfaNI GCATC 2 cut(s) 515, 918
SinI GGWCC 2 cut(s) 461, 1061
SmiMI CAYNNNNRTG 1 cut(s) 943
SmlI CTYRAG 2 cut(s) 84, 739
SmoI CTYRAG 2 cut(s) 84, 739
SsiI CCGC 2 cut(s) 595, 973
SspI AATATT 2 cut(s) 349, 1081
SspMI CTAG 3 cut(s) 257, 395, 1263
SstI GAGCTC 1 cut(s) 85
StyI CCWWGG 1 cut(s) 124
TaaI ACNGT 6 cut(s) 202, 322, 689, 877, 965, 1286
TaqI TCGA 4 cut(s) 746, 919, 1190, 1248
TaqII GACCGA 1 cut(s) 1251
TatI WGTACW 1 cut(s) 88
TfiI GAWTC 2 cut(s) 538, 1133
Tru1I TTAA 4 cut(s) 111, 239, 450, 455
Tru9I TTAA 4 cut(s) 111, 239, 450, 455
TscAI CASTG 5 cut(s) 291, 580, 712, 740, 781
TseFI GTSAC 1 cut(s) 1232
TseI GCWGC 3 cut(s) 900, 903, 906
Tsp45I GTSAC 1 cut(s) 1232
TspDTI ATGAA 8 cut(s) 133, 287, 314, 504, 596, 944, 1073, 1091
TspGWI ACGGA 1 cut(s) 820
TspRI CASTG 5 cut(s) 291, 580, 712, 740, 781
Van91I CCANNNNNTGG 1 cut(s) 203
VneI GTGCAC 1 cut(s) 282
VpaK11BI GGWCC 2 cut(s) 461, 1061
XapI RAATTY 5 cut(s) 28, 235, 611, 713, 826
XceI RCATGY 1 cut(s) 1215
XmiI GTMKAC 1 cut(s) 919
XmnI GAANNNNTTC 1 cut(s) 140
XspI CTAG 3 cut(s) 257, 395, 1263
ZrmI AGTACT 1 cut(s) 90
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.