Rroxscaffold_4G00299490

LRR receptor-like serine threonine-protein kinase At3g47570

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000004
OG0018146
Physical Location & Seq
Reverse (-)
19430826 .. 19433137
2312 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_4G00299490.1

Sequence Viewer

Length: 885 bp
ATGTTCAGCGATCGCTTGAACCTTCATAAATTTGTGAGTATGTCTTGCTTTGAGGGAGTATTTCCAGAGATTGCAGATCCAAGTCTGCTTCTTCAAGGAGGCAACAGTCCTCCCAGTAAATGCAGTGGGAAATTCAAATCGGAAATTGGGCAGTGTTTTAGATCGATATTTGAAATTGGAATTGTGTGTTCCAGTGAGTCCCCGAGAGACAGAATGGATATACATTATGTTGTATCTGAATTGAATTCCGTTAGGGATGACTTTCTGAATAGGCTACATAGTTATAGTCCTACTGTTGGTGTAAGGTCAAGATCATGCAGCAGCTGCATGGTTTGTCGACGCAGGATGAAGGCACCACCTGCGTGGAGGTCATGGTCAAACTGCGGCTACCGCCAGTATGGTACGGGAAGTGGAGAGCGAAAATACTGGGATGTTTACAGTTTTGGCATTCTCCTTTTAGAAATGTTTACCGGAAAACGACCCACAGATCTCATGAATATTCGTAATTTTGTGAAGATGGCTATTCCTGAACGAGTTACAGAAATTGCAGATTCAAAACTGCTTCAAAGAGGCACCAACGATACTCAAAAAAATGAGTGCTTGATTTCGATATTTGAAATTGGAATTGCATGTTCAGTTGAAGCCCAGAGTAACCGAATGAGTATCGATCGTGTTTTGGAACATAAGCATTACGTGACCCAATTGTGCCTATTACATAAAATGGAAATTAGCTCTTCAGACAGTGTTGGGTCAAAGTTTGTTCAAGTAGGACAGGCAGACGGGCTCTTTTTGGGCTTTGATGGCCCAAAGAAAGAGTCACTCTTAAAGACGAACCCTAGGAAATCGAAGTTCTGGATTGTGTCTCCAACTGGGAGCTTCACATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRRNT_2 LRR_1 LRR_8 Pkinase Pkinase_Tyr

Protein Analysis

294

Amino Acids

33.21

Weight (kDa)

9.02

Isoelectric Point (pI)

58.79

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0018146)

Species Orthologous Gene IDs
rosa_chinensis RchiOBHm_Chr1g0356211 RchiOBHm_Chr1g0356491
rosa_laevigata RLG00000028092 RLG00000028093 RLG00000028099
rosa_multiflora Rmu_sc0000281.1_g000018 Rmu_sc0014206.1_g000003
rosa_roxburghii Rroxscaffold_4G00299490
rosa_rugosa Rorug01G0250100 Rorug01G0250100
rosa_wichuraiana Rw1G023310

