RchiOBHm_Chr2g0145871

Cytochrome p450

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
2
OG0021147
Physical Location & Seq
Reverse (-)
63607514 .. 63607903
390 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ51569

Sequence Viewer

Length: 390 bp
ATGGAAGACACTACTTGGGTTCCCTACGCCACTGCATGGCTGGGAGCTCTAAACCTTATCCTGTTTTCCCGCCATCTCCGCCGCCGAAAACTCAATTTACCGCCCCGCCCAAAACCTTGGCCCATCATTGGAAACCTCAACCTCATAGGCCACCTCCCTCACCGTTCTGTCCATGCACTTTCCCAAATATATGGGCCCATCATGCAGCTGAAATTCGGGTCCTTCCCCATTGTCGTGGCCTCCTCGGTCGAAATGGCCGAGGCCTTCCTCAAAACTGACGACGTTACTTTGGCCGACCGGCCCAAAATGGCGGCGGGAAAGTACACCACCTACAACTACTCCGACATCACTTTGTCCCCTTATGGCCCATATTGGCGCCAGGGCCGTTAA

Protein Analysis

129

Amino Acids

14.69

Weight (kDa)

9.92

Isoelectric Point (pI)

40.99

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
p450 PF00067 34 - 129 9.1e-13 Cytochrome P450
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0021147)

Species Orthologous Gene IDs
malus_domestica MD15G1218300.v1.1
rosa_chinensis RchiOBHm_Chr2g0145871
rosa_laevigata RLG00000017227
rosa_samantha Rh2BG169500 Rh2DG469600

