RchiOBHm_Chr2g0098031

Belongs to the 'GDSL' lipolytic enzyme family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
2
OG0021341
Physical Location & Seq
Forward (+)
10513059 .. 10519046
5988 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ47285

Sequence Viewer

Length: 1359 bp
ATGTTGGCACCAATACCTTGTTGCTCCAAAGTGGAGCCAGGGCTGATTTCTCTCCAAATATATGGGGTTGATTTTCCTTATTCTGTGCCTACTGGGACGTTCAGCAATGGCCTTAACAGCGCTGATCAAATCGGTACAACCATCACAACTTCCTCTTCTTCAAGCTTAAGAAAGCTGCACAATATCATCAATGGCCTGGTTCAGCAGCACATTAAGGACCAGCCCCTCAACTTCCACACAGATCTCTTCGATTTCTTCTCGAAGAGCTCCTCGGCCTTACACCCTCGTGGATTAGCAGGGTTGTGTCGCCACCGTCATCGACGATCAGATCTGGGCCTCCGCTGGGACCCCAGTGCAGATCGCGCCTCCGATCTCCAGCTCCCGGTGTACGGCATCACGGCGATTCTGAACCTCTCGCTCTGCGACGAGAACAAGGAGCTCATAGCTTCGTCAGGAAATCCAACCACGAAAGAGAACGCTGCGTGCGCTCTACTCTGTCTATCTCAAATAGAAGTCGGAGGAGGAGAGGGACTAGGATTGGCTGGAGAGAGAGGCAGAGAGGTCGTCATTGGTGGAGGAGGTGAGACTGTGAGAGCGGTGTCAGGCTTGAGCGAGGCGGAGATACCTGCGGGAGCAAATATGAGGGTAGAGGTTTTGGATATTGGGTCAGTGGCAATGCCCGTAAATTGTGATGAAACTCGAGCATTTTGGTCATTTTTCATTAAAATTGGGAGTCAGGCTTGCATCATTTGGTTTTTGGGCCTGAGCTCATTGAAACTATTTGGATGCCGGAAAAGTCCACCATCTTTCCTGTATGCTGCGAGTCATATGTCCTCTTTTAAAAGGGATATACTGCAGGGAGTCAACTTTGCTTCAGCAGGAAGCGGCATCTTCCAAGACACGGGAATAAAAAGATGGACAGAAGTTGTGTCACTGGGAAATCAGATCCAACAATTTGCAGCGGTGCGTGGAAATTTCACAGAGATCGTTGGTTTCAAAACAACTGATACCGTGCTTTCCAAGTCTCTGTTCATCATCAGTATCGGAAGCAACGATCTTTTTGAATTAGTTGAGTACTTTCCAAATGTCATGGACTTGTATAAGGCAAAACTCATGGAACATCTTCAGCTAACGTACAAGAATCATTTGAAGAATTTATACAAGCTGGGAGCTCGGAAATTCGGGATTATAAGTGTTCCTCCAATTGGATGTTGTCCATATGCGCGGTTTGAGGACGTTCAATCAGCCTGCTGTGGAAACGGGAGGTTAAATGGAGAAAAAGCATGCGTTACAATTTTTGACCCCAACCTTTGTTCGAATCGTCATGATCCTATTCTGGGACTTGTATCATCCTACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lipase_GDSL

Protein Analysis

452

Amino Acids

49.0

Weight (kDa)

7.5

Isoelectric Point (pI)

40.43

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lipase_GDSL PF00657 282 - 412 1.4e-10 GDSL-like Lipase/Acylhydrolase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0021341)

Species Orthologous Gene IDs
rosa_chinensis RchiOBHm_Chr2g0098031
rosa_laevigata RLG00000011631 RLG00000012181
rosa_samantha Rh3BG285300 Rh7BG156700

