RLG00000028093
ERF Family

Belongs to the protein kinase superfamily. Ser Thr protein kinase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr6
OG0018146
Physical Location & Seq
Reverse (-)
18613121 .. 18616467
3347 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000028093

Sequence Viewer

Length: 1065 bp
ATGGAGAATGGTAGCTTGGAAGAGTGGCTGCATCCAATTATTGGAAACAAAACTTTGGACAATGACTTGACTGGCTGGGTTTCTGACTTTGGACTGGCAAAGTTTCTCTCAGAAACCAGTAATGTTGCTTCAAATCAATCAAGCTCTATTGGAGTTCGAAGACCAGTTGGTTATGCTGCACCAGGAAACATCGACAGAGGAACCAAAAGGGACAATTCGAACTTCTTCCAGGTTTCATATAATACACTTCTAAAAGCTACTGATAGGTTTTCTTCAGAAAATTTGATTGGTGTGGGTACTTTTGGGTCTGTGTATAAAGTATTTCTTGATAGAGCTCAAGTACTTGTTGCTGTGAAGGTATTTAACTTATTACACCAAGGGTCTTTGAAGAGTTTCATAGCCAAATGTGAGGTGTTGCGAAGTATCAGGCATCGCAACATCGTTAAGATTATAACCTCATGTTCGAGTATTGATTTTCGTGGTAATGATTTCAAGGCTCTGGTTTATGAGTACATGGAGAATGGTACCTTGCAAGAGTGGCTGCATCCAACTGGTGGAACACAAAAGCAATATTCTTTATTTCTTTTGGACAATGACTTGACCGGACGGGCTTCTGACTTTGGACTAGCACGATTTCTTTCCAAACCCAATAACGTTTCTACAAATCTACCAAGTGTCGTTAGATTAAGAGGACCTGTTAGGTATGTTGCGCCAGAGTATGAAACGGGAAGTGAGCCATCAACATATGGGGACGTGTATAGCTTTGGGATTCTCTTGTTAGAGATGTTTACAGGAAAGAAACCCACTGACGACATGTTCAGTGACCGCTTGAACCTTCATAAATTTGTGAGTATGTCTTACTTCGAGGGAGTATTTCCAGAGATTGCAGATCCAAGACTGCTTCTTCAAGGAGGCAACAGTCCTCCCAGTAAATGCAGTGTGAAATTCAAATCGGAAATTGGGCAGTGCTTGAGATCGATATTTGAAATTGGAATTGTGTGTTCCAGTGAGTCCCCGAGAGACAAAATGGATATACATTATGTTGTTGTAAACACGGAAATCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRRNT_2 LRR_1 LRR_8 Pkinase Pkinase_Tyr

Protein Analysis

355

Amino Acids

39.48

Weight (kDa)

7.01

Isoelectric Point (pI)

37.71

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pkinase PF00069 91 - 188 1.5e-12 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 93 - 184 2.1e-11 Protein tyrosine and serine/threonine kinase
PK_Tyr_Ser-Thr PF07714 195 - 274 1.7e-10 Protein tyrosine and serine/threonine kinase
Pkinase PF00069 195 - 275 6e-09 Protein kinase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0018146)

Species Orthologous Gene IDs
rosa_chinensis RchiOBHm_Chr1g0356211 RchiOBHm_Chr1g0356491
rosa_laevigata RLG00000028092 RLG00000028093 RLG00000028099
rosa_multiflora Rmu_sc0000281.1_g000018 Rmu_sc0014206.1_g000003
rosa_roxburghii Rroxscaffold_4G00299490
rosa_rugosa Rorug01G0250100 Rorug01G0250100
rosa_wichuraiana Rw1G023310

