RLG00000003950

Belongs to the adaptor complexes large subunit family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr1
OG0019900
Physical Location & Seq
Forward (+)
54659540 .. 54660177
638 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000003950

Sequence Viewer

Length: 372 bp
ATGGCTTTGATGTCTCCAATTTCTTCCCTCAGGAATCCGAATGAGGCTTTGCTATTGATTAATTGCTTCCAGAAGGATCTAGGGGATCCAAACCCGTTGGTGAGGGCATGGGCACTGCTAGCCATGGCCGGGATTAGGCTGCATGTTATTGCACCTTTGGTTATGGTTGCTATGGGAAAATGTGCTAGAGATCCGTCTGTGTATGTTCGAAAATGTGCTGCCAATGCACTTACTAATCTAATGATTTGCGCCTTGATGAGTATACTGCCAGTATTGAAGAGGTCTGATTCATTTTTATGTTCAATAGTTGCAGACTTGTGGGAAATGGAAACAGTTGTGCATATATGTTCAATAGATCTTATTTGGTGGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04142 map04142
Pfam Domains
Protein Families
AP3B1_C Adaptin_N

Protein Analysis

124

Amino Acids

13.61

Weight (kDa)

7.6

Isoelectric Point (pI)

40.68

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Adaptin_N PF01602 11 - 81 1.4e-12 Adaptin N terminal region
Cnd1 PF12717 33 - 82 7.6e-06 non-SMC mitotic condensation complex subunit 1
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Orthologous Genes (Group: OG0019900)

Species Orthologous Gene IDs
rosa_chinensis RchiOBHm_Chr2g0137011
rosa_laevigata RLG00000003950
rosa_multiflora Rmu_sc0003630.1_g000046
rosa_samantha Rh2AG386200 Rh2CG373500 Rh2DG409200 Rh5DG128900