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 375
AccB7I CCANNNNNTGG 1 cut(s) 36
AciI CCGC 7 cut(s) 70, 79, 82, 101, 106, 311, 314
AcoI YGGCCR 2 cut(s) 255, 291
AcsI RAATTY 1 cut(s) 212
AcyI GRCGYC 1 cut(s) 376
AfaI GTAC 1 cut(s) 323
AfiI CCNNNNNNNGG 2 cut(s) 36, 128
AjnI CCWGG 1 cut(s) 378
AluBI AGCT 2 cut(s) 47, 208
AluI AGCT 2 cut(s) 47, 208
Alw21I GWGCWC 1 cut(s) 49
ApaI GGGCCC 1 cut(s) 198
ApeKI GCWGC 1 cut(s) 205
ApoI RAATTY 1 cut(s) 212
AspLEI GCGC 1 cut(s) 378
AspS9I GGNCC 7 cut(s) 120, 194, 195, 219, 300, 365, 382
AsuHPI GGTGA 1 cut(s) 152
AvaII GGWCC 1 cut(s) 219
BaeGI GKGCMC 1 cut(s) 198
BanI GGYRCC 1 cut(s) 375
BanII GRGCYC 2 cut(s) 49, 198
BbsI GAAGAC 1 cut(s) 12
Bbv12I GWGCWC 1 cut(s) 49
BbvI GCAGC 1 cut(s) 217
BccI CCATC 3 cut(s) 81, 131, 206
BceAI ACGGC 1 cut(s) 369
BciT130I CCWGG 1 cut(s) 380
BfoI RGCGCY 1 cut(s) 379
BisI GCNGC 3 cut(s) 82, 206, 312
BlsI GCNGC 3 cut(s) 83, 207, 313
Bme1390I CCNGG 1 cut(s) 380
Bme18I GGWCC 1 cut(s) 219
BmgT120I GGNCC 7 cut(s) 120, 194, 195, 219, 300, 365, 382
BmiI GGNNCC 4 cut(s) 21, 196, 220, 377
BmrFI CCNGG 1 cut(s) 380
BpiI GAAGAC 1 cut(s) 12
BsaHI GRCGYC 1 cut(s) 376
BsaJI CCNNGG 4 cut(s) 116, 243, 258, 379
BsaXI ACNNNNNCTCC 2 cut(s) 323, 353
Bsc4I CCNNNNNNNGG 2 cut(s) 36, 128
Bse118I RCCGGY 1 cut(s) 297
BseBI CCWGG 1 cut(s) 380
BseDI CCNNGG 4 cut(s) 116, 243, 258, 379
BseLI CCNNNNNNNGG 2 cut(s) 36, 128
BseRI GAGGAG 1 cut(s) 232
BseSI GKGCMC 1 cut(s) 198
BseXI GCAGC 1 cut(s) 217
BseYI CCCAGC 1 cut(s) 40
Bsh1285I CGRYCG 2 cut(s) 249, 298
BshNI GGYRCC 1 cut(s) 375
BsiEI CGRYCG 2 cut(s) 249, 298
BsiHKAI GWGCWC 1 cut(s) 49
BsiSI CCGG 1 cut(s) 298
BslFI GGGAC 1 cut(s) 340
BslI CCNNNNNNNGG 2 cut(s) 36, 128
BsmFI GGGAC 1 cut(s) 340
Bsp120I GGGCCC 1 cut(s) 194
Bsp1286I GDGCHC 2 cut(s) 49, 198
BspACI CCGC 7 cut(s) 70, 79, 82, 101, 106, 311, 314
BspLI GGNNCC 4 cut(s) 21, 196, 220, 377
BspT107I GGYRCC 1 cut(s) 375
BsrFI RCCGGY 1 cut(s) 297
BssAI RCCGGY 1 cut(s) 297
BssECI CCNNGG 4 cut(s) 116, 243, 258, 379
BssNI GRCGYC 1 cut(s) 376
BssT1I CCWWGG 1 cut(s) 116
Bst2UI CCWGG 1 cut(s) 380
Bst4CI ACNGT 1 cut(s) 164
BstACI GRCGYC 1 cut(s) 376
BstH2I RGCGCY 1 cut(s) 379
BstHHI GCGC 1 cut(s) 378
BstMCI CGRYCG 2 cut(s) 249, 298
BstMWI GCNNNNNNNGC 2 cut(s) 78, 202
BstNI CCWGG 1 cut(s) 380
BstSCI CCNGG 1 cut(s) 378
BstSLI GKGCMC 1 cut(s) 198
BstV1I GCAGC 1 cut(s) 217
BstV2I GAAGAC 1 cut(s) 12
BstXI CCANNNNNNTGG 3 cut(s) 117, 191, 235
BtsI GCAGTG 1 cut(s) 30
BtsIMutI CAGTG 1 cut(s) 30
CfoI GCGC 1 cut(s) 378
Cfr10I RCCGGY 1 cut(s) 297
Cfr13I GGNCC 7 cut(s) 120, 194, 195, 219, 300, 365, 382
Csp6I GTAC 1 cut(s) 322
CviAII CATG 3 cut(s) 36, 173, 202
CviQI GTAC 1 cut(s) 322
DinI GGCGCC 1 cut(s) 377
EaeI YGGCCR 2 cut(s) 255, 291
EciI GGCGGA 1 cut(s) 68
Ecl136II GAGCTC 1 cut(s) 47
Eco130I CCWWGG 1 cut(s) 116
Eco147I AGGCCT 1 cut(s) 263
Eco24I GRGCYC 2 cut(s) 49, 198
Eco47I GGWCC 1 cut(s) 219
Eco53kI GAGCTC 1 cut(s) 47
EcoICRI GAGCTC 1 cut(s) 47
EcoO109I RGGNCCY 1 cut(s) 219
EcoRII CCWGG 1 cut(s) 378
EcoT14I CCWWGG 1 cut(s) 116
EcoT38I GRGCYC 2 cut(s) 49, 198
EgeI GGCGCC 1 cut(s) 377
EheI GGCGCC 1 cut(s) 377
ErhI CCWWGG 1 cut(s) 116
FaeI CATG 3 cut(s) 39, 176, 205
