Rh2AG386200

Belongs to the adaptor complexes large subunit family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2A
OG0019900
Physical Location & Seq
Forward (+)
57682609 .. 57711956
29348 bp
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UTR
Exon/CDS
Intron
Rh2AG386200.1

Sequence Viewer

Length: 360 bp
ATGAGCTTAAATAACTCAAGGCGTCCGAATGAGGCTTTGCTATTGATTAATTGCTTCCAGAAGGATCTAGGGGATCCAAACCCGTTGGTGAGGGCATGGGCACTGCTAGCCATGGCCGGGATTAGGCTGCATGTTATTGCACCTTTGGTTGTGGTTGCTGTGGGAAAATGTGCTAGAGATCCGTCTGTGTATGTTCGAAAATGTGCTGCCAATGCACTTACTAAGCTAATGATTTGCGCCTTGATGAGTATACTGCCAGTATTGAAGAGGTCTGATTCATTTTTATGTTCAATAGTTGCAGACTTGTGGGAAATGGAAACAGTTGTGCATATATGTTCAATAGATCTTATTTGGTGGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04142 map04142
Pfam Domains
Protein Families
AP3B1_C Adaptin_N

Protein Analysis

119

Amino Acids

13.22

Weight (kDa)

8.63

Isoelectric Point (pI)

40.84

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Adaptin_N PF01602 7 - 77 8e-13 Adaptin N terminal region
Cnd1 PF12717 29 - 79 1.8e-06 non-SMC mitotic condensation complex subunit 1
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0019900)

Species Orthologous Gene IDs
rosa_chinensis RchiOBHm_Chr2g0137011
rosa_laevigata RLG00000003950
rosa_multiflora Rmu_sc0003630.1_g000046
rosa_samantha Rh2AG386200 Rh2CG373500 Rh2DG409200 Rh5DG128900