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 367
Acc36I ACCTGC 1 cut(s) 367
AccB1I GGYRCC 2 cut(s) 352, 572
AccI GTMKAC 1 cut(s) 337
AciI CCGC 2 cut(s) 384, 391
AclWI GGATC 1 cut(s) 71
AcsI RAATTY 3 cut(s) 29, 131, 244
AcuI CTGAAG 1 cut(s) 720
AfaI GTAC 1 cut(s) 403
AfiI CCNNNNNNNGG 1 cut(s) 296
AleI CACNNNNGTG 1 cut(s) 361
AluBI AGCT 3 cut(s) 324, 732, 876
AluI AGCT 3 cut(s) 324, 732, 876
Alw26I GTCTC 2 cut(s) 201, 867
AlwI GGATC 1 cut(s) 71
AlwNI CAGNNNCTG 1 cut(s) 324
Ama87I CYCGRG 1 cut(s) 202
AoxI GGCC 1 cut(s) 802
ApeKI GCWGC 3 cut(s) 318, 321, 324
ApoI RAATTY 3 cut(s) 29, 131, 244
ArsI GACNNNNNNTTYG 2 cut(s) 800, 832
AsiSI GCGATCGC 1 cut(s) 13
AspA2I CCTAGG 1 cut(s) 836
AspS9I GGNCC 1 cut(s) 803
AvaI CYCGRG 1 cut(s) 202
AvrII CCTAGG 1 cut(s) 836
BanI GGYRCC 2 cut(s) 352, 572
BanII GRGCYC 1 cut(s) 786
BbvI GCAGC 3 cut(s) 311, 330, 333
BccI CCATC 2 cut(s) 511, 794
BcoDI GTCTC 2 cut(s) 201, 867
BfaI CTAG 1 cut(s) 837
BfuAI ACCTGC 1 cut(s) 367
BglII AGATCT 1 cut(s) 487
BisI GCNGC 4 cut(s) 319, 322, 325, 385
BlnI CCTAGG 1 cut(s) 836
BlsI GCNGC 4 cut(s) 320, 323, 326, 386
BmeT110I CYCGRG 1 cut(s) 202
BmgT120I GGNCC 1 cut(s) 803
BmiI GGNNCC 2 cut(s) 354, 574
BmrI ACTGGG 3 cut(s) 108, 436, 879
BmuI ACTGGG 3 cut(s) 108, 436, 879
Bsa29I ATCGAT 2 cut(s) 164, 666
BsaAI YACGTR 1 cut(s) 694
BsaJI CCNNGG 1 cut(s) 836
BsaWI WCCGGW 1 cut(s) 470
Bsc4I CCNNNNNNNGG 1 cut(s) 296
Bse1I ACTGG 5 cut(s) 114, 192, 394, 431, 874
BseCI ATCGAT 2 cut(s) 164, 666
BseDI CCNNGG 1 cut(s) 836
BseGI GGATG 3 cut(s) 262, 351, 436
BseLI CCNNNNNNNGG 1 cut(s) 296
BseNI ACTGG 5 cut(s) 114, 192, 394, 431, 874
BseXI GCAGC 3 cut(s) 311, 330, 333
Bsh1285I CGRYCG 2 cut(s) 13, 670
BshFI GGCC 1 cut(s) 804
BshNI GGYRCC 2 cut(s) 352, 572
BshVI ATCGAT 2 cut(s) 164, 666
BsiEI CGRYCG 2 cut(s) 13, 670
BsiHKCI CYCGRG 1 cut(s) 202
BsiSI CCGG 1 cut(s) 471
BslFI GGGAC 1 cut(s) 184
BslI CCNNNNNNNGG 1 cut(s) 296
BsmAI GTCTC 2 cut(s) 201, 867
BsmFI GGGAC 1 cut(s) 184
BsmI GAATGC 1 cut(s) 447
BsnI GGCC 1 cut(s) 804
BsoBI CYCGRG 1 cut(s) 202
Bsp1286I GDGCHC 1 cut(s) 786
Bsp143I GATC 6 cut(s) 10, 76, 161, 311, 487, 667
BspACI CCGC 2 cut(s) 384, 391
BspANI GGCC 1 cut(s) 804
BspDI ATCGAT 2 cut(s) 164, 666
BspHI TCATGA 1 cut(s) 492
BspLI GGNNCC 2 cut(s) 354, 574
BspMI ACCTGC 1 cut(s) 367
BspPI GGATC 1 cut(s) 71
BspQI GCTCTTC 1 cut(s) 739
BspT107I GGYRCC 2 cut(s) 352, 572
BsrI ACTGG 5 cut(s) 114, 192, 394, 431, 874
BssECI CCNNGG 1 cut(s) 836
BssMI GATC 6 cut(s) 10, 76, 161, 311, 487, 667