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1190
Acc36I ACCTGC 1 cut(s) 634
AccB1I GGYRCC 1 cut(s) 7
AccBSI CCGCTC 1 cut(s) 596
AccII CGCG 2 cut(s) 363, 1225
AciI CCGC 7 cut(s) 340, 596, 617, 629, 885, 962, 1225
AclWI GGATC 2 cut(s) 940, 1322
AcsI RAATTY 3 cut(s) 973, 1153, 1178
AcuI CTGAAG 2 cut(s) 858, 1109
AfaI GTAC 4 cut(s) 136, 389, 1076, 1136
AfeI AGCGCT 1 cut(s) 121
AfiI CCNNNNNNNGG 6 cut(s) 343, 382, 389, 901, 1205, 1337
AflII CTTAAG 1 cut(s) 166
AgsI TTSAA 6 cut(s) 162, 775, 997, 1064, 1150, 1241
AjnI CCWGG 2 cut(s) 37, 195
AjuI GAANNNNNNNTTGG 2 cut(s) 1013, 1045
AleI CACNNNNGTG 1 cut(s) 285
Alw21I GWGCWC 4 cut(s) 269, 441, 770, 1174
Alw26I GTCTC 2 cut(s) 578, 1029
AlwI GGATC 2 cut(s) 940, 1322
Ama87I CYCGRG 1 cut(s) 699
Aor51HI AGCGCT 1 cut(s) 121
AoxI GGCC 5 cut(s) 109, 193, 273, 334, 760
ApeKI GCWGC 5 cut(s) 175, 205, 479, 818, 959
ApoI RAATTY 3 cut(s) 973, 1153, 1178
Asp700I GAANNNNTTC 1 cut(s) 1122
AspLEI GCGC 4 cut(s) 122, 365, 488, 1225
AspS9I GGNCC 4 cut(s) 217, 334, 346, 760
AsuC2I CCSGG 1 cut(s) 383
AsuHPI GGTGA 1 cut(s) 593
AsuII TTCGAA 1 cut(s) 1316
AvaI CYCGRG 1 cut(s) 699
AvaII GGWCC 2 cut(s) 217, 346
BanI GGYRCC 1 cut(s) 7
BanII GRGCYC 4 cut(s) 269, 441, 770, 1174
BarI GAAGNNNNNNTAC 2 cut(s) 1142, 1174
BauI CACGAG 1 cut(s) 285
Bbv12I GWGCWC 4 cut(s) 269, 441, 770, 1174
BbvI GCAGC 5 cut(s) 162, 217, 466, 805, 971
BccI CCATC 3 cut(s) 149, 811, 909
BceAI ACGGC 2 cut(s) 406, 414
BcgI CGANNNNNNTGC 2 cut(s) 544, 578
BciT130I CCWGG 2 cut(s) 39, 197
BclI TGATCA 1 cut(s) 124
BcnI CCSGG 1 cut(s) 383
BcoDI GTCTC 2 cut(s) 578, 1029
BfaI CTAG 1 cut(s) 533
BfmI CTRYAG 1 cut(s) 854
BfoI RGCGCY 1 cut(s) 123
BfrI CTTAAG 1 cut(s) 166
BfuAI ACCTGC 1 cut(s) 634
BglII AGATCT 2 cut(s) 241, 328
BisI GCNGC 6 cut(s) 176, 206, 480, 819, 886, 960
BlsI GCNGC 6 cut(s) 177, 207, 481, 820, 887, 961
BmcAI AGTACT 1 cut(s) 1076
Bme1390I CCNGG 3 cut(s) 39, 197, 383
Bme18I GGWCC 2 cut(s) 217, 346
BmeT110I CYCGRG 1 cut(s) 699
BmgT120I GGNCC 4 cut(s) 217, 334, 346, 760
BmiI GGNNCC 4 cut(s) 9, 36, 347, 348
BmrFI CCNGG 3 cut(s) 39, 197, 383
BmrI ACTGGG 3 cut(s) 102, 345, 944
BmsI GCATC 4 cut(s) 402, 753, 776, 897
BmuI ACTGGG 3 cut(s) 102, 345, 944
BpmI CTGGAG 2 cut(s) 359, 564
