RchiOBHm_Chr2g0137011

Belongs to the adaptor complexes large subunit family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
2
OG0019900
Physical Location & Seq
Forward (+)
54479766 .. 54480485
720 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ50779

Sequence Viewer

Length: 531 bp
ATGTTACCCTCAACCCAATTAAAGATTAATAATCACGCCATTCGACATCAATCACTCTCTCATCCTGATCTCTCCCCCTCTGGCGCTGTTTCTCTCACCTCGGTGCCGTCTCTCTCGCCTCCGGCGCTGTCTCTCTCTCCTTCACGCCGACTCTCTCGCCTCTGGCTCCGTCTCTCTCTCCTCCGCGCCGACTCTCTCTCGCCTCCGCAGGAAGCTCTCAAACGCCTCCTCGCTCTCATCGCCCATGGCTTCAATGTCTCTAATTTCTTCCCTCAGGTTGTTAAGAACGTAGCCACTCAGTCACTGGAGGTCAAGAGGCTAGTTTACTTGTACCTATTGCATTATGCTCAGAAGCGTCCGAATGAGGCTTTGCTATTGATTAATTGCTTCCAGAAGGATCTAGGGGATCCAAACCCATTGGTGATGGCATGGGCACTGCTAGCCATGGCCAGGATTAGGCTGCATGTTATTGCACCTTTGGTTGTGGTTGCTATGGGAAAATGTGCTAGAGATCCGTCTGTGTATGTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04142 map04142
Pfam Domains
Protein Families
AP3B1_C Adaptin_N

Protein Analysis

176

Amino Acids

19.48

Weight (kDa)

10.39

Isoelectric Point (pI)

56.04

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Adaptin_N PF01602 70 - 176 7.3e-27 Adaptin N terminal region
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0019900)

Species Orthologous Gene IDs
rosa_chinensis RchiOBHm_Chr2g0137011
rosa_laevigata RLG00000003950
rosa_multiflora Rmu_sc0003630.1_g000046
rosa_samantha Rh2AG386200 Rh2CG373500 Rh2DG409200 Rh5DG128900