BssT1I CCWWGG 1 cut(s) 836
Bst4CI ACNGT 4 cut(s) 107, 295, 440, 743
Bst6I CTCTTC 1 cut(s) 739
BstAPI GCANNNNNTGC 2 cut(s) 324, 359
BstBAI YACGTR 1 cut(s) 694
BstF5I GGATG 3 cut(s) 262, 351, 436
BstKTI GATC 6 cut(s) 13, 79, 164, 314, 490, 670
BstMAI GTCTC 2 cut(s) 201, 867
BstMBI GATC 6 cut(s) 10, 76, 161, 311, 487, 667
BstMCI CGRYCG 2 cut(s) 13, 670
BstMWI GCNNNNNNNGC 4 cut(s) 324, 359, 390, 801
BstNSI RCATGY 1 cut(s) 633
BstV1I GCAGC 3 cut(s) 311, 330, 333
BstX2I RGATCY 2 cut(s) 76, 487
BstXI CCANNNNNNTGG 1 cut(s) 363
BstYI RGATCY 2 cut(s) 76, 487
Bsu15I ATCGAT 2 cut(s) 164, 666
BsuRI GGCC 1 cut(s) 804
BsuTUI ATCGAT 2 cut(s) 164, 666
BtsCI GGATG 3 cut(s) 262, 351, 436
BtsI GCAGTG 2 cut(s) 130, 158
BtsIMutI CAGTG 4 cut(s) 130, 158, 199, 748
BveI ACCTGC 1 cut(s) 367
CaiI CAGNNNCTG 1 cut(s) 324
CciI TCATGA 1 cut(s) 492
Cfr13I GGNCC 1 cut(s) 803
ClaI ATCGAT 2 cut(s) 164, 666
CseI GACGC 1 cut(s) 348
Csp6I GTAC 1 cut(s) 402
CviAII CATG 6 cut(s) 315, 328, 372, 493, 630, 882
CviQI GTAC 1 cut(s) 402
DpnI GATC 6 cut(s) 12, 78, 163, 313, 489, 669
DpnII GATC 6 cut(s) 10, 76, 161, 311, 487, 667
Eam1104I CTCTTC 1 cut(s) 739
EarI CTCTTC 1 cut(s) 739
Eco130I CCWWGG 1 cut(s) 836
Eco24I GRGCYC 1 cut(s) 786
Eco57I CTGAAG 1 cut(s) 720
Eco88I CYCGRG 1 cut(s) 202
EcoRI GAATTC 1 cut(s) 244
EcoT14I CCWWGG 1 cut(s) 836
EcoT38I GRGCYC 1 cut(s) 786
ErhI CCWWGG 1 cut(s) 836
FaeI CATG 6 cut(s) 318, 331, 375, 496, 633, 885
FaqI GGGAC 1 cut(s) 184
FatI CATG 6 cut(s) 314, 327, 371, 492, 629, 881
FblI GTMKAC 1 cut(s) 337
Fnu4HI GCNGC 4 cut(s) 319, 322, 325, 385
FokI GGATG 3 cut(s) 269, 358, 443
FriOI GRGCYC 1 cut(s) 786
Fsp4HI GCNGC 4 cut(s) 319, 322, 325, 385
FspBI CTAG 1 cut(s) 837
GluI GCNGC 4 cut(s) 319, 322, 325, 385
HaeIII GGCC 1 cut(s) 804
HapII CCGG 1 cut(s) 471
HgaI GACGC 1 cut(s) 348
Hin1II CATG 6 cut(s) 318, 331, 375, 496, 633, 885
HincII GTYRAC 1 cut(s) 338
HindII GTYRAC 1 cut(s) 338
HinfI GANTC 3 cut(s) 197, 551, 815
HpaII CCGG 1 cut(s) 471
Hpy166II GTNNAC 3 cut(s) 338, 436, 468
Hpy188I TCNGA 4 cut(s) 142, 238, 267, 739
Hpy188III TCNNGA 5 cut(s) 65, 309, 493, 527, 853
Hpy8I GTNNAC 3 cut(s) 338, 436, 468
Hpy99I CGWCG 1 cut(s) 342
HpyAV CCTTC 2 cut(s) 32, 343
HpyCH4III ACNGT 4 cut(s) 107, 295, 440, 743
HpyCH4IV ACGT 1 cut(s) 693
HpyCH4V TGCA 6 cut(s) 74, 123, 318, 327, 548, 629
HpyF10VI GCNNNNNNNGC 4 cut(s) 324, 359, 390, 801
HpySE526I ACGT 1 cut(s) 693
Hsp92II CATG 6 cut(s) 318, 331, 375, 496, 633, 885
Kzo9I GATC 6 cut(s) 10, 76, 161, 311, 487, 667
LguI GCTCTTC 1 cut(s) 739