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 262
AclWI GGATC 4 cut(s) 80, 84, 93, 185
AcoI YGGCCR 1 cut(s) 126
AfiI CCNNNNNNNGG 2 cut(s) 129, 135
AgsI TTSAA 3 cut(s) 277, 303, 351
Alw26I GTCTC 1 cut(s) 18
AlwI GGATC 4 cut(s) 80, 84, 93, 185
AoxI GGCC 1 cut(s) 126
ApeKI GCWGC 2 cut(s) 139, 218
AseI ATTAAT 1 cut(s) 60
AspLEI GCGC 1 cut(s) 251
AsuC2I CCSGG 1 cut(s) 130
AsuHPI GGTGA 1 cut(s) 112
AsuII TTCGAA 1 cut(s) 208
AsuNHI GCTAGC 1 cut(s) 118
AxyI CCTNAGG 1 cut(s) 29
BaeGI GKGCMC 1 cut(s) 115
BamHI GGATCC 1 cut(s) 85
BbvI GCAGC 2 cut(s) 126, 205
BcnI CCSGG 1 cut(s) 130
BcoDI GTCTC 1 cut(s) 18
BfaI CTAG 3 cut(s) 80, 119, 186
BglII AGATCT 1 cut(s) 355
BisI GCNGC 2 cut(s) 140, 219
BlsI GCNGC 2 cut(s) 141, 220
Bme1390I CCNGG 1 cut(s) 130
BmiI GGNNCC 1 cut(s) 87
BmrFI CCNGG 1 cut(s) 130
BmtI GCTAGC 1 cut(s) 122
Bpu14I TTCGAA 1 cut(s) 208
BpuMI CCSGG 1 cut(s) 130
BsaJI CCNNGG 1 cut(s) 123
Bsc4I CCNNNNNNNGG 2 cut(s) 129, 135
Bse1I ACTGG 1 cut(s) 269
Bse21I CCTNAGG 1 cut(s) 29
BseDI CCNNGG 1 cut(s) 123
BseLI CCNNNNNNNGG 2 cut(s) 129, 135
BseMII CTCAG 1 cut(s) 43
BseNI ACTGG 1 cut(s) 269
BseSI GKGCMC 1 cut(s) 115
BseXI GCAGC 2 cut(s) 126, 205
BshFI GGCC 1 cut(s) 128
BsiSI CCGG 1 cut(s) 129
BslI CCNNNNNNNGG 2 cut(s) 129, 135
BsmAI GTCTC 1 cut(s) 18
BsnI GGCC 1 cut(s) 128
Bsp119I TTCGAA 1 cut(s) 208
Bsp1286I GDGCHC 1 cut(s) 115
Bsp143I GATC 4 cut(s) 76, 85, 190, 355
Bsp19I CCATGG 1 cut(s) 123
BspANI GGCC 1 cut(s) 128
BspCNI CTCAG 1 cut(s) 42
BspLI GGNNCC 1 cut(s) 87
BspOI GCTAGC 1 cut(s) 122
BspPI GGATC 4 cut(s) 80, 84, 93, 185
BspT104I TTCGAA 1 cut(s) 208
BsrI ACTGG 1 cut(s) 269
BssECI CCNNGG 1 cut(s) 123
BssMI GATC 4 cut(s) 76, 85, 190, 355
BssNAI GTATAC 1 cut(s) 263
BssT1I CCWWGG 1 cut(s) 123
Bst1107I GTATAC 1 cut(s) 263
Bst4CI ACNGT 1 cut(s) 334
Bst6I CTCTTC 1 cut(s) 272
BstBI TTCGAA 1 cut(s) 208
BstC8I GCNNGC 1 cut(s) 120
BstDEI CTNAG 1 cut(s) 29
BstDSI CCRYGG 1 cut(s) 123
BstHHI GCGC 1 cut(s) 251
BstKTI GATC 4 cut(s) 79, 88, 193, 358
BstMAI GTCTC 1 cut(s) 18
BstMBI GATC 4 cut(s) 76, 85, 190, 355
BstMWI GCNNNNNNNGC 2 cut(s) 119, 224
BstNSI RCATGY 1 cut(s) 146
BstSCI CCNGG 1 cut(s) 128
BstSLI GKGCMC 1 cut(s) 115
BstV1I GCAGC 2 cut(s) 126, 205
BstX2I RGATCY 4 cut(s) 76, 85, 190, 355
BstYI RGATCY 4 cut(s) 76, 85, 190, 355
BstZ17I GTATAC 1 cut(s) 263
Bsu36I CCTNAGG 1 cut(s) 29
BsuRI GGCC 1 cut(s) 128
BtgI CCRYGG 1 cut(s) 123
BtsI GCAGTG 1 cut(s) 113
BtsIMutI CAGTG 1 cut(s) 113
Cac8I GCNNGC 1 cut(s) 120
CfoI GCGC 1 cut(s) 251
CviAII CATG 3 cut(s) 108, 124, 143
CviJI RGCY 5 cut(s) 5, 47, 122, 128, 139
CviKI_1 RGCY 5 cut(s) 5, 47, 122, 128, 139
DdeI CTNAG 1 cut(s) 29
DpnI GATC 4 cut(s) 78, 87, 192, 357
DpnII GATC 4 cut(s) 76, 85, 190, 355