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 452
Acc65I GGTACC 1 cut(s) 524
AccB1I GGYRCC 1 cut(s) 524
AccB7I CCANNNNNTGG 2 cut(s) 41, 554
AciI CCGC 1 cut(s) 826
AclI AACGTT 1 cut(s) 654
AclWI GGATC 1 cut(s) 884
AcsI RAATTY 3 cut(s) 280, 842, 944
AcuI CTGAAG 1 cut(s) 258
AfaI GTAC 4 cut(s) 298, 342, 512, 526
AfiI CCNNNNNNNGG 2 cut(s) 41, 554
AflIII ACRYGT 2 cut(s) 753, 813
AgsI TTSAA 7 cut(s) 132, 388, 493, 832, 908, 949, 986
AjiI CACGTC 1 cut(s) 754
AjnI CCWGG 2 cut(s) 181, 228
AjuI GAANNNNNNNTTGG 3 cut(s) 31, 270, 302
AluBI AGCT 5 cut(s) 15, 144, 257, 335, 762
AluI AGCT 5 cut(s) 15, 144, 257, 335, 762
Alw21I GWGCWC 1 cut(s) 337
Alw26I GTCTC 1 cut(s) 1014
AlwI GGATC 1 cut(s) 884
Ama87I CYCGRG 1 cut(s) 1015
ApeKI GCWGC 3 cut(s) 28, 176, 541
ApoI RAATTY 3 cut(s) 280, 842, 944
Asp700I GAANNNNTTC 2 cut(s) 224, 392
Asp718I GGTACC 1 cut(s) 524
AspLEI GCGC 1 cut(s) 712
AspS9I GGNCC 1 cut(s) 692
AsuII TTCGAA 2 cut(s) 157, 218
AvaI CYCGRG 1 cut(s) 1015
AvaII GGWCC 1 cut(s) 692
BanI GGYRCC 1 cut(s) 524
BanII GRGCYC 1 cut(s) 337
BarI GAAGNNNNNNTAC 2 cut(s) 112, 144
BbsI GAAGAC 1 cut(s) 166
Bbv12I GWGCWC 1 cut(s) 337
BbvI GCAGC 3 cut(s) 15, 163, 528
BccI CCATC 1 cut(s) 745
BciT130I CCWGG 2 cut(s) 183, 230
BcoDI GTCTC 1 cut(s) 1014
BfaI CTAG 1 cut(s) 626
BisI GCNGC 3 cut(s) 29, 177, 542
BlsI GCNGC 3 cut(s) 30, 178, 543
BmcAI AGTACT 1 cut(s) 342
Bme1390I CCNGG 2 cut(s) 183, 230
Bme18I GGWCC 1 cut(s) 692
BmeT110I CYCGRG 1 cut(s) 1015
BmgBI CACGTC 1 cut(s) 754
BmgT120I GGNCC 1 cut(s) 692
BmiI GGNNCC 2 cut(s) 202, 526
BmrFI CCNGG 2 cut(s) 183, 230
BmrI ACTGGG 1 cut(s) 921
BmsI GCATC 3 cut(s) 40, 439, 553
BmuI ACTGGG 1 cut(s) 921
BpiI GAAGAC 1 cut(s) 166
Bpu14I TTCGAA 2 cut(s) 157, 218
BpuEI CTTGAG 2 cut(s) 321, 991
Bsa29I ATCGAT 1 cut(s) 977
BsaJI CCNNGG 1 cut(s) 376
BsaWI WCCGGW 1 cut(s) 602
Bsc4I CCNNNNNNNGG 2 cut(s) 41, 554
Bse1I ACTGG 7 cut(s) 76, 99, 117, 164, 556, 927, 1005
BseBI CCWGG 2 cut(s) 183, 230
BseCI ATCGAT 1 cut(s) 977
BseDI CCNNGG 1 cut(s) 376
BseGI GGATG 2 cut(s) 31, 544
BseLI CCNNNNNNNGG 2 cut(s) 41, 554
BseMII CTCAG 1 cut(s) 123