Bpu10I CCTNAGC 1 cut(s) 764
Bpu14I TTCGAA 1 cut(s) 1316
BpuEI CTTGAG 1 cut(s) 628
BpuMI CCSGG 1 cut(s) 383
BsaJI CCNNGG 2 cut(s) 38, 270
Bsc4I CCNNNNNNNGG 6 cut(s) 343, 382, 389, 901, 1205, 1337
Bse1I ACTGG 3 cut(s) 97, 351, 939
Bse3DI GCAATG 2 cut(s) 112, 681
BseBI CCWGG 2 cut(s) 39, 197
BseDI CCNNGG 2 cut(s) 38, 270
BseGI GGATG 3 cut(s) 791, 1214, 1349
BseLI CCNNNNNNNGG 6 cut(s) 343, 382, 389, 901, 1205, 1337
BseMI GCAATG 2 cut(s) 112, 681
BseMII CTCAG 1 cut(s) 755
BseNI ACTGG 3 cut(s) 97, 351, 939
BseRI GAGGAG 4 cut(s) 259, 534, 537, 591
BseXI GCAGC 5 cut(s) 162, 217, 466, 805, 971
BseYI CCCAGC 2 cut(s) 342, 1165
BsgI GTGCAG 2 cut(s) 161, 375
Bsh1236I CGCG 2 cut(s) 363, 1225
BshFI GGCC 5 cut(s) 111, 195, 275, 336, 762
BshNI GGYRCC 1 cut(s) 7
BsiHKAI GWGCWC 4 cut(s) 269, 441, 770, 1174
BsiHKCI CYCGRG 1 cut(s) 699
BsiSI CCGG 2 cut(s) 383, 790
BslFI GGGAC 4 cut(s) 109, 359, 543, 1353
BslI CCNNNNNNNGG 6 cut(s) 343, 382, 389, 901, 1205, 1337
BsmAI GTCTC 2 cut(s) 578, 1029
BsmFI GGGAC 4 cut(s) 109, 359, 543, 1353
BsnI GGCC 5 cut(s) 111, 195, 275, 336, 762
BsoBI CYCGRG 1 cut(s) 699
Bsp119I TTCGAA 1 cut(s) 1316
Bsp1286I GDGCHC 4 cut(s) 269, 441, 770, 1174
BspACI CCGC 7 cut(s) 340, 596, 617, 629, 885, 962, 1225
BspANI GGCC 5 cut(s) 111, 195, 275, 336, 762
BspCNI CTCAG 1 cut(s) 756
BspFNI CGCG 2 cut(s) 363, 1225
BspHI TCATGA 1 cut(s) 1324
BspLI GGNNCC 4 cut(s) 9, 36, 347, 348
BspMAI CTGCAG 1 cut(s) 858
BspMI ACCTGC 1 cut(s) 634
BspPI GGATC 2 cut(s) 940, 1322
BspQI GCTCTTC 1 cut(s) 257
BspT104I TTCGAA 1 cut(s) 1316
BspT107I GGYRCC 1 cut(s) 7
BspTI CTTAAG 1 cut(s) 166
BsrBI CCGCTC 1 cut(s) 596
BsrDI GCAATG 2 cut(s) 112, 681
BsrI ACTGG 3 cut(s) 97, 351, 939
BssECI CCNNGG 2 cut(s) 38, 270
BssSI CACGAG 1 cut(s) 285
Bst2BI CACGAG 1 cut(s) 285
Bst2UI CCWGG 2 cut(s) 39, 197
Bst4CI ACNGT 3 cut(s) 314, 589, 1012
Bst6I CTCTTC 3 cut(s) 160, 251, 257
BstAFI CTTAAG 1 cut(s) 166
BstBI TTCGAA 1 cut(s) 1316
BstC8I GCNNGC 4 cut(s) 484, 742, 1249, 1285
BstDEI CTNAG 1 cut(s) 764
BstF5I GGATG 3 cut(s) 791, 1214, 1349
BstFNI CGCG 2 cut(s) 363, 1225
BstH2I RGCGCY 1 cut(s) 123
BstHHI GCGC 4 cut(s) 122, 365, 488, 1225
BstMAI GTCTC 2 cut(s) 578, 1029
BstMWI GCNNNNNNNGC 3 cut(s) 117, 362, 485