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 103
AccII CGCG 1 cut(s) 186
AciI CCGC 2 cut(s) 184, 206
AclWI GGATC 4 cut(s) 401, 405, 414, 506
AcoI YGGCCR 1 cut(s) 447
AfaI GTAC 1 cut(s) 332
AfiI CCNNNNNNNGG 2 cut(s) 450, 456
AgsI TTSAA 1 cut(s) 253
AjnI CCWGG 1 cut(s) 449
AleI CACNNNNGTG 1 cut(s) 101
AluBI AGCT 1 cut(s) 215
AluI AGCT 1 cut(s) 215
Alw26I GTCTC 4 cut(s) 114, 135, 176, 262
AlwI GGATC 4 cut(s) 401, 405, 414, 506
AlwNI CAGNNNCTG 1 cut(s) 304
AoxI GGCC 1 cut(s) 447
ApeKI GCWGC 1 cut(s) 460
AseI ATTAAT 2 cut(s) 27, 381
AspLEI GCGC 3 cut(s) 86, 127, 188
AsuHPI GGTGA 2 cut(s) 88, 433
AsuNHI GCTAGC 1 cut(s) 439
AxyI CCTNAGG 1 cut(s) 273
BaeGI GKGCMC 1 cut(s) 436
BalI TGGCCA 1 cut(s) 449
BamHI GGATCC 1 cut(s) 406
BanI GGYRCC 1 cut(s) 103
BbvI GCAGC 1 cut(s) 447
BccI CCATC 1 cut(s) 418
BceAI ACGGC 1 cut(s) 91
BciT130I CCWGG 1 cut(s) 451
BcoDI GTCTC 4 cut(s) 114, 135, 176, 262
BfaI CTAG 4 cut(s) 320, 401, 440, 507
BfoI RGCGCY 2 cut(s) 87, 128
BisI GCNGC 1 cut(s) 461
BlsI GCNGC 1 cut(s) 462
Bme1390I CCNGG 1 cut(s) 451
BmiI GGNNCC 3 cut(s) 105, 167, 408
BmrFI CCNGG 1 cut(s) 451
BmtI GCTAGC 1 cut(s) 443
BpmI CTGGAG 1 cut(s) 326
BsaJI CCNNGG 3 cut(s) 99, 244, 444
Bsc4I CCNNNNNNNGG 2 cut(s) 450, 456
Bse1I ACTGG 1 cut(s) 309
Bse21I CCTNAGG 1 cut(s) 273
BseBI CCWGG 1 cut(s) 451
BseDI CCNNGG 3 cut(s) 99, 244, 444
BseGI GGATG 1 cut(s) 61
BseLI CCNNNNNNNGG 2 cut(s) 450, 456
BseMII CTCAG 3 cut(s) 287, 311, 362
BseNI ACTGG 1 cut(s) 309
BseRI GAGGAG 2 cut(s) 170, 218
BseSI GKGCMC 1 cut(s) 436
BseXI GCAGC 1 cut(s) 447
Bsh1236I CGCG 1 cut(s) 186
BshFI GGCC 1 cut(s) 449
BshNI GGYRCC 1 cut(s) 103
BsiSI CCGG 1 cut(s) 122
BslI CCNNNNNNNGG 2 cut(s) 450, 456
BsmAI GTCTC 4 cut(s) 114, 135, 176, 262
BsmBI CGTCTC 2 cut(s) 114, 176
BsnI GGCC 1 cut(s) 449
Bsp1286I GDGCHC 1 cut(s) 436
Bsp143I GATC 4 cut(s) 67, 397, 406, 511
Bsp19I CCATGG 2 cut(s) 244, 444
BspACI CCGC 2 cut(s) 184, 206
BspANI GGCC 1 cut(s) 449
BspCNI CTCAG 3 cut(s) 286, 310, 361
BspFNI CGCG 1 cut(s) 186
BspLI GGNNCC 3 cut(s) 105, 167, 408
BspOI GCTAGC 1 cut(s) 443
BspPI GGATC 4 cut(s) 401, 405, 414, 506
BspT107I GGYRCC 1 cut(s) 103
BsrI ACTGG 1 cut(s) 309
BssECI CCNNGG 3 cut(s) 99, 244, 444
BssMI GATC 4 cut(s) 67, 397, 406, 511
BssT1I CCWWGG 2 cut(s) 244, 444
Bst2UI CCWGG 1 cut(s) 451
BstC8I GCNNGC 1 cut(s) 441
BstDEI CTNAG 3 cut(s) 273, 297, 348