BseNI ACTGG 7 cut(s) 76, 99, 117, 164, 556, 927, 1005
BseXI GCAGC 3 cut(s) 15, 163, 528
BseYI CCCAGC 1 cut(s) 75
BsgI GTGCAG 1 cut(s) 162
BshNI GGYRCC 1 cut(s) 524
BshVI ATCGAT 1 cut(s) 977
BsiHKAI GWGCWC 1 cut(s) 337
BsiHKCI CYCGRG 1 cut(s) 1015
BsiSI CCGG 1 cut(s) 603
BslFI GGGAC 3 cut(s) 224, 764, 997
BslI CCNNNNNNNGG 2 cut(s) 41, 554
BsmAI GTCTC 1 cut(s) 1014
BsmFI GGGAC 3 cut(s) 224, 764, 997
BsoBI CYCGRG 1 cut(s) 1015
Bsp119I TTCGAA 2 cut(s) 157, 218
Bsp1286I GDGCHC 1 cut(s) 337
Bsp143I GATC 2 cut(s) 889, 974
BspACI CCGC 1 cut(s) 826
BspCNI CTCAG 1 cut(s) 122
BspDI ATCGAT 1 cut(s) 977
BspLI GGNNCC 2 cut(s) 202, 526
BspPI GGATC 1 cut(s) 884
BspT104I TTCGAA 2 cut(s) 157, 218
BspT107I GGYRCC 1 cut(s) 524
BsrI ACTGG 7 cut(s) 76, 99, 117, 164, 556, 927, 1005
BssECI CCNNGG 1 cut(s) 376
BssMI GATC 2 cut(s) 889, 974
BssT1I CCWWGG 1 cut(s) 376
Bst2UI CCWGG 2 cut(s) 183, 230
Bst4CI ACNGT 1 cut(s) 920
Bst6I CTCTTC 2 cut(s) 15, 383
BstBI TTCGAA 2 cut(s) 157, 218
BstDEI CTNAG 1 cut(s) 109
BstF5I GGATG 2 cut(s) 31, 544
BstHHI GCGC 1 cut(s) 712
BstKTI GATC 2 cut(s) 892, 977
BstMAI GTCTC 1 cut(s) 1014
BstMBI GATC 2 cut(s) 889, 974
BstMWI GCNNNNNNNGC 1 cut(s) 538
BstNI CCWGG 2 cut(s) 183, 230
BstNSI RCATGY 1 cut(s) 817
BstSCI CCNGG 2 cut(s) 181, 228
BstV1I GCAGC 3 cut(s) 15, 163, 528
BstV2I GAAGAC 1 cut(s) 166
BstX2I RGATCY 1 cut(s) 889
BstYI RGATCY 1 cut(s) 889
Bsu15I ATCGAT 1 cut(s) 977
BsuTUI ATCGAT 1 cut(s) 977
BtgZI GCGATG 1 cut(s) 416
BtrI CACGTC 1 cut(s) 754
BtsCI GGATG 2 cut(s) 31, 544
BtsI GCAGTG 2 cut(s) 943, 971
BtsIMutI CAGTG 5 cut(s) 804, 826, 943, 971, 1012
CfoI GCGC 1 cut(s) 712
Cfr13I GGNCC 1 cut(s) 692
ClaI ATCGAT 1 cut(s) 977
Csp6I GTAC 4 cut(s) 297, 341, 511, 525
CviAII CATG 3 cut(s) 459, 514, 814
CviQI GTAC 4 cut(s) 297, 341, 511, 525
DdeI CTNAG 1 cut(s) 109
DpnI GATC 2 cut(s) 891, 976
DpnII GATC 2 cut(s) 889, 974
Eam1104I CTCTTC 2 cut(s) 15, 383
EarI CTCTTC 2 cut(s) 15, 383
Ecl136II GAGCTC 1 cut(s) 335
Eco130I CCWWGG 1 cut(s) 376
Eco24I GRGCYC 1 cut(s) 337
Eco47I GGWCC 1 cut(s) 692
Eco53kI GAGCTC 1 cut(s) 335