BstNI CCWGG 2 cut(s) 39, 197
BstNSI RCATGY 1 cut(s) 1287
BstSCI CCNGG 3 cut(s) 37, 195, 381
BstSFI CTRYAG 1 cut(s) 854
BstUI CGCG 2 cut(s) 363, 1225
BstV1I GCAGC 5 cut(s) 162, 217, 466, 805, 971
BstX2I RGATCY 3 cut(s) 241, 328, 945
BstXI CCANNNNNNTGG 1 cut(s) 62
BstYI RGATCY 3 cut(s) 241, 328, 945
BsuRI GGCC 5 cut(s) 111, 195, 275, 336, 762
BtsCI GGATG 3 cut(s) 791, 1214, 1349
BtsIMutI CAGTG 3 cut(s) 358, 675, 932
BveI ACCTGC 1 cut(s) 634
Cac8I GCNNGC 4 cut(s) 484, 742, 1249, 1285
CciI TCATGA 1 cut(s) 1324
CfoI GCGC 4 cut(s) 122, 365, 488, 1225
Cfr13I GGNCC 4 cut(s) 217, 334, 346, 760
Csp6I GTAC 4 cut(s) 135, 388, 1075, 1135
CviAII CATG 4 cut(s) 1090, 1114, 1284, 1325
CviQI GTAC 4 cut(s) 135, 388, 1075, 1135
DdeI CTNAG 1 cut(s) 764
DraI TTTAAA 1 cut(s) 841
Eam1104I CTCTTC 3 cut(s) 160, 251, 257
EarI CTCTTC 3 cut(s) 160, 251, 257
EciI GGCGGA 1 cut(s) 632
Ecl136II GAGCTC 4 cut(s) 267, 439, 768, 1172
Eco24I GRGCYC 4 cut(s) 269, 441, 770, 1174
Eco47I GGWCC 2 cut(s) 217, 346
Eco47III AGCGCT 1 cut(s) 121
Eco53kI GAGCTC 4 cut(s) 267, 439, 768, 1172
Eco57I CTGAAG 2 cut(s) 858, 1109
Eco88I CYCGRG 1 cut(s) 699
EcoICRI GAGCTC 4 cut(s) 267, 439, 768, 1172
EcoO109I RGGNCCY 1 cut(s) 346
EcoRII CCWGG 2 cut(s) 37, 195
EcoT38I GRGCYC 4 cut(s) 269, 441, 770, 1174
FaeI CATG 4 cut(s) 1093, 1117, 1287, 1328
FaqI GGGAC 4 cut(s) 109, 359, 543, 1353
FatI CATG 4 cut(s) 1089, 1113, 1283, 1324
FauI CCCGC 1 cut(s) 622
FauNDI CATATG 2 cut(s) 828, 1219
FbaI TGATCA 1 cut(s) 124
Fnu4HI GCNGC 6 cut(s) 176, 206, 480, 819, 886, 960
FokI GGATG 3 cut(s) 798, 1221, 1336
FriOI GRGCYC 4 cut(s) 269, 441, 770, 1174
Fsp4HI GCNGC 6 cut(s) 176, 206, 480, 819, 886, 960
FspBI CTAG 1 cut(s) 533
GlaI GCGC 4 cut(s) 121, 364, 487, 1224
GluI GCNGC 6 cut(s) 176, 206, 480, 819, 886, 960
GsaI CCCAGC 2 cut(s) 346, 1169
GsuI CTGGAG 2 cut(s) 359, 564
HaeII RGCGCY 1 cut(s) 123
HaeIII GGCC 5 cut(s) 111, 195, 275, 336, 762
HapII CCGG 2 cut(s) 383, 790
HhaI GCGC 4 cut(s) 122, 365, 488, 1225
Hin1II CATG 4 cut(s) 1093, 1117, 1287, 1328
Hin6I GCGC 4 cut(s) 120, 363, 486, 1223
HinP1I GCGC 4 cut(s) 120, 363, 486, 1223
HincII GTYRAC 1 cut(s) 865
HindII GTYRAC 1 cut(s) 865
HindIII AAGCTT 1 cut(s) 163
HinfI GANTC 6 cut(s) 403, 733, 823, 861, 1141, 1318