BstDSI CCRYGG 2 cut(s) 244, 444
BstF5I GGATG 1 cut(s) 61
BstFNI CGCG 1 cut(s) 186
BstH2I RGCGCY 2 cut(s) 87, 128
BstHHI GCGC 3 cut(s) 86, 127, 188
BstKTI GATC 4 cut(s) 70, 400, 409, 514
BstMAI GTCTC 4 cut(s) 114, 135, 176, 262
BstMBI GATC 4 cut(s) 67, 397, 406, 511
BstMWI GCNNNNNNNGC 3 cut(s) 124, 239, 440
BstNI CCWGG 1 cut(s) 451
BstNSI RCATGY 1 cut(s) 467
BstSCI CCNGG 1 cut(s) 449
BstSLI GKGCMC 1 cut(s) 436
BstUI CGCG 1 cut(s) 186
BstV1I GCAGC 1 cut(s) 447
BstX2I RGATCY 3 cut(s) 397, 406, 511
BstYI RGATCY 3 cut(s) 397, 406, 511
Bsu36I CCTNAGG 1 cut(s) 273
BsuRI GGCC 1 cut(s) 449
BtgI CCRYGG 2 cut(s) 244, 444
BtgZI GCGATG 1 cut(s) 223
BtsCI GGATG 1 cut(s) 61
BtsI GCAGTG 1 cut(s) 434
BtsIMutI CAGTG 2 cut(s) 302, 434
Cac8I GCNNGC 1 cut(s) 441
CaiI CAGNNNCTG 1 cut(s) 304
CfoI GCGC 3 cut(s) 86, 127, 188
CseI GACGC 1 cut(s) 344
Csp6I GTAC 1 cut(s) 331
CviAII CATG 4 cut(s) 245, 429, 445, 464
CviJI RGCY 9 cut(s) 166, 215, 249, 293, 319, 368, 443, 449, 460
CviKI_1 RGCY 9 cut(s) 166, 215, 249, 293, 319, 368, 443, 449, 460
CviQI GTAC 1 cut(s) 331
DdeI CTNAG 3 cut(s) 273, 297, 348
DpnI GATC 4 cut(s) 69, 399, 408, 513
DpnII GATC 4 cut(s) 67, 397, 406, 511
EaeI YGGCCR 1 cut(s) 447
Eco130I CCWWGG 2 cut(s) 244, 444
Eco81I CCTNAGG 1 cut(s) 273
EcoRII CCWGG 1 cut(s) 449
EcoT14I CCWWGG 2 cut(s) 244, 444
ErhI CCWWGG 2 cut(s) 244, 444
Esp3I CGTCTC 2 cut(s) 114, 176
FaeI CATG 4 cut(s) 248, 432, 448, 467
FaiI YATR 7 cut(s) 246, 345, 430, 446, 465, 494, 525
FatI CATG 4 cut(s) 244, 428, 444, 463
Fnu4HI GCNGC 1 cut(s) 461
FokI GGATG 1 cut(s) 48
Fsp4HI GCNGC 1 cut(s) 461
FspBI CTAG 4 cut(s) 320, 401, 440, 507
GlaI GCGC 3 cut(s) 85, 126, 187
GluI GCNGC 1 cut(s) 461
GsuI CTGGAG 1 cut(s) 326
HaeII RGCGCY 2 cut(s) 87, 128
HaeIII GGCC 1 cut(s) 449
HapII CCGG 1 cut(s) 122
HgaI GACGC 1 cut(s) 344
HhaI GCGC 3 cut(s) 86, 127, 188
Hin1II CATG 4 cut(s) 248, 432, 448, 467
Hin6I GCGC 3 cut(s) 84, 125, 186
HinP1I GCGC 3 cut(s) 84, 125, 186
HinfI GANTC 2 cut(s) 150, 191
HpaII CCGG 1 cut(s) 122
HphI GGTGA 2 cut(s) 88, 433
Hpy166II GTNNAC 1 cut(s) 325
Hpy188I TCNGA 2 cut(s) 351, 360
Hpy188III TCNNGA 3 cut(s) 65, 313, 391
Hpy8I GTNNAC 1 cut(s) 325
HpyAV CCTTC 2 cut(s) 150, 388
HpyCH4IV ACGT 1 cut(s) 288
HpyCH4V TGCA 3 cut(s) 340, 463, 473
HpyF10VI GCNNNNNNNGC 3 cut(s) 124, 239, 440
HpyF3I CTNAG 3 cut(s) 273, 297, 348