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 250
AclWI GGATC 4 cut(s) 68, 72, 81, 173
AcoI YGGCCR 1 cut(s) 114
AcyI GRCGYC 1 cut(s) 22
AfiI CCNNNNNNNGG 2 cut(s) 117, 123
AgsI TTSAA 3 cut(s) 265, 291, 339
AluBI AGCT 2 cut(s) 6, 226
AluI AGCT 2 cut(s) 6, 226
AlwI GGATC 4 cut(s) 68, 72, 81, 173
AoxI GGCC 1 cut(s) 114
ApeKI GCWGC 2 cut(s) 127, 206
AseI ATTAAT 1 cut(s) 48
AspLEI GCGC 1 cut(s) 239
AsuC2I CCSGG 1 cut(s) 118
AsuHPI GGTGA 1 cut(s) 100
AsuII TTCGAA 1 cut(s) 196
AsuNHI GCTAGC 1 cut(s) 106
BaeGI GKGCMC 1 cut(s) 103
BamHI GGATCC 1 cut(s) 73
BbvI GCAGC 2 cut(s) 114, 193
BcnI CCSGG 1 cut(s) 118
BfaI CTAG 3 cut(s) 68, 107, 174
BglII AGATCT 1 cut(s) 343
BisI GCNGC 2 cut(s) 128, 207
BlsI GCNGC 2 cut(s) 129, 208
Bme1390I CCNGG 1 cut(s) 118
BmiI GGNNCC 1 cut(s) 75
BmrFI CCNGG 1 cut(s) 118
BmtI GCTAGC 1 cut(s) 110
Bpu14I TTCGAA 1 cut(s) 196
BpuMI CCSGG 1 cut(s) 118
BsaHI GRCGYC 1 cut(s) 22
BsaJI CCNNGG 1 cut(s) 111
Bsc4I CCNNNNNNNGG 2 cut(s) 117, 123
Bse1I ACTGG 1 cut(s) 257
BseDI CCNNGG 1 cut(s) 111
BseLI CCNNNNNNNGG 2 cut(s) 117, 123
BseNI ACTGG 1 cut(s) 257
BseSI GKGCMC 1 cut(s) 103
BseXI GCAGC 2 cut(s) 114, 193
BshFI GGCC 1 cut(s) 116
BsiSI CCGG 1 cut(s) 117
BslI CCNNNNNNNGG 2 cut(s) 117, 123
BsnI GGCC 1 cut(s) 116
Bsp119I TTCGAA 1 cut(s) 196
Bsp1286I GDGCHC 1 cut(s) 103
Bsp143I GATC 4 cut(s) 64, 73, 178, 343
Bsp19I CCATGG 1 cut(s) 111
BspANI GGCC 1 cut(s) 116
BspLI GGNNCC 1 cut(s) 75
BspOI GCTAGC 1 cut(s) 110
BspPI GGATC 4 cut(s) 68, 72, 81, 173
BspT104I TTCGAA 1 cut(s) 196
BsrI ACTGG 1 cut(s) 257
BssECI CCNNGG 1 cut(s) 111
BssMI GATC 4 cut(s) 64, 73, 178, 343
BssNAI GTATAC 1 cut(s) 251
BssNI GRCGYC 1 cut(s) 22
BssT1I CCWWGG 1 cut(s) 111
Bst1107I GTATAC 1 cut(s) 251
Bst4CI ACNGT 1 cut(s) 322
Bst6I CTCTTC 1 cut(s) 260
BstACI GRCGYC 1 cut(s) 22
BstBI TTCGAA 1 cut(s) 196
BstC8I GCNNGC 1 cut(s) 108
BstDEI CTNAG 1 cut(s) 222
BstDSI CCRYGG 1 cut(s) 111
BstHHI GCGC 1 cut(s) 239
BstKTI GATC 4 cut(s) 67, 76, 181, 346
BstMBI GATC 4 cut(s) 64, 73, 178, 343
BstMWI GCNNNNNNNGC 2 cut(s) 107, 212
BstNSI RCATGY 1 cut(s) 134
BstSCI CCNGG 1 cut(s) 116
BstSLI GKGCMC 1 cut(s) 103
BstV1I GCAGC 2 cut(s) 114, 193
BstX2I RGATCY 4 cut(s) 64, 73, 178, 343
BstYI RGATCY 4 cut(s) 64, 73, 178, 343
BstZ17I GTATAC 1 cut(s) 251
BsuRI GGCC 1 cut(s) 116
BtgI CCRYGG 1 cut(s) 111
BtsI GCAGTG 1 cut(s) 101
BtsIMutI CAGTG 1 cut(s) 101
Cac8I GCNNGC 1 cut(s) 108
CfoI GCGC 1 cut(s) 239
CseI GACGC 1 cut(s) 11
CviAII CATG 3 cut(s) 96, 112, 131
CviJI RGCY 6 cut(s) 6, 35, 110, 116, 127, 226
CviKI_1 RGCY 6 cut(s) 6, 35, 110, 116, 127, 226
DdeI CTNAG 1 cut(s) 222
DpnI GATC 4 cut(s) 66, 75, 180, 345
DpnII GATC 4 cut(s) 64, 73, 178, 343
EaeI YGGCCR 1 cut(s) 114
Eam1104I CTCTTC 1 cut(s) 260
EarI CTCTTC 1 cut(s) 260