HpaII CCGG 2 cut(s) 383, 790
HphI GGTGA 1 cut(s) 593
Hpy166II GTNNAC 3 cut(s) 388, 800, 865
Hpy188I TCNGA 7 cut(s) 328, 370, 408, 518, 945, 1046, 1176
Hpy188III TCNNGA 4 cut(s) 259, 453, 1183, 1325
Hpy8I GTNNAC 3 cut(s) 388, 800, 865
Hpy99I CGWCG 2 cut(s) 324, 428
HpyCH4III ACNGT 3 cut(s) 314, 589, 1012
HpyCH4IV ACGT 3 cut(s) 98, 1133, 1236
HpyCH4V TGCA 5 cut(s) 178, 356, 744, 856, 959
HpyF10VI GCNNNNNNNGC 3 cut(s) 117, 362, 485
HpyF3I CTNAG 1 cut(s) 764
HpySE526I ACGT 3 cut(s) 98, 1133, 1236
Hsp92II CATG 4 cut(s) 1093, 1117, 1287, 1328
HspAI GCGC 4 cut(s) 120, 363, 486, 1223
KflI GGGWCCC 1 cut(s) 346
Ksp22I TGATCA 1 cut(s) 124
LguI GCTCTTC 1 cut(s) 257
LmnI GCTCC 7 cut(s) 29, 34, 272, 384, 436, 632, 1169
Lsp1109I GCAGC 5 cut(s) 162, 217, 466, 805, 971
LweI GCATC 4 cut(s) 402, 753, 776, 897
MaeI CTAG 1 cut(s) 533
MaeII ACGT 3 cut(s) 98, 1133, 1236
MaeIII GTNAC 2 cut(s) 930, 1288
MbiI CCGCTC 1 cut(s) 596
MboII GAAGA 8 cut(s) 147, 150, 238, 247, 274, 883, 1115, 1162
MfeI CAATTG 1 cut(s) 1203
MflI RGATCY 3 cut(s) 241, 328, 945
MhlI GDGCHC 4 cut(s) 269, 441, 770, 1174
MluCI AATT 9 cut(s) 685, 726, 953, 973, 1064, 1153, 1178, 1203, 1293
MlyI GAGTC 3 cut(s) 742, 832, 870
MmeI TCCRAC 3 cut(s) 485, 496, 973
MroXI GAANNNNTTC 1 cut(s) 1122
MseI TTAA 6 cut(s) 114, 167, 213, 723, 840, 1268
MslI CAYNNNNRTG 1 cut(s) 285
MspA1I CMGCKG 2 cut(s) 342, 962
MspCI CTTAAG 1 cut(s) 166
MspI CCGG 2 cut(s) 383, 790
MspR9I CCNGG 3 cut(s) 39, 197, 383
MunI CAATTG 1 cut(s) 1203
MvaI CCWGG 2 cut(s) 39, 197
MvnI CGCG 2 cut(s) 363, 1225
MwoI GCNNNNNNNGC 3 cut(s) 117, 362, 485
NciI CCSGG 1 cut(s) 383
NdeI CATATG 2 cut(s) 828, 1219
NlaIII CATG 4 cut(s) 1093, 1117, 1287, 1328
NlaIV GGNNCC 4 cut(s) 9, 36, 347, 348
NmeAIII GCCGAG 1 cut(s) 251
NmuCI GTSAC 1 cut(s) 930
NspI RCATGY 1 cut(s) 1287
NspV TTCGAA 1 cut(s) 1316
OliI CACNNNNGTG 1 cut(s) 285
PaeI GCATGC 1 cut(s) 1287
PaeR7I CTCGAG 1 cut(s) 699
PagI TCATGA 1 cut(s) 1324
PciSI GCTCTTC 1 cut(s) 257
PcsI WCGNNNNNNNCGW 1 cut(s) 1050
PdmI GAANNNNTTC 1 cut(s) 1122
PfeI GAWTC 3 cut(s) 403, 1141, 1318
PkrI GCNGC 6 cut(s) 177, 207, 481, 820, 887, 961
PleI GAGTC 3 cut(s) 741, 831, 869
PpsI GAGTC 3 cut(s) 741, 831, 869
PpuMI RGGWCCY 1 cut(s) 346