HpySE526I ACGT 1 cut(s) 288
Hsp92II CATG 4 cut(s) 248, 432, 448, 467
HspAI GCGC 3 cut(s) 84, 125, 186
Kzo9I GATC 4 cut(s) 67, 397, 406, 511
LmnI GCTCC 1 cut(s) 171
Lsp1109I GCAGC 1 cut(s) 447
MaeI CTAG 4 cut(s) 320, 401, 440, 507
MaeII ACGT 1 cut(s) 288
MaeIII GTNAC 2 cut(s) 3, 300
MalI GATC 4 cut(s) 69, 399, 408, 513
MboI GATC 4 cut(s) 67, 397, 406, 511
MboII GAAGA 1 cut(s) 259
MflI RGATCY 3 cut(s) 397, 406, 511
MhlI GDGCHC 1 cut(s) 436
MlsI TGGCCA 1 cut(s) 449
MluCI AATT 3 cut(s) 17, 262, 382
MluNI TGGCCA 1 cut(s) 449
MlyI GAGTC 2 cut(s) 144, 185
Mox20I TGGCCA 1 cut(s) 449
MscI TGGCCA 1 cut(s) 449
MseI TTAA 4 cut(s) 20, 27, 282, 381
MslI CAYNNNNRTG 1 cut(s) 101
Msp20I TGGCCA 1 cut(s) 449
MspI CCGG 1 cut(s) 122
MspR9I CCNGG 1 cut(s) 451
MvaI CCWGG 1 cut(s) 451
MvnI CGCG 1 cut(s) 186
MwoI GCNNNNNNNGC 3 cut(s) 124, 239, 440
NcoI CCATGG 2 cut(s) 244, 444
NdeII GATC 4 cut(s) 67, 397, 406, 511
NheI GCTAGC 1 cut(s) 439
NlaIII CATG 4 cut(s) 248, 432, 448, 467
NlaIV GGNNCC 3 cut(s) 105, 167, 408
NmuCI GTSAC 1 cut(s) 300
NspI RCATGY 1 cut(s) 467
OliI CACNNNNGTG 1 cut(s) 101
PkrI GCNGC 1 cut(s) 462
PleI GAGTC 2 cut(s) 144, 185
PpsI GAGTC 2 cut(s) 144, 185
PshBI ATTAAT 2 cut(s) 27, 381
Psp6I CCWGG 1 cut(s) 449
PspGI CCWGG 1 cut(s) 449
PspN4I GGNNCC 3 cut(s) 105, 167, 408
PstNI CAGNNNCTG 1 cut(s) 304
PsuI RGATCY 3 cut(s) 397, 406, 511
RsaI GTAC 1 cut(s) 332
RsaNI GTAC 1 cut(s) 331
RseI CAYNNNNRTG 1 cut(s) 101
SaqAI TTAA 4 cut(s) 20, 27, 282, 381
SatI GCNGC 1 cut(s) 461
Sau3AI GATC 4 cut(s) 67, 397, 406, 511
SchI GAGTC 2 cut(s) 144, 185
ScrFI CCNGG 1 cut(s) 451
SduI GDGCHC 1 cut(s) 436
SetI ASST 7 cut(s) 101, 217, 279, 291, 312, 336, 478
SmiMI CAYNNNNRTG 1 cut(s) 101
Sse9I AATT 3 cut(s) 17, 262, 382
SsiI CCGC 2 cut(s) 184, 206
SspMI CTAG 4 cut(s) 320, 401, 440, 507
StyD4I CCNGG 1 cut(s) 449
StyI CCWWGG 2 cut(s) 244, 444
TaiI ACGT 1 cut(s) 291
TaqI TCGA 1 cut(s) 43
TasI AATT 3 cut(s) 17, 262, 382
Tru1I TTAA 4 cut(s) 20, 27, 282, 381
Tru9I TTAA 4 cut(s) 20, 27, 282, 381
TscAI CASTG 2 cut(s) 309, 441
TseFI GTSAC 1 cut(s) 300
TseI GCWGC 1 cut(s) 460
Tsp45I GTSAC 1 cut(s) 300
TspGWI ACGGA 2 cut(s) 158, 504
TspRI CASTG 2 cut(s) 309, 441
VspI ATTAAT 2 cut(s) 27, 381
XceI RCATGY 1 cut(s) 467
XcmI CCANNNNNNNNNTGG 1 cut(s) 301
XspI CTAG 4 cut(s) 320, 401, 440, 507
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.