Eco57I CTGAAG 1 cut(s) 258
Eco88I CYCGRG 1 cut(s) 1015
EcoICRI GAGCTC 1 cut(s) 335
EcoO109I RGGNCCY 1 cut(s) 692
EcoRII CCWGG 2 cut(s) 181, 228
EcoT14I CCWWGG 1 cut(s) 376
EcoT38I GRGCYC 1 cut(s) 337
ErhI CCWWGG 1 cut(s) 376
FaeI CATG 3 cut(s) 462, 517, 817
FalI AAGNNNNNCTT 2 cut(s) 309, 341
FaqI GGGAC 3 cut(s) 224, 764, 997
FatI CATG 3 cut(s) 458, 513, 813
FauNDI CATATG 1 cut(s) 745
Fnu4HI GCNGC 3 cut(s) 29, 177, 542
FokI GGATG 2 cut(s) 18, 531
FriOI GRGCYC 1 cut(s) 337
Fsp4HI GCNGC 3 cut(s) 29, 177, 542
FspBI CTAG 1 cut(s) 626
GlaI GCGC 1 cut(s) 711
GluI GCNGC 3 cut(s) 29, 177, 542
GsaI CCCAGC 1 cut(s) 79
HapII CCGG 1 cut(s) 603
HhaI GCGC 1 cut(s) 712
Hin1II CATG 3 cut(s) 462, 517, 817
Hin6I GCGC 1 cut(s) 710
HinP1I GCGC 1 cut(s) 710
HinfI GANTC 2 cut(s) 769, 1010
HpaII CCGG 1 cut(s) 603
Hpy166II GTNNAC 2 cut(s) 789, 1051
Hpy188I TCNGA 6 cut(s) 85, 112, 277, 616, 955, 1064
Hpy188III TCNNGA 2 cut(s) 326, 878
Hpy8I GTNNAC 2 cut(s) 789, 1051
HpyAV CCTTC 2 cut(s) 349, 845
HpyCH4III ACNGT 1 cut(s) 920
HpyCH4IV ACGT 2 cut(s) 654, 753
HpyCH4V TGCA 6 cut(s) 31, 179, 532, 544, 887, 936
HpyF10VI GCNNNNNNNGC 1 cut(s) 538
HpyF3I CTNAG 1 cut(s) 109
HpySE526I ACGT 2 cut(s) 654, 753
Hsp92II CATG 3 cut(s) 462, 517, 817
HspAI GCGC 1 cut(s) 710
KpnI GGTACC 1 cut(s) 528
Kzo9I GATC 2 cut(s) 889, 974
Lsp1109I GCAGC 3 cut(s) 15, 163, 528
LweI GCATC 3 cut(s) 40, 439, 553
MaeI CTAG 1 cut(s) 626
MaeII ACGT 2 cut(s) 654, 753
MaeIII GTNAC 1 cut(s) 821
MalI GATC 2 cut(s) 891, 976
MboI GATC 2 cut(s) 889, 974
MboII GAAGA 6 cut(s) 32, 171, 217, 264, 400, 896
MflI RGATCY 1 cut(s) 889
MhlI GDGCHC 1 cut(s) 337
MluCI AATT 8 cut(s) 36, 214, 280, 842, 944, 957, 987, 993
MlyI GAGTC 1 cut(s) 1019
MmeI TCCRAC 1 cut(s) 572
MnlI CCTC 7 cut(s) 191, 403, 466, 683, 859, 905, 933
MroXI GAANNNNTTC 2 cut(s) 224, 392
MseI TTAA 3 cut(s) 363, 444, 686
MspI CCGG 1 cut(s) 603
MspR9I CCNGG 2 cut(s) 183, 230
MvaI CCWGG 2 cut(s) 183, 230
MwoI GCNNNNNNNGC 1 cut(s) 538
NdeI CATATG 1 cut(s) 745
NdeII GATC 2 cut(s) 889, 974
NlaIII CATG 3 cut(s) 462, 517, 817
NlaIV GGNNCC 2 cut(s) 202, 526
NmuCI GTSAC 1 cut(s) 821