PsiI TTATAA 1 cut(s) 1190
Psp124BI GAGCTC 4 cut(s) 269, 441, 770, 1174
Psp5II RGGWCCY 1 cut(s) 346
Psp6I CCWGG 2 cut(s) 37, 195
PspFI CCCAGC 2 cut(s) 342, 1165
PspGI CCWGG 2 cut(s) 37, 195
PspN4I GGNNCC 4 cut(s) 9, 36, 347, 348
PspPI GGNCC 4 cut(s) 217, 334, 346, 760
PspPPI RGGWCCY 1 cut(s) 346
PspXI VCTCGAGB 1 cut(s) 699
PstI CTGCAG 1 cut(s) 858
PsuI RGATCY 3 cut(s) 241, 328, 945
RsaI GTAC 4 cut(s) 136, 389, 1076, 1136
RsaNI GTAC 4 cut(s) 135, 388, 1075, 1135
RseI CAYNNNNRTG 1 cut(s) 285
SacI GAGCTC 4 cut(s) 269, 441, 770, 1174
SapI GCTCTTC 1 cut(s) 257
SaqAI TTAA 6 cut(s) 114, 167, 213, 723, 840, 1268
SatI GCNGC 6 cut(s) 176, 206, 480, 819, 886, 960
Sau96I GGNCC 4 cut(s) 217, 334, 346, 760
ScaI AGTACT 1 cut(s) 1076
SchI GAGTC 3 cut(s) 742, 832, 870
ScrFI CCNGG 3 cut(s) 39, 197, 383
SduI GDGCHC 4 cut(s) 269, 441, 770, 1174
SfaNI GCATC 4 cut(s) 402, 753, 776, 897
SfcI CTRYAG 1 cut(s) 854
Sfr274I CTCGAG 1 cut(s) 699
SfuI TTCGAA 1 cut(s) 1316
SinI GGWCC 2 cut(s) 217, 346
SlaI CTCGAG 1 cut(s) 699
SmiMI CAYNNNNRTG 1 cut(s) 285
SmlI CTYRAG 3 cut(s) 166, 607, 699
SmoI CTYRAG 3 cut(s) 166, 607, 699
SphI GCATGC 1 cut(s) 1287
Sse9I AATT 9 cut(s) 685, 726, 953, 973, 1064, 1153, 1178, 1203, 1293
SsiI CCGC 7 cut(s) 340, 596, 617, 629, 885, 962, 1225
SspMI CTAG 1 cut(s) 533
SstI GAGCTC 4 cut(s) 269, 441, 770, 1174
StyD4I CCNGG 3 cut(s) 37, 195, 381
TaaI ACNGT 3 cut(s) 314, 589, 1012
TaiI ACGT 3 cut(s) 101, 1136, 1239
TaqI TCGA 5 cut(s) 249, 260, 319, 700, 1316
TasI AATT 9 cut(s) 685, 726, 953, 973, 1064, 1153, 1178, 1203, 1293
TatI WGTACW 1 cut(s) 1074
TauI GCSGC 1 cut(s) 888
TfiI GAWTC 3 cut(s) 403, 1141, 1318
Tru1I TTAA 6 cut(s) 114, 167, 213, 723, 840, 1268
Tru9I TTAA 6 cut(s) 114, 167, 213, 723, 840, 1268
TscAI CASTG 3 cut(s) 358, 675, 939
TseFI GTSAC 1 cut(s) 930
TseI GCWGC 5 cut(s) 175, 205, 479, 818, 959
Tsp45I GTSAC 1 cut(s) 930
TspDTI ATGAA 3 cut(s) 708, 709, 1021
TspRI CASTG 3 cut(s) 358, 675, 939
Vha464I CTTAAG 1 cut(s) 166
VpaK11BI GGWCC 2 cut(s) 217, 346
XapI RAATTY 3 cut(s) 973, 1153, 1178
XceI RCATGY 1 cut(s) 1287
XhoI CTCGAG 1 cut(s) 699
XmnI GAANNNNTTC 1 cut(s) 1122
XspI CTAG 1 cut(s) 533
ZrmI AGTACT 1 cut(s) 1076
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.