Eco130I CCWWGG 1 cut(s) 111
EcoT14I CCWWGG 1 cut(s) 111
ErhI CCWWGG 1 cut(s) 111
FaeI CATG 3 cut(s) 99, 115, 134
FaiI YATR 9 cut(s) 97, 113, 132, 192, 251, 286, 330, 332, 334
FatI CATG 3 cut(s) 95, 111, 130
FblI GTMKAC 1 cut(s) 250
Fnu4HI GCNGC 2 cut(s) 128, 207
Fsp4HI GCNGC 2 cut(s) 128, 207
FspBI CTAG 3 cut(s) 68, 107, 174
GlaI GCGC 1 cut(s) 238
GluI GCNGC 2 cut(s) 128, 207
HaeIII GGCC 1 cut(s) 116
HapII CCGG 1 cut(s) 117
HgaI GACGC 1 cut(s) 11
HhaI GCGC 1 cut(s) 239
Hin1I GRCGYC 1 cut(s) 22
Hin1II CATG 3 cut(s) 99, 115, 134
Hin6I GCGC 1 cut(s) 237
HinP1I GCGC 1 cut(s) 237
HinfI GANTC 1 cut(s) 275
HpaII CCGG 1 cut(s) 117
HphI GGTGA 1 cut(s) 100
Hpy166II GTNNAC 1 cut(s) 251
Hpy188I TCNGA 2 cut(s) 27, 274
Hpy188III TCNNGA 1 cut(s) 58
Hpy8I GTNNAC 1 cut(s) 251
HpyAV CCTTC 1 cut(s) 55
HpyCH4III ACNGT 1 cut(s) 322
HpyCH4V TGCA 5 cut(s) 130, 140, 215, 299, 328
HpyF10VI GCNNNNNNNGC 2 cut(s) 107, 212
HpyF3I CTNAG 1 cut(s) 222
Hsp92I GRCGYC 1 cut(s) 22
Hsp92II CATG 3 cut(s) 99, 115, 134
HspAI GCGC 1 cut(s) 237
Kzo9I GATC 4 cut(s) 64, 73, 178, 343
LpnPI CCDG 3 cut(s) 71, 130, 270
Lsp1109I GCAGC 2 cut(s) 114, 193
MaeI CTAG 3 cut(s) 68, 107, 174
MalI GATC 4 cut(s) 66, 75, 180, 345
MboI GATC 4 cut(s) 64, 73, 178, 343
MboII GAAGA 1 cut(s) 277
MflI RGATCY 4 cut(s) 64, 73, 178, 343
MhlI GDGCHC 1 cut(s) 103
MluCI AATT 1 cut(s) 49
MnlI CCTC 3 cut(s) 25, 84, 261
MseI TTAA 2 cut(s) 8, 48
MslI CAYNNNNRTG 1 cut(s) 283
MspI CCGG 1 cut(s) 117
MspR9I CCNGG 1 cut(s) 118
MwoI GCNNNNNNNGC 2 cut(s) 107, 212
NciI CCSGG 1 cut(s) 118
NcoI CCATGG 1 cut(s) 111
NdeII GATC 4 cut(s) 64, 73, 178, 343
NheI GCTAGC 1 cut(s) 106
NlaIII CATG 3 cut(s) 99, 115, 134
NlaIV GGNNCC 1 cut(s) 75
NspI RCATGY 1 cut(s) 134
NspV TTCGAA 1 cut(s) 196
PfeI GAWTC 1 cut(s) 275
PkrI GCNGC 2 cut(s) 129, 208
PshBI ATTAAT 1 cut(s) 48
PspN4I GGNNCC 1 cut(s) 75
PsuI RGATCY 4 cut(s) 64, 73, 178, 343
RseI CAYNNNNRTG 1 cut(s) 283
SaqAI TTAA 2 cut(s) 8, 48
SatI GCNGC 2 cut(s) 128, 207
Sau3AI GATC 4 cut(s) 64, 73, 178, 343
ScrFI CCNGG 1 cut(s) 118
SduI GDGCHC 1 cut(s) 103
SetI ASST 4 cut(s) 8, 145, 228, 272
SfuI TTCGAA 1 cut(s) 196
SmiMI CAYNNNNRTG 1 cut(s) 283
SmlI CTYRAG 1 cut(s) 16
SmoI CTYRAG 1 cut(s) 16
Sse9I AATT 1 cut(s) 49
SspMI CTAG 3 cut(s) 68, 107, 174
StyD4I CCNGG 1 cut(s) 116
StyI CCWWGG 1 cut(s) 111
TaaI ACNGT 1 cut(s) 322
TaqI TCGA 1 cut(s) 196
TasI AATT 1 cut(s) 49
TfiI GAWTC 1 cut(s) 275
Tru1I TTAA 2 cut(s) 8, 48
Tru9I TTAA 2 cut(s) 8, 48
TscAI CASTG 1 cut(s) 108
TseI GCWGC 2 cut(s) 127, 206
TspDTI ATGAA 1 cut(s) 267
TspGWI ACGGA 1 cut(s) 171
TspRI CASTG 1 cut(s) 108
VspI ATTAAT 1 cut(s) 48
XceI RCATGY 1 cut(s) 134
XmiI GTMKAC 1 cut(s) 250
XspI CTAG 3 cut(s) 68, 107, 174
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.