EaeI YGGCCR 1 cut(s) 126
Eam1104I CTCTTC 1 cut(s) 272
EarI CTCTTC 1 cut(s) 272
Eco130I CCWWGG 1 cut(s) 123
Eco81I CCTNAGG 1 cut(s) 29
EcoT14I CCWWGG 1 cut(s) 123
ErhI CCWWGG 1 cut(s) 123
FaeI CATG 3 cut(s) 111, 127, 146
FatI CATG 3 cut(s) 107, 123, 142
FblI GTMKAC 1 cut(s) 262
Fnu4HI GCNGC 2 cut(s) 140, 219
Fsp4HI GCNGC 2 cut(s) 140, 219
FspBI CTAG 3 cut(s) 80, 119, 186
GlaI GCGC 1 cut(s) 250
GluI GCNGC 2 cut(s) 140, 219
HaeIII GGCC 1 cut(s) 128
HapII CCGG 1 cut(s) 129
HhaI GCGC 1 cut(s) 251
Hin1II CATG 3 cut(s) 111, 127, 146
Hin6I GCGC 1 cut(s) 249
HinP1I GCGC 1 cut(s) 249
HinfI GANTC 2 cut(s) 34, 287
HpaII CCGG 1 cut(s) 129
HphI GGTGA 1 cut(s) 112
Hpy166II GTNNAC 1 cut(s) 263
Hpy188I TCNGA 2 cut(s) 39, 286
Hpy188III TCNNGA 2 cut(s) 31, 70
Hpy8I GTNNAC 1 cut(s) 263
HpyAV CCTTC 1 cut(s) 67
HpyCH4III ACNGT 1 cut(s) 334
HpyCH4V TGCA 5 cut(s) 142, 152, 227, 311, 340
HpyF10VI GCNNNNNNNGC 2 cut(s) 119, 224
HpyF3I CTNAG 1 cut(s) 29
Hsp92II CATG 3 cut(s) 111, 127, 146
HspAI GCGC 1 cut(s) 249
Kzo9I GATC 4 cut(s) 76, 85, 190, 355
LpnPI CCDG 4 cut(s) 16, 83, 142, 282
Lsp1109I GCAGC 2 cut(s) 126, 205
MaeI CTAG 3 cut(s) 80, 119, 186
MalI GATC 4 cut(s) 78, 87, 192, 357
MboI GATC 4 cut(s) 76, 85, 190, 355
MboII GAAGA 2 cut(s) 15, 289
MflI RGATCY 4 cut(s) 76, 85, 190, 355
MhlI GDGCHC 1 cut(s) 115
MluCI AATT 2 cut(s) 18, 61
MnlI CCTC 4 cut(s) 37, 38, 96, 273
MseI TTAA 1 cut(s) 60
MslI CAYNNNNRTG 1 cut(s) 295
MspI CCGG 1 cut(s) 129
MspR9I CCNGG 1 cut(s) 130
MwoI GCNNNNNNNGC 2 cut(s) 119, 224
NciI CCSGG 1 cut(s) 130
NcoI CCATGG 1 cut(s) 123
NdeII GATC 4 cut(s) 76, 85, 190, 355
NheI GCTAGC 1 cut(s) 118
NlaIII CATG 3 cut(s) 111, 127, 146
NlaIV GGNNCC 1 cut(s) 87
NspI RCATGY 1 cut(s) 146
NspV TTCGAA 1 cut(s) 208
PfeI GAWTC 2 cut(s) 34, 287
PkrI GCNGC 2 cut(s) 141, 220
PshBI ATTAAT 1 cut(s) 60
PspN4I GGNNCC 1 cut(s) 87
PsuI RGATCY 4 cut(s) 76, 85, 190, 355
RseI CAYNNNNRTG 1 cut(s) 295
SaqAI TTAA 1 cut(s) 60
SatI GCNGC 2 cut(s) 140, 219
Sau3AI GATC 4 cut(s) 76, 85, 190, 355
ScrFI CCNGG 1 cut(s) 130
SduI GDGCHC 1 cut(s) 115
SetI ASST 2 cut(s) 157, 284
SfuI TTCGAA 1 cut(s) 208
SmiMI CAYNNNNRTG 1 cut(s) 295
Sse9I AATT 2 cut(s) 18, 61
SspMI CTAG 3 cut(s) 80, 119, 186
StyD4I CCNGG 1 cut(s) 128
StyI CCWWGG 1 cut(s) 123
TaaI ACNGT 1 cut(s) 334
TaqI TCGA 1 cut(s) 208
TasI AATT 2 cut(s) 18, 61
TfiI GAWTC 2 cut(s) 34, 287
Tru1I TTAA 1 cut(s) 60
Tru9I TTAA 1 cut(s) 60
TscAI CASTG 1 cut(s) 120
TseI GCWGC 2 cut(s) 139, 218
TspDTI ATGAA 1 cut(s) 279
TspGWI ACGGA 1 cut(s) 183
TspRI CASTG 1 cut(s) 120
VspI ATTAAT 1 cut(s) 60
XceI RCATGY 1 cut(s) 146
XmiI GTMKAC 1 cut(s) 262
XspI CTAG 3 cut(s) 80, 119, 186
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.