NspI RCATGY 1 cut(s) 817
NspV TTCGAA 2 cut(s) 157, 218
PciI ACATGT 1 cut(s) 813
PdmI GAANNNNTTC 2 cut(s) 224, 392
PfeI GAWTC 1 cut(s) 769
PflMI CCANNNNNTGG 2 cut(s) 41, 554
PkrI GCNGC 3 cut(s) 30, 178, 543
PleI GAGTC 1 cut(s) 1018
PpsI GAGTC 1 cut(s) 1018
PpuMI RGGWCCY 1 cut(s) 692
PscI ACATGT 1 cut(s) 813
PsiI TTATAA 1 cut(s) 452
Psp124BI GAGCTC 1 cut(s) 337
Psp1406I AACGTT 1 cut(s) 654
Psp5II RGGWCCY 1 cut(s) 692
Psp6I CCWGG 2 cut(s) 181, 228
PspFI CCCAGC 1 cut(s) 75
PspGI CCWGG 2 cut(s) 181, 228
PspN4I GGNNCC 2 cut(s) 202, 526
PspPI GGNCC 1 cut(s) 692
PspPPI RGGWCCY 1 cut(s) 692
PsuI RGATCY 1 cut(s) 889
RsaI GTAC 4 cut(s) 298, 342, 512, 526
RsaNI GTAC 4 cut(s) 297, 341, 511, 525
SacI GAGCTC 1 cut(s) 337
SaqAI TTAA 3 cut(s) 363, 444, 686
SatI GCNGC 3 cut(s) 29, 177, 542
Sau3AI GATC 2 cut(s) 889, 974
Sau96I GGNCC 1 cut(s) 692
ScaI AGTACT 1 cut(s) 342
SchI GAGTC 1 cut(s) 1019
ScrFI CCNGG 2 cut(s) 183, 230
SduI GDGCHC 1 cut(s) 337
SfaNI GCATC 3 cut(s) 40, 439, 553
SfuI TTCGAA 2 cut(s) 157, 218
SinI GGWCC 1 cut(s) 692
SmlI CTYRAG 2 cut(s) 336, 970
SmoI CTYRAG 2 cut(s) 336, 970
Sse9I AATT 8 cut(s) 36, 214, 280, 842, 944, 957, 987, 993
SsiI CCGC 1 cut(s) 826
SspI AATATT 1 cut(s) 572
SspMI CTAG 1 cut(s) 626
SstI GAGCTC 1 cut(s) 337
StyD4I CCNGG 2 cut(s) 181, 228
StyI CCWWGG 1 cut(s) 376
TaaI ACNGT 1 cut(s) 920
TaiI ACGT 2 cut(s) 657, 756
TaqI TCGA 6 cut(s) 157, 192, 218, 464, 864, 977
TasI AATT 8 cut(s) 36, 214, 280, 842, 944, 957, 987, 993
TatI WGTACW 2 cut(s) 340, 510
TfiI GAWTC 1 cut(s) 769
Tru1I TTAA 3 cut(s) 363, 444, 686
Tru9I TTAA 3 cut(s) 363, 444, 686
TscAI CASTG 5 cut(s) 811, 826, 943, 971, 1012
TseFI GTSAC 1 cut(s) 821
TseI GCWGC 3 cut(s) 28, 176, 541
Tsp45I GTSAC 1 cut(s) 821
TspDTI ATGAA 4 cut(s) 225, 385, 735, 827
TspRI CASTG 5 cut(s) 811, 826, 943, 971, 1012
Van91I CCANNNNNTGG 2 cut(s) 41, 554
VpaK11BI GGWCC 1 cut(s) 692
XapI RAATTY 3 cut(s) 280, 842, 944
XceI RCATGY 1 cut(s) 817
XmnI GAANNNNTTC 2 cut(s) 224, 392
XspI CTAG 1 cut(s) 626
ZrmI AGTACT 1 cut(s) 342
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.