LmnI GCTCC 1 cut(s) 873
Lsp1109I GCAGC 3 cut(s) 311, 330, 333
MaeI CTAG 1 cut(s) 837
MaeII ACGT 1 cut(s) 693
MaeIII GTNAC 4 cut(s) 535, 650, 694, 816
MalI GATC 6 cut(s) 12, 78, 163, 313, 489, 669
MboI GATC 6 cut(s) 10, 76, 161, 311, 487, 667
MboII GAAGA 3 cut(s) 83, 526, 726
MfeI CAATTG 1 cut(s) 701
MflI RGATCY 2 cut(s) 76, 487
MhlI GDGCHC 1 cut(s) 786
MlyI GAGTC 2 cut(s) 206, 824
MnlI CCTC 5 cut(s) 46, 92, 120, 360, 563
MseI TTAA 1 cut(s) 824
MslI CAYNNNNRTG 1 cut(s) 361
MspA1I CMGCKG 1 cut(s) 324
MspI CCGG 1 cut(s) 471
MunI CAATTG 1 cut(s) 701
Mva1269I GAATGC 1 cut(s) 447
MwoI GCNNNNNNNGC 4 cut(s) 324, 359, 390, 801
NdeII GATC 6 cut(s) 10, 76, 161, 311, 487, 667
NlaIII CATG 6 cut(s) 318, 331, 375, 496, 633, 885
NlaIV GGNNCC 2 cut(s) 354, 574
NmuCI GTSAC 2 cut(s) 694, 816
NspI RCATGY 1 cut(s) 633
OliI CACNNNNGTG 1 cut(s) 361
PagI TCATGA 1 cut(s) 492
PaqCI CACCTGC 1 cut(s) 367
PciSI GCTCTTC 1 cut(s) 739
PctI GAATGC 1 cut(s) 447
PfeI GAWTC 1 cut(s) 551
PkrI GCNGC 4 cut(s) 320, 323, 326, 386
Ple19I CGATCG 2 cut(s) 13, 670
PleI GAGTC 2 cut(s) 205, 823
PpsI GAGTC 2 cut(s) 205, 823
Ppu21I YACGTR 1 cut(s) 694
PspN4I GGNNCC 2 cut(s) 354, 574
PspPI GGNCC 1 cut(s) 803
PstNI CAGNNNCTG 1 cut(s) 324
PsuI RGATCY 2 cut(s) 76, 487
PvuI CGATCG 2 cut(s) 13, 670
PvuII CAGCTG 1 cut(s) 324
RgaI GCGATCGC 1 cut(s) 13
RsaI GTAC 1 cut(s) 403
RsaNI GTAC 1 cut(s) 402
RseI CAYNNNNRTG 1 cut(s) 361
SalI GTCGAC 1 cut(s) 336
SapI GCTCTTC 1 cut(s) 739
SaqAI TTAA 1 cut(s) 824
SatI GCNGC 4 cut(s) 319, 322, 325, 385
Sau3AI GATC 6 cut(s) 10, 76, 161, 311, 487, 667
Sau96I GGNCC 1 cut(s) 803
SchI GAGTC 2 cut(s) 206, 824
SduI GDGCHC 1 cut(s) 786
SetI ASST 8 cut(s) 24, 308, 326, 361, 371, 696, 734, 878
SfaAI GCGATCGC 1 cut(s) 13
SgfI GCGATCGC 1 cut(s) 13
SmiMI CAYNNNNRTG 1 cut(s) 361
SsiI CCGC 2 cut(s) 384, 391
SspI AATATT 1 cut(s) 499
SspMI CTAG 1 cut(s) 837
StyI CCWWGG 1 cut(s) 836
TaaI ACNGT 4 cut(s) 107, 295, 440, 743
TaiI ACGT 1 cut(s) 696
TaqI TCGA 5 cut(s) 164, 337, 608, 666, 845
TauI GCSGC 1 cut(s) 387
TfiI GAWTC 1 cut(s) 551
Tru1I TTAA 1 cut(s) 824
Tru9I TTAA 1 cut(s) 824
TscAI CASTG 4 cut(s) 130, 158, 199, 748
TseFI GTSAC 2 cut(s) 694, 816
TseI GCWGC 3 cut(s) 318, 321, 324
Tsp45I GTSAC 2 cut(s) 694, 816
TspDTI ATGAA 3 cut(s) 14, 362, 509
TspGWI ACGGA 1 cut(s) 238
TspRI CASTG 4 cut(s) 130, 158, 199, 748
XapI RAATTY 3 cut(s) 29, 131, 244
XceI RCATGY 1 cut(s) 633
XmaJI CCTAGG 1 cut(s) 836
XmiI GTMKAC 1 cut(s) 337
XspI CTAG 1 cut(s) 837
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.