FaiI YATR 8 cut(s) 37, 146, 174, 190, 192, 203, 363, 370
FaqI GGGAC 1 cut(s) 340
FatI CATG 3 cut(s) 35, 172, 201
FauI CCCGC 3 cut(s) 77, 113, 307
Fnu4HI GCNGC 3 cut(s) 82, 206, 312
FriOI GRGCYC 2 cut(s) 49, 198
Fsp4HI GCNGC 3 cut(s) 82, 206, 312
GlaI GCGC 1 cut(s) 377
GluI GCNGC 3 cut(s) 82, 206, 312
GsaI CCCAGC 1 cut(s) 44
HaeII RGCGCY 1 cut(s) 379
HapII CCGG 1 cut(s) 298
HhaI GCGC 1 cut(s) 378
Hin1I GRCGYC 1 cut(s) 376
Hin1II CATG 3 cut(s) 39, 176, 205
Hin6I GCGC 1 cut(s) 376
HinP1I GCGC 1 cut(s) 376
HpaII CCGG 1 cut(s) 298
HphI GGTGA 1 cut(s) 152
Hpy166II GTNNAC 1 cut(s) 324
Hpy188I TCNGA 1 cut(s) 343
Hpy8I GTNNAC 1 cut(s) 324
Hpy99I CGWCG 1 cut(s) 284
HpyAV CCTTC 2 cut(s) 232, 274
HpyCH4III ACNGT 1 cut(s) 164
HpyCH4IV ACGT 1 cut(s) 282
HpyCH4V TGCA 3 cut(s) 35, 176, 205
HpyF10VI GCNNNNNNNGC 2 cut(s) 78, 202
HpySE526I ACGT 1 cut(s) 282
Hsp92I GRCGYC 1 cut(s) 376
Hsp92II CATG 3 cut(s) 39, 176, 205
HspAI GCGC 1 cut(s) 376
KasI GGCGCC 1 cut(s) 375
LmnI GCTCC 1 cut(s) 44
LpnPI CCDG 4 cut(s) 26, 74, 311, 365
Lsp1109I GCAGC 1 cut(s) 217
MaeII ACGT 1 cut(s) 282
MaeIII GTNAC 1 cut(s) 283
MboII GAAGA 1 cut(s) 17
MhlI GDGCHC 2 cut(s) 49, 198
MluCI AATT 2 cut(s) 94, 212
Mly113I GGCGCC 1 cut(s) 376
MmeI TCCRAC 1 cut(s) 366
MnlI CCTC 8 cut(s) 146, 152, 164, 168, 250, 253, 253, 278
MseI TTAA 1 cut(s) 388
MslI CAYNNNNRTG 1 cut(s) 233
MspA1I CMGCKG 1 cut(s) 208
MspI CCGG 1 cut(s) 298
MspR9I CCNGG 1 cut(s) 380
MvaI CCWGG 1 cut(s) 380
MwoI GCNNNNNNNGC 2 cut(s) 78, 202
NarI GGCGCC 1 cut(s) 376
NlaIII CATG 3 cut(s) 39, 176, 205
NlaIV GGNNCC 4 cut(s) 21, 196, 220, 377
NmeAIII GCCGAG 1 cut(s) 283
PceI AGGCCT 1 cut(s) 263
PcsI WCGNNNNNNNCGW 1 cut(s) 255
PflMI CCANNNNNTGG 1 cut(s) 36
PkrI GCNGC 3 cut(s) 83, 207, 313
PluTI GGCGCC 1 cut(s) 379
PpuMI RGGWCCY 1 cut(s) 219
Psp124BI GAGCTC 1 cut(s) 49
Psp5II RGGWCCY 1 cut(s) 219
Psp6I CCWGG 1 cut(s) 378
PspFI CCCAGC 1 cut(s) 40
PspGI CCWGG 1 cut(s) 378
PspN4I GGNNCC 4 cut(s) 21, 196, 220, 377
PspOMI GGGCCC 1 cut(s) 194
PspPI GGNCC 7 cut(s) 120, 194, 195, 219, 300, 365, 382
PspPPI RGGWCCY 1 cut(s) 219
PvuII CAGCTG 1 cut(s) 208
RsaI GTAC 1 cut(s) 323
RsaNI GTAC 1 cut(s) 322
RseI CAYNNNNRTG 1 cut(s) 233
SacI GAGCTC 1 cut(s) 49
SaqAI TTAA 1 cut(s) 388
SatI GCNGC 3 cut(s) 82, 206, 312
Sau96I GGNCC 7 cut(s) 120, 194, 195, 219, 300, 365, 382
ScrFI CCNGG 1 cut(s) 380
SduI GDGCHC 2 cut(s) 49, 198
SetI ASST 9 cut(s) 49, 57, 118, 138, 144, 156, 210, 285, 332
SfoI GGCGCC 1 cut(s) 377
SinI GGWCC 1 cut(s) 219
SmiMI CAYNNNNRTG 1 cut(s) 233
Sse9I AATT 2 cut(s) 94, 212
SseBI AGGCCT 1 cut(s) 263
SsiI CCGC 7 cut(s) 70, 79, 82, 101, 106, 311, 314
SspDI GGCGCC 1 cut(s) 375
SstI GAGCTC 1 cut(s) 49
StuI AGGCCT 1 cut(s) 263
StyD4I CCNGG 1 cut(s) 378
StyI CCWWGG 1 cut(s) 116
TaaI ACNGT 1 cut(s) 164
TaiI ACGT 1 cut(s) 285
TaqI TCGA 1 cut(s) 249
TaqII GACCGA 1 cut(s) 235
TasI AATT 2 cut(s) 94, 212
TatI WGTACW 1 cut(s) 321
TauI GCSGC 2 cut(s) 84, 314
Tru1I TTAA 1 cut(s) 388
Tru9I TTAA 1 cut(s) 388
TscAI CASTG 1 cut(s) 37
TseI GCWGC 1 cut(s) 205
TspRI CASTG 1 cut(s) 37
Van91I CCANNNNNTGG 1 cut(s) 36
VpaK11BI GGWCC 1 cut(s) 219
XapI RAATTY 1 cut(s) 212
XcmI CCANNNNNNNNNTGG 1 cut(s) 37
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.